##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931026_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3714329 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.335845316879578 31.0 31.0 34.0 30.0 34.0 2 31.492702719656766 31.0 31.0 34.0 30.0 34.0 3 31.561629031784744 31.0 31.0 34.0 30.0 34.0 4 35.33193290093581 37.0 35.0 37.0 33.0 37.0 5 35.20910990921914 37.0 35.0 37.0 33.0 37.0 6 35.23673589496246 37.0 35.0 37.0 32.0 37.0 7 35.116556449361376 37.0 35.0 37.0 32.0 37.0 8 35.118873422359734 37.0 35.0 37.0 32.0 37.0 9 36.62655004443602 39.0 35.0 39.0 32.0 39.0 10-11 36.56985097442903 38.0 35.0 39.0 32.0 39.0 12-13 36.452835895797065 38.0 35.0 39.0 32.0 39.0 14-15 37.51509990095116 39.0 36.0 41.0 32.0 41.0 16-17 37.47168317615376 39.0 36.0 41.0 32.0 41.0 18-19 37.492431472817835 39.0 36.0 41.0 32.0 41.0 20-21 37.4752896148941 39.0 36.0 41.0 32.0 41.0 22-23 37.374677229723055 39.0 36.0 41.0 32.0 41.0 24-25 37.24555646524581 39.0 36.0 40.5 31.0 41.0 26-27 36.992078515392684 39.0 36.0 40.0 31.0 41.0 28-29 36.80985206210866 39.0 36.0 40.0 30.5 41.0 30-31 36.591138534039395 38.5 35.0 40.0 30.0 41.0 32-33 36.44467439475609 38.0 35.0 40.0 30.0 41.0 34-35 36.538507089705845 38.0 35.0 40.0 30.0 41.0 36-37 36.618806250065624 39.0 35.0 40.0 30.0 41.0 38-39 36.61212994325489 39.0 35.0 40.0 30.0 41.0 40-41 36.50990811530158 39.0 35.0 40.0 30.0 41.0 42-43 36.381065328354055 39.0 35.0 40.0 30.0 41.0 44-45 36.22384675670895 38.5 35.0 40.0 30.0 41.0 46-47 36.00305856050986 38.0 35.0 40.0 29.0 41.0 48-49 35.800731303015965 38.0 35.0 40.0 28.5 41.0 50-51 35.547843634745334 38.0 34.5 40.0 27.5 41.0 52-53 35.34650107731437 38.0 34.0 40.0 27.0 41.0 54-55 35.20292023135269 38.0 34.0 40.0 27.0 41.0 56-57 35.03499636138856 38.0 34.0 40.0 26.5 41.0 58-59 34.8323617805531 38.0 33.5 40.0 26.0 41.0 60-61 34.53088364008681 37.5 33.0 40.0 26.0 41.0 62-63 34.27888711527708 37.0 33.0 40.0 26.0 41.0 64-65 33.96870928773407 37.0 32.5 40.0 25.0 41.0 66-67 33.64869617096385 36.0 32.0 39.0 24.5 41.0 68-69 33.305729379384545 36.0 32.0 39.0 23.5 40.0 70-71 32.94011690940678 35.5 31.5 39.0 23.0 40.0 72-73 32.515406551223656 35.0 31.0 38.0 22.0 40.0 74-75 31.99921304763256 35.0 31.0 37.5 21.0 39.5 76-77 30.607200923773853 33.5 29.5 36.0 20.0 38.5 78-79 31.348663917493578 34.0 30.5 36.0 21.0 39.0 80-81 31.311917845726644 34.0 31.0 36.0 21.0 38.5 82-83 31.037260700384913 34.0 30.5 36.0 20.5 37.0 84-85 30.694823344943327 34.0 30.0 35.0 20.0 37.0 86-87 30.349646732963073 34.0 30.0 35.0 18.5 36.5 88-89 29.99922314366875 34.0 30.0 35.0 18.0 36.0 90-91 29.655737146601716 34.0 29.0 35.0 13.0 36.0 92-93 29.330584474342473 34.0 29.0 35.0 7.0 35.5 94-95 28.948356217233314 34.0 29.0 35.0 2.0 35.0 96-97 28.519312505704264 34.0 29.0 35.0 2.0 35.0 98-99 28.02214464577586 34.0 29.0 35.0 2.0 35.0 100-101 26.87371393325685 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2819382584102712 1101 2 0.36423156803640566 1101 3 0.24412405325542963 1101 4 0.18058556108356782 1101 5 0.239982236903451 1101 6 0.27862174202680023 1101 7 0.3521570842320756 1101 8 0.3922488830600699 1101 9 0.5095080073112044 1101 10-11 0.40755422972610944 1101 12-13 0.3494805136148358 