##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931030_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1846366 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30982047979653 31.0 31.0 34.0 30.0 34.0 2 31.4715218976086 31.0 31.0 34.0 30.0 34.0 3 31.53329079933231 31.0 31.0 34.0 30.0 34.0 4 35.3182343045745 37.0 35.0 37.0 33.0 37.0 5 35.19763524674956 37.0 35.0 37.0 33.0 37.0 6 35.21887426436579 37.0 35.0 37.0 32.0 37.0 7 35.092347887688575 37.0 35.0 37.0 32.0 37.0 8 35.09310667549121 37.0 35.0 37.0 32.0 37.0 9 36.586324704852665 39.0 35.0 39.0 32.0 39.0 10-11 36.520859352912694 38.0 35.0 39.0 32.0 39.0 12-13 36.41320139127345 38.0 35.0 39.0 32.0 39.0 14-15 37.46573810392955 39.0 36.0 41.0 32.0 41.0 16-17 37.41688348897239 39.0 36.0 41.0 32.0 41.0 18-19 37.44677490811681 39.0 36.0 41.0 32.0 41.0 20-21 37.428308092761675 39.0 36.0 41.0 32.0 41.0 22-23 37.32889416291245 39.0 36.0 40.5 32.0 41.0 24-25 37.21355191765879 39.0 36.0 40.0 31.0 41.0 26-27 36.960261941565214 39.0 36.0 40.0 31.0 41.0 28-29 36.77736862572209 39.0 35.5 40.0 30.0 41.0 30-31 36.557968192655196 38.0 35.0 40.0 30.0 41.0 32-33 36.40294042459621 38.0 35.0 40.0 30.0 41.0 34-35 36.49847077989954 38.0 35.0 40.0 30.0 41.0 36-37 36.57882889957896 38.0 35.0 40.0 30.0 41.0 38-39 36.56418716549157 38.5 35.0 40.0 30.0 41.0 40-41 36.463163045680005 39.0 35.0 40.0 30.0 41.0 42-43 36.33749402881119 38.0 35.0 40.0 30.0 41.0 44-45 36.17515730900591 38.0 35.0 40.0 30.0 41.0 46-47 35.945360508154934 38.0 35.0 40.0 29.0 41.0 48-49 35.73262722558799 38.0 35.0 40.0 28.0 41.0 50-51 35.48206341537918 38.0 34.5 40.0 27.5 41.0 52-53 35.290539903789394 38.0 34.0 40.0 27.0 41.0 54-55 35.15042873406465 38.0 34.0 40.0 27.0 41.0 56-57 34.97771676904796 38.0 34.0 40.0 26.0 41.0 58-59 34.78407883377402 38.0 33.5 40.0 26.0 41.0 60-61 34.48711089783932 37.5 33.0 40.0 26.0 41.0 62-63 34.22966356616186 37.0 33.0 40.0 26.0 41.0 64-65 33.92144136103026 37.0 32.5 39.5 25.0 41.0 66-67 33.610038042294974 36.0 32.0 39.0 24.5 41.0 68-69 33.27328140791154 36.0 32.0 39.0 23.5 40.0 70-71 32.91471328003224 35.5 31.5 39.0 23.0 40.0 72-73 32.49811034215318 35.0 31.0 38.0 22.0 40.0 74-75 31.97563457082724 35.0 31.0 37.5 21.0 39.5 76-77 30.585756561808438 33.5 29.5 36.0 20.0 38.5 78-79 31.332918825411646 34.0 30.0 36.0 21.0 39.0 80-81 31.305343848402757 34.0 31.0 36.0 21.0 38.5 82-83 31.032463498569623 34.0 30.5 36.0 20.0 37.0 84-85 30.68183854122097 34.0 30.0 35.0 20.0 37.0 86-87 30.349123359073985 34.0 30.0 35.0 18.5 36.5 88-89 29.99931351638841 34.0 30.0 35.0 18.0 36.0 90-91 29.658644602424438 34.0 29.0 35.0 13.0 36.0 92-93 29.333754521042955 34.0 29.0 35.0 7.0 35.5 94-95 28.951601415970615 34.0 29.0 35.0 2.0 35.0 96-97 28.527903460094045 34.0 29.0 35.0 2.0 35.0 98-99 28.035842026987062 33.5 29.0 35.0 2.0 35.0 100-101 26.89383009652474 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24733925874506824 1101 2 0.4193127385243791 1101 3 0.22094536148220456 1101 4 0.1985858670418139 1101 5 0.20626003348480282 1101 6 0.30139110414222614 1101 7 0.4072781744728573 1101 8 0.4146709200665981 1101 9 0.485811538628397 1101 10-11 0.5296609121559115 1101 12-13 0.38850295837662685 1101 14-15 0.5270399552500606 1101 