##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931026_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3714329 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.42737732710269 28.0 2.0 31.0 2.0 33.0 2 19.202521370616335 26.0 2.0 31.0 2.0 33.0 3 19.804760429138074 26.0 2.0 31.0 2.0 33.0 4 21.308332137513936 28.0 2.0 35.0 2.0 37.0 5 20.667206109098036 28.0 2.0 35.0 2.0 37.0 6 21.561676415847923 29.0 2.0 35.0 2.0 37.0 7 21.698461821771847 28.0 2.0 35.0 2.0 37.0 8 22.072080313833265 30.0 2.0 35.0 2.0 37.0 9 23.28336504386122 31.0 2.0 37.0 2.0 39.0 10-11 23.57805609034633 32.0 2.0 37.0 2.0 39.0 12-13 23.46182581026075 32.0 2.0 37.0 2.0 39.0 14-15 23.40714998590593 31.5 2.0 38.0 2.0 40.0 16-17 22.33259075865385 29.5 2.0 37.0 2.0 40.0 18-19 21.339672387664095 27.0 2.0 37.0 2.0 39.5 20-21 21.007294588066916 27.0 2.0 37.0 2.0 39.5 22-23 20.64350667374915 26.0 2.0 37.0 2.0 39.0 24-25 20.46732155390651 25.0 2.0 36.0 2.0 39.0 26-27 20.167064091522317 24.5 2.0 36.0 2.0 39.0 28-29 19.939589761703928 24.0 2.0 36.0 2.0 39.0 30-31 19.884863591781986 24.0 2.0 36.0 2.0 39.0 32-33 19.748430874055583 24.0 2.0 36.0 2.0 39.0 34-35 19.40473609096017 23.5 2.0 35.5 2.0 39.0 36-37 18.974389990762802 19.0 2.0 35.0 2.0 38.5 38-39 18.822031381711206 19.0 2.0 35.0 2.0 38.0 40-41 18.653301713445416 19.0 2.0 35.0 2.0 38.0 42-43 18.491309197435122 18.5 2.0 35.0 2.0 38.0 44-45 18.10177329471891 15.5 2.0 34.0 2.0 38.0 46-47 17.766816429023926 15.0 2.0 34.0 2.0 38.0 48-49 17.491528617954955 14.0 2.0 33.5 2.0 38.0 50-51 15.398029361427056 7.0 2.0 31.0 2.0 37.5 52-53 17.20645599783972 13.5 2.0 33.5 2.0 37.5 54-55 17.90612247864958 14.5 2.0 34.5 2.0 38.0 56-57 17.840647664759906 13.5 2.0 35.0 2.0 39.0 58-59 17.557903729045005 10.5 2.0 34.0 2.0 38.5 60-61 17.254837010937912 8.0 2.0 34.0 2.0 38.0 62-63 16.813958456561064 7.5 2.0 34.0 2.0 38.0 64-65 16.44221580802347 4.0 2.0 33.5 2.0 38.0 66-67 16.06111938387795 2.0 2.0 33.0 2.0 38.0 68-69 15.60597970723649 2.0 2.0 32.0 2.0 37.0 70-71 15.24046267845417 2.0 2.0 31.5 2.0 36.5 72-73 14.824316182007571 2.0 2.0 31.0 2.0 36.0 74-75 14.359067950092737 2.0 2.0 31.0 2.0 35.5 76-77 13.930665673396192 2.0 2.0 30.0 2.0 35.0 78-79 13.535787217556656 2.0 2.0 30.0 2.0 34.5 80-81 13.081341744363517 2.0 2.0 29.0 2.0 34.0 82-83 12.609020633336465 2.0 2.0 29.0 2.0 34.0 84-85 12.187894906455513 2.0 2.0 28.5 2.0 34.0 86-87 11.699080776097109 2.0 2.0 26.5 2.0 34.0 88-89 11.269437360018458 2.0 2.0 26.0 2.0 33.5 90-91 10.771009380159917 2.0 2.0 25.5 2.0 33.0 92-93 10.227418734312442 2.0 2.0 24.5 2.0 32.5 94-95 9.670358495437533 2.0 2.0 21.0 2.0 32.0 96-97 9.046631841175081 2.0 2.0 12.5 2.0 32.0 98-99 8.398365088283779 2.0 2.0 2.0 2.0 32.0 100-101 7.5598470140905665 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.123093521597823 1101 2 9.716262321593899 1101 3 9.031093235879947 1101 4 11.143558086906115 1101 5 11.720963010670296 1101 6 10.961518567327733 1101 7 10.74008452317031 1101 8 10.196975963918963 1101 9 10.14273102084007 1101 10-11 9.778023849865345 1101 12-13 9.580788223802127 1101 14-15 10.27430617002199 1101 16-17 11.162824931372715 1101 18-19 11.990726370833183 1101 20-21 12.216748695017866 1101 22-23 12.404664100129775 1101 24-25 12.161987218925841 1101 26-27 12.257982370112583 1101 28-29 12.22363555943755 1101 30-31 11.73134583902344 1101 32-33 11.636552382462359 1101 34-35 11.54870005068435 1101 36-37 11.955501924851148 1101 38-39 11.69216038665073 1101 40-41 11.473947475413464 1101 42-43 11.111036487547711 1101 44-45 11.129210203973766 1101 46-47 10.99331744639419 1101 48-49 11.127252162912907 1101 50-51 12.466457211751608 1101 52-53 11.878986613679185 1101 54-55 11.695311343112497 1101 56-57 11.689077286608335 1101 58-59 11.55875450504157 1101 60-61 11.171346224940294 1101 62-63 11.175868654941489 1101 64-65 10.828695176530815 1101 66-67 10.502222224328982 1101 68-69 10.525240409400181 1101 70-71 10.523914304874557 1101 72-73 10.090058448869422 1101 74-75 9.867882328995316 1101 76-77 9.73313101021907 1101 78-79 9.37011282489221 1101 80-81 9.205691315225641 1101 82-83 8.990932179991281 1101 84-85 8.701280284887083 1101 86-87 8.367290623712163 