##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931029_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1529750 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.34932701421801 31.0 31.0 34.0 30.0 34.0 2 31.51580715803236 31.0 31.0 34.0 30.0 34.0 3 31.585812714495834 31.0 31.0 34.0 30.0 34.0 4 35.358671024677236 37.0 35.0 37.0 33.0 37.0 5 35.2370753391077 37.0 35.0 37.0 33.0 37.0 6 35.2576636705344 37.0 35.0 37.0 32.0 37.0 7 35.14367837882007 37.0 35.0 37.0 32.0 37.0 8 35.138182055891484 37.0 35.0 37.0 32.0 37.0 9 36.6345219807158 39.0 35.0 39.0 32.0 39.0 10-11 36.58235038404968 38.5 35.0 39.0 32.0 39.0 12-13 36.480611537832985 38.0 35.0 39.0 32.0 39.0 14-15 37.556599771204446 39.0 36.0 41.0 32.0 41.0 16-17 37.510992645857165 39.0 36.0 41.0 32.0 41.0 18-19 37.52343062591927 39.0 36.0 41.0 32.0 41.0 20-21 37.50804739336493 39.0 36.0 41.0 32.0 41.0 22-23 37.41541036117013 39.0 36.0 41.0 32.0 41.0 24-25 37.305052459552215 39.0 36.0 41.0 31.5 41.0 26-27 37.06238601078607 39.0 36.0 40.0 31.0 41.0 28-29 36.88503088739991 39.0 36.0 40.0 30.5 41.0 30-31 36.661644386337635 38.5 35.5 40.0 30.0 41.0 32-33 36.526773328975324 38.0 35.0 40.0 30.0 41.0 34-35 36.62856872037915 38.5 35.0 40.0 30.0 41.0 36-37 36.72061219153456 39.0 35.0 40.0 30.0 41.0 38-39 36.717457427684266 39.0 35.0 40.0 30.0 41.0 40-41 36.621344991011604 39.0 35.0 40.0 30.0 41.0 42-43 36.50521130903742 39.0 35.0 40.0 30.0 41.0 44-45 36.370907664651085 39.0 35.0 40.0 30.0 41.0 46-47 36.17085536852427 38.5 35.0 40.0 30.0 41.0 48-49 35.99997973525086 38.5 35.0 40.0 29.0 41.0 50-51 35.75824971400556 38.0 35.0 40.0 28.5 41.0 52-53 35.56779081549273 38.0 34.0 40.0 28.0 41.0 54-55 35.43221833632947 38.0 34.0 40.0 27.0 41.0 56-57 35.282959307076325 38.0 34.0 40.0 27.0 41.0 58-59 35.09132407256088 38.0 34.0 40.0 27.0 41.0 60-61 34.80388821702893 38.0 33.5 40.0 26.0 41.0 62-63 34.57098316718418 37.5 33.0 40.0 26.0 41.0 64-65 34.27367314920739 37.0 33.0 40.0 26.0 41.0 66-67 33.966171269815334 37.0 32.5 39.5 25.5 41.0 68-69 33.634164079097886 36.0 32.0 39.0 25.0 40.5 70-71 33.26967347605818 36.0 32.0 39.0 24.5 40.0 72-73 32.84265533583919 35.0 31.0 38.5 23.5 40.0 74-75 32.32263866644877 35.0 31.0 38.0 22.0 39.5 76-77 30.921117176009155 33.5 29.5 36.0 21.5 39.0 78-79 31.660848504657622 34.0 30.5 36.0 22.0 39.0 80-81 31.612476548455632 34.0 31.0 36.0 22.0 39.0 82-83 31.329096584409218 34.0 31.0 36.0 22.0 37.5 84-85 30.979621179931364 34.0 31.0 35.5 20.5 37.0 86-87 30.61701160320314 34.0 30.0 35.0 20.0 37.0 88-89 30.254212126164404 34.0 30.0 35.0 18.5 36.0 90-91 29.908533747344336 34.0 30.0 35.0 18.0 36.0 92-93 29.591508416407912 34.0 29.5 35.0 13.0 35.5 94-95 29.202070272920412 34.0 29.0 35.0 4.5 35.0 96-97 28.776248406602384 34.0 29.0 35.0 2.0 35.0 98-99 28.28502957999673 34.0 29.0 35.0 2.0 35.0 100-101 27.15504559568557 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2616504547103027 1101 2 0.43373149548889245 1101 3 0.32659930947052374 1101 4 0.24156501428951316 1101 5 0.22169673345797491 1101 6 0.23292911556693952 