##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931027_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4342566 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.964164735780642 28.0 2.0 31.0 2.0 31.0 2 20.289297387765668 28.0 2.0 31.0 2.0 33.0 3 21.06713519149738 30.0 2.0 31.0 2.0 33.0 4 22.65824975371704 32.0 2.0 35.0 2.0 37.0 5 21.81315079609613 31.0 2.0 35.0 2.0 37.0 6 22.459377243777066 31.0 2.0 35.0 2.0 37.0 7 22.186298607781666 30.0 2.0 35.0 2.0 37.0 8 21.929523466079733 29.0 2.0 35.0 2.0 37.0 9 20.872002636229364 26.0 2.0 34.0 2.0 37.0 10-11 20.811636599190432 26.0 2.0 33.5 2.0 37.0 12-13 20.43106161195938 25.5 2.0 33.0 2.0 37.0 14-15 22.04935365403773 28.5 2.0 35.0 2.0 37.5 16-17 23.309634557079846 31.5 2.0 36.5 2.0 38.5 18-19 23.413784845181397 32.0 2.0 37.5 2.0 39.5 20-21 23.68304546206091 32.0 2.0 38.0 2.0 40.0 22-23 23.64679419955851 32.0 2.0 38.0 2.0 40.0 24-25 23.713100618390143 32.0 2.0 38.0 2.0 40.0 26-27 23.576988352048076 32.0 2.0 38.0 2.0 40.0 28-29 23.5263135897071 32.0 2.0 38.0 2.0 40.0 30-31 23.708229420117046 32.0 2.0 38.0 2.0 40.0 32-33 23.679885003474904 32.0 2.0 38.0 2.0 40.0 34-35 23.447795381808817 31.0 2.0 38.0 2.0 40.0 36-37 22.972366453382634 31.0 2.0 38.0 2.0 40.0 38-39 23.008981671205458 30.5 2.0 38.0 2.0 40.0 40-41 23.022762463483573 30.5 2.0 38.0 2.0 40.0 42-43 22.978394801598867 31.0 2.0 38.0 2.0 40.0 44-45 22.673635587806842 30.0 2.0 38.0 2.0 40.0 46-47 22.411357017947452 30.0 2.0 38.0 2.0 40.0 48-49 22.34312673658846 30.0 2.0 38.0 2.0 40.0 50-51 19.522211637082776 20.5 2.0 36.0 2.0 39.0 52-53 21.81202001305219 28.5 2.0 37.0 2.0 39.0 54-55 22.803333674145655 31.0 2.0 38.0 2.0 40.0 56-57 22.872688290747913 31.0 2.0 38.0 2.0 40.0 58-59 22.62869660472633 31.0 2.0 38.0 2.0 40.0 60-61 22.391930485339774 31.0 2.0 38.0 2.0 40.0 62-63 21.982009714993396 29.5 2.0 37.5 2.0 40.0 64-65 21.707824475206593 29.0 2.0 37.0 2.0 40.0 66-67 21.433119381490116 29.0 2.0 36.5 2.0 39.0 68-69 21.035919546185365 28.0 2.0 36.0 2.0 39.0 70-71 20.743995946175602 28.0 2.0 35.5 2.0 39.0 72-73 20.377508942869262 27.5 2.0 35.0 2.0 38.5 74-75 19.974335680793338 26.0 2.0 35.0 2.0 38.0 76-77 19.62892953152583 26.0 2.0 34.0 2.0 37.0 78-79 19.321130640271214 26.0 2.0 34.0 2.0 36.5 80-81 18.94558654951934 25.5 2.0 34.0 2.0 36.0 82-83 18.539087258547134 24.5 2.0 34.0 2.0 36.0 84-85 18.219823947408052 24.0 2.0 34.0 2.0 35.0 86-87 17.778211085335258 21.0 2.0 33.5 2.0 35.0 88-89 17.41893859529136 20.0 2.0 33.0 2.0 35.0 90-91 16.992164310225796 18.0 2.0 33.0 2.0 35.0 92-93 16.484356138743774 10.0 2.0 32.0 2.0 35.0 94-95 15.959087323025143 4.5 2.0 32.0 2.0 34.0 96-97 15.335118453006817 2.0 2.0 32.0 2.0 34.0 98-99 14.642070379586631 2.0 2.0 31.5 2.0 34.0 100-101 13.43424417729057 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 9.463654460522033 1101 2 10.45099070293968 1101 3 9.84296956613213 1101 4 12.09475641874107 1101 5 12.922790434137777 1101 6 12.237502147810382 1101 7 12.35028558660289 1101 8 12.177308991274064 1101 9 13.297642412220302 1101 10-11 12.815907312766502 1101 12-13 12.282415479028241 1101 14-15 11.651474360087729 1101 16-17 11.336293948224892 1101 18-19 11.884037128803897 1101 20-21 11.992420614215739 1101 22-23 12.275151046217683 1101 24-25 12.400674964886143 1101 26-27 12.493349125266036 1101 28-29 12.568142330167024 1101 30-31 12.144645862248495 1101 32-33 12.276950428054942 1101 34-35 12.26601257295128 1101 36-37 12.871114465417907 1101 38-39 12.566125201419368 1101 40-41 12.127330387534474 1101 42-43 12.026228411134571 1101 44-45 11.942941041685604 1101 46-47 11.761722139953857 1101 48-49 12.073415454531084 1101 50-51 14.055844928358244 1101 52-53 12.600735727630632 1101 54-55 12.183973000236254 1101 56-57 12.165362085939044 1101 58-59 12.100339244694606 1101 60-61 11.870785333707335 1101 62-63 11.990428126322907 1101 64-65 11.850916237788066 1101 66-67 11.701848617919548 1101 68-69 11.762067096985682 1101 70-71 11.684138622279903 1101 72-73 11.722564616746148 1101 74-75 11.51815537570197 1101 76-77 11.286567503497011 1101 78-79 10.849083860461192 