1101 88-89 8.172769499316297 1101 90-91 7.708084936508824 1101 92-93 7.624190308993571 1101 94-95 7.231965089011501 1101 96-97 7.060822486346263 1101 98-99 6.379599918041176 1101 100-101 5.472599849720765 1102 1 8.04499366849846 1102 2 8.98903396296269 1102 3 8.656282081113847 1102 4 10.513084147018883 1102 5 11.347100280315424 1102 6 10.502432740137952 1102 7 10.144869308439699 1102 8 9.620051315928293 1102 9 9.570587829783012 1102 10-11 8.939689515509816 1102 12-13 9.043584691530132 1102 14-15 9.520080469520767 1102 16-17 10.272285952778457 1102 18-19 11.226977166986234 1102 20-21 11.251585856189504 1102 22-23 11.570279290049154 1102 24-25 11.239486534347687 1102 26-27 11.523182350142566 1102 28-29 11.217405368708782 1102 30-31 11.273027117588505 1102 32-33 10.99009743829519 1102 34-35 10.807431334468848 1102 36-37 10.8411925015761 1102 38-39 10.654500040292003 1102 40-41 10.342012698691846 1102 42-43 10.137298872360816 1102 44-45 10.176347759553192 1102 46-47 10.243972977256188 1102 48-49 9.659384219173884 1102 50-51 11.66003064921142 1102 52-53 10.988266543360528 1102 54-55 10.457832617986135 1102 56-57 10.453690798743903 1102 58-59 10.560751128293628 1102 60-61 10.182633519321008 1102 62-63 10.112036696563838 1102 64-65 9.886890522609658 1102 66-67 9.722918118826787 1102 68-69 9.566295128753397 1102 70-71 9.206330328693339 1102 72-73 9.011857535392535 1102 74-75 8.672482240340555 1102 76-77 8.463279605283146 1102 78-79 7.878301060435511 1102 80-81 7.64099875511514 1102 82-83 7.49205254320349 1102 84-85 7.379350561184339 1102 86-87 7.006311276035719 1102 88-89 6.778867918205515 1102 90-91 6.690078942478648 1102 92-93 6.4872415456972465 1102 94-95 6.188056397365171 1102 96-97 5.926582534516362 1102 98-99 5.454086408780082 1102 100-101 4.794968308595393 1103 1 8.058578256226255 1103 2 9.427030496151016 1103 3 8.764871198462885 1103 4 10.711495122857364 1103 5 11.335205306189703 1103 6 10.534775150014411 1103 7 10.302575474032743 1103 8 9.815737294037483 1103 9 9.875817360082518 1103 10-11 9.25986701709341 1103 12-13 9.011297353708859 1103 14-15 9.680251902119654 1103 16-17 10.586496577807207 1103 18-19 11.103790932316468 1103 20-21 11.513480535418008 1103 22-23 11.749750418432068 1103 24-25 11.733274155860084 1103 26-27 11.628357737313124 1103 28-29 11.294024089020002 1103 30-31 11.101688001124156 1103 32-33 11.418711521642582 1103 34-35 11.058209273326042 1103 36-37 11.344673173422734 1103 38-39 11.084923170614164 1103 40-41 10.931811923819918 1103 42-43 10.683610047313898 1103 44-45 10.56364733265998 1103 46-47 10.321059035931295 1103 48-49 10.3424496285561 1103 50-51 12.131826237077226 1103 52-53 11.347784439493008 1103 54-55 10.925775152545139 1103 56-57 10.665819582127742 1103 58-59 10.650642140180341 1103 60-61 10.557516076504527 1103 62-63 10.678673588441544 1103 64-65 10.210748194006868 1103 66-67 10.019675841889992 1103 68-69 9.79986131784385 1103 70-71 9.463261076800391 1103 72-73 9.320106297119123 1103 74-75 9.272942442191804 1103 76-77 8.933728028939568 1103 78-79 8.396610270193541 1103 80-81 8.164462547045103 1103 82-83 7.822053734588243 1103 84-85 7.497403888627588 1103 86-87 7.145009104294656 1103 88-89 6.8426692258977315 1103 90-91 6.692113806996966 1103 92-93 6.326800798215375 1103 94-95 6.027977709940886 1103 96-97 5.860097957050991 1103 98-99 5.324816813154943 1103 100-101 4.664477912218145 1104 1 7.884333536393168 1104 2 9.418380365225467 1104 3 8.751460001647985 1104 4 10.747829471219664 1104 5 11.412365090401924 1104 6 10.639011108987443 1104 7 10.378970474723637 1104 8 9.886590248046982 1104 9 9.749622990540498 1104 10-11 9.111800921048467 1104 12-13 9.047837213902046 1104 14-15 9.680470027754286 1104 16-17 10.349095187651361 1104 18-19 11.277095422085235 1104 20-21 11.357212277653776 1104 22-23 11.480725971447022 1104 24-25 11.32880784446311 1104 26-27 11.485798247461215 1104 28-29 11.205191232813352 1104 30-31 11.105227749799852 1104 32-33 11.10171581148256 1104 34-35 10.994039700228761 1104 36-37 10.90489863943111 1104 38-39 10.667167464444692 1104 40-41 10.457101847798818 1104 42-43 10.28465525145971 1104 44-45 10.259210527530062 1104 46-47 