##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931031_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3304447 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36866743512606 31.0 31.0 34.0 30.0 34.0 2 31.528143135598786 31.0 31.0 34.0 30.0 34.0 3 31.599912481574073 31.0 31.0 34.0 30.0 34.0 4 35.3744732477174 37.0 35.0 37.0 33.0 37.0 5 35.25553776471524 37.0 35.0 37.0 33.0 37.0 6 35.27315705169428 37.0 35.0 37.0 32.0 37.0 7 35.151849613566206 37.0 35.0 37.0 32.0 37.0 8 35.1533590945777 37.0 35.0 37.0 32.0 37.0 9 36.658592496717304 39.0 35.0 39.0 32.0 39.0 10-11 36.60201994463824 39.0 35.0 39.0 32.0 39.0 12-13 36.49350783958708 38.0 35.0 39.0 32.0 39.0 14-15 37.56778214327541 39.0 36.0 41.0 32.0 41.0 16-17 37.52959602620348 39.0 36.0 41.0 32.0 41.0 18-19 37.55601391095091 39.0 36.0 41.0 32.0 41.0 20-21 37.54118026405023 39.0 36.0 41.0 32.0 41.0 22-23 37.44424800881963 39.0 36.0 41.0 32.0 41.0 24-25 37.32960492330487 39.0 36.0 41.0 31.5 41.0 26-27 37.08971289295909 39.0 36.0 40.0 31.0 41.0 28-29 36.91041390586685 39.0 36.0 40.0 31.0 41.0 30-31 36.68964731466415 38.5 35.5 40.0 30.0 41.0 32-33 36.53609166677511 38.0 35.0 40.0 30.0 41.0 34-35 36.60998663316434 38.5 35.0 40.0 30.0 41.0 36-37 36.68078834370774 39.0 35.0 40.0 30.0 41.0 38-39 36.66787892194972 39.0 35.0 40.0 30.0 41.0 40-41 36.56377057946458 39.0 35.0 40.0 30.0 41.0 42-43 36.43504450215119 39.0 35.0 40.0 30.0 41.0 44-45 36.28365850624931 39.0 35.0 40.0 30.0 41.0 46-47 36.070215682079336 38.5 35.0 40.0 29.5 41.0 48-49 35.874487470974714 38.5 35.0 40.0 29.0 41.0 50-51 35.63014764648972 38.0 35.0 40.0 27.5 41.0 52-53 35.43576156615615 38.0 34.0 40.0 27.0 41.0 54-55 35.30419174524512 38.0 34.0 40.0 27.0 41.0 56-57 35.1511971594642 38.0 34.0 40.0 27.0 41.0 58-59 34.96229656580965 38.0 34.0 40.0 26.5 41.0 60-61 34.67441087722091 38.0 33.5 40.0 26.0 41.0 62-63 34.43624334117025 37.5 33.0 40.0 26.0 41.0 64-65 34.14196838381732 37.0 33.0 40.0 26.0 41.0 66-67 33.84117297084808 36.5 32.5 39.5 25.0 41.0 68-69 33.5191597867964 36.0 32.0 39.0 24.5 40.5 70-71 33.16605274649586 36.0 32.0 39.0 23.5 40.0 72-73 32.75786493171172 35.0 31.5 38.5 22.5 40.0 74-75 32.24474095060384 35.0 31.0 38.0 22.0 40.0 76-77 30.82440390177237 33.5 29.5 36.0 20.5 39.0 78-79 31.57281112391877 34.0 30.5 36.0 21.5 39.0 80-81 31.5363776450341 34.5 31.0 36.0 22.0 39.0 82-83 31.26016788890849 34.0 31.0 36.0 21.0 37.5 84-85 30.916256789713984 34.0 31.0 35.5 20.0 37.0 86-87 30.5669102878636 34.0 30.0 35.0 19.5 37.0 88-89 30.216769704582944 34.0 30.0 35.0 18.0 36.0 90-91 29.882862094625818 34.0 30.0 35.0 17.0 36.0 92-93 29.57273743534092 34.0 29.5 35.0 9.5 36.0 94-95 29.203318739867818 34.0 29.0 35.0 4.5 35.0 96-97 28.79843208258447 34.0 29.0 35.0 2.0 35.0 98-99 28.328455563063955 34.0 29.0 35.0 2.0 35.0 100-101 27.233550122002256 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36388508749794113 1101 2 0.3766902914887318 1101 3 0.2960352597956586 1101 4 0.13202540083391767 1101 5 0.2643707050147839 1101 6 0.26525940186142805 1101 7 0.23311698834915262 1101 8 0.3280725082615277 1101 9 0.42868487254122556 1101 10-11 0.4191150012859737 1101 12-13 0.4312476560297611 1101 14-15 0.5046854037572501 1101 16-17 0.5373315079736116 1101 18-19 0.3737096364733645 1101 20-21 0.34157292524531613 