1101 80-81 10.845769432992427 1101 82-83 10.541492287465921 1101 84-85 10.456483170750762 1101 86-87 10.382154332347096 1101 88-89 10.259871138104092 1101 90-91 10.086881078542952 1101 92-93 9.864034696362829 1101 94-95 9.770564327438056 1101 96-97 9.72351280383076 1101 98-99 9.583193251249739 1101 100-101 8.875893104292112 1102 1 9.139970647549074 1102 2 9.951980254014277 1102 3 9.428964026480973 1102 4 11.504889941622402 1102 5 12.130762724465082 1102 6 11.347808219041347 1102 7 11.450344414305714 1102 8 11.417523317384425 1102 9 11.68935581009455 1102 10-11 10.937691743231564 1102 12-13 10.543900056404556 1102 14-15 9.941087952498094 1102 16-17 10.2552990271219 1102 18-19 11.205343853041725 1102 20-21 11.499881569379468 1102 22-23 11.900383563961814 1102 24-25 11.873095771238518 1102 26-27 12.244310185016886 1102 28-29 12.134681727092858 1102 30-31 12.000976272511323 1102 32-33 12.00578863749909 1102 34-35 11.968289223399122 1102 36-37 12.252331588265161 1102 38-39 12.15104083957813 1102 40-41 11.830395493318331 1102 42-43 11.519034224465539 1102 44-45 11.569069175782179 1102 46-47 11.652472159016032 1102 48-49 11.199950520164084 1102 50-51 13.502271657643977 1102 52-53 12.132166397791426 1102 54-55 11.693781839254061 1102 56-57 11.500709783044982 1102 58-59 11.739922979828659 1102 60-61 11.651686213054578 1102 62-63 11.660853107801 1102 64-65 11.610367610081088 1102 66-67 11.417817948818904 1102 68-69 11.233398250367472 1102 70-71 11.022405278440285 1102 72-73 11.099193362259399 1102 74-75 10.77410463423438 1102 76-77 10.6031755548291 1102 78-79 10.290542192180528 1102 80-81 10.039571876905804 1102 82-83 10.00208300180202 1102 84-85 9.851611016774068 1102 86-87 9.786413036013272 1102 88-89 9.566668617493502 1102 90-91 9.322558250540027 1102 92-93 9.25012177135281 1102 94-95 9.131572793897398 1102 96-97 9.113054005852698 1102 98-99 8.615786637552127 1102 100-101 7.927426722423158 1103 1 8.955234888113388 1103 2 9.971654293316945 1103 3 9.297350543336695 1103 4 11.59943360142632 1103 5 12.091885792673512 1103 6 11.172358662722019 1103 7 11.204125638861196 1103 8 10.856088949699103 1103 9 10.98885704776367 1103 10-11 10.16508727730551 1103 12-13 9.794351385375176 1103 14-15 9.596392770843163 1103 16-17 10.238544932894925 1103 18-19 11.407216494817973 1103 20-21 11.933859495488989 1103 22-23 12.242494633312713 1103 24-25 12.33217020725656 1103 26-27 12.455212468510432 1103 28-29 12.324599802718541 1103 30-31 12.119422541657954 1103 32-33 12.288893373505505 1103 34-35 12.292725166752358 1103 36-37 12.628680496232288 1103 38-39 12.443639368118212 1103 40-41 12.064233270429536 1103 42-43 11.76019252859992 1103 44-45 11.772363449042953 1103 46-47 11.72756901250023 1103 48-49 11.863410032410112 1103 50-51 13.825053234339624 1103 52-53 12.545105691654786 1103 54-55 12.067745734529797 1103 56-57 11.985280496694472 1103 58-59 12.10860335347169 1103 60-61 11.924789336745011 1103 62-63 11.934875520486965 1103 64-65 11.688239335058025 1103 66-67 11.467968768580501 1103 68-69 11.312460240979185 1103 70-71 11.275765025273813 1103 72-73 11.160435476948745 1103 74-75 10.925227140771284 1103 76-77 10.772195665060124 1103 78-79 10.45753794458934 1103 80-81 10.292038949410028 1103 82-83 10.108264017782172 1103 84-85 9.885918168047748 1103 86-87 9.732875257229 1103 88-89 9.691501933498671 1103 90-91 9.580863047175097 1103 92-93 9.337321700327252 1103 94-95 9.26968946741566 1103 96-97 9.102276310865035 1103 98-99 8.758321121595081 1103 100-101 8.04329485449484 1104 1 8.786796329396314 1104 2 9.868467434335166 1104 3 9.275051308892841 1104 4 11.311135729085898 1104 5 11.770537136641291 1104 6 10.903224871752805 1104 7 10.723470112116889 1104 8 10.394356194005667 1104 9 9.90772695531042 1104 10-11 9.107759869893815 1104 12-13 8.604139236801718 1104 14-15 9.109412000425117 1104 16-17 10.02003223986597 1104 18-19 11.263392722254522 1104 20-21 11.73856798895629 1104 22-23 12.104875762778132 1104 24-25 12.187416451825513 1104 26-27 12.373763506873132 1104 28-29 12.263699695448071 1104 30-31 