FastQCFastQC Report
Sat 18 Jun 2016
SRR1931026_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931026_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714329
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76360.20558221956105666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62610.1685634202032184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.10852565833559709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.055.647391
GTATCAA81150.040.8196221
ATCAACG99500.032.8590473
CAACGCA100200.032.5741465
TCAACGC101900.032.2712364
TATCAAC107250.030.6191942
AACGCAG108950.030.3491256
ACGCAGA124250.026.191987
CGCAGAG125150.025.8140958
GTACATG97750.023.3372271
ACATGGG96450.022.4180983
TACATGG101900.022.0597172
GCAGAGT144550.021.7257449
GGCGTCG4200.020.3312388
ACGGGCG4350.019.630695
AGAGTAC138000.019.16463510-11
CAGAGTA142100.018.89544510-11
GAGTACT103500.018.65472412-13
CATGGGG59400.018.6403124
GAGTACA74200.018.1902711
Produced by FastQC (version 0.11.5)