##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931025_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1531010 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.96338626135688 28.0 2.0 31.0 2.0 33.0 2 19.74220024689584 27.0 2.0 31.0 2.0 33.0 3 20.35697154166204 27.0 2.0 31.0 2.0 33.0 4 21.91235850843561 30.0 2.0 35.0 2.0 37.0 5 21.274592589205817 30.0 2.0 35.0 2.0 37.0 6 22.18977407071149 30.0 2.0 35.0 2.0 37.0 7 22.36166648160365 30.0 2.0 35.0 2.0 37.0 8 22.76114721654333 30.0 2.0 35.0 2.0 37.0 9 24.021285948491517 32.0 2.0 37.0 2.0 39.0 10-11 24.328187601648587 32.0 2.0 37.0 2.0 39.0 12-13 24.208719407449983 32.0 2.0 37.0 2.0 39.0 14-15 24.16804364439161 32.0 2.0 38.0 2.0 40.0 16-17 23.036830588957613 30.5 2.0 38.0 2.0 40.0 18-19 22.0173983187569 28.5 2.0 37.0 2.0 40.0 20-21 21.685532099725016 27.0 2.0 37.0 2.0 40.0 22-23 21.310690655188406 27.0 2.0 37.0 2.0 39.0 24-25 21.133468755919296 27.0 2.0 37.0 2.0 39.0 26-27 20.85112180847937 26.5 2.0 37.0 2.0 39.0 28-29 20.637455013357197 25.0 2.0 36.0 2.0 39.0 30-31 20.597622157921897 25.0 2.0 36.0 2.0 39.0 32-33 20.468055401336372 25.0 2.0 36.0 2.0 39.0 34-35 20.125319233708467 24.5 2.0 36.0 2.0 39.0 36-37 19.649643046093757 24.0 2.0 35.5 2.0 39.0 38-39 19.510212539434754 24.0 2.0 35.0 2.0 39.0 40-41 19.355561361454203 23.5 2.0 35.0 2.0 38.5 42-43 19.189644417737313 23.0 2.0 35.0 2.0 38.0 44-45 18.78586455999634 20.5 2.0 35.0 2.0 38.0 46-47 18.429888113075684 18.0 2.0 35.0 2.0 38.0 48-49 18.163375484157516 16.5 2.0 34.0 2.0 38.0 50-51 15.972891424615122 10.0 2.0 32.0 2.0 37.5 52-53 17.830393988282246 17.0 2.0 33.5 2.0 38.0 54-55 18.56188529140894 20.0 2.0 35.0 2.0 38.5 56-57 18.511883985081745 19.0 2.0 35.0 2.0 39.0 58-59 18.22326634052031 17.5 2.0 35.0 2.0 39.0 60-61 17.917531890712667 13.5 2.0 34.5 2.0 38.5 62-63 17.472377058281786 10.5 2.0 34.0 2.0 38.0 64-65 17.101351395484027 8.0 2.0 34.0 2.0 38.0 66-67 16.725447253773652 7.5 2.0 33.5 2.0 38.0 68-69 16.27300344217216 4.5 2.0 33.0 2.0 37.5 70-71 15.90076322166413 2.0 2.0 32.0 2.0 37.0 72-73 15.48791092154852 2.0 2.0 32.0 2.0 36.0 74-75 15.031234283250926 2.0 2.0 31.0 2.0 36.0 76-77 14.600895487292703 2.0 2.0 31.0 2.0 35.0 78-79 14.206691334478547 2.0 2.0 30.5 2.0 35.0 80-81 13.759388573556018 2.0 2.0 30.0 2.0 34.0 82-83 13.290228345993821 2.0 2.0 29.5 2.0 34.0 84-85 12.873097497730257 2.0 2.0 29.0 2.0 34.0 86-87 12.381864586122887 2.0 2.0 29.0 2.0 34.0 88-89 11.949207059392165 2.0 2.0 28.5 2.0 34.0 90-91 11.468994977171931 2.0 2.0 26.5 2.0 34.0 92-93 10.927806480689217 2.0 2.0 26.0 2.0 33.0 94-95 10.35598559121103 2.0 2.0 25.0 2.0 33.0 96-97 9.72949000986277 2.0 2.0 22.0 2.0 32.0 98-99 9.073268300011105 2.0 2.0 10.0 2.0 32.0 100-101 8.193793639492885 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.971742403561695 1101 2 9.53166662189868 1101 3 8.874845625246707 1101 4 11.002437273829365 1101 5 11.601399873926418 1101 6 10.68216969939531 1101 7 10.414449419933796 1101 8 9.84732398727893 1101 9 9.946044611728855 1101 10-11 9.31609180473976 1101 12-13 9.320194619617201 1101 14-15 9.957564142037512 1101 16-17 10.87216411758439 1101 18-19 11.930519613423545 1101 20-21 11.832174780508833 1101 22-23 11.995841952198663 1101 24-25 11.780403605440235 1101 26-27 12.064423994868772 1101 28-29 11.946806824996802 1101 30-31 11.496766788774625 1101 32-33 11.305156250496825 1101 34-35 11.029683297728521 1101 36-37 11.52253293650356 1101 38-39 11.265055528251171 1101 40-41 10.775644434780958 1101 42-43 10.484373095199587 1101 44-45 10.457142364222442 1101 46-47 10.03745566778122 1101 48-49 10.316783510747452 1101 50-51 11.490233502813311 1101 52-53 10.936557203393466 1101 54-55 10.643133697923783 1101 56-57 10.671512402969949 1101 58-59 10.530903025766747 1101 60-61 10.333263998200888 1101 62-63 10.331448233400206 1101 64-65 10.07976749051224 1101 66-67 9.69177845875392 1101 68-69 9.591014311413694 1101 70-71 9.496828659218036 1101 72-73 9.49750235052543 1101 74-75 9.210274300328335 