##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931024_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3420139 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.015540304063666 28.0 2.0 31.0 2.0 33.0 2 19.700465390441735 27.0 2.0 31.0 2.0 33.0 3 20.37326728533548 27.0 2.0 31.0 2.0 33.0 4 21.851699887051375 30.0 2.0 35.0 2.0 37.0 5 21.17302366950583 30.0 2.0 35.0 2.0 37.0 6 22.184224091476985 30.0 2.0 35.0 2.0 37.0 7 22.38802194881553 30.0 2.0 35.0 2.0 37.0 8 22.83445088050515 32.0 2.0 35.0 2.0 37.0 9 24.159437087206104 32.0 2.0 37.0 2.0 39.0 10-11 24.536169582581294 32.0 2.0 37.0 2.0 39.0 12-13 24.451446271628143 32.0 2.0 37.0 2.0 39.0 14-15 24.398736279431915 32.0 2.0 38.0 2.0 40.0 16-17 23.205167246126546 31.5 2.0 38.0 2.0 40.0 18-19 22.124268925912077 28.5 2.0 37.5 2.0 40.0 20-21 21.76901494354469 27.5 2.0 37.0 2.0 40.0 22-23 21.400199231668655 27.0 2.0 37.0 2.0 40.0 24-25 21.24697768131646 27.0 2.0 37.0 2.0 39.5 26-27 20.966167310743803 27.0 2.0 37.0 2.0 39.0 28-29 20.77329868756796 26.0 2.0 37.0 2.0 39.0 30-31 20.773397806346466 26.0 2.0 36.0 2.0 39.0 32-33 20.658279239527985 25.5 2.0 36.5 2.0 39.0 34-35 20.334337434823556 25.0 2.0 36.0 2.0 39.0 36-37 19.86167535880852 24.0 2.0 36.0 2.0 39.0 38-39 19.756828304346694 24.0 2.0 35.5 2.0 39.0 40-41 19.62981753080796 24.0 2.0 35.0 2.0 39.0 42-43 19.499422099511158 24.0 2.0 35.0 2.0 39.0 44-45 19.122474992975434 23.0 2.0 35.0 2.0 38.0 46-47 18.799547328339578 20.0 2.0 35.0 2.0 38.0 48-49 18.574807924473248 18.5 2.0 35.0 2.0 38.0 50-51 16.340598876244503 11.0 2.0 33.0 2.0 37.5 52-53 18.252648649660145 19.5 2.0 34.0 2.0 38.0 54-55 19.012738078773992 22.0 2.0 35.5 2.0 39.0 56-57 18.994930907778894 22.0 2.0 36.0 2.0 39.0 58-59 18.716029962524914 20.5 2.0 35.5 2.0 39.0 60-61 18.42874850992898 19.0 2.0 35.0 2.0 39.0 62-63 17.994433267185926 16.0 2.0 34.5 2.0 38.5 64-65 17.647429388103816 13.0 2.0 34.0 2.0 38.0 66-67 17.293532221936008 9.5 2.0 34.0 2.0 38.0 68-69 16.848194766353064 8.0 2.0 33.5 2.0 38.0 70-71 16.504357863817816 7.0 2.0 33.0 2.0 37.0 72-73 16.100571643433206 2.0 2.0 32.0 2.0 36.5 74-75 15.65446769853506 2.0 2.0 32.0 2.0 36.0 76-77 15.245250997108597 2.0 2.0 31.0 2.0 36.0 78-79 14.86494583992054 2.0 2.0 31.0 2.0 35.0 80-81 14.42504178923722 2.0 2.0 31.0 2.0 35.0 82-83 13.958621126217384 2.0 2.0 30.0 2.0 34.0 84-85 13.554543689598582 2.0 2.0 30.0 2.0 34.0 86-87 13.067494040446894 2.0 2.0 29.0 2.0 34.0 88-89 12.64489206432838 2.0 2.0 29.0 2.0 34.0 90-91 12.153561887396975 2.0 2.0 29.0 2.0 34.0 92-93 11.60515084328444 2.0 2.0 27.5 2.0 34.0 94-95 11.031093180715754 2.0 2.0 26.0 2.0 34.0 96-97 10.389394115268415 2.0 2.0 25.0 2.0 33.0 98-99 9.707839213552433 2.0 2.0 22.0 2.0 32.5 100-101 8.775584120996253 2.0 2.0 10.