FastQCFastQC Report
Sat 18 Jun 2016
SRR1931025_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931025_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1531010
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.2719120058000928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37660.24598141096400417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23400.15284028190540885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.056.7853551
TAGGCCG802.284396E-741.5133325
GTATCAA43100.038.036691
ATCAACG53100.030.2421073
TCAACGC54250.029.6000634
CAACGCA54050.029.5810625
TATCAAC55800.028.8667562
AACGCAG57200.028.2838126
GGTCCGC700.00844101627.1107486
TGGTATC12100.026.3100662
GTGGTAT13150.025.656271
ACGCAGA64550.024.6222727
CGCAGAG65450.024.1387168
TGGACCG1801.9743493E-723.7219055
GCAGAGT72250.021.4728419
GTACATG36850.021.2768521
GAGTACT51400.020.49129712-13
CAGAGTA70100.019.79645510-11
GGACCGT1200.00479693919.7682536
AGAGTAC66250.019.62204410-11
Produced by FastQC (version 0.11.5)