1101 76-77 9.069030627276552 1101 78-79 8.394848417785182 1101 80-81 8.473675080403877 1101 82-83 8.31864784933099 1101 84-85 7.865572379780938 1101 86-87 7.5409183066644445 1101 88-89 7.52843742340105 1101 90-91 7.1776001826568585 1101 92-93 6.844280028016554 1101 94-95 6.330594897427991 1101 96-97 6.220874651105188 1101 98-99 5.821690013876106 1101 100-101 4.799088860848801 1102 1 7.869414581534439 1102 2 8.949357945386634 1102 3 8.39223381141051 1102 4 10.226919214156077 1102 5 11.154673509188864 1102 6 10.119020479001929 1102 7 9.720627411265646 1102 8 9.338258523867623 1102 9 9.129211893344515 1102 10-11 8.44111116711355 1102 12-13 8.654138642220058 1102 14-15 8.989759122690629 1102 16-17 9.914237006853298 1102 18-19 10.854328285288627 1102 20-21 11.087044030271056 1102 22-23 11.146820061873342 1102 24-25 11.051588365857967 1102 26-27 11.0572041529277 1102 28-29 11.077805288398825 1102 30-31 11.080497438018252 1102 32-33 10.972375906559108 1102 34-35 10.663600058109079 1102 36-37 10.887780496069968 1102 38-39 10.664607792719512 1102 40-41 10.603276323526309 1102 42-43 10.010813965607994 1102 44-45 10.105701338915047 1102 46-47 9.713679602153615 1102 48-49 9.398450905439375 1102 50-51 11.588774431773537 1102 52-53 10.748031965700964 1102 54-55 10.45636385303963 1102 56-57 10.205966972645061 1102 58-59 10.444229682953594 1102 60-61 10.312727877305324 1102 62-63 10.276854084244867 1102 64-65 9.829833322583145 1102 66-67 9.693928456740231 1102 68-69 9.24278898914634 1102 70-71 8.949081045436984 1102 72-73 8.718409996649395 1102 74-75 8.158552752949381 1102 76-77 8.080002122764347 1102 78-79 7.897141310090111 1102 80-81 7.716417288210172 1102 82-83 7.897574693635818 1102 84-85 7.448581586976923 1102 86-87 7.001575124853304 1102 88-89 6.826405768243378 1102 90-91 6.446259702284108 1102 92-93 6.528792607363561 1102 94-95 5.968879034841981 1102 96-97 5.598364652375359 1102 98-99 5.096006444631609 1102 100-101 4.561678276290992 1103 1 7.847880920585965 1103 2 9.288769745561495 1103 3 8.612814021847967 1103 4 10.639280907303295 1103 5 11.217493461554024 1103 6 10.42341916452154 1103 7 10.046029895512984 1103 8 9.595899694840519 1103 9 9.546489321063166 1103 10-11 8.93808122952122 1103 12-13 8.563574410557965 1103 14-15 9.456151311179887 1103 16-17 10.213717696654015 1103 18-19 11.037193309859763 1103 20-21 11.438428983088453 1103 22-23 11.68307680762992 1103 24-25 11.401473047337664 1103 26-27 11.271519477477728 1103 28-29 11.12693550034371 1103 30-31 11.045213362526074 1103 32-33 11.190596986483133 1103 34-35 10.909472710284302 1103 36-37 11.236826291843126 1103 38-39 10.963501337897238 1103 40-41 10.466325405500442 1103 42-43 9.902399105346905 1103 44-45 10.192652633798822 1103 46-47 10.075724357798943 1103 48-49 10.32030527246883 1103 50-51 11.638025238249462 1103 52-53 10.829808624629482 1103 54-55 10.508446369847494 1103 56-57 10.041631297767918 1103 58-59 9.99372318592324 1103 60-61 9.95554760355574 1103 62-63 9.861834412261995 1103 64-65 9.37989738842776 1103 66-67 9.291752479170814 1103 68-69 9.226772151134945 1103 70-71 8.972431538367129 1103 72-73 8.637964022999299 1103 74-75 8.353645686109862 1103 76-77 8.05256950495856 1103 78-79 7.473153173577101 1103 80-81 7.290146900197266 1103 82-83 7.0341400860202725 1103 84-85 6.567168595931067 1103 86-87 6.446519199139534 1103 88-89 6.183757889199692 1103 90-91 5.967772259189653 1103 92-93 5.54859875165492 1103 94-95 5.511075061252184 1103 96-97 5.12212931214804 1103 98-99 4.732170483342452 1103 100-101 4.126064073270099 1104 1 8.072424436512925 1104 2 9.398202268848475 1104 3 8.677043515235795 1104 4 10.668463678639206 1104 5 11.412296651389205 1104 6 10.459148576460464 1104 7 10.251927906678887 1104 8 9.608182189250162 1104 9 9.624043114360664 1104 10-11 8.90931495825195 1104 12-13 8.807606797603146 1104 14-15 9.180549885083657 1104 16-17 10.336417875841597 1104 18-19 11.014193620846473 1104 20-21 11.303453158603428 1104 22-23 11.311509878845879 