##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931030_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846366 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.87592817458727 28.0 2.0 31.0 2.0 31.0 2 20.17565314785909 28.0 2.0 31.0 2.0 33.0 3 20.940773931062424 30.0 2.0 31.0 2.0 33.0 4 22.544315699054252 32.0 2.0 35.0 2.0 37.0 5 21.702659711021543 30.0 2.0 35.0 2.0 37.0 6 22.38690108028419 31.0 2.0 35.0 2.0 37.0 7 22.136197806935353 30.0 2.0 35.0 2.0 37.0 8 21.90223715124737 29.0 2.0 35.0 2.0 37.0 9 20.81071250228828 26.0 2.0 34.0 2.0 37.0 10-11 20.85471109195035 26.5 2.0 33.5 2.0 37.0 12-13 20.517510883540965 26.0 2.0 33.0 2.0 37.0 14-15 22.018120730126096 28.5 2.0 35.0 2.0 37.5 16-17 23.206262463671884 31.5 2.0 36.5 2.0 38.5 18-19 23.275570228221273 32.0 2.0 37.5 2.0 39.5 20-21 23.519700048636075 32.0 2.0 38.0 2.0 40.0 22-23 23.460867184512715 32.0 2.0 38.0 2.0 40.0 24-25 23.51481315188863 32.0 2.0 38.0 2.0 40.0 26-27 23.361008868230893 32.0 2.0 38.0 2.0 40.0 28-29 23.308835301343287 32.0 2.0 38.0 2.0 40.0 30-31 23.481034096165118 32.0 2.0 38.0 2.0 40.0 32-33 23.43443445124098 31.5 2.0 38.0 2.0 40.0 34-35 23.190632301504685 31.0 2.0 38.0 2.0 40.0 36-37 22.690209037644756 30.0 2.0 38.0 2.0 40.0 38-39 22.712592194613634 30.0 2.0 38.0 2.0 40.0 40-41 22.721021725919996 30.0 2.0 38.0 2.0 40.0 42-43 22.662520594508347 30.0 2.0 38.0 2.0 40.0 44-45 22.326846085770644 30.0 2.0 38.0 2.0 40.0 46-47 22.027397059954527 30.0 2.0 38.0 2.0 40.0 48-49 21.92521498987741 29.5 2.0 38.0 2.0 40.0 50-51 19.15381890697727 17.5 2.0 36.0 2.0 38.5 52-53 21.401775704275316 28.0 2.0 37.0 2.0 39.0 54-55 22.37147699860158 30.5 2.0 38.0 2.0 40.0 56-57 22.429060652113396 31.0 2.0 38.0 2.0 40.0 58-59 22.180213457136883 30.5 2.0 38.0 2.0 40.0 60-61 21.945872053536515 30.0 2.0 38.0 2.0 40.0 62-63 21.535522480374965 28.5 2.0 37.0 2.0 40.0 64-65 21.261336593069846 28.0 2.0 37.0 2.0 40.0 66-67 20.986144404738823 28.0 2.0 36.0 2.0 39.0 68-69 20.594245127997375 27.5 2.0 36.0 2.0 39.0 70-71 20.31583878819259 27.0 2.0 35.5 2.0 39.0 72-73 19.946651151505172 26.0 2.0 35.0 2.0 38.5 74-75 19.542424687196366 26.0 2.0 35.0 2.0 38.0 76-77 19.20272795317938 25.5 2.0 34.0 2.0 37.0 78-79 18.895334673623758 25.0 2.0 34.0 2.0 36.5 80-81 18.522007012694125 24.0 2.0 34.0 2.0 36.0 82-83 18.12085604912569 22.5 2.0 34.0 2.0 35.5 84-85 17.79687640478648 21.0 2.0 34.0 2.0 35.0 86-87 17.35852561193176 19.5 2.0 33.0 2.0 35.0 88-89 17.006448071509116 18.0 2.0 33.0 2.0 35.0 90-91 16.584059715137734 12.0 2.0 32.5 2.0 35.0 92-93 16.072977405346503 4.5 2.0 32.0 2.0 35.0 94-95 15.552143237039678 2.0 2.0 32.0 2.0 34.0 96-97 14.94775927416341 2.0 2.0 31.5 2.0 34.0 98-99 14.26123504223973 2.0 2.0 31.0 2.0 34.0 100-101 13.069867512724997 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.42327136554184 1101 2 10.444723501261482 1101 3 9.774957062680297 1101 4 12.10845472463199 1101 5 12.785423123447043 1101 6 12.056752746519706 1101 7 12.230394463960817 1101 8 12.182576002187517 1101 9 13.26550935630343 1101 10-11 12.580726401026809 1101 12-13 12.080681616478106 1101 14-15 11.465111406868079 1101 16-17 11.036941716867911 1101 18-19 11.669430886404822 1101 20-21 11.865538165313328 1101 22-23 12.075886208442899 1101 24-25 12.289887982797527 1101 26-27 12.494964825041333 1101 28-29 12.456198030487826 1101 30-31 12.153307407633278 1101 32-33 11.969513346673647 1101 34-35 12.054574839281646 1101 36-37 12.607554030958557 1101 38-39 12.34659298384344 1101 40-41 12.047569628904533 1101 42-43 11.884036952931456 1101 44-45 11.734294107358995 1101 46-47 11.780307818864749 1101 48-49 12.03993276925263 1101 50-51 14.00296867906103 1101 52-53 12.631866686817219 1101 54-55 12.206848624765335 1101 56-57 12.255683214509261 1101 58-59 12.262893322584336 1101 60-61 12.119330779353664 1101 62-63 12.341306834127721 1101 64-65 12.01720034905501 1101 66-67 11.724926801283761 1101 68-69 11.901386197097654 1101 70-71 11.323249680546972 1101 72-73 11.28002640027163 1101 74-75 11.18823946867457 1101 76-77 10.975276943570101 1101 78-79 10.608964062073028 1101 80-81 10.59409886814371 1101 82-83 10.373472152678225 1101 84-85 10.291222020026904 1101 86-87 10.093238599090014 1101 88-89 9.953987350405772 1101 