1101 7 0.2658408933845564 1101 8 0.4400171081028361 1101 9 0.6206646157396207 1101 10-11 0.4726341624450825 1101 12-13 0.3481100209499246 1101 14-15 0.3682251230857716 1101 16-17 0.4872198018195064 1101 18-19 0.46904392850101573 1101 20-21 0.42778657079868765 1101 22-23 0.4881434866857859 1101 24-25 0.5665955145979851 1101 26-27 0.3869033787985714 1101 28-29 0.5667408796234028 1101 30-31 0.4430031122435665 1101 32-33 0.5575916334508264 1101 34-35 0.4962245129502776 1101 36-37 0.5160137220008068 1101 38-39 0.6026739978696156 1101 40-41 0.7045945514840142 1101 42-43 0.6490271613918281 1101 44-45 0.6127912644237412 1101 46-47 0.3851082156902166 1101 48-49 0.4801681411944969 1101 50-51 0.5866410779924749 1101 52-53 0.31811005131782366 1101 54-55 0.241525993502421 1101 56-57 0.19236768055879594 1101 58-59 0.3145733439177718 1101 60-61 0.3321327880635394 1101 62-63 0.2546145304727574 1101 64-65 0.44158383676672486 1101 66-67 0.383223540888757 1101 68-69 0.4589078072349295 1101 70-71 0.5386116075265264 1101 72-73 0.43727550645962765 1101 74-75 0.4876630375363433 1101 76-77 0.268891361679934 1101 78-79 0.37877165617888764 1101 80-81 0.3742292478688398 1101 82-83 0.4142094036725332 1101 84-85 0.48264267596347565 1101 86-87 0.38357021726345053 1101 88-89 0.4078601096029395 1101 90-91 0.16808170700695158 1101 92-93 0.023098270524343434 1101 94-95 0.2689317268676561 1101 96-97 0.2792179591151829 1101 98-99 0.4628150957119139 1101 100-101 0.7453120412342145 1102 1 0.33315397146022363 1102 2 0.27013738479040583 1102 3 0.3094239815364901 1102 4 -0.019405238098094912 1102 5 0.27314551089518346 1102 6 0.25438051802056094 1102 7 0.2829714416269553 1102 8 0.35751940499219614 1102 9 0.4865347700751457 1102 10-11 0.4420345778153276 1102 12-13 0.4989513773113501 1102 14-15 0.46921413662004596 1102 16-17 0.42891818743441235 1102 18-19 0.5869648769039983 1102 20-21 0.46798406468008125 1102 22-23 0.5387806322120667 1102 24-25 0.4835675967764459 1102 26-27 0.536933682296791 1102 28-29 0.6215297974217933 1102 30-31 0.546825792385313 1102 32-33 0.5823569261015535 1102 34-35 0.5692908795415406 1102 36-37 0.6520546182122899 1102 38-39 0.5787944552401143 1102 40-41 0.6112653744266581 1102 42-43 0.46025219478357826 1102 44-45 0.7932848833176891 1102 46-47 0.5204274254058632 1102 48-49 0.6516027512637521 1102 50-51 0.5104497837821853 1102 52-53 0.5035112927961691 1102 54-55 0.5476063807695439 1102 56-57 0.5295537401775547 1102 58-59 0.6089274354690772 1102 60-61 0.3861162504601836 1102 62-63 0.13636680613036845 1102 64-65 0.3966120942962732 1102 66-67 0.322283205967544 1102 68-69 0.4380667210957867 1102 70-71 0.3886749167457353 1102 72-73 0.3792280940309496 1102 74-75 0.5805293624361738 1102 76-77 0.7408141089912235 1102 78-79 0.7770155974093989 1102 80-81 0.8171384223011842 1102 82-83 0.5263115013694666 1102 84-85 0.7055256774381178 1102 86-87 0.8454536279318248 1102 88-89 0.8367127072110883 1102 90-91 0.7181172219348007 1102 92-93 0.6830150420629906 1102 94-95 0.6981910090028762 1102 96-97 0.5367177854008638 