1104 24-25 11.183108911510516 1104 26-27 11.323593092476177 1104 28-29 11.302749619140638 1104 30-31 10.86700705245751 1104 32-33 10.752764341763243 1104 34-35 10.736189431003421 1104 36-37 10.909986657326563 1104 38-39 10.182695141857135 1104 40-41 10.272581791834796 1104 42-43 10.082149982773714 1104 44-45 9.855602083648513 1104 46-47 9.565458279045263 1104 48-49 9.771136606601843 1104 50-51 11.052906693074938 1104 52-53 10.362090526683478 1104 54-55 10.101311816261521 1104 56-57 9.766619820877192 1104 58-59 9.815080568895976 1104 60-61 9.85343567055116 1104 62-63 9.825376587984298 1104 64-65 9.583505204560566 1104 66-67 9.439361213454877 1104 68-69 9.048599531293995 1104 70-71 8.845174478228223 1104 72-73 8.604276572053752 1104 74-75 8.332315514474812 1104 76-77 7.9510432636032515 1104 78-79 7.95975168232175 1104 80-81 7.391641672658652 1104 82-83 6.923421965860932 1104 84-85 6.887644294540516 1104 86-87 6.985058598194726 1104 88-89 6.690543508285392 1104 90-91 6.154769312220294 1104 92-93 6.032255659262246 1104 94-95 5.87190776520764 1104 96-97 5.27828536824412 1104 98-99 5.169513694206257 1104 100-101 4.614589588989736 1105 1 7.935328597670374 1105 2 9.015973963934787 1105 3 8.490235297121966 1105 4 10.686632221477446 1105 5 10.501957438243519 1105 6 10.406319370115469 1105 7 9.952902131457904 1105 8 9.667203811156394 1105 9 9.542034662659699 1105 10-11 8.93297835860167 1105 12-13 8.548915489408419 1105 14-15 9.160713388713944 1105 16-17 10.109826572691226 1105 18-19 10.452386553362846 1105 20-21 10.772029724114052 1105 22-23 11.364508273854835 1105 24-25 11.424793537213272 1105 26-27 11.345017458515759 1105 28-29 11.231335802078686 1105 30-31 10.964164056960247 1105 32-33 10.716803926387588 1105 34-35 10.824652641950205 1105 36-37 10.765350600736912 1105 38-39 10.672208758506919 1105 40-41 10.445285677061921 1105 42-43 10.056080752760714 1105 44-45 10.202953222477845 1105 46-47 9.762575775509607 1105 48-49 9.77756706453793 1105 50-51 11.19825982240246 1105 52-53 10.0401105075149 1105 54-55 10.020448839932836 1105 56-57 9.983068088727702 1105 58-59 9.636708587275127 1105 60-61 9.680869860947507 1105 62-63 9.473857774536246 1105 64-65 9.253637201174584 1105 66-67 8.684415068471058 1105 68-69 8.453528389209758 1105 70-71 8.586757848473773 1105 72-73 8.441840910691766 1105 74-75 8.063151262290551 1105 76-77 7.997261224995837 1105 78-79 7.884515011498086 1105 80-81 7.758175749906625 1105 82-83 7.457238527056234 1105 84-85 7.174282108924901 1105 86-87 6.906171760189096 1105 88-89 6.624031928159001 1105 90-91 6.1528504292557695 1105 92-93 6.197888047458731 1105 94-95 5.431080533896358 1105 96-97 5.094814161798233 1105 98-99 4.658856649385436 1105 100-101 4.439190099390881 1106 1 7.7082993965269075 1106 2 9.183674583867571 1106 3 8.613534910318165 1106 4 10.45168615039616 1106 5 10.976118732025196 1106 6 10.117822803186773 1106 7 9.514771891774227 1106 8 8.918160024263365 1106 9 9.069329967544466 1106 10-11 8.49567870953948 1106 12-13 8.442241778855067 1106 14-15 9.024211977679727 1106 16-17 10.013698929266582 1106 18-19 11.272130089523277 1106 20-21 11.264777107085688 1106 22-23 11.411673890221149 1106 24-25 10.977429816108732 1106 26-27 11.31070467847277 1106 28-29 11.162552321810661 1106 30-31 10.940066498519716 1106 32-33 11.056630591146277 1106 34-35 10.970274629709653 1106 36-37 10.974848282805343 1106 38-39 10.543310572923804 1106 40-41 10.765003960632402 1106 42-43 10.390876113457363 1106 44-45 9.854039102144498 1106 46-47 9.592756865857883 1106 48-49 9.870122198444367 1106 50-51 11.626797188733 1106 52-53 10.694483249649725 1106 54-55 10.090922413829418 1106 56-57 10.010805679916086 1106 58-59 9.79294599529074 1106 60-61 9.67642595408638 1106 62-63 9.53553191877538 1106 64-65 9.567354517827056 1106 66-67 9.41791826511956 1106 68-69 9.062563582902612 1106 70-71 8.718955339830252 1106 72-73 8.531384581752263 1106 74-75 8.292026281367233 1106 76-77 8.239255147539188 1106 78-79 