90-91 9.901200322778504 1101 92-93 9.700990225748159 1101 94-95 9.583592434299426 1101 96-97 9.676241221824421 1101 98-99 9.315083865566363 1101 100-101 8.308054920902885 1102 1 9.211366024558782 1102 2 9.994984919965816 1102 3 9.414413502971534 1102 4 11.61523952059325 1102 5 12.230651441299567 1102 6 11.270229270852944 1102 7 11.582157054445144 1102 8 11.272006645042751 1102 9 11.936916377985927 1102 10-11 10.96081893439126 1102 12-13 10.50736558453146 1102 14-15 10.068742729875876 1102 16-17 10.024446198030159 1102 18-19 11.225647787310333 1102 20-21 11.626392137767002 1102 22-23 11.934421223366048 1102 24-25 11.835694809909722 1102 26-27 11.88515730062515 1102 28-29 11.849661789659272 1102 30-31 12.011722221969066 1102 32-33 11.865332475015538 1102 34-35 11.685260854259628 1102 36-37 12.01614942734636 1102 38-39 12.057439575559304 1102 40-41 11.708262490395498 1102 42-43 11.263706745750635 1102 44-45 11.344043288800027 1102 46-47 11.24405447820223 1102 48-49 11.189295143797665 1102 50-51 13.346869869960646 1102 52-53 12.028789270609678 1102 54-55 11.643897257501223 1102 56-57 11.497400269355879 1102 58-59 11.43893896225531 1102 60-61 11.263218052290537 1102 62-63 11.31214111055533 1102 64-65 11.261941116454913 1102 66-67 11.237189369977347 1102 68-69 10.883456065270643 1102 70-71 10.73980263698937 1102 72-73 10.771137742567756 1102 74-75 10.625159746865123 1102 76-77 10.5528902519542 1102 78-79 10.022723584982135 1102 80-81 9.754504513494336 1102 82-83 9.77298474281109 1102 84-85 9.684699697161097 1102 86-87 9.448109956344435 1102 88-89 9.28780659402776 1102 90-91 8.965103734928743 1102 92-93 9.006375493233124 1102 94-95 8.82650840213909 1102 96-97 8.691363158642494 1102 98-99 8.348581590190333 1102 100-101 7.668944883240032 1103 1 9.12268501069088 1103 2 10.009701481072057 1103 3 9.25173865754282 1103 4 11.704647244002388 1103 5 12.032722036890977 1103 6 11.115001673850244 1103 7 11.335208015021013 1103 8 10.894970223092244 1103 9 10.746533167581624 1103 10-11 10.110722178365002 1103 12-13 9.735626381794464 1103 14-15 9.876925224324392 1103 16-17 10.460172591610725 1103 18-19 11.426541678848242 1103 20-21 12.071953777259207 1103 22-23 12.458928962185201 1103 24-25 12.56150843635108 1103 26-27 12.466569833288279 1103 28-29 12.377540468522398 1103 30-31 12.163971612497562 1103 32-33 12.360895999185427 1103 34-35 12.441163637041644 1103 36-37 12.617661354584495 1103 38-39 12.45880455336999 1103 40-41 12.026389154039293 1103 42-43 11.7513267692315 1103 44-45 11.803065822893291 1103 46-47 11.703887700856939 1103 48-49 11.572551556413472 1103 50-51 13.872150049470651 1103 52-53 12.599564599291924 1103 54-55 11.978389159484678 1103 56-57 11.924649909327009 1103 58-59 11.932082061397114 1103 60-61 11.867312403689798 1103 62-63 11.878830568887878 1103 64-65 11.603597426632952 1103 66-67 11.53121502487512 1103 68-69 11.263918858961627 1103 70-71 11.212135152889953 1103 72-73 11.160479585405596 1103 74-75 11.153577083239067 1103 76-77 11.036099852502307 1103 78-79 10.516086332221356 1103 80-81 10.333775026825105 1103 82-83 10.174035977219539 1103 84-85 9.952738834156104 1103 86-87 9.972397354169964 1103 88-89 9.698054085466332 1103 90-91 9.449213546228162 1103 92-93 9.358224722344525 1103 94-95 9.29233476124114 1103 96-97 9.200989704216768 1103 98-99 8.746011887603556 1103 100-101 8.19169862460239 1104 1 8.56218811032765 1104 2 9.667170961190202 1104 3 9.23620852435814 1104 4 11.10115534646502 1104 5 11.38770508721559 1104 6 10.513456207186074 1104 7 10.37141295130412 1104 8 10.20795366341914 1104 9 9.71532389610368 1104 10-11 8.879842618560794 1104 12-13 8.788243363198848 1104 14-15 9.174753660778808 1104 16-17 9.927512654870423 1104 18-19 11.068809332786213 1104 20-21 11.610757902469768 1104 22-23 12.0169668651187 1104 24-25 11.865350096526893 1104 26-27 12.235482465065527 1104 28-29 12.123920690999967 1104 30-31 11.772303421810683 1104 32-33 11.826465903789096 1104 34-35 11.735581619231542 1104 36-37 12.136343042214708 1104 38-39 11.75311126372279 1104 40-41 11.381260657101464 1104 42-43 10.97070952452049 1104 44-45 11.220345265035792 1104 46-47 11.377924428275094 1104 48-49 11.173819945186253 1104 50-51 13.166846899816637 1104 