1102 98-99 0.6493752047273027 1102 100-101 0.6848440162502385 1103 1 0.38190753372530395 1103 2 0.41736568219338466 1103 3 0.4425357680183488 1103 4 0.3239961688190931 1103 5 0.36063379447515587 1103 6 0.3429632809180916 1103 7 0.37824794913966286 1103 8 0.36321713555713586 1103 9 0.5378616842304709 1103 10-11 0.4820048488788444 1103 12-13 0.596087363526216 1103 14-15 0.6453457771082043 1103 16-17 0.6685447768437243 1103 18-19 0.587237549951972 1103 20-21 0.5513888800103643 1103 22-23 0.605332738327327 1103 24-25 0.6925175215073196 1103 26-27 0.6405740568435121 1103 28-29 0.6522291848542139 1103 30-31 0.6902025829856697 1103 32-33 0.8558053880551384 1103 34-35 0.6379296918989326 1103 36-37 0.6640474984160392 1103 38-39 0.680264806780059 1103 40-41 0.74031736219424 1103 42-43 0.6231156404998615 1103 44-45 0.7610488696461601 1103 46-47 0.7772825962525687 1103 48-49 0.7841644577425768 1103 50-51 0.8291477699781069 1103 52-53 0.8006851502677037 1103 54-55 0.8294556342324029 1103 56-57 0.7835392165506221 1103 58-59 0.898912381644557 1103 60-61 0.7625849451326374 1103 62-63 0.667899361209372 1103 64-65 0.7299624696951099 1103 66-67 0.8565358640563758 1103 68-69 0.6366721120691707 1103 70-71 0.6591798352751965 1103 72-73 0.5930918143138442 1103 74-75 0.650345970875783 1103 76-77 0.7693813446735227 1103 78-79 0.8656542204105087 1103 80-81 0.7132755541215516 1103 82-83 0.7659409311230228 1103 84-85 0.8335816511554661 1103 86-87 0.7082403843533704 1103 88-89 0.7642129202369041 1103 90-91 0.7399765112806698 1103 92-93 0.4998696448408424 1103 94-95 0.6672284163366449 1103 96-97 0.7756721600043974 1103 98-99 0.7196197583672053 1103 100-101 0.8011456986808128 1104 1 0.2540500314165186 1104 2 0.29628577885822693 1104 3 0.33281343058726165 1104 4 0.1310557990816008 1104 5 0.06343807029069382 1104 6 0.1528929942968773 1104 7 0.3395775884161125 1104 8 0.19398398416163332 1104 9 0.3493575007663523 1104 10-11 0.26221122942200026 1104 12-13 0.2842426023956506 1104 14-15 0.46556465550848003 1104 16-17 0.4581145961778006 1104 18-19 0.5104614440851662 1104 20-21 0.4175965545794398 1104 22-23 0.44980409556142575 1104 24-25 0.3924240640802239 1104 26-27 0.3504324328962909 1104 28-29 0.3349268417825044 1104 30-31 0.3708922877376253 1104 32-33 0.5066451223927331 1104 34-35 0.3826084250807469 1104 36-37 0.41175226142681254 1104 38-39 0.40416447000151834 1104 40-41 0.26901100432418446 1104 42-43 0.3652068748568027 1104 44-45 0.3189310911679186 1104 46-47 0.21826229552980436 1104 48-49 0.06325100816810902 1104 50-51 0.09071298833115549 1104 52-53 0.14849885477632796 1104 54-55 0.1725682642571158 1104 56-57 0.33328234687672875 1104 58-59 0.32386164366268133 1104 60-61 0.2561330646475497 1104 62-63 0.21431894634687865 1104 64-65 0.06807761588132166 1104 66-67 0.0257231162421121 1104 68-69 -0.07170808822834118 1104 70-71 0.17228323015770997 1104 72-73 0.026044128905923003 1104 74-75 0.01804011623802637 1104 76-77 0.15754487858087174 1104 78-79 0.40729475770776347 1104 80-81 0.13703309569352484 1104 82-83 