7.735052617179262 1106 80-81 7.405566516900974 1106 82-83 7.151736719666154 1106 84-85 7.171487854950458 1106 86-87 6.996270439259057 1106 88-89 6.622113829261805 1106 90-91 6.41287360308179 1106 92-93 6.299690204029913 1106 94-95 5.994647341452959 1106 96-97 5.497223149085201 1106 98-99 4.9755684108341 1106 100-101 4.547800939778776 1107 1 7.2440005200475746 1107 2 8.03356037994449 1107 3 8.177924805915175 1107 4 9.920107054709703 1107 5 10.610508074993064 1107 6 9.63433635010334 1107 7 9.589651676636798 1107 8 8.149314181077557 1107 9 5.541428234521433 1107 10-11 7.573366472610161 1107 12-13 7.625931862968148 1107 14-15 8.220749774402236 1107 16-17 9.225351133030827 1107 18-19 10.406601228923794 1107 20-21 10.72078654643061 1107 22-23 10.114659880889402 1107 24-25 10.297443628177493 1107 26-27 10.43476882297907 1107 28-29 10.487854904565044 1107 30-31 10.231295651866251 1107 32-33 10.489999802676241 1107 34-35 10.568436753532982 1107 36-37 10.780511304193556 1107 38-39 10.218903725153371 1107 40-41 9.919815695254766 1107 42-43 10.153109254883212 1107 44-45 9.348799189730727 1107 46-47 9.85050785555812 1107 48-49 9.86458950237905 1107 50-51 11.29225618383363 1107 52-53 10.374858839837518 1107 54-55 10.162656238356124 1107 56-57 9.83267391043783 1107 58-59 9.932039090610484 1107 60-61 9.864314372772856 1107 62-63 9.766459351249406 1107 64-65 9.893418887044806 1107 66-67 9.980048785875542 1107 68-69 9.858319171502057 1107 70-71 9.466078648958913 1107 72-73 8.943963409175694 1107 74-75 8.982799533970356 1107 76-77 9.027885845978249 1107 78-79 8.517019559709125 1107 80-81 8.011662711701652 1107 82-83 7.710119060712994 1107 84-85 7.8455980579849385 1107 86-87 7.485613846301895 1107 88-89 7.523971285345175 1107 90-91 7.080988694871293 1107 92-93 6.694855906767403 1107 94-95 6.120892541173495 1107 96-97 5.900757157553237 1107 98-99 5.7352665948069035 1107 100-101 4.976182215825084 1108 1 6.4627642620052725 1108 2 7.787734005547673 1108 3 7.669641158383346 1108 4 8.835286802714869 1108 5 10.475650373593307 1108 6 9.316476986600662 1108 7 8.540317888573746 1108 8 5.506289438882625 1108 9 6.849244066060887 1108 10-11 5.815960939602231 1108 12-13 4.418220538845649 1108 14-15 6.6029354817792765 1108 16-17 8.241243461513832 1108 18-19 8.051554003195445 1108 20-21 8.101281090587825 1108 22-23 8.925956003148823 1108 24-25 8.612118969800505 1108 26-27 6.916102241117301 1108 28-29 8.855149675794689 1108 30-31 9.374705305953647 1108 32-33 9.740231279842344 1108 34-35 9.2363431435456 1108 36-37 8.947341000968517 1108 38-39 8.747341147362238 1108 40-41 9.310696463587153 1108 42-43 8.486050548326073 1108 44-45 8.086278495690351 1108 46-47 8.441154789001992 1108 48-49 8.971331272557041 1108 50-51 10.519226079190965 1108 52-53 9.879166892172375 1108 54-55 9.185664867620176 1108 56-57 9.092442100631825 1108 58-59 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-11.822883411788753 2313 80-81 -11.4010116363881 2313 82-83 -10.944823328613872 2313 84-85 -10.529477745532345 2313 86-87 -10.057139337445832 2313 88-89 -9.64825430592226 2313 90-91 -9.177349692029859 2313 92-93 -8.653840272735325 2313 94-95 -8.090144450942939 2313 96-97 -7.493724346388547 2313 98-99 -6.867219760559985 2313 100-101 -6.002289585266487 2314 1 -18.463094689170134 2314 2 -17.249035132487286 2314 3 -17.84633231961545 2314 4 -19.35157275123329 2314 5 -18.662384587226462 2314 6 -19.598532054990656 2314 7 -19.7948237630236 2314 8 -20.22849054906192 2314 9 -21.491925313083176 2314 10-11 -21.830211453405607 2314 12-13 -21.73337680895423 2314 14-15 -21.674641371746944 2314 16-17 -20.48811157163867 2314 18-19 -19.44573351940853 2314 20-21 -19.10516857788716 2314 22-23 -18.741313436272517 2314 24-25 -18.547917196564775 2314 26-27 -18.262017107888123 2314 28-29 -18.077942548436777 2314 30-31 -18.0270427350349 2314 32-33 -17.896598135468086 2314 34-35 -17.575893143374234 2314 36-37 -17.0924420008398 2314 38-39 -16.963327930395447 2314 40-41 -16.833064838402 2314 42-43 -16.661930162945776 2314 44-45 -16.25338395156703 2314 46-47 -15.911165886103493 2314 48-49 -15.676950825092145 2314 50-51 -13.603438176384685 2314 52-53 -15.36043527780954 2314 54-55 -16.07021216673787 2314 56-57 -16.021783336378423 2314 58-59 -15.752618277491397 2314 60-61 -15.427074347663773 2314 62-63 -15.00188509993313 2314 64-65 -14.642663587181996 2314 66-67 -14.263735325707234 2314 68-69 -13.837557117157736 2314 70-71 -13.469336754315798 2314 72-73 -13.07392622090314 2314 74-75 -12.593610411451111 2314 76-77 -12.192247568212169 2314 78-79 -11.822883411788753 2314 80-81 -11.4010116363881 2314 82-83 -10.944823328613872 2314 84-85 -10.529477745532345 2314 86-87 -10.057139337445832 2314 88-89 -9.64825430592226 2314 90-91 -9.177349692029859 2314 92-93 -8.653840272735325 2314 94-95 -8.090144450942939 2314 96-97 -7.493724346388547 2314 98-99 -6.867219760559985 2314 100-101 -6.002289585266487 2315 1 -18.463094689170134 2315 2 -17.249035132487286 2315 3 -17.84633231961545 2315 4 -19.35157275123329 2315 5 -18.662384587226462 2315 6 -19.598532054990656 2315 7 -19.7948237630236 2315 8 -20.22849054906192 2315 9 -21.491925313083176 2315 10-11 -21.830211453405607 2315 12-13 -21.73337680895423 2315 14-15 -21.674641371746944 2315 16-17 -20.48811157163867 2315 18-19 -19.44573351940853 2315 20-21 -19.10516857788716 2315 22-23 -18.741313436272517 2315 24-25 -18.547917196564775 2315 26-27 -18.262017107888123 2315 28-29 -18.077942548436777 2315 30-31 -18.0270427350349 2315 32-33 -17.896598135468086 2315 34-35 -17.575893143374234 2315 36-37 -17.0924420008398 2315 38-39 -16.963327930395447 2315 40-41 -16.833064838402 2315 42-43 -16.661930162945776 2315 44-45 -16.25338395156703 2315 46-47 -15.911165886103493 2315 48-49 -15.676950825092145 2315 50-51 -13.603438176384685 2315 52-53 -15.36043527780954 2315 54-55 -16.07021216673787 2315 56-57 -16.021783336378423 2315 58-59 -15.752618277491397 2315 60-61 -15.427074347663773 2315 62-63 -15.00188509993313 2315 64-65 -14.642663587181996 2315 66-67 -14.263735325707234 2315 68-69 -13.837557117157736 2315 70-71 -13.469336754315798 2315 72-73 -13.07392622090314 2315 74-75 -12.593610411451111 2315 76-77 -12.192247568212169 2315 78-79 -11.822883411788753 2315 80-81 -11.4010116363881 2315 82-83 -10.944823328613872 2315 84-85 -10.529477745532345 2315 86-87 -10.057139337445832 2315 88-89 -9.64825430592226 2315 90-91 -9.177349692029859 2315 92-93 -8.653840272735325 2315 94-95 -8.090144450942939 2315 96-97 -7.493724346388547 2315 98-99 -6.867219760559985 2315 100-101 -6.002289585266487 2316 1 -18.463094689170134 2316 2 -17.249035132487286 2316 3 -17.84633231961545 2316 4 -19.35157275123329 2316 5 -18.662384587226462 2316 6 -19.598532054990656 2316 7 -19.7948237630236 2316 8 -20.22849054906192 2316 9 -21.491925313083176 2316 10-11 -21.830211453405607 2316 12-13 -21.73337680895423 2316 14-15 -21.674641371746944 2316 16-17 -20.48811157163867 2316 18-19 -19.44573351940853 2316 20-21 -19.10516857788716 2316 22-23 -18.741313436272517 2316 24-25 -18.547917196564775 2316 26-27 -18.262017107888123 2316 28-29 -18.077942548436777 2316 30-31 -18.0270427350349 2316 32-33 -17.896598135468086 2316 34-35 -17.575893143374234 2316 36-37 -17.0924420008398 2316 38-39 -16.963327930395447 2316 40-41 -16.833064838402 2316 42-43 -16.661930162945776 2316 44-45 -16.25338395156703 2316 46-47 -15.911165886103493 2316 48-49 -15.676950825092145 2316 50-51 -13.603438176384685 2316 52-53 -15.36043527780954 2316 54-55 -16.07021216673787 2316 56-57 -16.021783336378423 2316 58-59 -15.752618277491397 2316 60-61 -15.427074347663773 2316 62-63 -15.00188509993313 2316 64-65 -14.642663587181996 2316 66-67 -14.263735325707234 2316 68-69 -13.837557117157736 2316 70-71 -13.469336754315798 2316 72-73 -13.07392622090314 2316 74-75 -12.593610411451111 2316 76-77 -12.192247568212169 2316 78-79 -11.822883411788753 2316 80-81 -11.4010116363881 2316 82-83 -10.944823328613872 2316 84-85 -10.529477745532345 