1101 22-23 0.4432316929087534 1101 24-25 0.45185342373272164 1101 26-27 0.48989435641409074 1101 28-29 0.5635605280706457 1101 30-31 0.4670563008781059 1101 32-33 0.49762601261110717 1101 34-35 0.49740486153668684 1101 36-37 0.6002017432771325 1101 38-39 0.6886073968568382 1101 40-41 0.7015667349135057 1101 42-43 0.6431330386111185 1101 44-45 0.6585176626265081 1101 46-47 0.4759296815547245 1101 48-49 0.6595251494379895 1101 50-51 0.6844398645168113 1101 52-53 0.5525834066071837 1101 54-55 0.5842694794755161 1101 56-57 0.5990646691792421 1101 58-59 0.572122469641279 1101 60-61 0.5181218159587573 1101 62-63 0.3295402968231045 1101 64-65 0.3642517694024079 1101 66-67 0.4227034622613388 1101 68-69 0.23436096255661454 1101 70-71 0.3378746631932188 1101 72-73 0.415817725585093 1101 74-75 0.6116413408330814 1101 76-77 0.5613593330477578 1101 78-79 0.5455513591237313 1101 80-81 0.531572610936518 1101 82-83 0.5352142089709346 1101 84-85 0.5645874711834118 1101 86-87 0.5296231596977385 1101 88-89 0.5729484448845739 1101 90-91 0.4678848593367313 1101 92-93 0.33670379126564143 1101 94-95 0.39997529417094313 1101 96-97 0.5961222950823846 1101 98-99 0.603546983729295 1101 100-101 0.7175934207691093 1102 1 0.31518423641837146 1102 2 0.41670683558783495 1102 3 0.4546561813557837 1102 4 0.29165174630295354 1102 5 0.2872067738409285 1102 6 0.3411673938045823 1102 7 0.3866539620568119 1102 8 0.3691050685281141 1102 9 0.4454150927593261 1102 10-11 0.5006622780667342 1102 12-13 0.494985815397861 1102 14-15 0.6043578003314138 1102 16-17 0.61141754737681 1102 18-19 0.6048797111571957 1102 20-21 0.5485549890207935 1102 22-23 0.6376433517909419 1102 24-25 0.5489891425099387 1102 26-27 0.4892779878438418 1102 28-29 0.5571856697210933 1102 30-31 0.6090827680740531 1102 32-33 0.6919716800410853 1102 34-35 0.5693980051509016 1102 36-37 0.5999478653335686 1102 38-39 0.6783702311434752 1102 40-41 0.7213687941830855 1102 42-43 0.7402341223841162 1102 44-45 0.8271795409197722 1102 46-47 0.7098004459122862 1102 48-49 0.6781889612387815 1102 50-51 0.6407300688633342 1102 52-53 0.6781822054786844 1102 54-55 0.6958129396805646 1102 56-57 0.7034006018192045 1102 58-59 0.7483351991822573 1102 60-61 0.602820457795822 1102 62-63 0.5966182121321069 1102 64-65 0.7150381185030739 1102 66-67 0.6588972098024186 1102 68-69 0.6203526165293809 1102 70-71 0.676582390834298 1102 72-73 0.6513119637326881 1102 74-75 0.6723449862513391 1102 76-77 0.5764514520711188 1102 78-79 0.746534085335707 1102 80-81 0.659539115861083 1102 82-83 0.6037966453249126 1102 84-85 0.6806921596869451 1102 86-87 0.6855598684308042 1102 88-89 0.8228002373301102 1102 90-91 0.6630315032720766 1102 92-93 0.6527519877234553 1102 94-95 0.753105979103367 1102 96-97 0.8475378747815654 1102 98-99 0.9806650151160028 1102 100-101 1.1655114535008693 1103 1 0.27385716998183085 1103 2 0.34038428790787734 1103 3 0.3419608886851542 1103 4 0.1939282675760552 1103 5 0.2331355655603673 1103 6 0.2900016711114404 1103 7 0.3320663838939737 1103 8 0.24717628346613907 1103 9 0.45063379891365685 1103 10-11 0.333380572290217 1103 12-13 0.3729497618784734 1103 14-15 0.5151927883230059 1103 16-17 0.5931755434122863 1103 18-19 0.6134839479699963 1103 20-21 0.46884732677413155 1103 22-23 0.5323575716438569 