0.2729944783577274 1104 84-85 0.21578326481683874 1104 86-87 0.28996475683375067 1104 88-89 0.34245005878062784 1104 90-91 0.30704299877451646 1104 92-93 0.2996672459158809 1104 94-95 0.4532407704131032 1104 96-97 0.5223229001982794 1104 98-99 0.49926650035281384 1104 100-101 0.8530400088378087 1105 1 0.3009964926006532 1105 2 0.34306562285703635 1105 3 0.4263871532938026 1105 4 0.13903291171013876 1105 5 0.22785000699055757 1105 6 0.3693049669831723 1105 7 0.33033339347760915 1105 8 0.2553984309152071 1105 9 0.4332562709300021 1105 10-11 0.4471415506908869 1105 12-13 0.4723555638091952 1105 14-15 0.5005130599600065 1105 16-17 0.487048588062855 1105 18-19 0.5122409732026867 1105 20-21 0.37341727016965365 1105 22-23 0.4635197847466017 1105 24-25 0.4184328987495505 1105 26-27 0.44998206268586927 1105 28-29 0.44307039166420026 1105 30-31 0.5239978269493335 1105 32-33 0.6395298848372875 1105 34-35 0.5580511031910902 1105 36-37 0.5707561569581898 1105 38-39 0.5559359921376767 1105 40-41 0.3291758221878496 1105 42-43 0.43807022537365725 1105 44-45 0.49649822835718993 1105 46-47 0.5881137615271186 1105 48-49 0.6493431514301022 1105 50-51 0.5234131403738402 1105 52-53 0.4659816037799587 1105 54-55 0.4505738961339034 1105 56-57 0.5924186724819762 1105 58-59 0.6363161882993538 1105 60-61 0.6097454727696814 1105 62-63 0.4979591539885959 1105 64-65 0.38293065628509027 1105 66-67 0.5999006912963196 1105 68-69 0.37756489585665776 1105 70-71 0.476034096081257 1105 72-73 0.5765424510384847 1105 74-75 0.6172162068518574 1105 76-77 0.7429766707952083 1105 78-79 0.7485215352126495 1105 80-81 0.5276176053333899 1105 82-83 0.7316143986477819 1105 84-85 0.8020119765924996 1105 86-87 0.8137933534528266 1105 88-89 0.8500790026687817 1105 90-91 0.8072036781983734 1105 92-93 0.7675952370632189 1105 94-95 0.9896109988369872 1105 96-97 1.0230289540159916 1105 98-99 1.05179283792355 1105 100-101 1.0460140176364945 1106 1 0.28559722293312717 1106 2 0.22417150285303222 1106 3 0.27962857279786846 1106 4 0.17592945401387539 1106 5 0.19874161363093634 1106 6 0.2176504269924635 1106 7 0.250105369473161 1106 8 0.29368715811508395 1106 9 0.38312013040019366 1106 10-11 0.2619379773434716 1106 12-13 0.3128505871670839 1106 14-15 0.33557794298842225 1106 16-17 0.35579272582432253 1106 18-19 0.4110252701438526 1106 20-21 0.33103485642993746 1106 22-23 0.27586131079543463 1106 24-25 0.27896707958424116 1106 26-27 0.2890034900214218 1106 28-29 0.36547866755139324 1106 30-31 0.39228354904053475 1106 32-33 0.5178699720765039 1106 34-35 0.5949585731627707 1106 36-37 0.534135174014466 1106 38-39 0.7028852604392384 1106 40-41 0.5589163811380686 1106 42-43 0.5630820181486484 1106 44-45 0.535049948098937 1106 46-47 0.6182003632955784 1106 48-49 0.683442939251222 1106 50-51 0.5562615280874965 1106 52-53 0.5219097725760022 1106 54-55 0.6412538702717612 1106 56-57 0.5997913879163903 1106 58-59 0.8145103911715097 1106 60-61 0.713347232124228 1106 62-63 0.6767980892694894 1106 64-65 0.6470290781477104 1106 66-67 0.7274105489634408 1106 