1101 14-15 0.4326000096299225 1101 16-17 0.5391215602158468 1101 18-19 0.4673585416357682 1101 20-21 0.4250464240064673 1101 22-23 0.4911927186279357 1101 24-25 0.43310173810711916 1101 26-27 0.458531994076381 1101 28-29 0.5824149258438425 1101 30-31 0.45043256625987027 1101 32-33 0.4663599157810907 1101 34-35 0.6056340158321447 1101 36-37 0.5539255402985717 1101 38-39 0.6275511586248115 1101 40-41 0.647682967047281 1101 42-43 0.5072210965412438 1101 44-45 0.5693266080075574 1101 46-47 0.5546004210915925 1101 48-49 0.6148291051602115 1101 50-51 0.6370850178675198 1101 52-53 0.4359825311721224 1101 54-55 0.4779639846053456 1101 56-57 0.5051491560218011 1101 58-59 0.5285657743853278 1101 60-61 0.4575109503138677 1101 62-63 0.3005671468520461 1101 64-65 0.366979214100688 1101 66-67 0.35017502819469115 1101 68-69 0.4299401520891095 1101 70-71 0.32432755162022886 1101 72-73 0.35477596717156956 1101 74-75 0.4626598614914563 1101 76-77 0.37122090808657404 1101 78-79 0.4338548677332206 1101 80-81 0.40572458065893713 1101 82-83 0.4984786742413192 1101 84-85 0.40676646227231217 1101 86-87 0.3929828259202992 1101 88-89 0.3613853364694499 1101 90-91 0.28061070959972056 1101 92-93 0.2471382780655098 1101 94-95 0.4240402234262852 1101 96-97 0.560455555114924 1101 98-99 0.5832498289048864 1101 100-101 0.8365678337288251 1102 1 0.3144362803198 1102 2 0.43089964672431336 1102 3 0.45890942854797956 1102 4 0.29850668255118507 1102 5 0.3741985878068661 1102 6 0.4035602056367438 1102 7 0.46532080355654415 1102 8 0.37367586164222644 1102 9 0.5314639794802645 1102 10-11 0.4297671645467105 1102 12-13 0.42796138551778284 1102 14-15 0.5614461663854513 1102 16-17 0.5618859397610976 1102 18-19 0.6262858946557017 1102 20-21 0.5768345724078756 1102 22-23 0.5976046818770655 1102 24-25 0.581448614237047 1102 26-27 0.6027872108645056 1102 28-29 0.625370957015214 1102 30-31 0.6194723111278009 1102 32-33 0.6670371564696893 1102 34-35 0.7135397210535501 1102 36-37 0.720672364385436 1102 38-39 0.657306783134068 1102 40-41 0.6311488538747838 1102 42-43 0.7315235454399414 1102 44-45 0.7486794282426246 1102 46-47 0.7455007510506988 1102 48-49 0.7990530204631341 1102 50-51 0.6510563124842434 1102 52-53 0.6571453004080112 1102 54-55 0.7806127347396057 1102 56-57 0.6988021709645125 1102 58-59 0.6989975200520604 1102 60-61 0.5041062140920403 1102 62-63 0.5069824384369568 1102 64-65 0.5217459960992343 1102 66-67 0.5103997513754877 1102 68-69 0.6934756616497495 1102 70-71 0.6338963657190533 1102 72-73 0.3752589994670146 1102 74-75 0.5238019950454635 1102 76-77 0.37996497570625465 1102 78-79 0.5053749035582165 1102 80-81 0.5583585802141471 1102 82-83 0.4387497218047507 1102 84-85 0.5916506740089353 1102 86-87 0.5692599613124578 1102 88-89 0.4813423404226107 1102 90-91 0.5518715890973276 1102 92-93 0.5111046558225425 1102 94-95 0.6498932229376244 1102 96-97 0.612635539411233 1102 98-99 0.7711271978857042 1102 100-101 1.0301605604074062 1103 1 0.3551477257526088 1103 2 0.4462272244503751 1103 3 0.37804964391731843 1103 4 0.27418370758088173 1103 5 0.3153946083458692 1103 6 0.34669429068274127 1103 7 0.39625764482988757 1103 8 0.40682068684485273 1103 9 0.46903128976993713 