16-17 0.604526414527804 1101 18-19 0.4932580646338778 1101 20-21 0.526894408829925 1101 22-23 0.5704692771274367 1101 24-25 0.5456582236985099 1101 26-27 0.49407753352948447 1101 28-29 0.5844440458330382 1101 30-31 0.5882593419385458 1101 32-33 0.3812832489497353 1101 34-35 0.46744808482081623 1101 36-37 0.4706968751275582 1101 38-39 0.5958755044735753 1101 40-41 0.5358799735496262 1101 42-43 0.48597225062503924 1101 44-45 0.47492803332102085 1101 46-47 0.4005535071591382 1101 48-49 0.500164718055423 1101 50-51 0.45369524192584265 1101 52-53 0.3932721328833395 1101 54-55 0.29069492360782334 1101 56-57 0.259700810882066 1101 58-59 0.3717162510501666 1101 60-61 0.3182629230604661 1101 62-63 0.31172891916221346 1101 64-65 0.3919279442666195 1101 66-67 0.2592827931464612 1101 68-69 0.27856678841269655 1101 70-71 0.11057473687144892 1101 72-73 0.21976157728016688 1101 74-75 0.37859292711847203 1101 76-77 0.312986161619353 1101 78-79 0.4091464398305753 1101 80-81 0.40024275590868896 1101 82-83 0.3843945596279461 1101 84-85 0.26884247545723383 1101 86-87 0.26485303591988085 1101 88-89 0.3305296174531591 1101 90-91 0.2308179422322567 1101 92-93 0.12095542874542176 1101 94-95 0.2659147400367061 1101 96-97 0.5298195723225625 1101 98-99 0.5490638018795337 1101 100-101 0.6890310061130336 1102 1 0.3638624110652202 1102 2 0.4681155828498582 1102 3 0.3887546600871765 1102 4 0.37008890860872157 1102 5 0.4071212601493741 1102 6 0.4018897608479435 1102 7 0.4606601691277987 1102 8 0.45071760007013495 1102 9 0.5003267085048648 1102 10-11 0.47205671371107627 1102 12-13 0.45265323863378626 1102 14-15 0.6017008279203822 1102 16-17 0.6271954017921644 1102 18-19 0.6709980405375262 1102 20-21 0.5018679436128792 1102 22-23 0.6649230392652328 1102 24-25 0.6807608721884151 1102 26-27 0.6851562599937253 1102 28-29 0.6317138054740354 1102 30-31 0.5528424848956988 1102 32-33 0.5633088326958031 1102 34-35 0.5778567527593097 1102 36-37 0.645400626369991 1102 38-39 0.5495104124111805 1102 40-41 0.7086443480637286 1102 42-43 0.6077268699125256 1102 44-45 0.6893009306894058 1102 46-47 0.4896983397288608 1102 48-49 0.5994461856553883 1102 50-51 0.4047052783234193 1102 52-53 0.4022909727438275 1102 54-55 0.3130991884090193 1102 56-57 0.30928629458750123 1102 58-59 0.36462202644236896 1102 60-61 0.28100158448986434 1102 62-63 0.1960408446069195 1102 64-65 0.1578278108509963 1102 66-67 -0.0907969891147502 1102 68-69 0.07522043217751673 1102 70-71 0.24679996043751373 1102 72-73 0.22986587764948752 1102 74-75 0.36794540444554613 1102 76-77 0.33417618960992357 1102 78-79 0.30923528985538695 1102 80-81 0.271532951055395 1102 82-83 0.2507047952351549 1102 84-85 0.5208713348450402 1102 86-87 0.4805061046900825 1102 88-89 0.6333348601669684 1102 90-91 0.6077326813548076 1102 92-93 0.4284178467313673 1102 94-95 0.6989547183303095 1102 96-97 0.6602601250484419 1102 98-99 0.7522945968482588 1102 100-101 1.0095678739528395 1103 1 0.3897013420760409 1103 2 0.39989274521262175 1103 3 0.3858921672794402 1103 4 0.3288904088121569 1103 5 0.3516610163164273 1103 6 0.3972027337496087 1103 7 0.3866724350535975 1103 8 0.4106188651323137 1103 9 0.5315350307980538 1103 10-11 0.5794132204499007 1103 