52-53 11.915748344349417 1104 54-55 11.462976495207243 1104 56-57 11.412207431909131 1104 58-59 11.349766791926474 1104 60-61 11.119519662359362 1104 62-63 11.234735605535974 1104 64-65 11.165416368625074 1104 66-67 10.96306887427475 1104 68-69 10.65194565123694 1104 70-71 10.272464181508848 1104 72-73 10.266547949279925 1104 74-75 10.16548675033797 1104 76-77 9.943830298129487 1104 78-79 9.85182527637281 1104 80-81 9.62663382616893 1104 82-83 9.532843446093722 1104 84-85 9.18735522725196 1104 86-87 8.94510766069633 1104 88-89 8.946456618498093 1104 90-91 8.753040884286325 1104 92-93 8.236151942851153 1104 94-95 8.174984286903623 1104 96-97 7.592941720715851 1104 98-99 7.352411450448459 1104 100-101 6.802170488802274 1105 1 8.37037220305174 1105 2 9.28405091258578 1105 3 8.78167471298525 1105 4 10.915108679707636 1105 5 10.76972609309983 1105 6 10.585497003310316 1105 7 10.048737317259995 1105 8 9.457950795075064 1105 9 8.625649321078871 1105 10-11 7.932695393530267 1105 12-13 7.492312035891974 1105 14-15 8.46680591960644 1105 16-17 9.758116125113158 1105 18-19 10.690327863489639 1105 20-21 11.640554260713404 1105 22-23 11.866987957453492 1105 24-25 12.05521993240691 1105 26-27 12.250475766481532 1105 28-29 11.962519370174213 1105 30-31 11.78265347452151 1105 32-33 11.478580994027208 1105 34-35 11.752807159394099 1105 36-37 11.836126008626795 1105 38-39 11.613708674421904 1105 40-41 11.261353468711036 1105 42-43 11.088163278142279 1105 44-45 11.320092130335954 1105 46-47 10.906122918752317 1105 48-49 10.765756242866473 1105 50-51 12.858759797145666 1105 52-53 11.566996527620326 1105 54-55 10.977333024238746 1105 56-57 10.786856126465707 1105 58-59 10.877811459114508 1105 60-61 10.934116171731372 1105 62-63 10.831816244960578 1105 64-65 10.59375432732999 1105 66-67 10.168162546387535 1105 68-69 10.217969195138746 1105 70-71 10.133434877171673 1105 72-73 10.012895746805249 1105 74-75 9.879547266261014 1105 76-77 9.383315308963827 1105 78-79 9.312283352255584 1105 80-81 9.037417252316548 1105 82-83 9.069185765748923 1105 84-85 8.806239817627077 1105 86-87 8.621730683173208 1105 88-89 8.230402269915043 1105 90-91 8.0029243494701 1105 92-93 7.725537735662785 1105 94-95 7.466180711906297 1105 96-97 7.387426735552525 1105 98-99 7.059670388808945 1105 100-101 6.5339114775991884 1106 1 8.141493554818254 1106 2 9.295827666298017 1106 3 8.856943530189554 1106 4 10.571534690972356 1106 5 10.800960092075766 1106 6 10.22656203147212 1106 7 9.656520215365472 1106 8 9.042692218526167 1106 9 7.797180299061463 1106 10-11 7.63008402640277 1106 12-13 7.405010448590385 1106 14-15 8.37771221760951 1106 16-17 9.539734148666664 1106 18-19 11.124019619762041 1106 20-21 11.262930092766656 1106 22-23 11.687776487919447 1106 24-25 11.766689466457091 1106 26-27 11.8044952237268 1106 28-29 11.866001193010874 1106 30-31 11.466473238986438 1106 32-33 11.729989084144972 1106 34-35 11.5449218547346 1106 36-37 11.865055860137293 1106 38-39 11.5858028884516 1106 40-41 11.52837855831676 1106 42-43 11.269422878756718 1106 44-45 10.745079329516702 1106 46-47 10.860295985828614 1106 48-49 10.936519171693917 1106 50-51 12.685948737237833 1106 52-53 11.49095966126088 1106 54-55 10.799401636629945 1106 56-57 10.765606766506671 1106 58-59 10.727017808320856 1106 60-61 10.536778742135883 1106 62-63 10.598329301796213 1106 64-65 10.520789657591127 1106 66-67 10.483318716382417 1106 68-69 9.971425416336903 1106 70-71 9.82698326426845 1106 72-73 9.807314589611188 1106 74-75 9.506531393245144 1106 76-77 9.365394162010421 1106 78-79 8.934659184308838 1106 80-81 8.669521707001646 1106 82-83 8.67159231976838 1106 84-85 8.555599776664458 1106 86-87 8.23463390897966 1106 88-89 7.8397212663308125 1106 90-91 7.824224912706146 1106 92-93 7.655645262544509 1106 94-95 7.508423415439321 1106 96-97 7.332639229152116 1106 98-99 6.956751802019388 1106 100-101 6.2702146009990845 1107 1 7.7920534065922595 1107 2 8.630091545563687 1107 3 8.547059334331593 1107 4 10.282344937621282 1107 5 10.512897740848153 1107 6 10.0015375794531 1107 7 9.525213519103207 1107 8 8.139698144362534 1107 9 4.841570853477734 1107 10-11 6.551089021176523 1107 12-13 6.648966421005717 1107 14-15 7.747397423375897 1107 