2316 86-87 -10.057139337445832 2316 88-89 -9.64825430592226 2316 90-91 -9.177349692029859 2316 92-93 -8.653840272735325 2316 94-95 -8.090144450942939 2316 96-97 -7.493724346388547 2316 98-99 -6.867219760559985 2316 100-101 -6.002289585266487 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 393153.0 3 17312.0 4 57915.0 5 32440.0 6 19592.0 7 19576.0 8 19055.0 9 19112.0 10 20272.0 11 21320.0 12 21880.0 13 22259.0 14 22688.0 15 23140.0 16 24336.0 17 24313.0 18 23793.0 19 24323.0 20 26179.0 21 27420.0 22 28692.0 23 30326.0 24 32688.0 25 34721.0 26 36323.0 27 38833.0 28 41479.0 29 44667.0 30 48026.0 31 51656.0 32 55598.0 33 58520.0 34 59197.0 35 54680.0 36 38598.0 37 15271.0 38 1647.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.43365645825596 21.13186918656813 14.550135829309921 22.884338525865992 2 14.704660728531593 21.128806786161267 41.761295020895695 22.405237464411442 3 18.43032759618227 26.571175679442682 30.564498946022724 24.433997778352325 4 12.127246053570822 17.68986248548753 40.06971482713784 30.11317663380381 5 12.483787320272574 38.058613563420174 36.20115496498062 13.25644415132664 6 29.00907401447274 39.30965506978547 18.33265849244005 13.348612423301736 7 25.63703342228653 33.229990649676836 23.537220428749855 17.595755499286785 8 24.02217681399814 37.215628291667954 20.962883375342255 17.799311518991658 9 24.887679255642606 17.184778694186477 21.8830624450204 36.04447960515052 10-11 23.52723574187619 27.58405481948907 29.438980825099453 19.449728613535292 12-13 24.23702918817688 25.076081465692496 29.686826201594325 21.000063144536306 14-15 21.634322471023726 26.72504399538807 27.251896352934036 24.388737180654164 16-17 20.990656150465423 29.799596522290056 27.940557597938547 21.26918972930597 18-19 20.693981120022713 28.612215203350132 30.274549293775287 20.41925438285187 20-21 21.430875774750845 27.87683931314969 30.271260331342166 20.421024580757294 22-23 21.180752232957754 27.797064445689067 29.948096061967274 21.074087259385905 24-25 21.03226328462039 28.05276859989117 30.10534514583652 20.80962296965192 26-27 20.98466582121857 28.18285808627562 29.97821066171735 20.854265430788463 28-29 20.99882648726199 28.1487227166994 30.112833075648044 20.73961772039057 30-31 21.11573747290589 27.954964658304114 30.108572759454272 20.820725109335722 32-33 21.01004781275788 28.154395841021042 29.918421518471188 20.917134827749894 34-35 21.26346587271565 28.04783114056463 29.926022234120804 20.762680752598925 36-37 21.277671252084247 27.996783092749993 29.810647230664394 20.914898424501366 38-39 21.18551440447004 28.117763440937154 29.921083772129503 20.7756383824633 40-41 21.341850479444275 28.0940670705903 29.828332455986427 20.73574999397899 42-43 21.182598105842313 28.06567573151239 29.60440157882719 21.147324583818108 44-45 21.239110715569325 28.300715398175562 29.529428879494773 20.930745006760343 46-47 21.40022856754957 28.22724384066725 29.221990538428866 21.150537053354313 48-49 21.366809397440097 28.348745257316963 29.131584933920962 21.15286041132197 50-51 21.57293298236454 28.632146275670035 28.703391489731988 21.09152925223344 52-53 21.153135533269527 28.778744122175524 28.83146768757022 21.236652656984727 54-55 21.06706028539328 28.766818481784085 28.915903263356512 21.25021796946612 56-57 21.062287429721664 28.808435135697923 29.006082112861804 21.12319532171861 58-59 21.038027459047566 28.7740290301496 28.993278553898556 21.194664956904276 60-61 20.99005297047518 28.896219068858198 29.003536208743164 21.11019175192346 62-63 20.98200884207079 28.70581523073984 29.039226455257484 21.272949471931884 64-65 20.895783472612052 28.681860734114423 28.9565960110865 21.46575978218703 66-67 20.797847139310555 28.737952802993455 28.807693360538888 21.656506697157106 68-69 20.885571999264744 28.786612912924824 28.857323563492663 21.470491524317765 70-71 20.995918333190374 28.625559895479203 28.87192436677678 21.50659740455364 72-73 21.026231780121353 28.72931892102314 