1103 24-25 0.4902618673617667 1103 26-27 0.5137060697107287 1103 28-29 0.4261564584147166 1103 30-31 0.46155156535247244 1103 32-33 0.5822497375249966 1103 34-35 0.5865126500831508 1103 36-37 0.5664969999459188 1103 38-39 0.597328953175861 1103 40-41 0.6742328786397209 1103 42-43 0.7522205611212627 1103 44-45 0.6812050623243664 1103 46-47 0.7965491298140179 1103 48-49 0.6715096481563521 1103 50-51 0.5350241608595638 1103 52-53 0.6300971430066653 1103 54-55 0.7796849315451411 1103 56-57 0.7509648006178651 1103 58-59 0.8027635390260386 1103 60-61 0.7588425123238167 1103 62-63 0.45001035864967065 1103 64-65 0.42885045113016673 1103 66-67 0.47240587865827877 1103 68-69 0.4526434180719221 1103 70-71 0.5730264289882498 1103 72-73 0.5892772999598037 1103 74-75 0.6500573220407162 1103 76-77 0.7426494884508514 1103 78-79 0.8078476204679994 1103 80-81 0.7576967863463615 1103 82-83 0.7731710846412518 1103 84-85 0.6817612242627256 1103 86-87 0.6893349025919342 1103 88-89 0.6509011196110883 1103 90-91 0.708654675588992 1103 92-93 0.5490030425160803 1103 94-95 0.7142978073939474 1103 96-97 0.8710735646367489 1103 98-99 0.7051881323423714 1103 100-101 1.0674203160890592 1104 1 0.2817774455191504 1104 2 0.34939346270787297 1104 3 0.3308222126629019 1104 4 0.18938639240795396 1104 5 0.3253766640901645 1104 6 0.307162321683343 1104 7 0.2981018881174151 1104 8 0.2381787610936854 1104 9 0.4352886769131388 1104 10-11 0.35143769634733957 1104 12-13 0.3978124259657463 1104 14-15 0.4998918250047666 1104 16-17 0.5119714233389203 1104 18-19 0.3534691887641941 1104 20-21 0.4630671200054266 1104 22-23 0.4196078792076605 1104 24-25 0.36068414088860123 1104 26-27 0.4244561493289538 1104 28-29 0.4609958104143814 1104 30-31 0.45099153280924753 1104 32-33 0.46080721360598176 1104 34-35 0.5300458702298982 1104 36-37 0.5746788122102018 1104 38-39 0.46013600368206653 1104 40-41 0.4105103973541233 1104 42-43 0.4069370338526923 1104 44-45 0.5304528659824115 1104 46-47 0.34865520965693264 1104 48-49 0.29056229936890077 1104 50-51 0.31879649203376914 1104 52-53 0.2661096212296883 1104 54-55 0.351330525131452 1104 56-57 0.5093523585896946 1104 58-59 0.43662055923994103 1104 60-61 0.455132556192396 1104 62-63 0.3850358200074524 1104 64-65 0.3526567543541361 1104 66-67 0.30779090982129276 1104 68-69 0.20275353351211578 1104 70-71 0.33629101005254114 1104 72-73 0.3024979995892281 1104 74-75 0.35029380203040716 1104 76-77 0.3347934298434794 1104 78-79 0.4851148754831449 1104 80-81 0.4344891044875254 1104 82-83 0.38419561509061495 1104 84-85 0.5038996552625541 1104 86-87 0.4887032445857038 1104 88-89 0.5580630551644852 1104 90-91 0.45818084368658774 1104 92-93 0.5915039861618077 1104 94-95 0.6717595357993282 1104 96-97 0.6627969498292785 1104 98-99 0.8010467470905347 1104 100-101 0.7805101870838307 1105 1 0.26666731980896685 1105 2 0.292858660641393 1105 3 0.2897732030623992 1105 4 0.16850448893603698 1105 5 0.22369369732206934 1105 6 0.328831001872814 1105 7 0.21064523090357312 1105 8 0.29829824529354454 1105 9 0.3562558704787122 1105 10-11 0.311830069812558 1105 12-13 0.33608125175341286 1105 14-15 0.37281266916200195 1105 16-17 0.37673728544221063 1105 18-19 0.4352829206644486 1105 20-21 0.4329478504945854 1105 22-23 0.3455268555704052 1105 24-25 