16-17 9.199283895672874 1107 18-19 10.976426690464418 1107 20-21 11.344851719201802 1107 22-23 11.176175406398773 1107 24-25 11.256130276037222 1107 26-27 11.43073623150649 1107 28-29 11.526890698819159 1107 30-31 11.09710101389662 1107 32-33 11.492208893457981 1107 34-35 11.532465533816637 1107 36-37 11.931990581900958 1107 38-39 11.750218238481029 1107 40-41 11.452723520632496 1107 42-43 11.336418439933784 1107 44-45 10.62792160740634 1107 46-47 10.952670882685787 1107 48-49 11.303015798012815 1107 50-51 13.306935208929037 1107 52-53 11.73588583502238 1107 54-55 11.350863255097849 1107 56-57 10.929397446890235 1107 58-59 11.386651516300976 1107 60-61 11.185905961353587 1107 62-63 11.326075381077075 1107 64-65 11.305801512880642 1107 66-67 11.261163792110043 1107 68-69 11.00748329619056 1107 70-71 10.730586895102636 1107 72-73 10.582099481596043 1107 74-75 10.674691531881503 1107 76-77 10.504119908268475 1107 78-79 10.19113347959209 1107 80-81 9.8395705800387 1107 82-83 9.522760384837362 1107 84-85 9.68790341261381 1107 86-87 9.329916826460746 1107 88-89 9.211398284739712 1107 90-91 8.915063969594488 1107 92-93 8.751080988998106 1107 94-95 8.301423963639898 1107 96-97 8.304094177243568 1107 98-99 8.285670079832562 1107 100-101 7.640311990561992 1108 1 6.961309040802952 1108 2 8.216451653848878 1108 3 8.2156962839601 1108 4 9.305306805412592 1108 5 10.327209515811127 1108 6 9.192325078741305 1108 7 8.636509027176121 1108 8 5.5452204942148136 1108 9 5.375564134132244 1108 10-11 4.773240006890859 1108 12-13 4.054402842443803 1108 14-15 6.921907387737409 1108 16-17 8.760807485972347 1108 18-19 8.646153648439522 1108 20-21 9.22272640941063 1108 22-23 10.116437203344024 1108 24-25 9.924175986875536 1108 26-27 8.67563516173621 1108 28-29 10.528875901689737 1108 30-31 10.812066203911286 1108 32-33 11.224038753145852 1108 34-35 10.844298851161302 1108 36-37 10.439768001144923 1108 38-39 10.185538125599304 1108 40-41 10.378530619294217 1108 42-43 9.569091424091663 1108 44-45 9.45899878548644 1108 46-47 10.052891372431578 1108 48-49 10.651682265975367 1108 50-51 12.565822387229723 1108 52-53 11.221911951104225 1108 54-55 10.65460660462334 1108 56-57 10.597590775049056 1108 58-59 10.849154023615146 1108 60-61 10.592254121741671 1108 62-63 10.800343540316021 1108 64-65 10.786010788327125 1108 66-67 10.376138960080858 1108 68-69 10.539357223047265 1108 70-71 10.533848147749104 1108 72-73 9.890843351822078 1108 74-75 9.958122430302833 1108 76-77 9.546570853404077 1108 78-79 9.347701078064773 1108 80-81 9.27411852219426 1108 82-83 9.167923358976243 1108 84-85 9.001923800476714 1108 86-87 8.90041955128924 1108 88-89 8.617113652895508 1108 90-91 8.464980170152945 1108 92-93 8.172976934049062 1108 94-95 8.078224779523776 1108 96-97 7.999012524729865 1108 98-99 7.773720130697052 1108 100-101 7.027511067972975 1109 1 6.457880707705627 1109 2 8.118238802893863 1109 3 8.05205841023594 1109 4 8.926682023141343 1109 5 9.464234249384393 1109 6 8.792982501560285 1109 7 6.922646325062065 1109 8 6.630084989745715 1109 9 4.630341785780175 1109 10-11 3.8861027495005906 1109 12-13 4.705415866317939 1109 14-15 6.883973513309602 1109 16-17 6.427076418023955 1109 18-19 6.4817668626175475 1109 20-21 8.97045884492643 1109 22-23 10.184375795389283 1109 24-25 9.871892327294066 1109 26-27 10.300747330893618 1109 28-29 10.818010395572443 1109 30-31 8.259039177818394 1109 32-33 7.323753039376005 1109 34-35 9.118044342793016 1109 36-37 7.519011722444898 1109 38-39 7.409359669144926 1109 40-41 8.366766753974069 1109 42-43 6.9008305947866155 1109 44-45 7.691663088959498 1109 46-47 7.575923740342802 1109 48-49 8.40049918604954 1109 50-51 10.483615130352742 1109 52-53 10.416526696637902 1109 54-55 10.330690579761573 1109 56-57 9.497187030565946 1109 58-59 9.504482287508711 1109 60-61 9.439843223194178 1109 62-63 10.076024312633074 1109 64-65 9.884180951163597 1109 66-67 9.668764594051005 1109 68-69 9.55260949796288 1109 70-71 9.092557594358908 1109 72-73 8.542670803093785 1109 74-75 7.596950769126291 1109 76-77 7.5187152439660565 1109 78-79 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-20.401396292295967 2313 40-41 -20.41811401720807 2313 42-43 -20.341432215969345 