12-13 0.5674145885508111 1103 14-15 0.5513870013705997 1103 16-17 0.7072358613874457 1103 18-19 0.5923048483259024 1103 20-21 0.5842211521657674 1103 22-23 0.4517026627953271 1103 24-25 0.5883857514858946 1103 26-27 0.5696772081861496 1103 28-29 0.6710036533805521 1103 30-31 0.6881211843794119 1103 32-33 0.7922280199340932 1103 34-35 0.6607976758744414 1103 36-37 0.5770069205282624 1103 38-39 0.6869822669672772 1103 40-41 0.6856140025357931 1103 42-43 0.7509101293184486 1103 44-45 0.7873820516513419 1103 46-47 0.7268330587067879 1103 48-49 0.6888149682977271 1103 50-51 0.6347842352319333 1103 52-53 0.6584031487594189 1103 54-55 0.5918322082945835 1103 56-57 0.5996863007958595 1103 58-59 0.655249995502615 1103 60-61 0.45589066566514447 1103 62-63 0.3744911351244369 1103 64-65 0.4097148443794083 1103 66-67 0.4536568756385648 1103 68-69 0.4436402740641441 1103 70-71 0.5940213357866 1103 72-73 0.559988098997124 1103 74-75 0.7089975091316312 1103 76-77 0.6267578488136394 1103 78-79 0.6100949800365498 1103 80-81 0.45964526343296086 1103 82-83 0.4753596220609957 1103 84-85 0.52465912124676 1103 86-87 0.6872048034864378 1103 88-89 0.530905122613877 1103 90-91 0.7676643507025176 1103 92-93 0.5451089125831139 1103 94-95 0.620519283507452 1103 96-97 0.7804519796932325 1103 98-99 0.7687312034971789 1103 100-101 1.0506805931990577 1104 1 0.29877131884115826 1104 2 0.35965392940937946 1104 3 0.38621827088122984 1104 4 0.07564286863586034 1104 5 0.19333523881823567 1104 6 0.30741049259158615 1104 7 0.2843119848102873 1104 8 0.3550914641670033 1104 9 0.3941063246471401 1104 10-11 0.4216685904177453 1104 12-13 0.4304527283500974 1104 14-15 0.4095981743850814 1104 16-17 0.5191447297528597 1104 18-19 0.5340569335711436 1104 20-21 0.4286267677579261 1104 22-23 0.3519112424048174 1104 24-25 0.3847311602005732 1104 26-27 0.35263364030426914 1104 28-29 0.5860290145458009 1104 30-31 0.4409832667932818 1104 32-33 0.49984240132327074 1104 34-35 0.5449024113173877 1104 36-37 0.5719356514674558 1104 38-39 0.6176305796565629 1104 40-41 0.5335837597318331 1104 42-43 0.46983099631334824 1104 44-45 0.5451865040672104 1104 46-47 0.3912475264693498 1104 48-49 0.4456324340100224 1104 50-51 0.39832304321309664 1104 52-53 0.2646925414685768 1104 54-55 0.48975875125488244 1104 56-57 0.44573785821597767 1104 58-59 0.48604230055931197 1104 60-61 0.2603074139495192 1104 62-63 0.29062012562446427 1104 64-65 0.43143061754791745 1104 66-67 0.4195285087015037 1104 68-69 0.2788992895617781 1104 70-71 0.36923129252278386 1104 72-73 0.37162044032183417 1104 74-75 0.5499066000407247 1104 76-77 0.6143473207351242 1104 78-79 0.8340496582461157 1104 80-81 0.640096371579272 1104 82-83 0.657563812492274 1104 84-85 0.8438868671547652 1104 86-87 0.8386826807723295 1104 88-89 0.9512804832833588 1104 90-91 0.7823691718578303 1104 92-93 0.7567015375738855 1104 94-95 1.0130870133526493 1104 96-97 0.8309623703895852 1104 98-99 0.9121286521771736 1104 100-101 1.1582764660054323 1105 1 0.29559772959283137 1105 2 0.3054451614552107 1105 3 0.28345097256517704 1105 4 0.24882547915982656 1105 5 0.21366899666649175 1105 6 0.28565563701871355 1105 7 0.2734712451457355 1105 8 0.2991857038116805 1105 9 