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.75438516421136 1101 2 9.497844597292996 1101 3 8.708699820593807 1101 4 10.899411511190149 1101 5 11.641419862671032 1101 6 10.738552083081753 1101 7 10.426985125140721 1101 8 9.953594451939434 1101 9 9.645648041409 1101 10-11 9.053929479469769 1101 12-13 8.998639406185315 1101 14-15 9.71628135061722 1101 16-17 10.770356774462652 1101 18-19 11.581286027722289 1101 20-21 11.757534908667203 1101 22-23 11.764327395466491 1101 24-25 11.800012216904936 1101 26-27 11.871104804477273 1101 28-29 11.882135810349858 1101 30-31 11.529512022217368 1101 32-33 11.52509889104865 1101 34-35 11.274790994138446 1101 36-37 11.586340316778458 1101 38-39 11.287795256469373 1101 40-41 11.000191814678281 1101 42-43 10.78338179967757 1101 44-45 10.56353739945525 1101 46-47 10.29050991411857 1101 48-49 10.43443733940601 1101 50-51 12.228585299078198 1101 52-53 11.52810517820516 1101 54-55 11.184536475863545 1101 56-57 11.057211633957735 1101 58-59 10.715828821986054 1101 60-61 10.61473703053025 1101 62-63 10.863525599374281 1101 64-65 10.48926900139524 1101 66-67 10.224912324556481 1101 68-69 10.32430558523918 1101 70-71 10.062757129545265 1101 72-73 9.899938101582256 1101 74-75 9.726168898271451 1101 76-77 9.392589188810222 1101 78-79 9.178928766786528 1101 80-81 8.939291089875445 1101 82-83 8.493321851403374 1101 84-85 8.28054350822412 1101 86-87 8.045336236862415 1101 88-89 7.831091010242346 1101 90-91 7.441180923716628 1101 92-93 7.114288969607408 1101 94-95 6.999287062476574 1101 96-97 6.7664669924784615 1101 98-99 6.263605916390091 1101 100-101 5.530123924560639 1102 1 7.9315708841464065 1102 2 9.010481428283061 1102 3 8.551410862150039 1102 4 10.487125960374417 1102 5 11.456096642841164 1102 6 10.401100147464884 1102 7 10.166880035574795 1102 8 9.49279269175231 1102 9 9.358503633705237 1102 10-11 8.572985272016734 1102 12-13 8.89850562523269 1102 14-15 9.33078627695355 1102 16-17 10.270667036892082 1102 18-19 11.291691703736799 1102 20-21 11.296935713035701 1102 22-23 11.408455337175017 1102 24-25 11.214999890546725 1102 26-27 11.492662258768778 1102 28-29 11.107291046738279 1102 30-31 11.136311291891161 1102 32-33 11.156861980967964 1102 34-35 10.942173473040604 1102 36-37 11.004239619266446 1102 38-39 10.92322049479299 1102 40-41 10.548647654343075 1102 42-43 10.205735418915104 1102 44-45 9.96695091722119 1102 46-47 9.923206525842492 1102 48-49 9.565492820638813 1102 50-51 11.481369036699252 1102 52-53 10.748799156379512 1102 54-55 10.30696455808414 1102 56-57 10.314379852988285 1102 58-59 10.244370651671964 1102 60-61 10.124051844877627 1102 62-63 9.981799320251682 1102 64-65 9.640781399271575 1102 66-67 9.649216705672334 1102 68-69 9.34751300461302 1102 70-71 9.146254113163408 1102 72-73 8.99320782585887 1102 74-75 8.689441819828692 1102 76-77 8.443502937958316 1102 78-79 8.101869255297354 1102 80-81 7.903451046459738 1102 