68-69 0.7196797638595385 1106 70-71 0.6136478576577602 1106 72-73 0.547562822032809 1106 74-75 0.7328662664987178 1106 76-77 0.76103919526863 1106 78-79 0.5388880674082834 1106 80-81 0.5371024568537024 1106 82-83 0.6775945649304624 1106 84-85 0.49542550686669173 1106 86-87 0.48290793915761654 1106 88-89 0.4810418212785983 1106 90-91 0.6167065603707975 1106 92-93 0.6649971840093905 1106 94-95 0.8783321884957687 1106 96-97 0.5616894040537836 1106 98-99 0.5686063299509065 1106 100-101 0.647860429263531 1107 1 0.13006542508281171 1107 2 0.2091100856062802 1107 3 0.09458187255890493 1107 4 0.041782152906677084 1107 5 0.07165342541135544 1107 6 0.13594340049050402 1107 7 0.15256978202079097 1107 8 0.1942446915138376 1107 9 0.12156219183537331 1107 10-11 0.00628990012547348 1107 12-13 0.03921837035236564 1107 14-15 0.1743344747815172 1107 16-17 0.1683618776437399 1107 18-19 0.23481078759801477 1107 20-21 0.22158566634116994 1107 22-23 0.0966168668227283 1107 24-25 0.13434511929051496 1107 26-27 0.09930634708271668 1107 28-29 0.27738048486943967 1107 30-31 0.5031513693751819 1107 32-33 0.18453413771805316 1107 34-35 0.38451798891843225 1107 36-37 0.43764654007559756 1107 38-39 0.33239401396613744 1107 40-41 0.34527857178718335 1107 42-43 0.30658243337981617 1107 44-45 0.5686086420953416 1107 46-47 0.42972036557944193 1107 48-49 0.39474601115666985 1107 50-51 0.4388751822938133 1107 52-53 0.30710221547850836 1107 54-55 0.3254355208238948 1107 56-57 0.44652570182237383 1107 58-59 0.5214805285656254 1107 60-61 0.4904764310556402 1107 62-63 0.42115678311948557 1107 64-65 0.3619824877098523 1107 66-67 0.29289391354739536 1107 68-69 0.3864599526661934 1107 70-71 0.4513122344834315 1107 72-73 0.16869100640203527 1107 74-75 0.44699031173237813 1107 76-77 0.34293727568915244 1107 78-79 0.5113903942766491 1107 80-81 0.5600172149637075 1107 82-83 0.5449465978795764 1107 84-85 0.4653132864797769 1107 86-87 0.4811503895083007 1107 88-89 0.6295761778606099 1107 90-91 0.6896972093428921 1107 92-93 0.5365536841797649 1107 94-95 0.7092156152271443 1107 96-97 0.662149726876347 1107 98-99 0.7627582866248552 1107 100-101 0.6110578446919774 1108 1 0.12222538076209588 1108 2 0.12371519066707037 1108 3 0.06964684770851193 1108 4 -0.1388713429501678 1108 5 0.05094991940242011 1108 6 0.0459621080708601 1108 7 0.20555444294279113 1108 8 0.1228643025056897 1108 9 0.17919050019859384 1108 10-11 0.14533334217802718 1108 12-13 0.20431143354174708 1108 14-15 0.14373152601738326 1108 16-17 0.18555251183462929 1108 18-19 0.08661215228062247 1108 20-21 0.1971605572906654 1108 22-23 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2316 94-95 -1.4975213677492647 2316 96-97 -1.44616884031608 2316 98-99 -1.5899709011077583 2316 100-101 -2.0272536581939455 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 11.0 5 27.0 6 49.0 7 132.0 8 408.0 9 783.0 10 1301.0 11 2137.0 12 3134.0 13 4095.0 14 5094.0 15 6518.0 16 7930.0 17 9030.0 18 7965.0 19 6719.0 20 6709.0 21 7115.0 22 7958.0 23 9471.0 24 11740.0 25 15058.0 26 19694.0 27 25765.0 28 32836.0 29 41579.0 30 51366.0 31 62333.0 32 76075.0 33 92079.0 34 113724.0 35 142204.0 36 185504.0 37 257521.0 38 263582.0 39 52084.