0.36942206219141127 1105 26-27 0.38568366309344526 1105 28-29 0.40803210652128286 1105 30-31 0.2735964913449962 1105 32-33 0.4207313307949576 1105 34-35 0.4325503152685286 1105 36-37 0.40090303732920773 1105 38-39 0.4453636423565399 1105 40-41 0.4057507417153161 1105 42-43 0.3655342557767369 1105 44-45 0.391773906092169 1105 46-47 0.41231772631973485 1105 48-49 0.5325260403446421 1105 50-51 0.27763354111363014 1105 52-53 0.2176672291255599 1105 54-55 0.3965241648715647 1105 56-57 0.4060235857716705 1105 58-59 0.44739720653905835 1105 60-61 0.40471240977448275 1105 62-63 0.31913690730976185 1105 64-65 0.25890205803175803 1105 66-67 0.3533844566596045 1105 68-69 0.17367773072155757 1105 70-71 0.13834175863412668 1105 72-73 0.29375769718998157 1105 74-75 0.35769019018035664 1105 76-77 0.45004294728111205 1105 78-79 0.4540934046291305 1105 80-81 0.4466543994214476 1105 82-83 0.41171066771870457 1105 84-85 0.3836395302354738 1105 86-87 0.49015868002383556 1105 88-89 0.4995695085213434 1105 90-91 0.5494794195176773 1105 92-93 0.4464516117988033 1105 94-95 0.4685704279493237 1105 96-97 0.4483347946247278 1105 98-99 0.515187024898129 1105 100-101 0.5062406664844197 1106 1 0.18203270344712763 1106 2 0.19911538882098156 1106 3 0.21302766799377792 1106 4 0.01742356315416771 1106 5 0.13219670912301495 1106 6 0.14444319292380214 1106 7 0.2545269947517923 1106 8 0.21744915586983637 1106 9 0.2903155279816332 1106 10-11 0.241774954441631 1106 12-13 0.21370312121988633 1106 14-15 0.277035483798457 1106 16-17 0.2788869412643109 1106 18-19 0.3497981594781834 1106 20-21 0.278575716200713 1106 22-23 0.3254666586404511 1106 24-25 0.35950297624660976 1106 26-27 0.3678407848984122 1106 28-29 0.4529352246440723 1106 30-31 0.3474442869256009 1106 32-33 0.35273858569768635 1106 34-35 0.3574902160608602 1106 36-37 0.33826035301519397 1106 38-39 0.3896861728165817 1106 40-41 0.36849744163362175 1106 42-43 0.46047341387966867 1106 44-45 0.4215622005255142 1106 46-47 0.2857523129739974 1106 48-49 0.31156222495376795 1106 50-51 0.33125849078665226 1106 52-53 0.3096602165741018 1106 54-55 0.33894009438643025 1106 56-57 0.35526830974529133 1106 58-59 0.3949468787785051 1106 60-61 0.4457606963118721 1106 62-63 0.3241099164135761 1106 64-65 0.24250914407036817 1106 66-67 0.18792592413566922 1106 68-69 0.07250514143269271 1106 70-71 0.18939180417271473 1106 72-73 0.35760095845638773 1106 74-75 0.42429500515297747 1106 76-77 0.4220308922595528 1106 78-79 0.30737910160403814 1106 80-81 0.26069978947019123 1106 82-83 0.19387740539690057 1106 84-85 0.36497254043377225 1106 86-87 0.31433963043172497 1106 88-89 0.19575966827774138 1106 90-91 0.32556353104617486 1106 92-93 0.3816342547567366 1106 94-95 0.4241699151027518 1106 96-97 0.36516171281553866 1106 98-99 0.29359696599113505 1106 100-101 0.5522203715194642 1107 1 0.18688489737513692 1107 2 0.2570127761323775 1107 3 0.144107169403064 1107 4 0.14333559698512488 1107 5 0.13531247207230024 1107 6 0.28595104621585676 1107 7 0.2503765183155693 1107 8 0.22506126179142427 1107 9 0.24075895321791307 1107 10-11 0.25919655763676275 1107 12-13 0.26468376283428796 1107 14-15 0.21437689338321775 1107 16-17 0.2351084593422712 1107 18-19 0.3919843994814727 1107 20-21 0.37277965140369673 1107 22-23 0.3205288951161265 