FastQCFastQC Report
Sat 18 Jun 2016
SRR1931029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529750
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN653294.270567086125184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.045.778841
GTATCAA19850.040.702661
ATCAACG21200.035.5905653
TCAACGC20900.035.133634
CAACGCA21900.033.9771425
TATCAAC24200.032.115282
AACGCAG24200.030.3849546
ACGCAGA27750.026.415397
CGCAGAG27350.025.8544948
GTACATG21850.022.2330171
TACATGG21750.021.1576162
ACATGGG22400.020.2103583
GCAGAGT31700.020.0802849
CTAATAC3907.2759576E-1219.3466683
GAGTACT21550.018.8247912-13
AGAGTAC29900.018.64461110-11
AGTACTT23850.018.41846312-13
TATACTG8450.017.2548245
GTACTTT24350.017.1959214-15
GTGGTAT6000.017.0453111
Produced by FastQC (version 0.11.5)