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.28271178640211 22.9630026711504 15.526012664103165 22.22827287834433 2 14.77066055460908 23.057894138151504 40.43149627301337 21.73994903422604 3 17.564890875597 27.50878917868737 32.06922565015081 22.857094295564814 4 12.249718262176934 19.479362909092814 41.0642453911883 27.20667343754195 5 11.904466176816673 39.172005334309546 36.80510158721857 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28.871163646918234 30.22433039508194 20.262396483511885 28-29 20.637471633371934 28.970845823147823 30.191440215748166 20.20024232773208 30-31 20.96624253831113 28.697372661797132 30.111751273333 20.22463352655874 32-33 20.774155203730558 28.85390690077689 29.98573971081886 20.386198184673688 34-35 20.757390120702798 28.96618726389991 30.087666765606958 20.18875584979033 36-37 20.875955066756617 28.814873939032616 30.05574164430987 20.253429349900898 38-39 20.765917501390753 28.83453930617285 30.17508777595249 20.224455416483902 40-41 20.89004436428461 28.81972306723806 30.07808937770699 20.212143190770345 42-43 20.847854050246227 28.759829167395356 29.89243691435651 20.4998798680019 44-45 20.81206725561199 28.928849974536273 29.844498037756917 20.414584732094816 46-47 20.919560143567885 28.915559071385143 29.562627893749305 20.602252891297667 48-49 20.952143727357093 28.958208095565446 29.508976422120824 20.580671754956636 50-51 20.924951009158928 29.054450569027306 29.339095215140524 20.68150320667323 52-53 21.012105421948924 29.09663198718842 29.232103811993902 20.659158778868754 54-55 20.99649851862918 29.084884525649144 29.307931929188413 20.61068502653326 56-57 20.957574148975883 29.15712476718637 29.23302413547612 20.652276948361624 58-59 20.946685906283367 29.04311456877912 29.355409638420305 20.654789886517207 60-61 21.000589710243485 29.133616821861036 29.325321738971077 20.540471728924402 62-63 20.93929655307534 29.076610011339366 29.278693820422586 20.705399615162705 64-65 20.768820197200093 28.901972361319217 29.412354477860127 20.91685296362057 66-67 20.803105675748917 29.001446077812947 29.281355361252388 20.91409288518575 68-69 20.78775004251546 29.02500171208694 29.261122257425132 20.926125987972465 70-71 20.849718749109268 28.838495578845425 29.297795041505353 21.013990630539958 72-73 20.822372911763466 28.948411730562036 29.274351615016414 20.954863742658087 74-75 20.809009269447206 29.001921015171796 29.138270155500294 21.050799559880705 76-77 20.765188953559786 29.020538879023928 29.144257594353945 21.070014573062338 78-79 20.69439477845774 29.017535234885067 29.184271306817255 21.10379867983994 80-81 20.77571498610884 29.03748978591273 29.046347442392552 21.14044778558588 82-83 20.884785095603856 28.917208694231082 29.1230266383396 21.074979571825462 84-85 20.8417388462167 28.89681320477202 29.058179441085148 21.203268507926133 86-87 20.68730184670698 28.933747344337313 