10.119953041851758 1104 48-49 10.024644556545613 1104 50-51 11.497454897588737 1104 52-53 10.709234347498242 1104 54-55 10.428139989904473 1104 56-57 10.349313982745588 1104 58-59 10.306939280688368 1104 60-61 10.198737177276776 1104 62-63 10.04442523353956 1104 64-65 9.74590884943158 1104 66-67 9.381194337453085 1104 68-69 9.174026190865847 1104 70-71 9.052276367310627 1104 72-73 8.901858473430286 1104 74-75 8.59211629108187 1104 76-77 8.198971774929666 1104 78-79 7.932931610570726 1104 80-81 7.808446250010666 1104 82-83 7.346329429909842 1104 84-85 6.977883561482855 1104 86-87 6.6754357490056595 1104 88-89 6.1034100231245265 1104 90-91 5.7429307729643515 1104 92-93 5.4504931194476764 1104 94-95 5.080987471018242 1104 96-97 4.794475503104275 1104 98-99 4.299311666149659 1104 100-101 3.7712515391626065 1105 1 8.109063652245435 1105 2 9.120081441400355 1105 3 8.80109690323225 1105 4 10.887547035564634 1105 5 10.71225790493314 1105 6 10.702187330038438 1105 7 10.210560307773516 1105 8 9.854927957842463 1105 9 9.776209598719124 1105 10-11 9.19487250707807 1105 12-13 8.948111317539944 1105 14-15 9.766423681846664 1105 16-17 10.561163533807086 1105 18-19 11.063935565015864 1105 20-21 11.412435714765035 1105 22-23 11.787510168452695 1105 24-25 11.73478503185029 1105 26-27 11.874787975798284 1105 28-29 11.519972700729184 1105 30-31 11.175892645600324 1105 32-33 11.11931705875691 1105 34-35 11.14919354874413 1105 36-37 11.083267115734664 1105 38-39 11.08742573336048 1105 40-41 10.962783504178265 1105 42-43 10.720587693497809 1105 44-45 10.533554276996114 1105 46-47 10.24395604404506 1105 48-49 10.332005660305896 1105 50-51 11.761367129692788 1105 52-53 10.825011740115865 1105 54-55 10.702359689832392 1105 56-57 10.513731678516386 1105 58-59 10.428758462303147 1105 60-61 10.185881623587292 1105 62-63 10.130586493493908 1105 64-65 9.894546763276743 1105 66-67 9.719083782504349 1105 68-69 9.41526267443234 1105 70-71 9.25711674417118 1105 72-73 9.111559425138898 1105 74-75 8.709640135889648 1105 76-77 8.552951889774812 1105 78-79 8.279367381178822 1105 80-81 7.954656411598732 1105 82-83 7.715239767931633 1105 84-85 7.445553272155713 1105 86-87 7.17769923633684 1105 88-89 6.991223746559628 1105 90-91 6.625421583505146 1105 92-93 6.160370768818668 1105 94-95 5.8941624418499305 1105 96-97 5.560921200453684 1105 98-99 5.023911898107016 1105 100-101 4.534864968851731 1106 1 7.842541872813548 1106 2 9.153795949147526 1106 3 8.708581674848254 1106 4 10.394716757608563 1106 5 10.900756951862377 1106 6 10.199154183007941 1106 7 9.622334167281672 1106 8 9.125324669168997 1106 9 8.945830461403101 1106 10-11 8.71674874152971 1106 12-13 8.489152034706159 1106 14-15 8.958092625428211 1106 16-17 9.967976615531892 1106 18-19 10.961237609496816 1106 20-21 11.06807261501353 1106 22-23 11.032878937048618 1106 24-25 10.603180294573928 1106 26-27 10.94633140343631 1106 28-29 10.739710885157674 1106 30-31 10.570228705303457 1106 32-33 10.657035164299057 1106 34-35 10.618289616229191 1106 36-37 10.575616479626618 1106 38-39 10.374620283207669 1106 40-41 10.263717281792932 1106 42-43 10.082949670689857 1106 44-45 9.778180623137835 1106 46-47 9.51769631730718 1106 48-49 9.694574311310621 1106 50-51 11.23556550745156 1106 52-53 10.676960402017446 1106 54-55 10.103745832501453 1106 56-57 10.0576456871207 1106 58-59 9.716642823050531 1106 60-61 9.481125673833546 1106 62-63 9.721513405334463 1106 64-65 9.58291620120481 1106 66-67 9.235296831139788 1106 68-69 8.796351737642192 1106 70-71 8.665001255814703 1106 72-73 8.318679526605768 1106 74-75 8.084460677272643 1106 76-77 7.773619297988196 1106 78-79 7.63774421840662 1106 80-81 7.376660288012642 1106 82-83 7.285638069056729 1106 84-85 6.937904791706735 1106 86-87 6.564122073712303 1106 88-89 6.302769699013334 1106 90-91 6.040063277939398 1106 92-93 5.692365734276928 1106 94-95 5.473109952090658 1106 96-97 4.913320125642075 1106 98-99 4.487680658403656 1106 100-101 3.9424249864419094 1107 1 7.257755217007066 1107 2 7.895917185165015 1107 3 8.199071328898647 1107 4 9.578134445997954 1107 5 10.539939454041441 1107 6 9.759699163216226 1107 7 9.533366349353408 