1107 24-25 0.2856073895628981 1107 26-27 0.3689160990917273 1107 28-29 0.41396817993712176 1107 30-31 0.45727118673055145 1107 32-33 0.4054067112336668 1107 34-35 0.3254687002605934 1107 36-37 0.3211203416547974 1107 38-39 0.4207835057752902 1107 40-41 0.30515175934129246 1107 42-43 0.24631744133245093 1107 44-45 0.3349463072327552 1107 46-47 0.388426351158472 1107 48-49 0.19971663930750339 1107 50-51 0.2639541811211217 1107 52-53 0.338255867392121 1107 54-55 0.3183972290108841 1107 56-57 0.24421141524612722 1107 58-59 0.17524740824915597 1107 60-61 0.2209712388605496 1107 62-63 0.10105591489999455 1107 64-65 0.15282421179202998 1107 66-67 0.2000599581048519 1107 68-69 0.22661621841304225 1107 70-71 0.3268017552381366 1107 72-73 0.3200810305688506 1107 74-75 0.3646672321169291 1107 76-77 0.34721318982115434 1107 78-79 0.42120195335084887 1107 80-81 0.38341458592765676 1107 82-83 0.39773175798763205 1107 84-85 0.4658030881400279 1107 86-87 0.4683292563077224 1107 88-89 0.5240133761245751 1107 90-91 0.6351275364021909 1107 92-93 0.3817635451905552 1107 94-95 0.6516646432511841 1107 96-97 0.6484235197427601 1107 98-99 0.6842451841544488 1107 100-101 0.977133128752822 1108 1 0.12836694981884733 1108 2 0.13159643256475917 1108 3 0.1725208155607021 1108 4 0.06094545517201766 1108 5 0.12866434993815545 1108 6 0.10520354899328765 1108 7 0.085004721828156 1108 8 0.127131904708925 1108 9 0.29303250105035517 1108 10-11 0.20725923898134369 1108 12-13 0.19809295196456134 1108 14-15 0.2161943013801988 1108 16-17 0.21207444136108933 1108 18-19 0.1840588538725143 1108 20-21 0.14702704000313815 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-1.4546295650571253 2316 90-91 -1.3797614182543931 2316 92-93 -1.4100769981927819 2316 94-95 -1.9389994948874616 2316 96-97 -1.7041233471507518 2316 98-99 -1.7201033859735801 2316 100-101 -2.1234401202505673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 29.0 5 67.0 6 116.0 7 394.0 8 997.0 9 1921.0 10 3581.0 11 5683.0 12 8051.0 13 10730.0 14 12978.0 15 15866.0 16 18779.0 17 21766.0 18 18567.0 19 14763.0 20 14360.0 21 14966.0 22 17072.0 23 20032.0 24 24627.0 25 31691.0 26 41493.0 27 53644.0 28 68895.0 29 87345.0 30 108727.0 31 132649.0 32 160729.0 33 196914.0 34 243643.0 35 304942.0 36 398726.0 37 555321.0 38 572804.0 39 121542.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.30048076777579 22.713962239483244 14.999978815470033 22.985578177270934 2 14.405578828922893 22.586697076392937 41.43718642239618 21.570537672287987 3 17.56679627257915 27.65759707733018 31.854274453028346 22.92133219706232 4 11.80496062385826 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20.491901879274838 24-25 20.292398651869146 28.685533074266733 30.822499634581124 20.199568639282997 26-27 20.321674171630676 28.844119534488964 30.639500406809955 20.194705887070405 28-29 20.285830190218054 28.893010610406684 30.638432571647066 20.1827266277282 30-31 20.64020390841614 28.66740299588845 30.50013036326029 20.192262732435122 32-33 20.528532433497574 28.875607876468727 30.30953950614369 20.286320183890012 34-35 20.591196504715732 28.72952724346242 30.55065618299304 20.128620068828806 36-37 20.659354028064264 28.67895567628289 30.571256766702497 20.09043352895035 38-39 20.458204787831644 