0.5304300467598324 1105 10-11 0.399179038527798 1105 12-13 0.33988963371902514 1105 14-15 0.5448792867796115 1105 16-17 0.4375698247484152 1105 18-19 0.5378977129922831 1105 20-21 0.41079900875112685 1105 22-23 0.45743014986189934 1105 24-25 0.3704380015613964 1105 26-27 0.37682285282242134 1105 28-29 0.49582119434587923 1105 30-31 0.31426676298185185 1105 32-33 0.42414533367811913 1105 34-35 0.40999485070927477 1105 36-37 0.4815481261759942 1105 38-39 0.4269314588106923 1105 40-41 0.40529486972229023 1105 42-43 0.5554303881710396 1105 44-45 0.587638663423327 1105 46-47 0.35995576037787913 1105 48-49 0.38973654609477393 1105 50-51 0.5008229851791555 1105 52-53 0.4105533075112291 1105 54-55 0.41394601596728364 1105 56-57 0.5511844567201152 1105 58-59 0.6607157829451467 1105 60-61 0.5936506197229221 1105 62-63 0.48623078540497744 1105 64-65 0.30926210673612786 1105 66-67 0.4080284501339264 1105 68-69 0.17201798537509205 1105 70-71 0.19587417995001744 1105 72-73 0.2088828713915234 1105 74-75 0.24908734760856888 1105 76-77 0.15184244788548185 1105 78-79 0.30575617888604967 1105 80-81 0.2685703031344424 1105 82-83 0.3070741794882288 1105 84-85 0.3356807348752753 1105 86-87 0.27414320475341825 1105 88-89 0.3318897302376449 1105 90-91 0.3942091091865123 1105 92-93 0.32954920555679124 1105 94-95 0.3164967140122634 1105 96-97 0.31368093087042226 1105 98-99 0.3037193877726878 1105 100-101 0.594953701519426 1106 1 0.2592434029159243 1106 2 0.27011490031850016 1106 3 0.239928187106905 1106 4 0.042476272810624494 1106 5 0.07183591934954592 1106 6 0.26722245729521177 1106 7 0.3031691458113741 1106 8 0.24439819741127167 1106 9 0.37374801911006017 1106 10-11 0.20691073335627408 1106 12-13 0.2607805707389943 1106 14-15 0.30908819614981553 1106 16-17 0.2734633219322333 1106 18-19 0.40681732384737757 1106 20-21 0.33092189661594773 1106 22-23 0.18426066701499622 1106 24-25 0.3968076892214114 1106 26-27 0.4351945886135056 1106 28-29 0.47687598185227387 1106 30-31 0.3723824823878985 1106 32-33 0.4733004796074525 1106 34-35 0.5482436986355381 1106 36-37 0.3596843268928396 1106 38-39 0.3227327900958912 1106 40-41 0.44856163879550337 1106 42-43 0.46556863701896845 1106 44-45 0.3572239168795548 1106 46-47 0.45416979007577396 1106 48-49 0.3879444313994327 1106 50-51 0.37332682542493956 1106 52-53 0.34014880162285266 1106 54-55 0.6610018275392235 1106 56-57 0.682008829479976 1106 58-59 0.6886215689154511 1106 60-61 0.622068438463323 1106 62-63 0.39611301786785447 1106 64-65 0.4982021989020211 1106 66-67 0.32661523968845074 1106 68-69 0.1405279700141122 1106 70-71 0.32593050355472286 1106 72-73 0.5230149758462019 1106 74-75 0.6302957449460003 1106 76-77 0.618816334214209 1106 78-79 0.5013526970632114 1106 80-81 0.4554622642199533 1106 82-83 0.5602743843013371 1106 84-85 0.4682972223304702 1106 86-87 0.39651903680666933 1106 88-89 0.43250416956175997 1106 90-91 0.5194011214752976 1106 92-93 0.5707166402726109 1106 94-95 0.7179304057685343 1106 96-97 0.8280178484331451 1106 98-99 0.9227670068203047 1106 100-101 1.2605963026458973 1107 1 0.2228753841417337 1107 2 0.316949106587618 1107 3 0.18041923221694134 1107 4 0.054114038818362076 1107 5 0.09514038560833882 1107 6 0.20327183771489388 