2313 44-45 -20.02066567816379 2313 46-47 -19.722909339312196 2313 48-49 -19.61878036747659 2313 50-51 -16.94180343890144 2313 52-53 -19.131672187177422 2313 54-55 -20.080014646012145 2313 56-57 -20.1263543190028 2313 58-59 -19.862843942831216 2313 60-61 -19.63321613753642 2313 62-63 -19.23295590045673 2313 64-65 -18.965641474001295 2313 66-67 -18.682579901129106 2313 68-69 -18.305584312285728 2313 70-71 -18.03707203527072 2313 72-73 -17.671180034044724 2313 74-75 -17.25921361443535 2313 76-77 -16.92928069718057 2313 78-79 -16.640097600125365 2313 80-81 -16.277482901293286 2313 82-83 -15.879610957241347 2313 84-85 -15.557303449141994 2313 86-87 -15.115340489381836 2313 88-89 -14.758076992757768 2313 90-91 -14.363947130304606 2313 92-93 -13.873540605351037 2313 94-95 -13.36305635926626 2313 96-97 -12.756198456459762 2313 98-99 -12.080626590197712 2313 100-101 -10.901060360598455 2314 1 -18.6030020929032 2314 2 -17.927930654234498 2314 3 -18.66686599361997 2314 4 -20.262054658746024 2314 5 -19.403853015963147 2314 6 -20.083729827206838 2314 7 -19.86933743952444 2314 8 -19.637799332933128 2314 9 -18.582748016350724 2314 10-11 -18.61364357216354 2314 12-13 -18.283929643575515 2314 14-15 -19.770009236563556 2314 16-17 -20.935176245598573 2314 18-19 -20.980435065337804 2314 20-21 -21.207115990182654 2314 22-23 -21.156821566356026 2314 24-25 -21.213597271894162 2314 26-27 -21.050343485950624 2314 28-29 -21.000102237608687 2314 30-31 -21.192000903358675 2314 32-33 -21.141746706945657 2314 34-35 -20.89261014731353 2314 36-37 -20.387888328290767 2314 38-39 -20.401396292295967 2314 40-41 -20.41811401720807 2314 42-43 -20.341432215969345 2314 44-45 -20.02066567816379 2314 46-47 -19.722909339312196 2314 48-49 -19.61878036747659 2314 50-51 -16.94180343890144 2314 52-53 -19.131672187177422 2314 54-55 -20.080014646012145 2314 56-57 -20.1263543190028 2314 58-59 -19.862843942831216 2314 60-61 -19.63321613753642 2314 62-63 -19.23295590045673 2314 64-65 -18.965641474001295 2314 66-67 -18.682579901129106 2314 68-69 -18.305584312285728 2314 70-71 -18.03707203527072 2314 72-73 -17.671180034044724 2314 74-75 -17.25921361443535 2314 76-77 -16.92928069718057 2314 78-79 -16.640097600125365 2314 80-81 -16.277482901293286 2314 82-83 -15.879610957241347 2314 84-85 -15.557303449141994 2314 86-87 -15.115340489381836 2314 88-89 -14.758076992757768 2314 90-91 -14.363947130304606 2314 92-93 -13.873540605351037 2314 94-95 -13.36305635926626 2314 96-97 -12.756198456459762 2314 98-99 -12.080626590197712 2314 100-101 -10.901060360598455 2315 1 -18.6030020929032 2315 2 -17.927930654234498 2315 3 -18.66686599361997 2315 4 -20.262054658746024 2315 5 -19.403853015963147 2315 6 -20.083729827206838 2315 7 -19.86933743952444 2315 8 -19.637799332933128 2315 9 -18.582748016350724 2315 10-11 -18.61364357216354 2315 12-13 -18.283929643575515 2315 14-15 -19.770009236563556 2315 16-17 -20.935176245598573 2315 18-19 -20.980435065337804 2315 20-21 -21.207115990182654 2315 22-23 -21.156821566356026 2315 24-25 -21.213597271894162 2315 26-27 -21.050343485950624 2315 28-29 -21.000102237608687 2315 30-31 -21.192000903358675 2315 32-33 -21.141746706945657 2315 34-35 -20.89261014731353 2315 36-37 -20.387888328290767 2315 38-39 -20.401396292295967 2315 40-41 -20.41811401720807 2315 42-43 -20.341432215969345 2315 44-45 -20.02066567816379 2315 46-47 -19.722909339312196 2315 48-49 -19.61878036747659 2315 50-51 -16.94180343890144 2315 52-53 -19.131672187177422 2315 54-55 -20.080014646012145 2315 56-57 -20.1263543190028 2315 58-59 -19.862843942831216 2315 60-61 -19.63321613753642 2315 62-63 -19.23295590045673 2315 64-65 -18.965641474001295 2315 66-67 -18.682579901129106 2315 68-69 -18.305584312285728 2315 70-71 -18.03707203527072 2315 72-73 -17.671180034044724 2315 74-75 -17.25921361443535 2315 76-77 -16.92928069718057 2315 78-79 -16.640097600125365 2315 80-81 -16.277482901293286 2315 82-83 -15.879610957241347 2315 84-85 -15.557303449141994 2315 86-87 -15.115340489381836 2315 88-89 -14.758076992757768 2315 90-91 -14.363947130304606 2315 92-93 -13.873540605351037 2315 94-95 -13.36305635926626 2315 96-97 -12.756198456459762 2315 98-99 -12.080626590197712 2315 100-101 -10.901060360598455 2316 