11.928789317910272 1104 32-33 12.071990581579339 1104 34-35 12.000989116736537 1104 36-37 12.365704724695231 1104 38-39 12.030947569099858 1104 40-41 11.632075691848133 1104 42-43 11.378130186322235 1104 44-45 11.536713912852672 1104 46-47 11.550576043925066 1104 48-49 11.413030389489581 1104 50-51 13.493529897224821 1104 52-53 12.072990249530672 1104 54-55 11.672062985010204 1104 56-57 11.501224100596339 1104 58-59 11.384450930046594 1104 60-61 11.39841999834913 1104 62-63 11.330330030862878 1104 64-65 11.237693963122755 1104 66-67 11.122018494037558 1104 68-69 10.872310071970325 1104 70-71 10.742783528464916 1104 72-73 10.638618670213084 1104 74-75 10.417657048395203 1104 76-77 10.174793308196747 1104 78-79 9.864816853594657 1104 80-81 9.873152304969185 1104 82-83 9.631231859793612 1104 84-85 9.36805553695012 1104 86-87 9.217908446441758 1104 88-89 9.123812700482521 1104 90-91 8.757563723017633 1104 92-93 8.731426237906646 1104 94-95 8.455589526453004 1104 96-97 8.06761056159239 1104 98-99 7.939192853318588 1104 100-101 7.186206828078523 1105 1 8.60961811192357 1105 2 9.417205968331878 1105 3 8.878235115124145 1105 4 10.961459703167968 1105 5 10.74696540758092 1105 6 10.47801576334546 1105 7 10.192092452579924 1105 8 9.594075580981368 1105 9 8.812036033870314 1105 10-11 8.104919191816176 1105 12-13 7.302495159405165 1105 14-15 8.297750226076534 1105 16-17 9.780463100718094 1105 18-19 10.72068760329951 1105 20-21 11.554956823660177 1105 22-23 12.06981429348458 1105 24-25 12.107184041256225 1105 26-27 12.238688641480422 1105 28-29 11.996089700547987 1105 30-31 11.713518822466128 1105 32-33 11.816111456697147 1105 34-35 11.875360146379517 1105 36-37 11.999990982882718 1105 38-39 11.794398897781377 1105 40-41 11.404288539412274 1105 42-43 11.263916220066303 1105 44-45 11.314810468175036 1105 46-47 11.207506410061615 1105 48-49 11.29548126458388 1105 50-51 13.243775374866264 1105 52-53 11.626326348257574 1105 54-55 11.36311474330697 1105 56-57 11.342982444368445 1105 58-59 11.290576120118441 1105 60-61 11.06939570440581 1105 62-63 11.219137119984534 1105 64-65 11.039399380552258 1105 66-67 10.779348980503972 1105 68-69 10.626473556084193 1105 70-71 10.538439314522162 1105 72-73 10.429022245326422 1105 74-75 10.327202004058815 1105 76-77 10.048644357675354 1105 78-79 9.898893944619974 1105 80-81 9.610134586865414 1105 82-83 9.421696304901332 1105 84-85 9.283031553232252 1105 86-87 9.048709251147784 1105 88-89 8.772402349763244 1105 90-91 8.491373009569593 1105 92-93 8.49260786785938 1105 94-95 8.492353005396453 1105 96-97 8.128268098543296 1105 98-99 7.803469217194241 1105 100-101 7.180672358975331 1106 1 8.233838350775049 1106 2 9.24253517382935 1106 3 8.944122550011706 1106 4 10.710270677182784 1106 5 10.916100804084838 1106 6 10.154140424237639 1106 7 9.681304526686663 1106 8 8.876964461981615 1106 9 7.696589906238724 1106 10-11 7.406845894081382 1106 12-13 7.04029377046438 1106 14-15 8.163148243370099 1106 16-17 9.607742643268182 1106 18-19 11.22557131640869 1106 20-21 11.572385517599962 1106 22-23 11.875716077397232 1106 24-25 11.72744792879574 1106 26-27 12.07162866890799 1106 28-29 12.016483345872974 1106 30-31 11.520979941839549 1106 32-33 11.748668843455686 1106 34-35 11.823717108272394 1106 36-37 11.997523903432462 1106 38-39 11.688227042048865 1106 40-41 11.637806778368383 1106 42-43 11.371117029814673 1106 44-45 11.09394342646571 1106 46-47 10.987788971885731 1106 48-49 11.093957101049835 1106 50-51 13.289176587059945 1106 52-53 11.971284478250777 1106 54-55 11.293331024966381 1106 56-57 11.290033374065693 1106 58-59 11.192371896871524 1106 60-61 11.10619562892396 1106 62-63 11.177426718062367 1106 64-65 11.013521857328502 1106 66-67 10.802541012684653 1106 68-69 10.616739947229647 1106 70-71 10.365371902129684 1106 72-73 10.20376936244287 1106 74-75 9.889256166023436 1106 76-77 9.632482880697317 1106 78-79 9.376396382833224 1106 80-81 9.205212432194884 1106 82-83 9.036441703124144 1106 84-85 8.69938790043382 1106 86-87 8.595476563396023 1106 88-89 8.426260378491186 1106 90-91 