1107 7 0.27495076667668883 1107 8 0.15673880719011635 1107 9 0.3231164517472678 1107 10-11 0.17887177236428187 1107 12-13 0.2109063313608317 1107 14-15 0.3009787286070633 1107 16-17 0.29713347052507544 1107 18-19 0.32359016885335024 1107 20-21 0.34114072216467406 1107 22-23 0.36500616812767817 1107 24-25 0.25889843114190825 1107 26-27 0.37281106831846245 1107 28-29 0.3763243195025012 1107 30-31 0.42456978201180107 1107 32-33 0.5380436907814428 1107 34-35 0.33031834071636723 1107 36-37 0.3542827945146456 1107 38-39 0.4334742145803645 1107 40-41 0.562764967990212 1107 42-43 0.49899143399926515 1107 44-45 0.5377633906793378 1107 46-47 0.5069564418826431 1107 48-49 0.5752642059396678 1107 50-51 0.42789047895183785 1107 52-53 0.4783145927703103 1107 54-55 0.34069032457578885 1107 56-57 0.49082814184735213 1107 58-59 0.39926350610436856 1107 60-61 0.37588013750792726 1107 62-63 0.3533870866373192 1107 64-65 0.20583419976178874 1107 66-67 0.3294686210939659 1107 68-69 0.37378163084174076 1107 70-71 0.2613235674999217 1107 72-73 0.2785761632455461 1107 74-75 0.5282113427248838 1107 76-77 0.5252344660644539 1107 78-79 0.5427707746617507 1107 80-81 0.5005730746216095 1107 82-83 0.5644108185588586 1107 84-85 0.52423538158558 1107 86-87 0.5224487431292708 1107 88-89 0.5182921342599158 1107 90-91 0.5663837449121409 1107 92-93 0.4660578654208116 1107 94-95 0.6684295989681637 1107 96-97 0.694309093292798 1107 98-99 0.745235919300935 1107 100-101 0.7979501524244732 1108 1 0.0554999149341171 1108 2 0.0778311066112174 1108 3 0.11659571019849224 1108 4 0.09291058957486342 1108 5 0.08262869549646723 1108 6 0.14844360989737737 1108 7 0.14695359641478944 1108 8 0.1474472523372654 1108 9 0.14385259405909068 1108 10-11 0.08111922362491697 1108 12-13 0.18167515212031304 1108 14-15 0.10209484893146481 1108 16-17 0.2534047570452955 1108 18-19 0.24829853807569435 1108 20-21 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68-69 -0.9538654275788119 2316 70-71 -0.9781064212035702 2316 72-73 -1.7942753592484806 2316 74-75 -2.767812340896139 2316 76-77 -1.6271502345301059 2316 78-79 -1.3574623485661022 2316 80-81 -1.0470103013562202 2316 82-83 -0.9464498260888625 2316 84-85 -0.9606965550635422 2316 86-87 -0.886201786450254 2316 88-89 -0.760574419647174 2316 90-91 -0.8726532979097783 2316 92-93 -0.7892648180926578 2316 94-95 -1.0710625582129332 2316 96-97 -1.063415040338949 2316 98-99 -1.1726405493651235 2316 100-101 -1.6395244578322625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 23.0 5 46.0 6 80.0 7 222.0 8 536.0 9 1066.0 10 1844.0 11 2940.0 12 4287.0 13 5551.0 14 6896.0 15 9100.0 16 11081.0 17 13165.0 18 11105.0 19 8650.0 20 8538.0 21 8935.0 22 10029.0 23 12080.0 24 14762.0 25 18927.0 26 24687.0 27 31758.0 28 41179.0 29 51512.0 30 63296.0 31 77283.0 32 92946.0 33 113164.0 34 140437.0 35 174223.0 36 226707.0 37 308494.0 38 293987.0 39 56809.