1107 8 8.10479899993286 1107 9 5.442505665313192 1107 10-11 7.625678651424938 1107 12-13 7.755651604656496 1107 14-15 8.365666660645914 1107 16-17 9.0347020696253 1107 18-19 10.163102666645084 1107 20-21 10.581691323785154 1107 22-23 9.948568014677889 1107 24-25 10.240686611706149 1107 26-27 10.373788227524123 1107 28-29 10.329409184948528 1107 30-31 10.076892706701411 1107 32-33 10.393767811212168 1107 34-35 10.406470834939903 1107 36-37 10.690943309512178 1107 38-39 10.30137455966712 1107 40-41 10.130505828612801 1107 42-43 9.779585720824507 1107 44-45 9.36920732184711 1107 46-47 9.556638473727887 1107 48-49 9.752608288770936 1107 50-51 11.206658192520282 1107 52-53 10.149793982621699 1107 54-55 10.006600717789667 1107 56-57 9.69561414717612 1107 58-59 9.881742072435063 1107 60-61 9.473462720640711 1107 62-63 9.84922885523872 1107 64-65 9.733797351091855 1107 66-67 9.591597923279927 1107 68-69 9.23538905384934 1107 70-71 9.0927013664513 1107 72-73 8.511646564078381 1107 74-75 8.535616145924605 1107 76-77 8.280577509421727 1107 78-79 7.926346251783087 1107 80-81 7.5595930734638355 1107 82-83 7.384311674329558 1107 84-85 7.0425981425861295 1107 86-87 6.614448593144706 1107 88-89 6.469341635986888 1107 90-91 6.196020664379693 1107 92-93 5.963360489059541 1107 94-95 5.7216806886453515 1107 96-97 5.198665020757376 1107 98-99 4.864547077878003 1107 100-101 4.474357418474283 1108 1 6.779477634200372 1108 2 7.843229772069758 1108 3 7.599100103115717 1108 4 8.863935030539182 1108 5 10.621180370695889 1108 6 9.165942039416663 1108 7 8.505215979887584 1108 8 5.626664326077645 1108 9 6.949050805491606 1108 10-11 6.138942629017226 1108 12-13 4.858925451176642 1108 14-15 7.171466031615616 1108 16-17 8.306746673382012 1108 18-19 7.830273812173221 1108 20-21 8.031366366276192 1108 22-23 8.741846913042298 1108 24-25 8.35766823610371 1108 26-27 6.751823230724181 1108 28-29 8.705247893082397 1108 30-31 9.030371769962741 1108 32-33 9.410878335891994 1108 34-35 9.208038709058926 1108 36-37 8.987863563879849 1108 38-39 8.679530246526152 1108 40-41 8.986973221959943 1108 42-43 8.418823522217753 1108 44-45 7.927107287633628 1108 46-47 8.636692854817316 1108 48-49 9.037584517008593 1108 50-51 10.461716290653575 1108 52-53 9.852847045074576 1108 54-55 9.274967436527017 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48-49 -15.398371105785156 2313 50-51 -13.363044173289945 2313 52-53 -15.1271075691463 2313 54-55 -15.797901953286395 2313 56-57 -15.743332685690834 2313 58-59 -15.464098680553999 2313 60-61 -15.149152390018152 2313 62-63 -14.71360502926904 2313 64-65 -14.361471028455334 2313 66-67 -13.974924590075453 2313 68-69 -13.525867623840815 2313 70-71 -13.158634171579736 2313 72-73 -12.744013573291243 2313 74-75 -12.277408529730446 2313 76-77 -11.857173698922205 2313 78-79 -11.44747720280862 2313 80-81 -11.004003975633085 2313 82-83 -10.535937626102902 2313 84-85 -10.11423218048688 2313 86-87 -9.630908822271218 2313 88-89 -9.201607231985639 2313 90-91 -8.713798720000872 2313 92-93 -8.174009136989808 2313 94-95 -7.619835465005119 2313 96-97 -7.008152582905813 2313 98-99 -6.374832214922812 2313 100-101 -5.546375219531314 2314 1 -18.28078459474567 2314 2 -17.074319395857625 2314 3 -17.671953855532795 2314 4 -19.17943360284458 2314 5 -18.51144696162887 2314 6 -19.407179493614098 2314 7 -19.542186944973736 2314 8 -19.931832900346965 2314 9 -21.130122164755495 2314 10-11 -21.442751773403348 2314 12-13 -21.327660529660474 2314 14-15 -21.282896046044495 2314 16-17 -20.195900830400138 2314 18-19 -19.20830410008094 2314 20-21 -18.877988147087397 2314 22-23 -18.518743102086294 2314 24-25 -18.332195606558923 2314 26-27 -18.04658826700653 2314 28-29 -17.81126748765386 2314 30-31 -17.76602258202919 2314 32-33 -17.638793877925455 2314 34-35 -17.278723772030332 2314 36-37 -16.863750152711177 2314 38-39 -16.719612466534866 2314 40-41 -16.548074685251354 2314 42-43 -16.385639412729297 2314 44-45 -16.012756555029004 2314 46-47 -15.672056514824643 2314 48-49 -15.398371105785156 2314 50-51 -13.363044173289945 2314 52-53 -15.1271075691463 2314 54-55 -15.797901953286395 2314 56-57 -15.743332685690834 