8.20964956647618 1106 92-93 8.052245302746943 1106 94-95 7.986992346013906 1106 96-97 7.469603193119815 1106 98-99 7.032126537065395 1106 100-101 6.548962554929206 1107 1 7.713574533273938 1107 2 8.535450800007482 1107 3 8.470025208809854 1107 4 10.088913506863676 1107 5 10.500384668020665 1107 6 9.865106660151387 1107 7 9.3521577832758 1107 8 7.843935669844161 1107 9 4.779713648787336 1107 10-11 6.380645716218499 1107 12-13 6.43590386534947 1107 14-15 7.480472190843624 1107 16-17 9.152713980841867 1107 18-19 10.952010456694538 1107 20-21 11.288885216184894 1107 22-23 11.036986201285355 1107 24-25 11.247061744243865 1107 26-27 11.390016457585233 1107 28-29 11.444165893287522 1107 30-31 10.999135468280087 1107 32-33 11.389242460585766 1107 34-35 11.482101859469662 1107 36-37 11.932031215780587 1107 38-39 11.515189739368417 1107 40-41 11.031313290053578 1107 42-43 10.934071462015876 1107 44-45 10.381996588431047 1107 46-47 10.793208214111463 1107 48-49 11.006511061811754 1107 50-51 12.929763291949396 1107 52-53 11.412089312463163 1107 54-55 11.02006906074799 1107 56-57 10.801601017018555 1107 58-59 10.908518645881426 1107 60-61 10.955576421598924 1107 62-63 10.976790643624977 1107 64-65 10.998093675299945 1107 66-67 10.945612922107092 1107 68-69 10.733882439101706 1107 70-71 10.446793092821839 1107 72-73 10.262152293951658 1107 74-75 10.238647806841197 1107 76-77 10.04350783655821 1107 78-79 9.684533773045352 1107 80-81 9.455902304088813 1107 82-83 9.282235189945869 1107 84-85 9.11776155153142 1107 86-87 8.701091118850798 1107 88-89 8.777141737602506 1107 90-91 8.580457727452355 1107 92-93 8.111582427564507 1107 94-95 8.157830835131236 1107 96-97 7.887143776838551 1107 98-99 7.76655597607877 1107 100-101 7.3262901464455386 1108 1 6.843972895292023 1108 2 8.232645153979579 1108 3 8.276418264962214 1108 4 9.342309558463704 1108 5 10.27626276873109 1108 6 9.242258500770596 1108 7 8.53848680645817 1108 8 5.634390785527671 1108 9 5.270865626412469 1108 10-11 4.689637802396607 1108 12-13 3.8375141693019508 1108 14-15 6.748512099700086 1108 16-17 8.638384545257825 1108 18-19 8.850708000897974 1108 20-21 9.289001325244378 1108 22-23 10.481277379003483 1108 24-25 10.095345606676084 1108 26-27 8.78311917812152 1108 28-29 10.5661627476869 1108 30-31 10.677369932465837 1108 32-33 11.1607650184725 1108 34-35 10.981588433235242 1108 36-37 10.732482089816099 1108 38-39 10.292674753480725 1108 40-41 10.651186414266054 1108 42-43 9.647451335845876 1108 44-45 9.535984740878508 1108 46-47 10.385086461515098 1108 48-49 10.822006224049598 1108 50-51 12.779056016173772 1108 52-53 11.487328398176967 1108 54-55 10.8180522366975 1108 56-57 10.806752508895414 1108 58-59 10.74286132537129 1108 60-61 10.790864095978744 1108 62-63 10.927028155154584 1108 64-65 10.770990513300124 1108 66-67 10.261592656301762 1108 68-69 10.51708668932988 1108 70-71 10.632744588573615 1108 72-73 10.316352390409364 1108 74-75 10.222002555999268 1108 76-77 9.814200678275544 1108 78-79 9.667151734798555 1108 80-81 9.447028679597139 1108 82-83 9.603761449760302 1108 84-85 9.474896273329822 1108 86-87 9.200949061699951 1108 88-89 8.982128753177587 1108 90-91 8.889113457171504 1108 92-93 8.571419625779555 1108 94-95 8.42446837074067 1108 96-97 8.302750550466707 1108 98-99 8.144548327273391 1108 100-101 7.584910413773153 1109 1 6.592040263827265 1109 2 8.317373985640014 1109 3 8.211277842622025 1109 4 8.914210681039773 1109 5 9.483553424806036 1109 6 8.615740053538055 1109 7 6.863450505626908 1109 8 6.720378383867963 1109 9 4.949932446103524 1109 10-11 3.9174581348890705 1109 12-13 4.882188332958329 1109 14-15 6.96219883828493 1109 16-17 6.859185712582438 1109 18-19 6.614991064788121 1109 20-21 9.320179085699849 1109 22-23 10.438685316794388 1109 24-25 10.056261860065952 1109 26-27 10.406591040166177 1109 28-29 10.726803222966513 1109 30-31 8.4274268783034 1109 32-33 7.527685095828435 1109 34-35 9.305239946898748 1109 36-37 7.815444470498644 1109 38-39 8.311668775640221 1109 40-41 9.083330397998242 1109 42-43 7.636241052913757 1109 44-45 