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.40832376454276 23.000790779296747 15.081840240916872 22.509045215243624 2 14.842665438936208 22.49552465165405 41.053609392138014 21.60820051727173 3 17.702918323553593 27.863755961814057 31.318818805264918 23.114506909367428 4 11.950511964834936 18.6878422389842 40.53552720740584 28.826118588775024 5 11.550806193908999 39.51195073470073 36.67621714428081 12.261025927109454 6 27.9799778590911 41.286187495802544 18.174772362426314 12.559062282680047 7 24.953232457703404 33.842044318407076 24.203381128118696 17.001342095770827 8 23.671813392029257 38.32454483218801 20.983864398554555 17.019777377228177 9 24.765891486303364 16.737743221008188 22.16061170970436 36.33575358298409 10-11 23.153074742494177 28.222790064375104 29.718186968347553 18.90594822478317 12-13 23.686798825368317 25.6071927234362 30.103836400800276 20.60217205039521 14-15 21.235713829219126 27.299192034515368 27.36849032098728 24.09660381527823 16-17 20.182780662122244 30.65112767457806 28.293577763022064 20.872513900277625 18-19 20.051739470939133 29.37402443502534 30.574382327230897 19.99985376680463 20-21 20.778545532142598 28.679822960344808 30.498855589845135 20.04277591766746 22-23 20.69066648107643 28.27574665091316 30.43339562702104 20.600191240989375 24-25 20.49409823200162 28.74655367999682 30.461095342793243 20.298252745208327 26-27 20.382832106161533 28.792648722308744 30.491461792751355 20.333057378778363 28-29 20.34005800055948 28.60967367441316 30.68583806583922 20.364430259188136 30-31 20.871638269603558 28.57911593118842 30.190888710630524 20.3583570885775 32-33 20.629015482291248 28.799607796400828 30.187244715544047 20.384132005763874 34-35 20.68407031806063 28.642600775895037 30.513653997614465 20.15967490842987 36-37 20.84864848031045 28.432080093265217 30.47985279044167 20.239418635982666 38-39 20.562707316749528 28.92554239843324 30.51323428617233 19.9985159986449 40-41 20.64495809811535 28.576635792924044 30.595227198501686 20.183178910458924 42-43 20.451861142384658 28.748223682125833 30.233356778336923 20.566558397152583 44-45 20.485970435110417 28.868665246847115 30.185365531316556 20.459998786725915 46-47 20.799107348396827 28.642660405105662 29.949680015816398 20.608552230681106 48-49 20.63698279915155 28.888200984948448 29.840975025295364 20.63384119060464 50-51 20.842846449020147 28.884301792250643 29.41652076497795 20.856330993751257 52-53 20.671436720158066 29.13802863333413 29.379802676811163 20.81073196969664 54-55 20.735975818672543 29.072791808466985 29.456935813038903 20.734296559821566 56-57 20.711550440081243 29.293324306025724 29.227027758970888 20.768097494922138 58-59 20.569002702791277 29.064878637114994 29.62737557314803 20.7387430869457 60-61 20.81617292593374 29.331675450445445 29.36841386049382 20.483737763127 62-63 20.644722609400215 29.083525942131853 29.61633413091246 20.65541731755547 64-65 20.388317098032083 28.86693115481243 29.590441508803984 21.154310238351506 66-67 20.492990248246205 28.92975045066568 29.406750211758574 21.17050908932954 68-69 20.45566465687379 29.08348558351409 29.38579607615314 21.07505368345898 70-71 20.68302279809876 28.609441107150875 29.589131524653222 21.118404570097145 72-73 20.738851962172912 29.02075520724558 29.356540296109284 20.88385253447222 74-75 20.56643872035055 29.237088609038665 29.200916999190213 20.995555671420572 76-77 20.3981521208465 29.164777184993895 29.410050224254675 21.027020469904933 78-79 20.366197297527858 29.118697267061066 