2314 58-59 -15.464098680553999 2314 60-61 -15.149152390018152 2314 62-63 -14.71360502926904 2314 64-65 -14.361471028455334 2314 66-67 -13.974924590075453 2314 68-69 -13.525867623840815 2314 70-71 -13.158634171579736 2314 72-73 -12.744013573291243 2314 74-75 -12.277408529730446 2314 76-77 -11.857173698922205 2314 78-79 -11.44747720280862 2314 80-81 -11.004003975633085 2314 82-83 -10.535937626102902 2314 84-85 -10.11423218048688 2314 86-87 -9.630908822271218 2314 88-89 -9.201607231985639 2314 90-91 -8.713798720000872 2314 92-93 -8.174009136989808 2314 94-95 -7.619835465005119 2314 96-97 -7.008152582905813 2314 98-99 -6.374832214922812 2314 100-101 -5.546375219531314 2315 1 -18.28078459474567 2315 2 -17.074319395857625 2315 3 -17.671953855532795 2315 4 -19.17943360284458 2315 5 -18.51144696162887 2315 6 -19.407179493614098 2315 7 -19.542186944973736 2315 8 -19.931832900346965 2315 9 -21.130122164755495 2315 10-11 -21.442751773403348 2315 12-13 -21.327660529660474 2315 14-15 -21.282896046044495 2315 16-17 -20.195900830400138 2315 18-19 -19.20830410008094 2315 20-21 -18.877988147087397 2315 22-23 -18.518743102086294 2315 24-25 -18.332195606558923 2315 26-27 -18.04658826700653 2315 28-29 -17.81126748765386 2315 30-31 -17.76602258202919 2315 32-33 -17.638793877925455 2315 34-35 -17.278723772030332 2315 36-37 -16.863750152711177 2315 38-39 -16.719612466534866 2315 40-41 -16.548074685251354 2315 42-43 -16.385639412729297 2315 44-45 -16.012756555029004 2315 46-47 -15.672056514824643 2315 48-49 -15.398371105785156 2315 50-51 -13.363044173289945 2315 52-53 -15.1271075691463 2315 54-55 -15.797901953286395 2315 56-57 -15.743332685690834 2315 58-59 -15.464098680553999 2315 60-61 -15.149152390018152 2315 62-63 -14.71360502926904 2315 64-65 -14.361471028455334 2315 66-67 -13.974924590075453 2315 68-69 -13.525867623840815 2315 70-71 -13.158634171579736 2315 72-73 -12.744013573291243 2315 74-75 -12.277408529730446 2315 76-77 -11.857173698922205 2315 78-79 -11.44747720280862 2315 80-81 -11.004003975633085 2315 82-83 -10.535937626102902 2315 84-85 -10.11423218048688 2315 86-87 -9.630908822271218 2315 88-89 -9.201607231985639 2315 90-91 -8.713798720000872 2315 92-93 -8.174009136989808 2315 94-95 -7.619835465005119 2315 96-97 -7.008152582905813 2315 98-99 -6.374832214922812 2315 100-101 -5.546375219531314 2316 1 -18.28078459474567 2316 2 -17.074319395857625 2316 3 -17.671953855532795 2316 4 -19.17943360284458 2316 5 -18.51144696162887 2316 6 -19.407179493614098 2316 7 -19.542186944973736 2316 8 -19.931832900346965 2316 9 -21.130122164755495 2316 10-11 -21.442751773403348 2316 12-13 -21.327660529660474 2316 14-15 -21.282896046044495 2316 16-17 -20.195900830400138 2316 18-19 -19.20830410008094 2316 20-21 -18.877988147087397 2316 22-23 -18.518743102086294 2316 24-25 -18.332195606558923 2316 26-27 -18.04658826700653 2316 28-29 -17.81126748765386 2316 30-31 -17.76602258202919 2316 32-33 -17.638793877925455 2316 34-35 -17.278723772030332 2316 36-37 -16.863750152711177 2316 38-39 -16.719612466534866 2316 40-41 -16.548074685251354 2316 42-43 -16.385639412729297 2316 44-45 -16.012756555029004 2316 46-47 -15.672056514824643 2316 48-49 -15.398371105785156 2316 50-51 -13.363044173289945 2316 52-53 -15.1271075691463 2316 54-55 -15.797901953286395 2316 56-57 -15.743332685690834 2316 58-59 -15.464098680553999 2316 60-61 -15.149152390018152 2316 62-63 -14.71360502926904 2316 64-65 -14.361471028455334 2316 66-67 -13.974924590075453 2316 68-69 -13.525867623840815 2316 70-71 -13.158634171579736 2316 72-73 -12.744013573291243 2316 74-75 -12.277408529730446 2316 76-77 -11.857173698922205 2316 78-79 -11.44747720280862 2316 80-81 -11.004003975633085 2316 82-83 -10.535937626102902 2316 84-85 -10.11423218048688 2316 86-87 -9.630908822271218 2316 88-89 -9.201607231985639 2316 90-91 -8.713798720000872 2316 92-93 -8.174009136989808 2316 94-95 -7.619835465005119 2316 96-97 -7.008152582905813 2316 98-99 -6.374832214922812 2316 100-101 -5.546375219531314 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1026297.0 3 41706.0 4 139954.0 5 77647.0 6 49801.0 7 48552.0 8 46335.0 9 46587.0 10 49858.0 11 52344.0 12 53645.0 13 