8.240546379833816 1109 46-47 7.870759738008061 1109 48-49 8.845749248270106 1109 50-51 10.874903359963774 1109 52-53 10.791886927882697 1109 54-55 10.66205187125794 1109 56-57 9.661024104174672 1109 58-59 9.720201196620966 1109 60-61 9.943118705989132 1109 62-63 10.468053470609863 1109 64-65 10.446789813020413 1109 66-67 10.162482381081123 1109 68-69 9.741550607447309 1109 70-71 9.250553233301034 1109 72-73 8.74807251630795 1109 74-75 7.936970045165175 1109 76-77 7.823219967539444 1109 78-79 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2314 48-49 -19.979896705178536 2314 50-51 -17.245425489064626 2314 52-53 -19.47140583316791 2314 54-55 -20.440232988874453 2314 56-57 -20.517600708979288 2314 58-59 -20.276702497592144 2314 60-61 -20.04107945758123 2314 62-63 -19.636046465328636 2314 64-65 -19.350989388346232 2314 66-67 -19.093977153405316 2314 68-69 -18.705491886680385 2314 70-71 -18.417608201676543 2314 72-73 -18.059422679079624 2314 74-75 -17.662538816675525 2314 76-77 -17.312910717918598 2314 78-79 -17.01298510505605 2314 80-81 -16.663191547043873 2314 82-83 -16.25875725881357 2314 84-85 -15.922260023441613 2314 86-87 -15.49329938816107 2314 88-89 -15.134185123238918 2314 90-91 -14.727093513108592 2314 92-93 -14.21797528548835 2314 94-95 -13.70969883045668 2314 96-97 -13.093978121759077 2314 98-99 -12.415105296844636 2314 100-101 -11.231638656143458 2315 1 -18.622779296283774 2315 2 -17.969835068385184 2315 3 -18.732348038223588 2315 4 -20.288864270914104 2315 5 -19.433380164773293 2315 6 -20.088722897561667 2315 7 -19.82734599234448 2315 8 -19.585622043208698 2315 9 -18.550814938051932 2315 10-11 -18.4965926872335 2315 12-13 -18.127634430227662 2315 14-15 -19.718988434830596 2315 16-17 -20.94297102455188 2315 18-19 -21.043027299508267 2315 20-21 -21.29342330592945 2315 22-23 -21.261827175197926 2315 24-25 -21.32618528919734 2315 26-27 -21.18042582936191 2315 28-29 -21.137279629905574 2315 30-31 -21.31985050798744 2315 32-33 -21.291447938551233 2315 34-35 -21.075071819063243 2315 36-37 -20.59871312078899 2315 38-39 -20.632490951030718 2315 40-41 -20.643767616095285 2315 42-43 -20.602174492676678 2315 44-45 -20.303656054503147 2315 46-47 -20.046921235726725 2315 48-49 -19.979896705178536 2315 50-51 -17.245425489064626 2315 52-53 -19.47140583316791 2315 54-55 -20.440232988874453 2315 56-57 -20.517600708979288 2315 58-59 -20.276702497592144 2315 60-61 -20.04107945758123 2315 62-63 -19.636046465328636 2315 64-65 -19.350989388346232 2315 66-67 -19.093977153405316 2315 68-69 -18.705491886680385 2315 70-71 -18.417608201676543 2315 72-73 -18.059422679079624 2315 74-75 -17.662538816675525 2315 76-77 -17.312910717918598 2315 78-79 -17.01298510505605 2315 80-81 -16.663191547043873 2315 82-83 -16.25875725881357 2315 84-85 -15.922260023441613 2315 86-87 -15.49329938816107 2315 88-89 -15.134185123238918 2315 90-91 -14.727093513108592 2315 92-93 -14.21797528548835 2315 94-95 -13.70969883045668 2315 96-97 -13.093978121759077 2315 98-99 -12.415105296844636 2315 100-101 -11.231638656143458 2316 1 -18.622779296283774 2316 2 -17.969835068385184 2316 3 -18.732348038223588 2316 4 -20.288864270914104 2316 5 -19.433380164773293 2316 6 -20.088722897561667 2316 7 -19.82734599234448 2316 8 -19.585622043208698 2316 9 -18.550814938051932 2316 10-11 -18.4965926872335 2316 12-13 -18.127634430227662 2316 14-15 -19.718988434830596 2316 16-17 -20.94297102455188 2316 18-19 -21.043027299508267 2316 20-21 -21.29342330592945 2316 22-23 -21.261827175197926 2316 24-25 -21.32618528919734 2316 26-27 -21.18042582936191 2316 28-29 -21.137279629905574 2316 30-31 -21.31985050798744 2316 32-33 -21.291447938551233 2316 34-35 -21.075071819063243 2316 36-37 -20.59871312078899 2316 38-39 -20.632490951030718 2316 40-41 -20.643767616095285 2316 42-43 -20.602174492676678 2316 44-45 -20.303656054503147 2316 46-47 -20.046921235726725 2316 48-49 -19.979896705178536 2316 50-51 -17.245425489064626 2316 52-53 -19.47140583316791 2316 54-55 -20.440232988874453 2316 56-57 -20.517600708979288 2316 58-59 -20.276702497592144 2316 60-61 -20.04107945758123 2316 62-63 -19.636046465328636 2316 64-65 -19.350989388346232 2316 66-67 -19.093977153405316 2316 68-69 -18.705491886680385 2316 70-71 -18.417608201676543 2316 72-73 -18.059422679079624 2316 74-75 -17.662538816675525 2316 76-77 -17.312910717918598 2316 78-79 -17.01298510505605 2316 80-81 -16.663191547043873 2316 82-83 -16.25875725881357 2316 84-85 -15.922260023441613 2316 86-87 -15.49329938816107 2316 88-89 -15.134185123238918 2316 90-91 -14.727093513108592 2316 92-93 -14.21797528548835 2316 94-95 -13.70969883045668 2316 96-97 -13.093978121759077 2316 98-99 -12.415105296844636 2316 100-101 -11.231638656143458 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1197898.0 3 18288.0 4 59381.0 5 30661.0 6 27039.0 7 27592.0 8 21743.0 9 21701.0 10 24342.0 11 27149.0 12 30419.0 13 31925.0 14 34249.0 15 37049.0 16 39531.0 17 40047.0 18 41066.0 19 44080.0 20 48013.0 21 53235.0 22 58600.0 23 64738.0 24 72187.0 25 81466.0 26 90773.0 27 102427.0 28 116314.0 29 132605.0 30 152231.0 31 175408.0 32 204218.0 33 236248.0 34 271985.0 35 296450.0 36 266326.0 37 143004.0 38 22038.0 39 140.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78216029228257 20.902361440873957 14.806577187011216 23.508901079832253 2 14.23812054451222 20.801339788702585 41.98396447723362 22.976575189551575 3 18.287074235102214 26.39535137732923 30.02982594896462 25.28774843860394 4 11.881953093940293 17.035807852856216 40.76931782343516 30.312921229768335 5 12.615842554142922 37.829231118461585 36.54492296506418 13.010003362331307 6 28.97735080083282 39.00977881994472 18.84956614575056 13.163304233471901 7 25.287185254115528 34.16045030599347 23.506088042460192 17.046276397430805 8 22.99961584514931 39.032004649022426 21.039850162329902 16.928529343498365 9 24.00242805997329 20.79104710347542 21.17560311667199 34.030921719879295 10-11 22.669364568622886 30.43697102838532 28.338323334940284 18.555341068051508 12-13 23.261366393187657 27.55172716156171 28.725796371301794 20.461110073948838 14-15 20.9002034702544 28.23250546687351 26.574118899209427 24.293172163662664 16-17 20.95804171841053 30.716155024041726 27.35755617030516 20.968247087242588 18-19 20.92715537019274 29.553623953368373 29.059767406951654 20.45945326948723 20-21 21.507122758407398 28.606165980631843 29.181502870268357 20.705208390692405 22-23 21.252599156413936 28.25733881595897 29.006173688016588 21.483888339610505 24-25 21.279574330959008 28.32956746185749 29.08906217454862 21.30179603263488 26-27 21.19766076206851 28.516957053431973 28.92208594156748 21.36329624293204 28-29 21.203342651164167 28.586127430949702 28.973307999238802 21.237221918647332 30-31 21.55122044210744 28.357959284176747 28.836731613938376 21.254088659777437 32-33 21.327231279045044 28.437086588720696 28.830326257150258 21.405355875084002 34-35 21.386665852662748 28.432602383250856 28.91981425448052 21.260917509605882 36-37 21.506019673706703 28.29678877636994 28.79826139631767 21.398930153605686 38-39 21.445080235622537 28.380832989575172 28.86081543840589 21.313271336396404 40-41 21.52760917041918 28.352491885951363 28.84106249850929 21.278836445120163 42-43 21.47689102079287 28.363649243022515 28.654620728652112 21.5048390075325 44-45 21.41348341049934 28.535586112490375 28.633022956418408 21.417907520591875 46-47 21.52952798025989 28.381878699800428 28.562371891007167 21.526221428932516 48-49 21.468813120731543 28.444788831579004 28.496476779189532 21.589921268499914 50-51 21.71900255061523 28.539780029169222 28.211006144737183 21.530211275478365 52-53 21.400644589624772 28.509091575661227 28.41873904409032 21.67152479062368 54-55 21.27879318602778 28.488328473698555 28.537770105863615 21.695108234410053 56-57 21.3218339450177 28.54301212920638 28.526452590256046 21.60870133551987 58-59 21.347243995093805 28.411740631942887 28.564730607133786 21.676284765829518 60-61 21.386472862747826 28.52060824773429 28.56391217156191 21.52900671795597 62-63 21.32311194139423 28.40809240164173 28.46756549578665 21.801230161177386 64-65 21.145863674731586 28.387683559419035 