28.73767259206477 21.506776706790742 74-75 20.950793910491413 28.918338615297294 28.49832653646575 21.63254093774554 76-77 21.014865066367168 28.817976142957836 28.542514073195985 21.624644717479008 78-79 20.796286978754868 28.9187673919517 28.547013461755675 21.737932167537764 80-81 20.94864801326501 28.971108656168663 28.287178334924885 21.793064995641437 82-83 21.10303905895887 28.761149761468474 28.46778920423058 21.66802197534207 84-85 21.075515115091438 28.870257980750463 28.294780528856084 21.759446375302012 86-87 21.02934968002539 28.870388719527924 28.424235946352717 21.67602565409397 88-89 21.160222253140436 28.769967674193627 28.26539055806563 21.804419514600312 90-91 20.98280787516888 28.805585635757968 28.395202293658407 21.816404195414744 92-93 21.072251148204025 28.8271920455544 28.188207687861773 21.912349118379804 94-95 20.920864670622542 28.709694125764564 28.292153589996317 22.077287613616576 96-97 20.947515132592248 28.700866351970976 28.207739389481784 22.14387912595499 98-99 20.87394807648843 28.71602437803038 28.003108350617477 22.406919194863715 100-101 21.15982624454161 28.807544674938136 27.901715747515727 22.13091333300453 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 89178.0 1 81176.0 2 54241.5 3 27577.0 4 17108.0 5 13443.0 6 12401.0 7 12506.0 8 13050.0 9 13033.0 10 11903.0 11 10219.0 12 8195.5 13 6233.0 14 4715.0 15 3816.5 16 3593.0 17 3793.0 18 4375.0 19 5059.5 20 5878.5 21 6831.5 22 7763.0 23 8892.5 24 10206.0 25 11731.0 26 13495.0 27 15378.0 28 17487.5 29 20030.5 30 22686.0 31 25361.0 32 28470.5 33 31372.5 34 34049.0 35 36733.0 36 39272.5 37 41734.5 38 43548.5 39 44320.5 40 44560.5 41 44366.5 42 44269.5 43 44686.0 44 45034.5 45 45202.5 46 44839.5 47 44023.0 48 42861.0 49 41287.5 50 39172.5 51 36600.0 52 33588.5 53 30352.5 54 26898.5 55 23182.5 56 19378.5 57 15700.0 58 12655.0 59 10017.5 60 7706.5 61 5768.5 62 4196.0 63 2875.5 64 1883.5 65 1272.5 66 825.0 67 532.0 68 344.0 69 211.5 70 132.0 71 94.5 72 65.5 73 42.5 74 34.5 75 27.0 76 16.5 77 10.5 78 8.5 79 6.0 80 3.0 81 2.0 82 1.5 83 1.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.738264283054974 2 28.606083565750712 3 22.442897172454785 4 28.720060613581882 5 31.76354171429318 6 24.505522498220127 7 23.439298241030432 8 19.512086792378888 9 11.493197301128014 10-11 15.071260148529403 12-13 15.179652647598644 14-15 19.27387802822973 16-17 22.47402041789407 18-19 26.380624555032302 20-21 28.42923952162298 22-23 27.987570296732223 24-25 26.539735207477417 26-27 27.99694319436189 28-29 24.97188130711099 30-31 26.804462413700758 32-33 25.835167634437394 34-35 23.940960542387053 36-37 26.864684097425883 38-39 26.974938112749104 40-41 24.06346137517064 42-43 24.913129241481112 44-45 23.67260827819544 46-47 26.873011933298933 48-49 22.334374040665967 50-51 27.940901757663244 52-53 21.5191278959641 54-55 22.650701171122332 56-57 24.718976362009393 58-59 24.003468298704778 60-61 23.586586632353807 62-63 23.41160410448005 64-65 21.960895095394545 66-67 22.452661968243188 68-69 23.42417750373936 70-71 21.948419670674916 72-73 21.81118999875899 74-75 19.15777819870543 76-77 21.37246001005872 78-79 20.48794586580101 80-81 21.512106387286824 82-83 22.328985440983402 84-85 19.35865866323538 86-87 20.86397214910419 88-89 19.722993318136396 90-91 20.83412257268078 92-93 20.83647396163317 94-95 19.915056074094878 96-97 18.832535385137916 98-99 18.216667428690865 100-101 19.87860954533282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1531010.