FastQCFastQC Report
Sat 18 Jun 2016
SRR1931024_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931024_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3420139
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1546224.520927365817588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23600.052.6027871
GTATCAA46900.046.1577871
ATCAACG55700.036.1211173
TATCAAC59500.034.997442
CAACGCA57750.034.9153945
TCAACGC57650.034.897014
AACGCAG64100.032.6926966
ACGCAGA74850.026.9363967
CGCAGAG75850.026.617198
GTACATG58200.026.5307121
TACATGG59800.025.2158342
ACATGGG58100.024.9085923
GCAGAGT81450.023.3538659
GTGTAGC13300.021.985111
GAGTACT50750.020.58674212-13
CATGGGG35450.020.1969934
GGCGTCG2902.728484E-1019.8471578
AGAGTAC73150.019.5135710-11
CAGAGTA80400.018.89202510-11
GAGTACA42000.018.4430641
Produced by FastQC (version 0.11.5)