29.13149207386828 21.24745873508743 88-89 20.776336002614805 28.8168981859781 29.04938715476385 21.35737865664324 90-91 20.685906334669024 28.817630213585353 29.231085538412056 21.265377913333566 92-93 20.735062925207316 28.779656019109833 29.09283548042154 21.392445575261306 94-95 20.567653831508107 28.752923333883544 29.086220168102933 21.59320266650542 96-97 20.54711554175519 28.735120117666284 29.10942964536689 21.608334695211635 98-99 20.465598954077464 28.692008498120607 29.09841477365583 21.743977774146103 100-101 20.526818107533913 28.69380617747998 28.998496486353982 21.78087922863213 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 54.0 1 40.5 2 31.5 3 49.5 4 50.0 5 46.0 6 92.5 7 201.0 8 368.0 9 490.0 10 486.0 11 458.5 12 509.0 13 613.0 14 706.0 15 844.0 16 986.0 17 1138.0 18 1332.0 19 1484.0 20 1720.0 21 2094.0 22 2585.0 23 3384.5 24 4568.5 25 6199.0 26 8395.5 27 11376.0 28 15353.0 29 20346.5 30 26061.0 31 32284.5 32 38524.0 33 44100.0 34 49341.0 35 53882.0 36 58866.0 37 63427.5 38 64988.0 39 65418.5 40 65855.0 41 65568.5 42 65100.5 43 65325.5 44 65312.5 45 64889.0 46 64116.0 47 62471.5 48 59955.5 49 56978.5 50 52782.0 51 47141.0 52 42093.0 53 37326.5 54 32119.0 55 26483.5 56 20821.0 57 15761.0 58 11739.5 59 8731.0 60 6102.0 61 4015.0 62 2643.5 63 1624.0 64 907.5 65 500.5 66 241.5 67 125.5 68 61.0 69 35.0 70 22.5 71 13.0 72 7.5 73 3.5 74 1.5 75 3.5 76 2.5 77 1.5 78 1.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004706651413629678 2 0.006275535218172904 3 0.0018303644386337635 4 0.1713351854878248 5 0.0019611047556790328 6 3.268507926131721E-4 7 0.0 8 6.537015852263442E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.249060303971237E-4 24-25 7.84441902271613E-4 26-27 0.0027455466579506455 28-29 2.2879555482922044E-4 30-31 0.006733126327831345 32-33 0.021441411995424087 34-35 0.013596992972707958 36-37 9.151822193168818E-4 38-39 1.9611047556790325E-4 40-41 0.0018630495178950809 42-43 0.011930053930380782 44-45 0.008040529498284033 46-47 0.010459225363621507 48-49 0.01042654028436019 50-51 0.19179604510540937 52-53 0.11485536852426867 54-55 0.016636705344010457 56-57 0.006079424742605001 58-59 0.01745383232554339 60-61 0.12296126818107533 62-63 0.23863376368687697 64-65 0.2442555973198235 66-67 0.25484556300049027 68-69 0.25066187285504166 70-71 0.23673802908972053 72-73 0.1617584572642589 74-75 0.27811733943454814 76-77 0.21595031867952277 78-79 0.005687203791469194 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.005262297761072071 92-93 0.018009478672985784 94-95 0.0021245301519856184 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1529750.