54174.0 14 54686.0 15 55967.0 16 57729.0 17 58606.0 18 58892.0 19 60356.0 20 63193.0 21 66386.0 22 70715.0 23 74396.0 24 78615.0 25 83109.0 26 88369.0 27 93999.0 28 100934.0 29 107563.0 30 114906.0 31 122425.0 32 130330.0 33 134741.0 34 132667.0 35 115879.0 36 75337.0 37 25413.0 38 2209.0 39 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.35167188398479 20.976059850200144 14.103008934359432 23.569259331455633 2 14.586717233727697 20.980233667766417 42.005338431017606 22.42771066748828 3 18.340761346027843 26.507214188432894 30.302306833466048 24.849717632073215 4 11.85536893300294 17.46882546339383 40.37825130075124 30.297554302851992 5 12.315768758639935 38.273480863501526 36.37608256269548 13.034667815163056 6 29.17246689519567 39.22071029598566 18.555293578566996 13.05152923025167 7 25.850082620013293 32.91515619235839 23.766351245228414 17.468409942399905 8 24.137964931500903 37.095608573505004 21.208788005336746 17.557638489657343 9 25.30550974217281 16.603240223703587 21.726902616919723 36.36434741720388 10-11 23.551208540092667 27.380559799339277 29.884126152568324 19.18410550799973 12-13 24.257126920574066 24.837827094639863 29.85207612734051 21.05296985744556 14-15 21.473199649781332 26.587360710210252 27.330470188302435 24.608969451705985 16-17 20.937298693550897 29.6161607186616 28.082979069241837 21.36356151854567 18-19 20.77872239054858 28.78711968376151 29.89685771501314 20.537300210676772 20-21 21.57072003300378 28.12655999227049 29.77400768196747 20.52871229275826 22-23 21.14667326506935 28.09293453665221 29.494783741545955 21.265608456732487 24-25 21.046807705208952 28.225777398479334 29.772333202482837 20.955081693828877 26-27 21.08381544182219 28.386154136290813 29.51576432848018 21.014266093406814 28-29 21.041177009694614 28.469254258441023 29.593969775118968 20.89559895674539 30-31 21.34956214556638 28.21556348865859 29.5481223792037 20.886751986571326 32-33 21.080442778250816 28.417774754604793 29.43654381865297 21.06523864849142 34-35 21.269748480332684 28.35406472222397 29.57060798384058 20.805578813602764 36-37 21.289247148517116 28.270491019446148 29.382414652393784 21.057847179642952 38-39 21.287087419715782 28.359763470126794 29.508672235994833 20.84447687416259 40-41 21.480594116292203 28.267363865771273 29.41374503980948 20.83829697812704 42-43 21.282440443197135 28.24772741859258 29.251973557538026 21.217858580672264 44-45 21.351372942663804 28.48872596065147 29.12116551724879 21.038735579435937 46-47 21.370615492761527 28.320050910900484 28.922442815707306 21.386890780630683 48-49 21.434579442542816 28.357604636768404 28.954593497623804 21.25322242306498 50-51 21.599988295428698 28.608689390129133 28.532818686880105 21.25850362756206 52-53 21.032597405559883 28.89140333345 28.690996529753072 21.385002731237044 54-55 21.052303214530436 28.784892684322642 28.770435347869494 21.392368753277424 56-57 21.15616701115923 28.75665775388423 28.88621994573742 21.200955289219124 58-59 21.09155221438585 28.731048251952384 28.94099055430822 21.23640897935354 60-61 21.108624113652898 28.92050687016889 28.78497396110678 21.185895055071434 62-63 21.06127459822556 28.77763343397712 28.813620165142485 21.347471802654837 64-65 20.871664038142086 28.6297274924996 28.897528860079387 21.601079609278926 66-67 20.938376695830435 28.648495889000092 28.668347019624235 21.744780395545238 68-69 20.90066160804663 28.721710512447 28.664435700180114 21.713192179326256 70-71 21.10400450732712 28.43706015961583 28.756218278310993 21.702717054746063 72-73 21.07128463907483 28.570374981605866 28.6876080709774 21.6707323083419 74-75 21.03361732831035 28.893495894773118 28.377510485961892 21.695376290954634 76-77 20.967933337955017 28.657058778252864 28.569107801453193 21.805900082338933 78-79 20.829172253421017 28.840019746449812 28.480097069534782 21.85071093059439 80-81 20.950327621437204 28.765087325384485 28.22727180140171 22.0573132517766 82-83 21.163010713905816 28.52034120503791 28.453894419174432 21.86275366188184 84-85 21.178129826191938 28.617814167220327 28.248235536612466 21.95582046997527 86-87 20.9754044555171 28.621681361292296 28.38346108127553 