1103 10-11 0.4608298679520644 1103 12-13 0.4410567387633648 1103 14-15 0.4995622066707739 1103 16-17 0.5348701108581082 1103 18-19 0.5299948022030634 1103 20-21 0.5340020787452175 1103 22-23 0.5207500437926242 1103 24-25 0.5986200368094217 1103 26-27 0.5852592575573539 1103 28-29 0.40544236342722684 1103 30-31 0.45038694664074086 1103 32-33 0.646631374950779 1103 34-35 0.5194079249997472 1103 36-37 0.4426836695651275 1103 38-39 0.41365931152382274 1103 40-41 0.46972823666670394 1103 42-43 0.5775669979550031 1103 44-45 0.5019164412641075 1103 46-47 0.6045528570296881 1103 48-49 0.4013490312115522 1103 50-51 0.29786396164620044 1103 52-53 0.40898685980090477 1103 54-55 0.4745278550635703 1103 56-57 0.4593815348830077 1103 58-59 0.4961911012012905 1103 60-61 0.2865751348743899 1103 62-63 0.24853879388482625 1103 64-65 0.18164973574982213 1103 66-67 0.2898755391344281 1103 68-69 0.17190466750404454 1103 70-71 0.2260982659187576 1103 72-73 0.23341461790776208 1103 74-75 0.326657381579043 1103 76-77 0.309141206872642 1103 78-79 0.381028395790036 1103 80-81 0.26488357924098693 1103 82-83 0.3119735558696277 1103 84-85 0.2462244413680601 1103 86-87 0.27007788823220835 1103 88-89 0.2717450284697165 1103 90-91 0.44890896319095575 1103 92-93 0.31753316448013535 1103 94-95 0.37843945043549 1103 96-97 0.44820586473004553 1103 98-99 0.5395634346197546 1103 100-101 0.7547697626160677 1104 1 0.25682756906623183 1104 2 0.34951747782155707 1104 3 0.35132737235473854 1104 4 0.09748975974125074 1104 5 0.2922965836102023 1104 6 0.27571885377211913 1104 7 0.25201449885549465 1104 8 0.3437902978982166 1104 9 0.3518444661493234 1104 10-11 0.32580927591745024 1104 12-13 0.2826900744092242 1104 14-15 0.45491697420123955 1104 16-17 0.4528690627656857 1104 18-19 0.4271471858392957 1104 20-21 0.390843361546132 1104 22-23 0.5063718842497877 1104 24-25 0.3902506776881225 1104 26-27 0.47123455686284643 1104 28-29 0.5690160919701484 1104 30-31 0.46424786856071876 1104 32-33 0.5104914870072221 1104 34-35 0.4585196967747507 1104 36-37 0.4544325677207013 1104 38-39 0.5222057512490466 1104 40-41 0.3538712018050134 1104 42-43 0.4443885229524085 1104 44-45 0.5198434156540657 1104 46-47 0.568854215708356 1104 48-49 0.468952425700806 1104 50-51 0.42838278746161507 1104 52-53 0.4318474081802819 1104 54-55 0.5057664417212635 1104 56-57 0.5436295959407005 1104 58-59 0.5475540800313183 1104 60-61 0.4813858016801973 1104 62-63 0.30245943643199524 1104 64-65 0.34798932744099176 1104 66-67 0.33418414071653757 1104 68-69 0.19925017564252556 1104 70-71 0.23889758574805597 1104 72-73 0.2008552369932488 1104 74-75 0.21943270364672074 1104 76-77 0.27926731194218846 1104 78-79 0.3686749667561564 1104 80-81 0.33634411007571785 1104 82-83 0.30224924488432237 1104 84-85 0.41925966942849513 1104 86-87 0.4447109842158028 1104 88-89 0.5728286402145422 1104 90-91 0.49501363425449085 1104 92-93 0.510581159716704 1104 94-95 0.48479847025120293 1104 96-97 0.4883008466722387 1104 98-99 0.6304899672399067 1104 100-101 0.8861152225927533 1105 1 0.2857504203941801 1105 2 0.3982922837354259 1105 3 0.35688890349554825 1105 4 0.15319029443227095 1105 5 0.27642136827729047 1105 6 