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5103927475043 #Duplication Level Percentage of deduplicated Percentage of total 1 80.23224890259769 55.76975132235087 2 13.046953769550163 18.137977613599272 3 3.3579108091854772 7.002290974727174 4 1.3001785799307934 3.6150369493152748 5 0.6273582085704726 2.1803957735552135 6 0.35825817365694324 1.4941599813538633 7 0.22948351731891625 1.11660425925416 8 0.15196861113867466 0.8450718276433625 9 0.12717458493106829 0.795595981145346 >10 0.5364642369034415 6.567853308267056 >50 0.02332459538784633 1.1102350625586117 >100 0.008298837355399073 0.9199852869089044 >500 9.429336827334938E-5 0.03618011722315398 >1k 2.828801048200481E-4 0.40886154209788866 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2703 0.17669553848668082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2289 0.1496322928583102 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.537015852263442E-5 0.0 8 0.0 0.0 0.0 6.537015852263442E-5 0.0 9 0.0 0.0 0.0 1.3074031704526885E-4 0.0 10-11 0.0 0.0 0.0 1.3074031704526885E-4 0.0 12-13 0.0 0.0 0.0 1.3074031704526885E-4 0.0 14-15 0.0 0.0 0.0 1.3074031704526885E-4 0.0 16-17 0.0 0.0 0.0 1.3074031704526885E-4 0.0 18-19 0.0 0.0 0.0 1.3074031704526885E-4 0.0 20-21 0.0 0.0 0.0 1.6342539630658605E-4 0.0 22-23 0.0 0.0 0.0 1.9611047556790325E-4 0.0 24-25 0.0 0.0 0.0 4.902761889197582E-4 0.0 26-27 0.0 0.0 0.0 6.21016505965027E-4 0.0 28-29 0.0 0.0 0.0 0.0010459225363621508 0.0 30-31 0.0 0.0 0.0 0.003268507926131721 0.0 32-33 0.0 0.0 0.0 0.00761562346788691 0.0 34-35 0.0 0.0 0.0 0.015721523124693576 0.0 36-37 0.0 0.0 0.0 0.030527864030070274 0.0 38-39 0.0 0.0 0.0 0.05301519856185651 0.0 40-41 0.0 0.0 0.0 0.08328158195783625 0.0 42-43 0.0 0.0 0.0 0.11433240725608759 0.0 44-45 0.0 0.0 0.0 0.1536525576074522 0.0 46-47 0.0 0.0 0.0 0.2003922209511358 0.0 48-49 0.0 0.0 0.0 0.2539630658604347 0.0 50-51 0.0 0.0 0.0 0.3109004739336493 0.0 52-53 0.0 0.0 0.0 0.3685569537506128 0.0 54-55 0.0 0.0 0.0 0.4406602386010786 0.0 56-57 0.0 0.0 0.0 0.5294002287955548 0.0 58-59 0.0 0.0 0.0 0.6270959307076319 0.0 60-61 0.0 0.0 0.0 0.7374407582938389 0.0 62-63 0.0 0.0 0.0 0.8533420493544697 0.0 64-65 0.0 0.0 0.0 0.9721523124693576 0.0 66-67 0.0 0.0 0.0 1.1152149043961432 0.0 68-69 0.0 0.0 0.0 1.2749468867462004 0.0 70-71 0.0 0.0 0.0 1.4584082366399738 0.0 72-73 0.0 0.0 0.0 1.6446805033502208 0.0 74-75 0.0 0.0 0.0 1.8358882170289261 0.0 76-77 0.0 0.0 0.0 2.053472789671515 0.0 78-79 0.0 0.0 0.0 2.311096584409217 0.0 80-81 0.0 0.0 0.0 2.590227161300866 0.0 82-83 0.0 0.0 0.0 2.8892302663833958 6.537015852263442E-5 84-85 0.0 0.0 0.0 3.1832979244974666 6.537015852263442E-5 86-87 0.0 0.0 0.0 3.4898839679686224 6.537015852263442E-5 88-89 0.0 0.0 0.0 3.8422291224056218 6.537015852263442E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1990 0.0 52.557037 1 GTATCAA 3480 0.0 41.119568 1 TCAACGC 4170 0.0 34.083134 4 CAACGCA 4240 0.0 33.224392 5 ATCAACG 4235 0.0 33.112137 3 AACGCAG 4450 0.0 32.188385 6 TATCAAC 4425 0.0 31.692448 2 ACGCAGA 5210 0.0 27.219849 7 CGCAGAG 5210 0.0 27.12881 8 GTGGTAT 1015 0.0 22.950459 1 TAGACCG 125 2.2785465E-4 22.767097 5 GCAGAGT 6135 0.0 22.264656 9 TGGTATC 1025 0.0 20.870636 2 GAGTACT 4185 0.0 20.85261 12-13 TAACGCC 115 0.0037084224 20.666958 4 GCGTGCA 255 1.2134478E-8 20.45929 9 AGAGTAC 5490 0.0 19.567436 10-11 CCTATAC 390 0.0 19.501768 3 CAGAGTA 5865 0.0 19.48889 10-11 GTACTTT 4455 0.0 19.269432 14-15 >>END_MODULE