28.80379562101228 30.6248608879055 20.113138703250584 40-41 20.620226144511026 28.768746076244295 30.471570938249904 20.13945684099478 42-43 20.57142182650873 28.750808849922855 30.244752387507056 20.43301693606136 44-45 20.480092342561488 29.045203881038738 30.126125133673813 20.348578642725958 46-47 20.650680220688677 28.89109523089641 29.87349246537231 20.5847320830426 48-49 20.684302358134378 28.919386397788198 29.85340917861018 20.542902065467246 50-51 20.719405569623785 29.075417952225497 29.58715008681739 20.61802639133333 52-53 20.699393603371462 29.16575396627023 29.42432463957802 20.710527790780286 54-55 20.696938145453224 29.100330520478952 29.541357434289385 20.661373899778443 56-57 20.763087024793776 29.18351940001873 29.418142436233 20.63525113895449 58-59 20.66126984064527 29.147355264175445 29.60079519216261 20.590579703016672 60-61 20.69341649135873 29.26517922313865 29.520513225283246 20.520891060219373 62-63 20.68625351350466 29.123427226999247 29.50189682232966 20.688422437166434 64-65 20.446679022676904 29.071601911374373 29.500907285480416 20.98081178046831 66-67 20.565201337730947 29.047317241444176 29.40370063491553 20.98378078590935 68-69 20.511636161750385 29.12071209487317 29.39143374623429 20.976217997142154 70-71 20.492801823940372 28.837856833664027 29.501085816018268 21.168255526377337 72-73 20.615059767755163 28.986910368297163 29.462803285116085 20.935226578831585 74-75 20.514687327440992 29.214172634043873 29.185690676840252 21.08544936167488 76-77 20.41954351554598 29.17425780710946 29.25846676907819 21.14773190826637 78-79 20.358810622682906 29.13107361731104 29.321873344934552 21.1882424150715 80-81 20.43450873512931 29.141115851696103 29.23772815672952 21.18664725644507 82-83 20.49642496914007 29.02676302570445 29.381255017859264 21.09555698729621 84-85 20.641396275988086 29.07280703851507 29.053514854376544 21.232281831120307 86-87 20.3814889450489 29.13100134455175 29.190421271698412 21.29708843870094 88-89 20.456724338496883 29.03564109423552 29.070851253645092 21.436783313622502 90-91 20.404316632982695 28.98888761779555 29.261249630021048 21.345546119200712 92-93 20.431193599139903 29.008165128339858 29.12754259784019 21.43309867468005 94-95 20.134077481919334 29.01015870556399 29.18238580686475 21.67337800565193 96-97 20.187017761376303 28.880791043459485 29.172685704757424 21.759505490406784 98-99 20.10039803936937 29.003884764984882 29.020180986410132 21.875536209235616 100-101 20.123836151707078 28.9014319188657 28.971625206880304 22.00310672254692 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 153.0 1 112.5 2 100.0 3 143.0 4 136.5 5 146.5 6 251.0 7 497.5 8 876.0 9 1163.0 10 1183.0 11 1189.0 12 1399.0 13 1632.5 14 1867.5 15 2211.5 16 2579.0 17 2997.0 18 3491.0 19 4055.5 20 4767.0 21 5793.0 22 7188.5 23 9299.5 24 12188.5 25 16319.5 26 22520.5 27 30560.0 28 39455.0 29 50872.5 30 63073.0 31 74417.0 32 86838.5 33 97370.5 34 105887.5 35 114186.5 36 124397.0 37 134543.5 38 137668.5 39 136153.0 40 135470.0 41 135243.0 42 134917.0 43 135019.5 44 134861.0 45 135302.5 46 134332.0 47 131147.5 48 127029.5 49 119997.5 50 111207.5 51 101907.5 52 91877.0 53 81616.5 54 71142.5 55 58906.0 56 46552.0 57 35483.5 58 26214.5 59 19456.5 60 13560.0 61 9079.5 62 6231.5 63 3827.0 64 2037.5 65 1107.5 66 591.5 67 325.5 68 174.0 69 88.0 70 48.0 71 29.5 72 18.5 73 13.5 74 8.0 75 5.0 76 5.0 77 2.5 78 2.0 79 1.