FastQCFastQC Report
Sat 18 Jun 2016
SRR1931025_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931025_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531010
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN659334.306503549944154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11800.053.7302251
GTATCAA23650.047.130751
ATCAACG29150.038.475813
TCAACGC29600.037.7191284
CAACGCA31000.036.0104455
TATCAAC31000.035.949112
AACGCAG33550.034.0509576
ACGCAGA37550.028.9798157
CGCAGAG38300.028.4387958
GCAGAGT40900.025.0942139
GTACATG23700.022.8684371
AGAGTAC35900.022.35529110-11
TACATGG24150.021.8028562
GAGTACT26800.021.4119912-13
CGTAGAC1200.003420816720.9560893
CAGAGTA39650.020.64580310-11
ACATGGG24200.020.5750683
ACGCGAC600.004813052719.75725654-55
GAGTACA18200.019.3845921
TAACGCA2101.1954851E-519.1589224
Produced by FastQC (version 0.11.5)