22.019453101915072 88-89 21.15112737297366 28.538497230637766 28.26817740518821 22.04219799120036 90-91 21.114377447163363 28.49626816216306 28.36184675580002 22.02750763487356 92-93 21.12249428914767 28.577343105523717 28.176729531756685 22.123433073571924 94-95 20.93923990586234 28.42809803348253 28.29967428042145 22.332987780233683 96-97 20.994696221810127 28.50305994960083 28.143973426992297 22.358270401596748 98-99 20.890185690910286 28.506096085643218 28.020268112297597 22.5834501111489 100-101 21.197697302121167 28.565986360251355 27.859754802100294 22.376561535527184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 229843.0 1 208886.0 2 138688.0 3 70139.5 4 43299.5 5 33541.0 6 31100.0 7 31495.0 8 32330.5 9 31977.0 10 29453.0 11 25241.5 12 20139.5 13 15102.5 14 11334.0 15 8940.0 16 8160.5 17 8638.0 18 9850.0 19 11691.0 20 13872.0 21 16107.0 22 18449.0 23 21039.5 24 23913.5 25 27059.0 26 30974.0 27 35387.5 28 40459.5 29 46603.0 30 53246.5 31 60168.0 32 67708.0 33 75022.0 34 82073.0 35 88780.0 36 95516.5 37 102391.0 38 106092.5 39 107502.5 40 108918.5 41 109366.5 42 109039.5 43 109435.0 44 109808.5 45 109911.0 46 108802.0 47 105925.0 48 102537.0 49 98701.5 50 92928.5 51 85574.0 52 78580.5 53 71302.5 54 63516.5 55 54978.5 56 46036.0 57 37737.5 58 30173.5 59 23638.5 60 18144.5 61 13334.5 62 9432.0 63 6547.0 64 4411.0 65 2910.5 66 1881.0 67 1213.5 68 805.5 69 502.5 70 317.5 71 201.5 72 127.0 73 90.5 74 66.5 75 45.5 76 31.0 77 18.0 78 14.0 79 10.0 80 6.0 81 4.5 82 3.5 83 3.0 84 2.0 85 1.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.793780518634726 2 29.91972439705799 3 23.656143545711757 4 30.0515920910614 5 33.23790111215242 6 25.7332347242261 7 24.611713178881033 8 20.574833300981147 9 12.226219055985617 10-11 15.950189118949885 12-13 16.014171065621813 14-15 20.22000474379087 16-17 23.642709086890257 18-19 27.669842924522843 20-21 29.78042602041984 22-23 29.385442161962498 24-25 27.883892353100652 26-27 29.3343427574671 28-29 26.265632365899737 30-31 28.177485085462273 32-33 27.133501098044892 34-35 25.16003563496933 36-37 28.207894346462037 38-39 28.34555043454686 40-41 25.307128151545005 42-43 26.17089116230684 44-45 24.847448893191746 46-47 28.20723473876439 48-49 23.53251421723816 50-51 29.154081396666797 52-53 22.693722069315886 54-55 23.835139536643094 56-57 26.003135424998703 58-59 25.294447530092246 60-61 24.79348221441881 62-63 24.6258745523081 64-65 23.112680648375523 66-67 23.64406868643031 68-69 24.648920437581054 70-71 23.11412101620508 72-73 22.974863023711688 74-75 20.205143378521395 76-77 22.52330097845398 78-79 21.63044792208768 80-81 22.67813378944084 82-83 23.563206167251206 84-85 20.45451547237738 86-87 22.05269107825397 88-89 20.85606848504804 90-91 21.99850901737568 92-93 21.966282469861987 94-95 20.997143764055366 96-97 19.90346035582739 98-99 19.32779783374063 100-101 20.988918859907134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3714329.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.47333684215376 #Duplication Level Percentage of deduplicated Percentage of total 1 88.090285770207 66.48467810456418 2 7.800986386949252 11.775329465665537 3 2.05179203024303 4.645667730857363 4 0.7838985926309738 2.3665377012691104 5 0.39522521137920685 1.491448275346715 6 0.2248790067774091 1.0183421416344227 7 0.13589951463519753 0.7179752891323229 8 0.09686851398159783 0.5848791988105612 9 0.0744871773855375 0.5059616246315937 >10 0.325624362820168 4.273598004990179 >50 0.014775087481131856 0.7389041878214241 >100 0.004992171143416419 0.617705644567838 >500 1.4308718517646858E-4 0.0671174462641376 >1k 1.0731538569084757E-4 0.12551448074765056 >5k 0.0 0.0 >10k+ 3.577179523028161E-5 4.586340703697038 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 169876 4.573531316154277 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.692276316933691E-5 0.0 5 0.0 0.0 0.0 2.692276316933691E-5 0.0 6 0.0 0.0 0.0 5.384552633867382E-5 0.0 7 0.0 0.0 0.0 5.384552633867382E-5 0.0 8 0.0 0.0 0.0 5.384552633867382E-5 0.0 9 0.0 0.0 0.0 5.384552633867382E-5 0.0 10-11 0.0 0.0 0.0 9.422967109267918E-5 0.0 12-13 0.0 1.3461381584668455E-5 0.0 