FastQCFastQC Report
Sat 18 Jun 2016
SRR1931027_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931027_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342566
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1921654.425148633319562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45500.042.5811351
ATCAACG46850.038.6079443
GGTATCA24700.037.9744071
TCAACGC46900.037.592774
CAACGCA49050.035.9393775
TATCAAC54650.033.5719222
AACGCAG52550.032.587096
ACGCAGA63300.026.206117
GTACATG59750.025.4774171
CGCAGAG64350.025.2260688
TACATGG64450.022.4238152
ACATGGG64350.021.5315823
GCAGAGT75350.020.650879
GAGTACA49250.019.4613151
GTGGTAT18200.017.742141
TATACTG21050.017.4667575
GTATAGG10050.017.3400251
AGAGTAC67850.016.94176910-11
AGTACAT50550.016.5253142
CAGAGTA73450.015.7124410-11
Produced by FastQC (version 0.11.5)