1 -18.6030020929032 2316 2 -17.927930654234498 2316 3 -18.66686599361997 2316 4 -20.262054658746024 2316 5 -19.403853015963147 2316 6 -20.083729827206838 2316 7 -19.86933743952444 2316 8 -19.637799332933128 2316 9 -18.582748016350724 2316 10-11 -18.61364357216354 2316 12-13 -18.283929643575515 2316 14-15 -19.770009236563556 2316 16-17 -20.935176245598573 2316 18-19 -20.980435065337804 2316 20-21 -21.207115990182654 2316 22-23 -21.156821566356026 2316 24-25 -21.213597271894162 2316 26-27 -21.050343485950624 2316 28-29 -21.000102237608687 2316 30-31 -21.192000903358675 2316 32-33 -21.141746706945657 2316 34-35 -20.89261014731353 2316 36-37 -20.387888328290767 2316 38-39 -20.401396292295967 2316 40-41 -20.41811401720807 2316 42-43 -20.341432215969345 2316 44-45 -20.02066567816379 2316 46-47 -19.722909339312196 2316 48-49 -19.61878036747659 2316 50-51 -16.94180343890144 2316 52-53 -19.131672187177422 2316 54-55 -20.080014646012145 2316 56-57 -20.1263543190028 2316 58-59 -19.862843942831216 2316 60-61 -19.63321613753642 2316 62-63 -19.23295590045673 2316 64-65 -18.965641474001295 2316 66-67 -18.682579901129106 2316 68-69 -18.305584312285728 2316 70-71 -18.03707203527072 2316 72-73 -17.671180034044724 2316 74-75 -17.25921361443535 2316 76-77 -16.92928069718057 2316 78-79 -16.640097600125365 2316 80-81 -16.277482901293286 2316 82-83 -15.879610957241347 2316 84-85 -15.557303449141994 2316 86-87 -15.115340489381836 2316 88-89 -14.758076992757768 2316 90-91 -14.363947130304606 2316 92-93 -13.873540605351037 2316 94-95 -13.36305635926626 2316 96-97 -12.756198456459762 2316 98-99 -12.080626590197712 2316 100-101 -10.901060360598455 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 513166.0 3 8118.0 4 26939.0 5 13891.0 6 12254.0 7 12480.0 8 10194.0 9 10034.0 10 11301.0 11 12673.0 12 14434.0 13 15502.0 14 16189.0 15 17660.0 16 19155.0 17 18725.0 18 18302.0 19 18989.0 20 20881.0 21 22794.0 22 25268.0 23 27780.0 24 30520.0 25 34380.0 26 38225.0 27 42908.0 28 48584.0 29 55024.0 30 63451.0 31 72645.0 32 84279.0 33 98146.0 34 111884.0 35 121542.0 36 109306.0 37 59023.0 38 9638.0 39 82.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.451868832907266 21.826663975973695 14.718975041495094 23.002492149623944 2 14.201580489919591 20.93261357321238 42.183523457923165 22.682282478944867 3 17.504335079598203 27.501660280790357 30.763715048531015 24.230289591080428 4 11.067249976131741 17.356228383826252 42.045325054897 29.531196585145008 5 11.554843518581261 38.81389375590899 37.24028666479515 12.390976060714596 6 27.61065386821687 40.94756972319581 18.6369447907307 12.804831617856621 7 24.433821106128292 34.80809003672415 24.19526067570437 16.562828181443187 8 23.057319393957492 39.35143081304278 20.875875848701124 16.7153739442986 9 24.422076060977695 19.59928802772116 21.59417580106107 34.38446011024007 10-11 22.339660380059293 30.743663619058747 28.65054206382549 18.266133937056473 12-13 22.928248139308533 27.997263169391186 29.142716721970775 19.93177196932951 14-15 20.895454275020555 28.51374886417723 26.850444593483623 23.740352267318592 16-17 20.21149902909744 31.181515906535058 27.821836644893843 20.78514841947366 18-19 20.093854192696416 29.664322856635316 30.01770053876224 20.224122411906023 20-21 20.96594532186738 28.855351304260466 29.998048277710527 20.180655096161626 22-23 20.822668372297855 28.368523097512664 29.796944734117897 21.011863796071584 24-25 20.668914608584707 28.7330825438894 30.003451093817464 20.59455175370843 26-27 20.544338543042116 28.76391075320324 29.96697202396672 20.72477867978792 28-29 20.517179856997966 28.59565895289377 30.25851323026308 20.628647959845186 30-31 20.94782501115331 28.55959240750252 29.877782234935534 20.614800346408636 32-33 20.731052696955537 28.77970081804792 29.809691472308813 20.67955501268773 34-35 20.821714160242898 28.6066171243244 30.0457687732061 20.525899942226598 36-37 20.990078509964814 28.319775726538815 29.954077803296357 20.736067960200014 38-39 20.773606447835324 28.900145892571654 29.993363053025675 20.332884606567344 40-41 20.86468911381648 28.539336136972654 30.106923767534315 20.48905098167655 42-43 20.663788825315272 28.663044430172818 29.783906468939747 20.889260275572163 