FastQCFastQC Report
Sat 18 Jun 2016
SRR1931028_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931028_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876208
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39080.20829247077083138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36930.1968331869387616No Hit
GTATAATGGAGGCTGACCAGAGCAGTTTAGGAGATTGTAAAGGGAGGTTT23230.12381356438092152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21480.11448624033156239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.052.356591
GTATCAA52100.039.3985631
ATCAACG59550.033.5883263
CAACGCA59900.033.349355
TCAACGC60800.032.8977784
TATCAAC64250.031.2808422
AACGCAG64750.031.2177736
ACGCAGA72000.027.8107097
CGCAGAG72400.027.5915228
GGCGTCG2950.027.343818
GCGTGCG6950.027.3090939
GGCGTGC6700.026.2034858
ACGGGCG3100.024.4900915
GCAGAGT80700.024.0481629
TACGGGC3400.023.7551614
GCGTCGG3500.023.0469259
ACATGGG48750.022.317673
AAAGGCG8450.021.8997945
TACATGG52500.021.4486642
TGGTATC15600.021.3198742
Produced by FastQC (version 0.11.5)