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004509075194730011 2 0.007293202160603574 3 0.002148619723663294 4 0.17397767311746867 5 0.002178881973292354 6 4.8419599406496764E-4 7 0.0 8 7.565562407265119E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.2696687221795356E-4 24-25 6.052449925812095E-4 26-27 0.002375586595881247 28-29 7.565562407265119E-5 30-31 0.0066123015439497135 32-33 0.022182228978101327 34-35 0.013693667957149864 36-37 9.381297385008748E-4 38-39 2.2696687221795356E-4 40-41 0.0016795548544128564 42-43 0.011287819111639558 44-45 0.006914924040240318 46-47 0.01015298475054979 48-49 0.010652311869429288 50-51 0.19466192073893152 52-53 0.11505707308968793 54-55 0.01715869553967729 56-57 0.00637020354691723 58-59 0.01790012065558927 60-61 0.12298578249250176 62-63 0.2384967893266256 64-65 0.24438279687947786 66-67 0.25474761737743107 68-69 0.24987539518715232 70-71 0.2374981350888666 72-73 0.16297734537730518 74-75 0.2838145081461437 76-77 0.22067232429510897 78-79 0.005961663176924914 80-81 7.565562407265119E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 9.078674888718142E-5 90-91 0.0062037611739573974 92-93 0.019398102012227764 94-95 0.0026933402169863822 96-97 4.539337444359071E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3304447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.863677800942334 #Duplication Level Percentage of deduplicated Percentage of total 1 72.18521449230208 40.32531564389876 2 15.88551359039634 17.748464258327836 3 5.455933033776885 9.143654553072892 4 2.427163966043145 5.423612230763665 5 1.256561133437861 3.5098063147779786 6 0.7185376581286413 2.4084093732925242 7 0.47849686603586344 1.8711416327091703 8 0.3100443151292234 1.3856172579514217 9 0.2164180846198794 1.0880919134551814 >10 0.9642452293113042 9.41207047649171 >50 0.06328433827498185 2.455405900036902 >100 0.03636442349424201 3.7498787721260327 >500 0.0015180569141526306 0.5517727448198257 >1k 5.96379499217427E-4 0.5526510723962933 >5k 1.0843263622135038E-4 0.37410785587981665 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6893 0.20859768669311388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5459 0.16520162072504113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3503 0.10600866045059884 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.0262249629060474E-5 0.0 0.0 0.0 4 0.0 3.0262249629060474E-5 0.0 0.0 0.0 5 0.0 3.0262249629060474E-5 0.0 0.0 0.0 6 0.0 3.0262249629060474E-5 0.0 0.0 0.0 7 0.0 3.0262249629060474E-5 0.0 0.0 0.0 8 0.0 3.0262249629060474E-5 0.0 0.0 0.0 9 0.0 3.0262249629060474E-5 0.0 6.052449925812095E-5 0.0 10-11 3.0262249629060474E-5 3.0262249629060474E-5 0.0 9.078674888718143E-5 0.0 12-13 6.052449925812095E-5 3.0262249629060474E-5 0.0 9.078674888718143E-5 0.0 14-15 6.052449925812095E-5 3.0262249629060474E-5 0.0 9.078674888718143E-5 0.0 16-17 6.052449925812095E-5 3.0262249629060474E-5 0.0 9.078674888718143E-5 0.0 18-19 6.052449925812095E-5 3.0262249629060474E-5 0.0 9.078674888718143E-5 0.0 20-21 6.052449925812095E-5 3.0262249629060474E-5 0.0 1.3618012333077215E-4 0.0 22-23 1.210489985162419E-4 3.0262249629060474E-5 0.0 