44-45 20.690457746983977 28.862805009252483 29.607952833842578 20.83878440992096 46-47 20.939159057921525 28.621765188846478 29.517740896898815 20.921334856333182 48-49 20.796604221892455 28.857314184166285 29.39178000724365 20.954301586697614 50-51 21.183784757562652 28.89899260932655 28.8999047566201 21.017317876490697 52-53 20.602463854852644 29.147389661402084 29.103122116727594 21.147024367017682 54-55 20.616980987882854 29.08468982538968 29.193414397355227 21.104914789372234 56-57 20.701025433622387 29.23015486162707 29.061718588746476 21.00710111600407 58-59 20.614847180463325 28.99915641020095 29.301406704015342 21.084589705320383 60-61 20.73965176709402 29.326704080158866 29.074791981676253 20.858852171070858 62-63 20.56896924592004 29.056251709106117 29.243632295090467 21.131146749883378 64-65 20.43697252733576 28.89276710586422 29.265821506738714 21.404438860061305 66-67 20.44222640408224 28.93251181308803 29.039959660302483 21.585302122527246 68-69 20.405450101818428 28.942608017821353 29.134015936953027 21.51792594340719 70-71 20.5938216887571 28.525943334227115 29.399325983291536 21.48090899372425 72-73 20.669213994043016 28.97018675308127 29.060909487540755 21.299689765334957 74-75 20.452230631270965 29.16642315595957 28.82208494036324 21.55926127240622 76-77 20.42791468276256 29.059588590657476 29.104068848065666 21.408427878514296 78-79 20.233736835063894 28.989358683655997 29.09329216347753 21.68361231780258 80-81 20.33507688458095 29.02704403765067 28.85073730645553 21.78714177131285 82-83 20.619238367472096 28.772784387396705 29.057044017066154 21.55093322806504 84-85 20.500983969163546 28.862756631626883 28.86790688344709 21.768352515762484 86-87 20.247883180091367 29.053065928091204 29.14182771158089 21.55722318023654 88-89 20.55993415023459 28.849423831580438 28.94082886382932 21.649813154355652 90-91 20.41785896767547 28.673470339558342 29.31341771767072 21.595252975095462 92-93 20.575600499182595 28.720580367119574 28.861368372368386 21.842450761329445 94-95 20.19348551673592 28.64415466011911 29.016856367047204 22.145503456097764 96-97 20.248995224265002 28.550978926383536 29.028518768211086 22.171507081140373 98-99 20.195023620447916 28.58660491263892 28.710511625483026 22.507859841430143 100-101 20.36587142404369 28.59386743515064 28.690144002041528 22.350117138764148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 112223.0 1 102343.5 2 68854.0 3 35259.0 4 21596.0 5 16758.5 6 15692.5 7 16420.0 8 17136.0 9 16488.0 10 14499.5 11 11892.5 12 9292.0 13 6936.5 14 5323.5 15 4613.5 16 4567.5 17 5055.0 18 5822.0 19 6731.0 20 7702.5 21 8705.0 22 9818.5 23 10982.5 24 12129.5 25 13379.5 26 15258.5 27 17555.0 28 20148.0 29 23699.0 30 28096.5 31 32231.5 32 36491.0 33 41596.0 34 46660.5 35 50618.5 36 55202.5 37 59209.0 38 59797.5 39 58999.0 40 57411.0 41 54912.5 42 52843.5 43 52596.0 44 53094.5 45 53066.5 46 51301.5 47 48781.0 48 47478.0 49 45756.5 50 43183.0 51 40017.5 52 35744.5 53 31472.0 54 27618.5 55 23562.5 56 19173.5 57 15066.5 58 12055.5 59 9377.0 60 7022.5 61 5196.0 62 3595.5 63 2417.5 64 1559.0 65 951.5 66 573.0 67 335.5 68 205.0 69 125.5 70 74.0 71 40.0 72 30.0 73 24.0 74 11.5 75 4.5 76 3.0 77 4.5 78 4.5 79 2.5 80 1.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.436992448951074 2 29.53785977428094 3 23.258498044266414 4 29.656633625185908 5 32.860765417040824 6 25.317244793285838 7 24.23999358740358 8 20.19848719051369 9 11.940211204062466 10-11 15.624069117390594 12-13 15.696833672197169 14-15 19.89107251758319 16-17 23.255708781465863 18-19 27.283404265459826 20-21 29.376055451627685 22-23 28.977109630484964 24-25 27.4950903558666 26-27 28.93773498862089 28-29 25.854272663166455 30-31 27.767029938809536 32-33 26.74865113417383 34-35 24.76857242821846 36-37 27.80329035521668 38-39 27.943268019450095 40-41 24.92417538017923 42-43 25.787194954846438 44-45 24.488427538202068 46-47 27.833484802038168 48-49 23.136907850339533 50-51 28.74776723574849 52-53 22.309038403003523 54-55 23.460245693432398 56-57 25.60973826424447 58-59 24.883257165697376 60-61 24.43916861553993 62-63 24.242674529318673 64-65 22.756403659946077 66-67 23.256467027664073 68-69 24.26623432190584 70-71 22.738909836944572 