FastQCFastQC Report
Sat 18 Jun 2016
SRR1931028_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931028_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876208
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN824384.393862514177532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.053.7057571
GTATCAA27750.043.690361
ATCAACG30950.035.7544943
CAACGCA32150.035.1840825
TCAACGC32050.034.9912344
TATCAAC34800.032.7786332
AACGCAG34600.031.3772536
CGCAGAG39650.026.892948
ACGCAGA40100.026.880387
ACATGGG32300.023.6706223
TACATGG34950.023.5637232
GTACATG36300.023.5347771
GTGGTAT9350.023.526491
GAGTACA23500.023.0748161
GCAGAGT42350.022.981619
GTGTAGC6250.022.9180811
GAGTACT28050.021.05766912-13
AGTACAT24850.020.9961172
AGAGTAC38950.020.30746710-11
TGGTATC10100.020.2583392
Produced by FastQC (version 0.11.5)