0.3317515300596412 1105 7 0.39082982159892055 1105 8 0.3752267151475195 1105 9 0.4054011525513985 1105 10-11 0.4572708434118624 1105 12-13 0.4528101940913274 1105 14-15 0.5569053507734054 1105 16-17 0.5555899289398525 1105 18-19 0.554004160889221 1105 20-21 0.4628126875542833 1105 22-23 0.4838077872422204 1105 24-25 0.5964586555598004 1105 26-27 0.5190504098381652 1105 28-29 0.5841591649842002 1105 30-31 0.5239492092264513 1105 32-33 0.583951248073646 1105 34-35 0.6702797249574743 1105 36-37 0.696082769693632 1105 38-39 0.7913226463377327 1105 40-41 0.7319916276519436 1105 42-43 0.7550165539528422 1105 44-45 0.7235827660428953 1105 46-47 0.7546684772934071 1105 48-49 0.6709270568062351 1105 50-51 0.7031425126745177 1105 52-53 0.6855920233815382 1105 54-55 0.6998451044089009 1105 56-57 0.7324030919025617 1105 58-59 0.8067309675881233 1105 60-61 0.9066714455368867 1105 62-63 0.6953527661924781 1105 64-65 0.7549469645931808 1105 66-67 0.8368589154721633 1105 68-69 0.6252376787281406 1105 70-71 0.612033803948556 1105 72-73 0.7107810753729566 1105 74-75 0.7454524415535211 1105 76-77 0.685265127947968 1105 78-79 0.795999891947897 1105 80-81 0.799892403338827 1105 82-83 0.710418587645421 1105 84-85 0.8396235471475997 1105 86-87 0.8125864184760054 1105 88-89 0.8810785109904877 1105 90-91 0.8956720761450612 1105 92-93 0.8233806480158314 1105 94-95 0.9643112306145873 1105 96-97 0.7898140148849428 1105 98-99 0.8380271713355718 1105 100-101 0.9763081619930993 1106 1 0.27020434614573574 1106 2 0.23907288021706918 1106 3 0.3222803361519482 1106 4 0.13606681666438902 1106 5 0.11574015845675234 1106 6 0.1572250609914576 1106 7 0.2758634040725454 1106 8 0.20659597361697024 1106 9 0.3543168829883925 1106 10-11 0.2811935797408083 1106 12-13 0.31135449908259716 1106 14-15 0.3511301111045384 1106 16-17 0.28391531597496567 1106 18-19 0.2933448310081772 1106 20-21 0.2549422820047553 1106 22-23 0.32418352400606665 1106 24-25 0.341059807436217 1106 26-27 0.4520158799523841 1106 28-29 0.421392220291402 1106 30-31 0.4297632103398499 1106 32-33 0.49284002738320254 1106 34-35 0.45808237275350194 1106 36-37 0.4172740030593616 1106 38-39 0.43228476278535055 1106 40-41 0.34792196160125144 1106 42-43 0.38999651440727945 1106 44-45 0.3677623383709161 1106 46-47 0.3000605943715584 1106 48-49 0.3389589966676638 1106 50-51 0.2764864545450223 1106 52-53 0.2842558650569984 1106 54-55 0.41878690754226255 1106 56-57 0.48006157460959287 1106 58-59 0.4686858350932255 1106 60-61 0.463363998974323 1106 62-63 0.2891773553642807 1106 64-65 0.1740186057664843 1106 66-67 0.2531575033538047 1106 68-69 0.35910897025354416 1106 70-71 0.39381199094228236 1106 72-73 0.3741375928195083 1106 74-75 0.568811742494848 1106 76-77 0.52809077123705 1106 78-79 0.4736351582068181 1106 80-81 0.47782077186598926 1106 82-83 0.5533620511751707 1106 84-85 0.5074784535084653 1106 86-87 0.44229936283144156 1106 88-89 0.5377866390646595 1106 90-91 0.5103892455922576 1106 92-93 0.49729026177022817 1106 94-95 0.6678718674178299 1106 96-97 0.6606435556056098 1106 98-99 0.672614963514949 1106 100-101 0.9019327372608963 1107 1 0.10603121650809655 1107 2 0.09845685732370058 1107 3 0.1436634062637978 