1.8157349777436286E-4 0.0 24-25 1.9670462258889308E-4 3.0262249629060474E-5 0.0 3.0262249629060475E-4 0.0 26-27 2.57229121847014E-4 3.0262249629060474E-5 0.0 5.14458243694028E-4 0.0 28-29 2.874913714760745E-4 3.0262249629060474E-5 0.0 0.0011348343610897678 0.0 30-31 3.0262249629060475E-4 3.0262249629060474E-5 0.0 0.004599861943617192 0.0 32-33 3.0262249629060475E-4 3.0262249629060474E-5 0.0 0.01404168382788406 0.0 34-35 3.17753621105135E-4 3.0262249629060474E-5 0.0 0.028507039150574967 0.0 36-37 3.328847459196652E-4 3.0262249629060474E-5 0.0 0.053034592474928484 0.0 38-39 3.328847459196652E-4 3.0262249629060474E-5 0.0 0.09452413671637039 0.0 40-41 3.631469955487257E-4 3.0262249629060474E-5 0.0 0.14120365676919616 0.0 42-43 3.631469955487257E-4 3.0262249629060474E-5 0.0 0.191908056022687 0.0 44-45 3.631469955487257E-4 3.0262249629060474E-5 0.0 0.2531739803967199 0.0 46-47 3.631469955487257E-4 3.0262249629060474E-5 0.0 0.32752832773532153 0.0 48-49 3.631469955487257E-4 3.0262249629060474E-5 0.0 0.41221723332224725 0.0 50-51 3.631469955487257E-4 3.0262249629060474E-5 0.0 0.5009612803594672 0.0 52-53 3.7827812036325594E-4 3.0262249629060474E-5 0.0 0.5938512555958682 0.0 54-55 3.9340924517778617E-4 3.0262249629060474E-5 0.0 0.6972876248279969 0.0 56-57 3.9340924517778617E-4 3.0262249629060474E-5 0.0 0.8214536350560321 0.0 58-59 3.9340924517778617E-4 3.0262249629060474E-5 0.0 0.9625816362011557 0.0 60-61 4.236714948068467E-4 3.0262249629060474E-5 0.0 1.1111390196302136 0.0 62-63 4.236714948068467E-4 3.0262249629060474E-5 0.0 1.266626458224326 0.0 64-65 4.236714948068467E-4 3.0262249629060474E-5 0.0 1.4340674854219178 0.0 66-67 4.236714948068467E-4 3.0262249629060474E-5 0.0 1.623040103230586 0.0 68-69 4.5393374443590713E-4 3.0262249629060474E-5 0.0 1.8366159299876803 0.0 70-71 4.5393374443590713E-4 3.0262249629060474E-5 0.0 2.073312115461377 0.0 72-73 4.5393374443590713E-4 3.0262249629060474E-5 0.0 2.321462562419673 0.0 74-75 4.5393374443590713E-4 3.0262249629060474E-5 0.0 2.580704123867019 0.0 76-77 4.5393374443590713E-4 3.0262249629060474E-5 0.0 2.867544856976069 0.0 78-79 4.841959940649676E-4 3.0262249629060474E-5 0.0 3.1905792406414752 0.0 80-81 4.841959940649676E-4 3.0262249629060474E-5 0.0 3.542377892579303 0.0 82-83 4.841959940649676E-4 3.0262249629060474E-5 0.0 3.9128786147878905 1.5131124814530237E-5 84-85 4.841959940649676E-4 3.0262249629060474E-5 0.0 4.289764671668209 3.0262249629060474E-5 86-87 4.841959940649676E-4 3.0262249629060474E-5 0.0 4.695793274941314 3.0262249629060474E-5 88-89 4.841959940649676E-4 3.0262249629060474E-5 0.0 5.14414363432066 3.0262249629060474E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4145 0.0 57.452896 1 GTATCAA 7825 0.0 42.521877 1 ATCAACG 9640 0.0 33.96943 3 CAACGCA 9795 0.0 33.368137 5 TCAACGC 9940 0.0 32.992004 4 TATCAAC 10180 0.0 32.216644 2 AACGCAG 10425 0.0 31.715193 6 ACGCAGA 11720 0.0 27.684656 7 CGCAGAG 11805 0.0 27.2844 8 GCAGAGT 14015 0.0 22.169315 9 AGAGTAC 12575 0.0 20.87919 10-11 GAGTACT 9400 0.0 20.841372 12-13 GTACATG 8395 0.0 20.043856 1 CAGAGTA 13385 0.0 19.952353 10-11 TACATGG 8705 0.0 19.219976 2 ACGGGCG 425 0.0 18.9748 5 GGCGTCG 450 0.0 18.974512 8 ACATGGG 8500 0.0 18.787386 3 GTACTTT 10505 0.0 18.468487 14-15 AGTACTT 10435 0.0 18.205984 12-13 >>END_MODULE