FastQCFastQC Report
Sat 18 Jun 2016
SRR1931030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846366
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45050.24399279449469932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38940.21090076398720511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.1304725065344574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.059.215231
GTATCAA48250.039.5101741
CAACGCA59050.031.333715
ATCAACG59150.031.264033
TCAACGC60550.030.5411644
AACGCAG63250.029.3272676
TATCAAC63200.029.2605612
ACGCAGA74800.024.608527
CGCAGAG74750.024.3076488
GTACATG57700.023.0698171
GGACCGT1252.2756387E-422.7717576
TCTAACG1050.002186265822.6376232
CTGTCCG2154.4849003E-822.0650629
ACATGGG56300.021.8696333
TACATGG59950.021.8068852
GCAGAGT82700.021.6835259
TAACGCC1100.00286434221.608644
CTAACGC1553.883407E-521.4692293
GTATACG4400.020.5287651
GGTGCGT1404.894E-420.3319287
Produced by FastQC (version 0.11.5)