FastQCFastQC Report
Sat 18 Jun 2016
SRR1931029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529750
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.17669553848668082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22890.1496322928583102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.052.5570371
GTATCAA34800.041.1195681
TCAACGC41700.034.0831344
CAACGCA42400.033.2243925
ATCAACG42350.033.1121373
AACGCAG44500.032.1883856
TATCAAC44250.031.6924482
ACGCAGA52100.027.2198497
CGCAGAG52100.027.128818
GTGGTAT10150.022.9504591
TAGACCG1252.2785465E-422.7670975
GCAGAGT61350.022.2646569
TGGTATC10250.020.8706362
GAGTACT41850.020.8526112-13
TAACGCC1150.003708422420.6669584
GCGTGCA2551.2134478E-820.459299
AGAGTAC54900.019.56743610-11
CCTATAC3900.019.5017683
CAGAGTA58650.019.4888910-11
GTACTTT44550.019.26943214-15
Produced by FastQC (version 0.11.5)