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.9013234283069 #Duplication Level Percentage of deduplicated Percentage of total 1 91.30393845776149 73.8660945544959 2 5.846907180354851 9.460450577063554 3 1.3843645099435908 3.3599076288484806 4 0.539588871550903 1.7461381526261897 5 0.2832713200332764 1.145851233998776 6 0.1687237558163359 0.818998508360164 7 0.10874682651525892 0.6158433528599059 8 0.07380277147954514 0.47765935082976835 9 0.051236407174760815 0.3730583833334773 >10 0.23072030524069437 3.0362450646511188 >50 0.006745760946613754 0.35791772966829855 >100 0.0016282270088500746 0.20950189423862975 >500 8.140154346636729E-5 0.037010468386912104 >1k 1.6280308693273458E-4 0.15331026762407718 >5k 0.0 0.0 >10k+ 8.140154346636729E-5 4.342012833014725 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 65933 4.306503549944154 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 6.531635978863626E-5 0.0 0.0 0.0 10-11 0.0 6.531635978863626E-5 0.0 0.0 0.0 12-13 0.0 6.531635978863626E-5 0.0 0.0 0.0 14-15 0.0 6.531635978863626E-5 0.0 0.0 0.0 16-17 0.0 6.531635978863626E-5 0.0 0.0 0.0 18-19 0.0 6.531635978863626E-5 0.0 0.0 0.0 20-21 0.0 6.531635978863626E-5 0.0 0.0 0.0 22-23 0.0 6.531635978863626E-5 0.0 0.0 0.0 24-25 0.0 1.3063271957727252E-4 0.0 6.531635978863626E-5 0.0 26-27 0.0 1.3063271957727252E-4 0.0 1.959490793659088E-4 0.0 28-29 0.0 1.3063271957727252E-4 0.0 5.551890582034082E-4 0.0 30-31 0.0 1.3063271957727252E-4 0.0 0.001273669015878407 0.0 32-33 0.0 1.3063271957727252E-4 0.0 0.003004552550277268 0.0 34-35 0.0 1.3063271957727252E-4 0.0 0.006041763280448854 0.0 36-37 0.0 1.3063271957727252E-4 0.0 0.011822261121743163 0.0 38-39 0.0 1.3063271957727252E-4 0.0 0.02044402061384315 0.0 40-41 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.027759452910170408 0.0 42-43 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.0342257725292454 0.0 44-45 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.04464373191553288 0.0 46-47 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.05519232402139764 0.0 48-49 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.0691700250161658 0.0 50-51 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.08598898766173962 0.0 52-53 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.10522465561949301 0.0 54-55 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.12527677807460436 0.0 56-57 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.1508154747519611 0.0 58-59 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.17870556038170882 0.0 60-61 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.20721615142944855 0.0 62-63 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.23837205504862802 0.0 64-65 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.26851555509108366 0.0 66-67 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.3065949928478586 0.0 68-69 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.35114075022370855 0.0 70-71 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.3972214420545914 0.0 72-73 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.44973579532465496 0.0 74-75 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.5030666030920765 0.0 76-77 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.5609042396849139 0.0 78-79 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.6238038941613706 0.0 80-81 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.6933984755161625 0.0 82-83 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.7686755801725659 0.0 84-85 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.8455202774638964 0.0 86-87 0.0 1.3063271957727252E-4 6.531635978863626E-5 0.9289945852737735 0.0 88-89 0.0 1.3063271957727252E-4 6.531635978863626E-5 1.020698754417019 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1180 0.0 53.730225 1 GTATCAA 2365 0.0 47.13075 1 ATCAACG 2915 0.0 38.47581 3 TCAACGC 2960 0.0 37.719128 4 CAACGCA 3100 0.0 36.010445 5 TATCAAC 3100 0.0 35.94911 2 AACGCAG 3355 0.0 34.050957 6 ACGCAGA 3755 0.0 28.979815 7 CGCAGAG 3830 0.0 28.438795 8 GCAGAGT 4090 0.0 25.094213 9 GTACATG 2370 0.0 22.868437 1 AGAGTAC 3590 0.0 22.355291 10-11 TACATGG 2415 0.0 21.802856 2 GAGTACT 2680 0.0 21.41199 12-13 CGTAGAC 120 0.0034208167 20.956089 3 CAGAGTA 3965 0.0 20.645803 10-11 ACATGGG 2420 0.0 20.575068 3 ACGCGAC 60 0.0048130527 19.757256 54-55 GAGTACA 1820 0.0 19.384592 1 TAACGCA 210 1.1954851E-5 19.158922 4 >>END_MODULE