29.386940443515247 21.128164991895833 80-81 20.446742653808972 29.20595331908913 29.110333005662476 21.236971021439423 82-83 20.692999112851947 29.026422713589827 29.300284992249644 20.980293181308582 84-85 20.533875732113785 29.04862849510877 29.09271509549028 21.32478067728717 86-87 20.225838214091898 29.214467770745344 29.368066786325137 21.19162722883762 88-89 20.50685522449374 29.04809475696984 29.152272396770844 21.29277762176557 90-91 20.425212118586003 28.825595597574548 29.501829378931948 21.2473629049075 92-93 20.66152972349725 28.955366339669332 29.143796760578528 21.239307176254897 94-95 20.227371819001633 28.782954154626918 29.23301278492565 21.756661241445798 96-97 20.305735864793796 28.788511480666067 29.244596815905847 21.66115583863429 98-99 20.273201521258517 28.8908862056602 29.12448019515091 21.71143207793038 100-101 20.261042501865827 28.82020682789869 29.05041578971883 21.868334880516645 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 173.0 1 118.0 2 94.5 3 134.0 4 126.5 5 136.5 6 226.0 7 492.0 8 887.0 9 1112.0 10 1084.0 11 979.5 12 991.5 13 1117.0 14 1286.5 15 1474.5 16 1691.5 17 1926.0 18 2150.5 19 2424.5 20 2757.0 21 3191.0 22 3762.5 23 4702.0 24 5928.5 25 7563.0 26 10479.5 27 14364.5 28 18413.5 29 23901.5 30 31064.5 31 37826.0 32 44877.5 33 53083.5 34 60910.5 35 66717.0 36 74543.5 37 82338.0 38 83474.5 39 81833.5 40 78924.5 41 74945.0 42 71765.0 43 71571.5 44 73666.5 45 75216.5 46 72746.0 47 68221.0 48 66840.0 49 65916.0 50 63304.5 51 58214.5 52 51194.0 53 45104.0 54 39868.0 55 34056.0 56 27490.0 57 21238.0 58 16595.5 59 12823.5 60 9350.0 61 6401.5 62 4178.0 63 2719.5 64 1707.5 65 929.5 66 488.5 67 259.0 68 147.5 69 88.5 70 51.0 71 29.5 72 21.0 73 8.5 74 4.0 75 3.5 76 3.0 77 2.0 78 1.5 79 0.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004657798074704581 2 0.00763662242480635 3 0.0017331341673319375 4 0.17185108477950742 5 0.0019497759382484296 6 5.416044272912304E-4 7 0.0 8 7.582461982077226E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.437219922810537E-4 24-25 6.770055341140381E-4 26-27 0.0025726210296333446 28-29 2.437219922810537E-4 30-31 0.005795167372016166 32-33 0.020905930893441498 34-35 0.01299850625498953 36-37 0.0010019681904887764 38-39 3.5204287773929976E-4 40-41 0.0015706528391445686 42-43 0.010913329209918294 44-45 0.006607574012953012 46-47 0.00980304013397127 48-49 0.009640558805783902 50-51 0.19040103641423206 52-53 0.11276204176203417 54-55 0.016979298795580074 56-57 0.006282611356578274 58-59 0.016870977910121827 60-61 0.1216985148123395 62-63 0.2347042785666547 64-65 0.24066192726685826 66-67 0.25162941691950563 68-69 0.246484174860239 70-71 0.23313362572751017 72-73 0.1584192949826849 74-75 0.2785471569558798 76-77 0.212823459704089 78-79 0.005064001395173005 80-81 5.4160442729123046E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.7080221364561523E-5 90-91 0.006011809142932659 92-93 0.017683384551058674 94-95 0.0027621825791852754 96-97 5.4160442729123046E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1846366.