1107 4 0.11506354734378732 1107 5 0.1606404610765324 1107 6 0.2027035662368064 1107 7 0.175191829713512 1107 8 0.15831631636773125 1107 9 0.16004852937779646 1107 10-11 0.1491929266102474 1107 12-13 0.1886480863779667 1107 14-15 0.21092229481106983 1107 16-17 0.18390794107513386 1107 18-19 0.2706867284821328 1107 20-21 0.21825461150113767 1107 22-23 0.18410201760876532 1107 24-25 0.12848434570592104 1107 26-27 0.22173227510134552 1107 28-29 0.20536954961355747 1107 30-31 0.27284053727792923 1107 32-33 0.17539362116009016 1107 34-35 0.18747545517606312 1107 36-37 0.24261174858260404 1107 38-39 0.34116286499177306 1107 40-41 0.31237543567684156 1107 42-43 0.3667938089324849 1107 44-45 0.3407030384126486 1107 46-47 0.27086326117725434 1107 48-49 0.26813752583231576 1107 50-51 0.36055176524313026 1107 52-53 0.3672843997626458 1107 54-55 0.4111343027879002 1107 56-57 0.3912591024593439 1107 58-59 0.37802352586145105 1107 60-61 0.4525911107827838 1107 62-63 0.3317056714427906 1107 64-65 0.3201236565642134 1107 66-67 0.23487593884806302 1107 68-69 0.2910467336966249 1107 70-71 0.3452400230544015 1107 72-73 0.23087348311663192 1107 74-75 0.3775459752079122 1107 76-77 0.4091402522301131 1107 78-79 0.48884647856015206 1107 80-81 0.39184365265642995 1107 82-83 0.4885599657159325 1107 84-85 0.42739519573619944 1107 86-87 0.39709767831524445 1107 88-89 0.4791998388516099 1107 90-91 0.398748129962474 1107 92-93 0.18823765083300614 1107 94-95 0.4022491966351929 1107 96-97 0.4084570222512838 1107 98-99 0.5719135227765264 1107 100-101 0.8612784054962468 1108 1 0.07820053205760402 1108 2 0.1647150404326574 1108 3 0.08053004155207333 1108 4 0.03782626216743523 1108 5 0.11390140858853925 1108 6 0.08692877842901936 1108 7 0.1404749606972686 1108 8 0.1357943659248093 1108 9 0.2094158108993227 1108 10-11 0.09139757684700811 1108 12-13 0.18548712381818433 1108 14-15 0.19377735780966532 1108 16-17 0.15814908363861946 1108 18-19 0.19215944627813997 1108 20-21 0.1094986865203822 1108 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-1.318501448039381 2316 82-83 -1.0918833360990021 2316 84-85 -1.017338617233662 2316 86-87 -0.7728931622215498 2316 88-89 -1.0272711867296564 2316 90-91 -0.9521363629977024 2316 92-93 -1.0350716576833285 2316 94-95 -1.3046745192314653 2316 96-97 -1.114098166222096 2316 98-99 -1.1917569905229648 2316 100-101 -1.67552992130652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 44.0 5 99.0 6 141.0 7 485.0 8 1116.0 9 2151.0 10 3889.0 11 6080.0 12 8724.0 13 11502.0 14 13929.0 15 17835.0 16 21331.0 17 25104.0 18 21743.0 19 17375.0 20 17360.0 21 18222.0 22 20680.0 23 24058.0 24 30025.0 25 38191.0 26 49616.0 27 63820.0 28 82286.0 29 103153.0 30 126137.0 31 152864.0 32 185450.0 33 224130.0 34 278381.0 35 346608.0 36 455989.0 37 628338.0 38 605427.0 39 112001.