28.55315708893269 21.91329567691669 66-67 21.158794519432718 28.425481758529752 28.419151396226503 21.99657232581103 68-69 21.107445784411073 28.396628438454602 28.461315727637253 22.03461004949707 70-71 21.149824717038122 28.161485077164933 28.58029422327292 22.108395982524026 72-73 21.15742175739953 28.29087496956414 28.392439116782153 22.159264156254178 74-75 21.031646828866652 28.362396728096094 28.301318015826283 22.304638427210975 76-77 21.036880008401663 28.28496868845514 28.46077531326295 22.217375989880242 78-79 20.887913698467692 28.37180815444402 28.422742616581502 22.31753553050678 80-81 21.012335930965257 28.387614198522183 28.28213821226776 22.317911658244796 82-83 21.111892570315636 28.153349794789744 28.492796344325207 22.241961290569414 84-85 21.1420298388852 28.19060464124018 28.305308763831988 22.36205675604263 86-87 20.981200129062845 28.283659139808748 28.44428403618392 22.290856694944488 88-89 21.097742160836326 28.088593154081337 28.386560158615325 22.427104526467012 90-91 21.020357118104577 28.01796536076589 28.515438578959422 22.446238942170112 92-93 21.00095526206423 27.880585356935626 28.455075655554268 22.663383725445875 94-95 20.92631255956805 27.910810574147337 28.50988874841423 22.65298811787038 96-97 20.915650685655123 27.746146848350666 28.4477919295254 22.89041053646881 98-99 20.764410507296844 27.666156220805643 28.272952457685584 23.29648081421192 100-101 20.961126876330415 27.642259598896164 28.27161262034345 23.12500090442997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 262090.0 1 238371.5 2 159275.0 3 81272.5 4 49564.0 5 37598.5 6 35206.5 7 36909.5 8 38560.0 9 37784.0 10 33994.0 11 27927.0 12 21220.0 13 15502.5 14 11288.5 15 8950.0 16 8455.5 17 9251.5 18 10765.5 19 12730.0 20 14929.0 21 17044.0 22 19003.5 23 20817.0 24 22592.5 25 24652.5 26 27259.5 27 30664.5 28 35909.5 29 44134.0 30 55468.0 31 69085.0 32 84013.5 33 98221.5 34 110125.0 35 119060.5 36 127059.0 37 133202.0 38 133327.5 39 129397.5 40 125525.5 41 123141.0 42 122387.5 43 123661.0 44 124679.5 45 125570.0 46 126195.5 47 125268.0 48 122894.5 49 118404.5 50 111842.0 51 105127.5 52 98009.5 53 89248.5 54 79571.5 55 68223.5 56 56548.5 57 45436.0 58 35578.0 59 27463.0 60 20680.0 61 15397.5 62 10944.5 63 7234.0 64 4664.5 65 2949.5 66 1746.5 67 1003.0 68 592.5 69 337.5 70 197.5 71 131.5 72 90.5 73 61.0 74 37.0 75 26.0 76 20.0 77 12.0 78 6.5 79 4.0 80 3.0 81 2.0 82 2.5 83 2.0 84 2.0 85 3.5 86 2.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.234780081638366 2 29.283009170154234 3 23.047318106391472 4 29.392644809543484 5 32.539539986266185 6 25.089198414025255 7 24.029018787509504 8 20.03442204447785 9 11.8362046771425 10-11 15.476091785363769 12-13 15.567857805730528 14-15 19.730235533553202 16-17 23.055205148292508 18-19 27.054154156781955 20-21 29.115723284343865 22-23 28.717824898919208 24-25 27.253287572370805 26-27 28.678976899832957 28-29 25.640427802363856 30-31 27.521539108444177 32-33 26.516799053831306 34-35 24.56101991311128 36-37 27.54438274513272 38-39 27.68028165835591 40-41 24.693050145927543 42-43 25.555742848813352 44-45 24.266044546012658 46-47 27.571141117947317 48-49 22.925558298941226 50-51 28.52251410801816 52-53 22.11270939808399 54-55 23.26629002299562 56-57 25.39377639856251 58-59 24.657840548652572 60-61 24.195752004690316 62-63 24.012404186833315 64-65 22.56252409289807 66-67 23.062977050895714 68-69 24.050089279011534 70-71 22.541096669572784 72-73 22.400914574470484 74-75 19.676016438207274 76-77 21.940069534924742 78-79 21.06200573577926 80-81 22.102266263771238 82-83 22.958154234155568 84-85 19.90728523181916 86-87 21.45792372528132 88-89 20.278459786218566 90-91 21.42101006639853 92-93 21.401321246470406 94-95 20.448935030578692 96-97 19.351876286969503 98-99 18.74356083476912 100-101 20.43379421291467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4342566.