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.32173037229465 #Duplication Level Percentage of deduplicated Percentage of total 1 71.79603147086688 33.97512443065144 2 15.554710545518125 14.721516369081938 3 5.296128362061288 7.5186587509958045 4 2.378377852975892 4.501958219278489 5 1.2849654724101778 3.040339481155133 6 0.791252067542476 2.2466050198059433 7 0.5054080646268242 1.6741748913577694 8 0.3638038313922043 1.377266145403971 9 0.2718068691640385 1.1576134238326379 >10 1.4858123441203848 13.333127562901472 >50 0.16402377980348656 5.3917480374346605 >100 0.10308297203134999 8.983414995968205 >500 0.0036770939895347547 1.1421348383295726 >1k 9.192734973836887E-4 0.936317833802938 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4505 0.24399279449469932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3894 0.21090076398720511 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.1304725065344574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.4160442729123046E-5 0.0 10-11 0.0 0.0 0.0 8.124066409368457E-5 0.0 12-13 0.0 0.0 0.0 1.0832088545824609E-4 0.0 14-15 0.0 0.0 0.0 1.6248132818736915E-4 0.0 16-17 0.0 0.0 0.0 1.6248132818736915E-4 0.0 18-19 0.0 0.0 0.0 1.6248132818736915E-4 0.0 20-21 0.0 0.0 0.0 1.6248132818736915E-4 0.0 22-23 0.0 0.0 0.0 1.6248132818736915E-4 0.0 24-25 0.0 0.0 0.0 2.1664177091649218E-4 0.0 26-27 0.0 0.0 0.0 3.249626563747383E-4 0.0 28-29 0.0 0.0 0.0 7.582461982077227E-4 0.0 30-31 0.0 0.0 0.0 0.0034391881132993136 0.0 32-33 0.0 0.0 0.0 0.01083208854582461 0.0 34-35 0.0 0.0 0.0 0.022774466167596243 0.0 36-37 0.0 0.0 0.0 0.04043077049729035 0.0 38-39 0.0 0.0 0.0 0.0721417097151919 0.0 40-41 0.0 0.0 0.0 0.1121121164492847 0.0 42-43 0.0 0.0 0.0 0.1509722341074305 0.0 44-45 0.0 0.0 0.0 0.19833554127404857 0.0 46-47 0.0 0.0 0.0 0.25856195358883344 0.0 48-49 0.0 0.0 0.0 0.3260458652293208 0.0 50-51 0.0 0.0 0.0 0.3964002803344515 0.0 52-53 0.0 0.0 0.0 0.46932731646921577 0.0 54-55 0.0 0.0 0.0 0.5507304618910878 0.0 56-57 0.0 0.0 0.0 0.6486796225667066 0.0 58-59 0.0 0.0 0.0 0.7622811511910423 0.0 60-61 0.0 0.0 0.0 0.8909663631154386 0.0 62-63 0.0 0.0 0.0 1.0219533938558227 0.0 64-65 0.0 0.0 0.0 1.1522634190620926 0.0 66-67 0.0 0.0 0.0 1.3083808952287899 0.0 68-69 0.0 0.0 0.0 1.4875707199980934 0.0 70-71 0.0 0.0 0.0 1.6879914383172134 0.0 72-73 0.0 0.0 0.0 1.8935032382528707 0.0 74-75 0.0 0.0 0.0 2.1006669316917668 0.0 76-77 0.0 0.0 0.0 2.3339630387474637 0.0 78-79 0.0 0.0 0.0 2.602355112691633 0.0 80-81 0.0 0.0 0.0 2.900102146594987 0.0 82-83 0.0 0.0 0.0 3.212499580256569 0.0 84-85 0.0 0.0 0.0 3.5229201577585374 0.0 86-87 0.0 0.0 0.0 3.8537050617266564 0.0 88-89 0.0 0.0 0.0 4.2170945522177075 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2545 0.0 59.21523 1 GTATCAA 4825 0.0 39.510174 1 CAACGCA 5905 0.0 31.33371 5 ATCAACG 5915 0.0 31.26403 3 TCAACGC 6055 0.0 30.541164 4 AACGCAG 6325 0.0 29.327267 6 TATCAAC 6320 0.0 29.260561 2 ACGCAGA 7480 0.0 24.60852 7 CGCAGAG 7475 0.0 24.307648 8 GTACATG 5770 0.0 23.069817 1 GGACCGT 125 2.2756387E-4 22.771757 6 TCTAACG 105 0.0021862658 22.637623 2 CTGTCCG 215 4.4849003E-8 22.065062 9 ACATGGG 5630 0.0 21.869633 3 TACATGG 5995 0.0 21.806885 2 GCAGAGT 8270 0.0 21.683525 9 TAACGCC 110 0.002864342 21.60864 4 CTAACGC 155 3.883407E-5 21.469229 3 GTATACG 440 0.0 20.528765 1 GGTGCGT 140 4.894E-4 20.331928 7 >>END_MODULE