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.773656756723284 22.113805947042984 14.788639867405529 23.323897428828204 2 14.836903111158978 22.188931220635155 40.844280502416495 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28.262547555695793 30.28408361241021 20.34157448088201 22-23 20.81618460880716 28.078431066367752 30.13127036207714 20.97411396274795 24-25 20.67655858251022 28.284642178061215 30.28394891382995 20.754850325598614 26-27 20.75286027943843 28.455778915442885 30.092751266411522 20.698609538707164 28-29 20.706691272313787 28.50952535502091 30.08934197573259 20.69444139693271 30-31 21.028963450547273 28.288895425580108 30.031871978977282 20.650269144895333 32-33 20.89481119322521 28.48624974821814 29.855593004180385 20.763346054376264 34-35 20.921799239757092 28.437740403192084 30.09264332910677 20.547817027944056 36-37 20.931764978279862 28.373540604610024 30.069003673644662 20.625690743465448 38-39 20.838485132194542 28.48902927657638 30.13009792512353 20.54238766610555 40-41 20.970872636286696 28.37916552074419 30.052811376920253 20.59715046604886 42-43 20.86492820649453 28.42297968391821 29.82788487674819 20.884207232839074 44-45 20.827175945726946 28.69375607577151 29.72418538384606 20.754882594655488 46-47 20.94117380968953 28.4889088079683 29.50974517325448 21.060172209087686 48-49 21.006479532236174 28.607385904752263 29.45228707468688 20.933847488324687 50-51 21.094196425982492 28.754760921849066 29.11668606257788 21.034356589590566 52-53 20.997875132391503 28.865437674615613 29.099258014613298 21.037429178379583 54-55 21.037520199113917 28.79935201269478 29.16298318663591 21.000144601555395 56-57 21.062532023399598 28.80744927210796 29.096995999817988 21.033022704674455 58-59 20.959709825110117 28.77002172407131 29.311528029572205 20.95874042124637 60-61 21.032510629947364 28.95657234537045 29.178160990802205 20.832756033879978 62-63 21.02182047613316 28.8130781449276 29.146748625459505 21.018352753479732 64-65 20.771253806949055 28.71623673049702 29.197602414857254 21.314907047696668 66-67 20.870662789221047 28.72289502624347 29.10926307615634 21.29717910837914 68-69 20.85509381053558 28.78540540577012 29.09112377646866 21.26837700722564 70-71 20.963506265477154 28.44348926987029 29.215510258824096 21.377494205828455 72-73 21.029052128369006 28.651241518249233 29.065570813096635 21.25413554028513 74-75 20.913853809629604 28.86427424333676 28.927253590268194 21.294618356765447 76-77 20.767545451136368 28.76737440826315 29.04973004092889 21.415350099671592 78-79 20.745688939123756 28.78689074553278 29.05519197201805 21.412228343325413 80-81 20.87029997481375 28.80956813593628 28.89154796082253 21.42858392842744 82-83 20.96681527134511 28.725619082208386 29.044788439580877 21.26277720686563 84-85 21.028724165252996 28.66166405829963 28.8631944020037 21.446417374443673 86-87 20.726394996242927 28.787864510655897 28.982650702186046 21.503089790915126 88-89 20.876893832569646 28.657698777961492 28.88476551536328 21.580641874105574 90-91 20.865586496454327 28.546546598293105 29.121314792846515 21.466552112406053 92-93 20.92572591236031 28.625468778989436 28.930075825935596 21.518729482714654 94-95 20.59749022443778 28.60585210446346 29.001155419155456 21.7955022519433 96-97 20.646886953291006 28.549030746481023 28.98776427226732 21.816318027960648 98-99 20.58631585947287 28.601585912287252 28.94556459592029 21.866533632319594 100-101 20.605067564020313 28.56584594417996 28.82375255396062 22.005333937839108 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 