1.0769105267734764E-4 0.0 14-15 0.0 2.692276316933691E-5 0.0 1.6153657901602146E-4 0.0 16-17 0.0 2.692276316933691E-5 0.0 2.2884348693936373E-4 0.0 18-19 0.0 2.692276316933691E-5 0.0 2.423048685240322E-4 0.0 20-21 0.0 2.692276316933691E-5 0.0 2.5576625010870067E-4 0.0 22-23 0.0 2.692276316933691E-5 0.0 3.903800659553852E-4 0.0 24-25 0.0 2.692276316933691E-5 0.0 4.4422559229405904E-4 0.0 26-27 0.0 2.692276316933691E-5 0.0 5.92300789725412E-4 0.0 28-29 0.0 2.692276316933691E-5 2.692276316933691E-5 8.615284214187811E-4 0.0 30-31 0.0 2.692276316933691E-5 2.692276316933691E-5 0.0020461300008696053 0.0 32-33 0.0 2.692276316933691E-5 2.692276316933691E-5 0.0055730119760527405 0.0 34-35 0.0 2.692276316933691E-5 2.692276316933691E-5 0.010930641846750785 2.692276316933691E-5 36-37 0.0 2.692276316933691E-5 2.692276316933691E-5 0.01951900329776926 2.692276316933691E-5 38-39 0.0 2.692276316933691E-5 2.692276316933691E-5 0.033828451922271824 2.692276316933691E-5 40-41 0.0 2.692276316933691E-5 2.692276316933691E-5 0.04854174199431445 2.692276316933691E-5 42-43 0.0 2.692276316933691E-5 2.692276316933691E-5 0.06338964588220375 2.692276316933691E-5 44-45 0.0 2.692276316933691E-5 2.692276316933691E-5 0.08273365121937233 2.692276316933691E-5 46-47 0.0 2.692276316933691E-5 2.692276316933691E-5 0.10785258925636368 2.692276316933691E-5 48-49 0.0 2.692276316933691E-5 2.692276316933691E-5 0.13591956986039738 2.692276316933691E-5 50-51 0.0 2.692276316933691E-5 2.692276316933691E-5 0.1689941844139278 2.692276316933691E-5 52-53 0.0 2.692276316933691E-5 2.692276316933691E-5 0.2062418272587054 2.692276316933691E-5 54-55 0.0 2.692276316933691E-5 2.692276316933691E-5 0.24912978898745908 2.692276316933691E-5 56-57 0.0 2.692276316933691E-5 2.692276316933691E-5 0.2991118988113331 2.692276316933691E-5 58-59 1.3461381584668455E-5 2.692276316933691E-5 2.692276316933691E-5 0.3572246831123468 2.692276316933691E-5 60-61 2.692276316933691E-5 2.692276316933691E-5 2.692276316933691E-5 0.4202104875470105 2.692276316933691E-5 62-63 2.692276316933691E-5 4.0384144754005364E-5 2.692276316933691E-5 0.48614433454871664 2.692276316933691E-5 64-65 2.692276316933691E-5 5.384552633867382E-5 2.692276316933691E-5 0.554824303393695 2.692276316933691E-5 66-67 2.692276316933691E-5 5.384552633867382E-5 2.692276316933691E-5 0.6333041580323122 5.384552633867382E-5 68-69 2.692276316933691E-5 8.076828950801073E-5 2.692276316933691E-5 0.7199012257664843 5.384552633867382E-5 70-71 2.692276316933691E-5 8.076828950801073E-5 2.692276316933691E-5 0.8189769942296442 8.076828950801073E-5 72-73 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 0.9245680713797835 1.0769105267734764E-4 74-75 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 1.0293380042532583 1.0769105267734764E-4 76-77 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 1.1430193717357833 1.0769105267734764E-4 78-79 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 1.2732986227122045 1.211524342620161E-4 80-81 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 1.4180757816553138 1.3461381584668457E-4 82-83 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 1.5724374442867068 1.3461381584668457E-4 84-85 2.692276316933691E-5 1.0769105267734764E-4 2.692276316933691E-5 1.7232722249429169 1.3461381584668457E-4 86-87 2.692276316933691E-5 1.211524342620161E-4 2.692276316933691E-5 1.874928149875792 1.3461381584668457E-4 88-89 2.692276316933691E-5 1.3461381584668457E-4 2.692276316933691E-5 2.0474626776464877 1.3461381584668457E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2395 0.0 50.595024 1 GTATCAA 4765 0.0 47.843334 1 ATCAACG 5760 0.0 38.89431 3 TCAACGC 5880 0.0 38.43908 4 CAACGCA 5910 0.0 38.407616 5 TATCAAC 6110 0.0 36.21229 2 AACGCAG 6410 0.0 34.879272 6 ACGCAGA 7185 0.0 29.939419 7 CGCAGAG 7410 0.0 28.553186 8 GTACATG 5795 0.0 27.023882 1 TACATGG 6070 0.0 25.625593 2 GCAGAGT 7945 0.0 24.52996 9 ACATGGG 6185 0.0 24.474123 3 AGAGTAC 7205 0.0 20.597513 10-11 GAGTACT 5130 0.0 19.990528 12-13 GGCGTCG 315 3.8198777E-11 19.672628 8 CAGAGTA 7825 0.0 19.228508 10-11 GAGTACA 4310 0.0 18.107882 1 CATGGGG 4005 0.0 17.887848 4 AGTACTT 5960 0.0 17.81106 12-13 >>END_MODULE