FastQCFastQC Report
Sat 18 Jun 2016
SRR1931027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342566
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.11946853542352609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.043.507931
GTATCAA75100.043.4792021
TCAACGC88700.036.003554
CAACGCA89000.035.7673265
ATCAACG89950.035.5572853
TATCAAC95850.033.5196382
AACGCAG98000.032.7726486
ACGCAGA113550.027.908297
CGCAGAG116350.027.1143518
GCAGAGT138000.022.275889
GTACATG99200.021.4650121
ACATGGG99150.019.6519583
TACATGG106700.019.3317012
AGAGTAC125800.018.89278810-11
GTGGTAT26700.017.8013761
TATACCG3301.2314558E-817.2512785
GAGTACT86350.017.00345812-13
CAGAGTA133300.016.86891410-11
TATACAG32850.016.4635475
GAGTACA80800.016.176561
Produced by FastQC (version 0.11.5)