72-73 22.600421584886206 74-75 19.837914043044552 76-77 22.132637841034768 78-79 21.260763033981345 80-81 22.295173329664866 82-83 23.16813134557287 84-85 20.077817724113203 86-87 21.653209601996572 88-89 20.482152509307472 90-91 21.613239195262476 92-93 21.599157480152904 94-95 20.634316273154944 96-97 19.543010432384477 98-99 18.938579891527464 100-101 20.624107029700504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1846366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.04996062308996 #Duplication Level Percentage of deduplicated Percentage of total 1 84.612619370267 56.73272796992901 2 9.6583435200236 12.951831054037166 3 2.6458348702620844 5.3220937159881325 4 1.0885251680547057 2.9194227862124156 5 0.5231080239410991 1.7537186203436546 6 0.33141571723810265 1.3332846474412736 7 0.21086837473328862 0.9897101355765386 8 0.14692933812338763 0.7881285068439849 9 0.11248432126911412 0.6787862380628191 >10 0.6072310650805005 7.395568593304091 >50 0.04403672621710587 1.9986740018918474 >100 0.01807706497366757 2.0280547547749643 >500 2.632199081881914E-4 0.11377647527611295 >1k 1.7547993879212756E-4 0.16666429909887695 >5k 0.0 0.0 >10k+ 8.773996939606378E-5 4.827558201219146 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 82060 4.444405930351837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.4160442729123046E-5 0.0 5 0.0 0.0 0.0 5.4160442729123046E-5 0.0 6 0.0 0.0 0.0 5.4160442729123046E-5 0.0 7 0.0 0.0 0.0 5.4160442729123046E-5 0.0 8 0.0 0.0 0.0 5.4160442729123046E-5 0.0 9 0.0 0.0 0.0 5.4160442729123046E-5 0.0 10-11 0.0 0.0 0.0 1.3540110682280763E-4 0.0 12-13 0.0 0.0 0.0 2.4372199228105373E-4 0.0 14-15 0.0 0.0 0.0 2.7080221364561525E-4 0.0 16-17 0.0 0.0 0.0 2.7080221364561525E-4 0.0 18-19 0.0 0.0 0.0 2.7080221364561525E-4 0.0 20-21 0.0 0.0 0.0 2.7080221364561525E-4 0.0 22-23 0.0 0.0 0.0 2.9788243501017677E-4 0.0 24-25 0.0 0.0 0.0 3.7912309910386133E-4 0.0 26-27 0.0 0.0 0.0 4.874439845621074E-4 0.0 28-29 0.0 0.0 0.0 7.853264195722842E-4 0.0 30-31 0.0 0.0 0.0 0.0024101397014459755 0.0 32-33 0.0 0.0 0.0 0.00677005534114038 0.0 34-35 0.0 0.0 0.0 0.013188067804541462 0.0 36-37 0.0 0.0 0.0 0.02290986727441905 0.0 38-39 0.0 0.0 0.0 0.04164938045869562 0.0 40-41 0.0 0.0 0.0 0.061607503604377466 0.0 42-43 0.0 0.0 0.0 0.08145730586460107 0.0 44-45 0.0 0.0 0.0 0.10536914132950889 0.0 46-47 0.0 0.0 0.0 0.1347782617314227 0.0 48-49 0.0 0.0 0.0 0.16941386485669688 0.0 50-51 0.0 0.0 0.0 0.20778653853028056 0.0 52-53 0.0 0.0 0.0 0.2528751071022755 0.0 54-55 0.0 0.0 0.0 0.30126746268074694 0.0 56-57 0.0 0.0 2.7080221364561523E-5 0.35946285839318964 0.0 58-59 0.0 0.0 5.4160442729123046E-5 0.428734064643738 0.0 60-61 0.0 0.0 5.4160442729123046E-5 0.5063730592959359 0.0 62-63 0.0 0.0 5.4160442729123046E-5 0.5852035836881745 0.0 64-65 0.0 0.0 5.4160442729123046E-5 0.6659838840186616 0.0 66-67 0.0 0.0 5.4160442729123046E-5 0.7610896214510016 0.0 68-69 0.0 0.0 5.4160442729123046E-5 0.8720372883816101 0.0 70-71 0.0 0.0 5.4160442729123046E-5 0.9950898142621778 0.0 72-73 0.0 0.0 5.4160442729123046E-5 1.1205253996228266 0.0 74-75 0.0 0.0 5.4160442729123046E-5 1.2444174123656957 0.0 76-77 0.0 0.0 5.4160442729123046E-5 1.3845846381486662 0.0 78-79 0.0 0.0 5.4160442729123046E-5 1.5462806399164628 0.0 80-81 0.0 0.0 5.4160442729123046E-5 1.7270140373035465 0.0 82-83 0.0 0.0 5.4160442729123046E-5 1.91516741534452 0.0 84-85 0.0 0.0 5.4160442729123046E-5 2.0997462041653714 0.0 86-87 0.0 0.0 5.4160442729123046E-5 2.300302323591314 0.0 88-89 0.0 0.0 5.4160442729123046E-5 2.521087368376584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1490 0.0 55.095963 1 GTATCAA 2695 0.0 44.54953 1 TCAACGC 3375 0.0 36.008354 4 CAACGCA 3380 0.0 35.79711 5 ATCAACG 3325 0.0 35.18778 3 TATCAAC 3475 0.0 34.395115 2 AACGCAG 3740 0.0 31.493584 6 GTACATG 3600 0.0 28.076939 1 CGCAGAG 4230 0.0 27.21659 8 ACGCAGA 4240 0.0 27.078869 7 ACATGGG 3625 0.0 25.458841 3 TACATGG 3760 0.0 25.23943 2 GAGTACA 2400 0.0 25.012705 1 GTGATCG 160 1.2323471E-7 24.907106 8 GTGTACG 125 1.3374665E-4 24.627893 1 GTGGTAT 805 0.0 24.219978 1 GCAGAGT 4625 0.0 23.022116 9 GCGTGCG 270 5.456968E-12 22.62723 9 AGTACAT 2555 0.0 21.28191 2 TTCGCAC 145 4.1050132E-4 20.867903 3 >>END_MODULE