FastQCFastQC Report
Sat 18 Jun 2016
SRR1931026_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931026_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714329
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1698764.573531316154277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23950.050.5950241
GTATCAA47650.047.8433341
ATCAACG57600.038.894313
TCAACGC58800.038.439084
CAACGCA59100.038.4076165
TATCAAC61100.036.212292
AACGCAG64100.034.8792726
ACGCAGA71850.029.9394197
CGCAGAG74100.028.5531868
GTACATG57950.027.0238821
TACATGG60700.025.6255932
GCAGAGT79450.024.529969
ACATGGG61850.024.4741233
AGAGTAC72050.020.59751310-11
GAGTACT51300.019.99052812-13
GGCGTCG3153.8198777E-1119.6726288
CAGAGTA78250.019.22850810-11
GAGTACA43100.018.1078821
CATGGGG40050.017.8878484
AGTACTT59600.017.8110612-13
Produced by FastQC (version 0.11.5)