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.04291141284435 #Duplication Level Percentage of deduplicated Percentage of total 1 83.85094406192378 57.893133027507716 2 10.028776833747495 13.848319010232279 3 2.808942913642892 5.818127902511494 4 1.1749535091028087 3.2448884417278343 5 0.6381588598095479 2.203017281257619 6 0.3666335269854054 1.5188067674779215 7 0.2397050389194847 1.1584953637131274 8 0.16472667202642047 0.9098567219242251 9 0.12951535550678392 0.8047905495172134 >10 0.5750837430901132 6.634249504237162 >50 0.016668272273235564 0.7866541958937737 >100 0.005756953656056785 0.638318667283789 >500 3.356482902136262E-5 0.01872470121501039 >1k 6.712965796203776E-5 0.06936018647172745 >5k 0.0 0.0 >10k+ 3.356482898100031E-5 4.453257679029125 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 192165 4.425148633319562 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3027859565059E-5 0.0 5 0.0 0.0 0.0 2.3027859565059E-5 0.0 6 0.0 0.0 0.0 2.3027859565059E-5 0.0 7 0.0 2.3027859565059E-5 0.0 2.3027859565059E-5 0.0 8 0.0 2.3027859565059E-5 0.0 2.3027859565059E-5 0.0 9 0.0 2.3027859565059E-5 0.0 2.3027859565059E-5 0.0 10-11 0.0 2.3027859565059E-5 0.0 6.9083578695177E-5 0.0 12-13 0.0 3.45417893475885E-5 0.0 6.9083578695177E-5 0.0 14-15 0.0 4.6055719130118E-5 0.0 6.9083578695177E-5 0.0 16-17 0.0 4.6055719130118E-5 0.0 6.9083578695177E-5 0.0 18-19 0.0 6.9083578695177E-5 0.0 8.05975084777065E-5 0.0 20-21 0.0 9.2111438260236E-5 0.0 1.0362536804276549E-4 0.0 22-23 0.0 1.0362536804276549E-4 0.0 1.38167157390354E-4 0.0 24-25 0.0 1.15139297825295E-4 0.0 1.496810871728835E-4 0.0 26-27 0.0 1.15139297825295E-4 0.0 2.3027859565059E-4 0.0 28-29 0.0 1.15139297825295E-4 0.0 5.29640769996357E-4 0.0 30-31 0.0 1.15139297825295E-4 0.0 0.0023373277458534885 0.0 32-33 0.0 1.15139297825295E-4 0.0 0.008140348356248356 0.0 34-35 0.0 1.15139297825295E-4 0.0 0.016741253903797892 0.0 36-37 0.0 1.15139297825295E-4 0.0 0.03158270939347842 0.0 38-39 0.0 1.15139297825295E-4 0.0 0.056072838040918664 0.0 40-41 0.0 1.15139297825295E-4 0.0 0.08043631346075109 0.0 42-43 0.0 1.15139297825295E-4 0.0 0.10751707630926047 0.0 44-45 0.0 1.15139297825295E-4 0.0 0.1411722930635942 0.0 46-47 0.0 1.15139297825295E-4 0.0 0.18279514922743834 0.0 48-49 0.0 1.15139297825295E-4 0.0 0.22886238228733885 0.0 50-51 0.0 1.266532276078245E-4 0.0 0.2823906418463185 0.0 52-53 0.0 1.38167157390354E-4 0.0 0.34367929007872305 0.0 54-55 0.0 1.38167157390354E-4 0.0 0.4124519926697717 0.0 56-57 0.0 1.38167157390354E-4 0.0 0.4933373493920415 0.0 58-59 0.0 1.38167157390354E-4 0.0 0.5838368374827234 0.0 60-61 0.0 1.38167157390354E-4 0.0 0.6823730485616109 0.0 62-63 0.0 1.38167157390354E-4 0.0 0.7851578997302516 0.0 64-65 0.0 1.61195016955413E-4 0.0 0.8960370435360108 0.0 66-67 0.0 1.61195016955413E-4 0.0 1.0253960446427297 0.0 68-69 0.0 1.61195016955413E-4 0.0 1.1686408450671792 0.0 70-71 2.3027859565059E-5 1.61195016955413E-4 0.0 1.3292831933930307 0.0 72-73 2.3027859565059E-5 1.61195016955413E-4 0.0 1.4963963702566638 0.0 74-75 2.3027859565059E-5 1.61195016955413E-4 0.0 1.6731236784887091 0.0 76-77 2.3027859565059E-5 1.61195016955413E-4 0.0 1.8616067090287172 0.0 78-79 2.3027859565059E-5 1.61195016955413E-4 0.0 2.0786097436400506 0.0 80-81 2.3027859565059E-5 1.61195016955413E-4 0.0 2.315520362845378 0.0 82-83 2.3027859565059E-5 1.61195016955413E-4 0.0 2.5670767007340825 0.0 84-85 2.3027859565059E-5 1.61195016955413E-4 0.0 2.819485069426694 0.0 86-87 2.3027859565059E-5 1.61195016955413E-4 0.0 3.0862167667687723 0.0 88-89 2.3027859565059E-5 1.61195016955413E-4 0.0 3.3814339263928286 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4550 0.0 42.581135 1 ATCAACG 4685 0.0 38.607944 3 GGTATCA 2470 0.0 37.974407 1 TCAACGC 4690 0.0 37.59277 4 CAACGCA 4905 0.0 35.939377 5 TATCAAC 5465 0.0 33.571922 2 AACGCAG 5255 0.0 32.58709 6 ACGCAGA 6330 0.0 26.20611 7 GTACATG 5975 0.0 25.477417 1 CGCAGAG 6435 0.0 25.226068 8 TACATGG 6445 0.0 22.423815 2 ACATGGG 6435 0.0 21.531582 3 GCAGAGT 7535 0.0 20.65087 9 GAGTACA 4925 0.0 19.461315 1 GTGGTAT 1820 0.0 17.74214 1 TATACTG 2105 0.0 17.466757 5 GTATAGG 1005 0.0 17.340025 1 AGAGTAC 6785 0.0 16.941769 10-11 AGTACAT 5055 0.0 16.525314 2 CAGAGTA 7345 0.0 15.71244 10-11 >>END_MODULE