FastQCFastQC Report
Sat 18 Jun 2016
SRR1931030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846366
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN820604.444405930351837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14900.055.0959631
GTATCAA26950.044.549531
TCAACGC33750.036.0083544
CAACGCA33800.035.797115
ATCAACG33250.035.187783
TATCAAC34750.034.3951152
AACGCAG37400.031.4935846
GTACATG36000.028.0769391
CGCAGAG42300.027.216598
ACGCAGA42400.027.0788697
ACATGGG36250.025.4588413
TACATGG37600.025.239432
GAGTACA24000.025.0127051
GTGATCG1601.2323471E-724.9071068
GTGTACG1251.3374665E-424.6278931
GTGGTAT8050.024.2199781
GCAGAGT46250.023.0221169
GCGTGCG2705.456968E-1222.627239
AGTACAT25550.021.281912
TTCGCAC1454.1050132E-420.8679033
Produced by FastQC (version 0.11.5)