147.0 2 147.0 3 181.5 4 160.5 5 179.5 6 307.5 7 673.0 8 1174.5 9 1458.5 10 1410.0 11 1396.5 12 1608.0 13 1899.0 14 2289.0 15 2627.0 16 2945.0 17 3375.0 18 3961.5 19 4563.0 20 5382.5 21 6497.0 22 7845.5 23 9952.0 24 12945.0 25 16820.0 26 22597.0 27 29651.0 28 37433.5 29 47675.5 30 58591.0 31 68996.5 32 81960.5 33 95042.5 34 106224.0 35 116258.0 36 128932.0 37 142365.5 38 148157.5 39 149438.0 40 150816.0 41 150478.0 42 149051.5 43 151751.5 44 155958.5 45 158880.0 46 157831.5 47 153469.0 48 149354.5 49 143430.0 50 134266.0 51 123321.5 52 112966.0 53 102006.5 54 90222.0 55 76780.5 56 62398.5 57 48822.0 58 37058.0 59 27813.0 60 19707.0 61 13070.5 62 8656.5 63 5524.5 64 3264.5 65 1824.5 66 1011.0 67 547.0 68 279.5 69 147.0 70 95.5 71 63.0 72 34.0 73 20.0 74 13.0 75 9.5 76 7.0 77 6.5 78 5.0 79 2.0 80 1.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003957646185892526 2 0.007511450924244998 3 0.0023153576325629743 4 0.1693711030982985 5 0.0018845934218535837 6 2.96150394862706E-4 7 5.384552633867382E-5 8 7.269146055720966E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6922763169336914E-4 24-25 4.44225592294059E-4 26-27 0.00253073973791767 28-29 1.48075197431353E-4 30-31 0.006771074937088234 32-33 0.02145744224596152 34-35 0.013892145795377847 36-37 9.15373947757455E-4 38-39 3.0961177644737444E-4 40-41 0.0019519003297769262 42-43 0.012074859281447605 44-45 0.007928753753369721 46-47 0.011132562570520813 48-49 0.010863334938827443 50-51 0.18664205567142814 52-53 0.11349290814033974 54-55 0.018199787902471752 56-57 0.0066768452659955535 58-59 0.01902093217913653 60-61 0.1218793488675882 62-63 0.23495495417880322 64-65 0.24052796615485597 66-67 0.25071823201445 68-69 0.24557598424910665 70-71 0.233299204243889 72-73 0.15954429454149052 74-75 0.26761226590320886 76-77 0.20612067482444338 78-79 0.005923007897254121 80-81 1.3461381584668455E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 6.730690792334228E-5 90-91 0.0060037761867621315 92-93 0.018630552113181143 94-95 0.002490355593163664 96-97 4.0384144754005364E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3714329.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.995505746623586 #Duplication Level Percentage of deduplicated Percentage of total 1 70.07442280479921 36.43555053638268 2 16.428027224191872 17.083671678823144 3 5.874581714944604 9.16355541955236 4 2.7435667147149787 5.706125555248314 5 1.4663063887421346 3.8120671131076262 6 0.8489686486417849 2.6485532549494306 7 0.5487824810971085 1.9973955854671734 8 0.3658221501221058 1.5216886167132915 9 0.28542908638008446 1.335692673101628 >10 1.226944492007289 11.132028271113798 >50 0.08660595609253335 3.137935421670942 >100 0.04795333105411822 4.482274211050984 >500 0.002019425641645939 0.7107225368233985 >1k 4.6602128487169487E-4 0.4585853712601906 >5k 1.0356028552704328E-4 0.37415375473509466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7636 0.20558221956105666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6261 0.1685634202032184 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4031 0.10852565833559709 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.692276316933691E-5 0.0 6 0.0 0.0 0.0 2.692276316933691E-5 0.0 7 0.0 0.0 0.0 2.692276316933691E-5 0.0 8 0.0 0.0 0.0 2.692276316933691E-5 0.0 9 0.0 0.0 0.0 2.692276316933691E-5 0.0 10-11 0.0 0.0 0.0 1.0769105267734764E-4 0.0 12-13 0.0 0.0 0.0 1.6153657901602146E-4 1.3461381584668455E-5 14-15 0.0 0.0 0.0 1.749979606006899E-4 2.692276316933691E-5 16-17 0.0 0.0 0.0 2.1538210535469528E-4 2.692276316933691E-5 18-19 0.0 0.0 0.0 2.1538210535469528E-4 2.692276316933691E-5 20-21 0.0 0.0 0.0 2.1538210535469528E-4 2.692276316933691E-5 22-23 0.0 0.0 0.0 2.8268901327803755E-4 2.692276316933691E-5 24-25 0.0 0.0 0.0 3.365345396167114E-4 2.692276316933691E-5 26-27 0.0 0.0 0.0 6.326849344794174E-4 2.692276316933691E-5 28-29 0.0 0.0 0.0 0.001238447105789498 4.0384144754005364E-5 30-31 0.0 0.0 0.0 0.0037018799357838254 5.384552633867382E-5 32-33 0.0 0.0 0.0 0.011078717044182139 5.384552633867382E-5 34-35 0.0 0.0 0.0 0.02196897474617892 5.384552633867382E-5 36-37 0.0 0.0 0.0 0.04007453297755799 5.384552633867382E-5 38-39 0.0 0.0 0.0 0.06989149318759862 5.384552633867382E-5 40-41 0.0 0.0 0.0 0.10503916050516796 5.384552633867382E-5 42-43 0.0 0.0 0.0 0.14042913269126134 5.384552633867382E-5 44-45 0.0 0.0 0.0 0.18417862284143383 5.384552633867382E-5 46-47 0.0 0.0 0.0 0.23989528122037657 5.384552633867382E-5 48-49 0.0 0.0 0.0 0.30180417512826674 5.384552633867382E-5 50-51 0.0 0.0 0.0 0.3697033838413345 5.384552633867382E-5 52-53 0.0 0.0 0.0 0.4404294826871825 5.384552633867382E-5 54-55 0.0 0.0 0.0 0.5190843352864003 5.384552633867382E-5 56-57 0.0 0.0 0.0 0.6102313499961904 5.384552633867382E-5 58-59 0.0 0.0 0.0 0.7159705023437611 5.384552633867382E-5 60-61 0.0 0.0 0.0 0.8330441379856227 5.384552633867382E-5 62-63 0.0 0.0 0.0 0.956175395340585 5.384552633867382E-5 64-65 0.0 0.0 0.0 1.0829277643418234 5.384552633867382E-5 66-67 0.0 0.0 0.0 1.2304914292729587 5.384552633867382E-5 68-69 0.0 0.0 0.0 1.3959991158564575 5.384552633867382E-5 70-71 0.0 0.0 0.0 1.5845796104760779 5.384552633867382E-5 72-73 0.0 0.0 0.0 1.7829465295077522 5.384552633867382E-5 74-75 0.0 0.0 0.0 1.9846922553171784 5.384552633867382E-5 76-77 0.0 0.0 0.0 2.2045570007395683 5.384552633867382E-5 78-79 0.0 0.0 0.0 2.4594751838084346 5.384552633867382E-5 80-81 0.0 0.0 0.0 2.7444660933374507 5.384552633867382E-5 82-83 0.0 0.0 0.0 3.0501067622173483 5.384552633867382E-5 84-85 0.0 0.0 0.0 3.3535936100436983 5.384552633867382E-5 86-87 0.0 0.0 0.0 3.6665976546504093 5.384552633867382E-5 88-89 0.0 0.0 0.0 4.022153126446257 5.384552633867382E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4330 0.0 55.64739 1 GTATCAA 8115 0.0 40.819622 1 ATCAACG 9950 0.0 32.859047 3 CAACGCA 10020 0.0 32.574146 5 TCAACGC 10190 0.0 32.271236 4 TATCAAC 10725 0.0 30.619194 2 AACGCAG 10895 0.0 30.349125 6 ACGCAGA 12425 0.0 26.19198 7 CGCAGAG 12515 0.0 25.814095 8 GTACATG 9775 0.0 23.337227 1 ACATGGG 9645 0.0 22.418098 3 TACATGG 10190 0.0 22.059717 2 GCAGAGT 14455 0.0 21.725744 9 GGCGTCG 420 0.0 20.331238 8 ACGGGCG 435 0.0 19.63069 5 AGAGTAC 13800 0.0 19.164635 10-11 CAGAGTA 14210 0.0 18.895445 10-11 GAGTACT 10350 0.0 18.654724 12-13 CATGGGG 5940 0.0 18.640312 4 GAGTACA 7420 0.0 18.190271 1 >>END_MODULE