82-83 7.780849722435169 1102 84-85 7.5652895347615505 1102 86-87 7.4621087082762045 1102 88-89 7.125661312553646 1102 90-91 7.047521425689686 1102 92-93 6.670654082227408 1102 94-95 6.409473977184907 1102 96-97 6.15692479258111 1102 98-99 5.778981049960699 1102 100-101 5.214770036078422 1103 1 7.923072488332789 1103 2 9.320210414760414 1103 3 8.694694052980623 1103 4 10.84026640664489 1103 5 11.31249228499384 1103 6 10.556025642652326 1103 7 10.094270734920922 1103 8 9.5902680354241 1103 9 9.468741079669048 1103 10-11 8.909842154850129 1103 12-13 8.836815508537608 1103 14-15 9.37563993822457 1103 16-17 10.170171025100569 1103 18-19 11.149743054188054 1103 20-21 11.591330705600058 1103 22-23 11.78097280516548 1103 24-25 11.511636539858056 1103 26-27 11.552574333527499 1103 28-29 11.177328455771182 1103 30-31 11.006828720312793 1103 32-33 11.296387204449424 1103 34-35 11.139191219661587 1103 36-37 11.122234699541718 1103 38-39 10.898383551353337 1103 40-41 10.681248991769582 1103 42-43 10.267111293138974 1103 44-45 10.460275160714591 1103 46-47 10.41019901435929 1103 48-49 10.28604132708029 1103 50-51 11.892508322283998 1103 52-53 11.093520839110024 1103 54-55 10.596672045496305 1103 56-57 10.476052988059788 1103 58-59 10.330324952968834 1103 60-61 10.083576474826614 1103 62-63 10.171005602463186 1103 64-65 9.829303635306246 1103 66-67 9.717862858098066 1103 68-69 9.406594628517865 1103 70-71 9.203057973762412 1103 72-73 9.162096007638086 1103 74-75 8.951732228673647 1103 76-77 8.607089720649588 1103 78-79 8.136522154208881 1103 80-81 7.974950358951288 1103 82-83 7.765262487999733 1103 84-85 7.428600654592458 1103 86-87 7.282242401466078 1103 88-89 6.912416058340391 1103 90-91 6.759442278134218 1103 92-93 6.358597624099474 1103 94-95 6.1088023787030306 1103 96-97 5.735740959086753 1103 98-99 5.335837009521352 1103 100-101 4.592761274879619 1104 1 7.832279053996416 1104 2 9.392389219617264 1104 3 8.765526456056651 1104 4 10.861768088662611 1104 5 11.434489563097134 1104 6 10.590571902921521 1104 7 10.274200098052706 1104 8 9.794804412061051 1104 9 9.581909070716716 1104 10-11 8.774942348584748 1104 12-13 8.746041282105832 1104 14-15 9.34673300622843 1104 16-17 10.435387824478585 1104 18-19 11.339673346371622 1104 20-21 11.505979577871031 1104 22-23 11.623835147819396 1104 24-25 11.520381199618559 1104 26-27 11.601373863600887 1104 28-29 11.227086291511291 1104 30-31 10.90376284137917 1104 32-33 11.159031076878719 1104 34-35 10.960131596879116 1104 36-37 11.07677368813222 1104 38-39 10.844009750742313 1104 40-41 10.566767429188058 1104 42-43 10.224178210746068 1104 44-45 10.301389503682902 1104 46-47 10.002522892742231 1104 48-49 10.044333355381973 1104 50-51 11.54125385323147 1104 52-53 10.81381983304981 1104 54-55 10.502485562942628 1104 56-57 10.207050262466161 1104 58-59 10.159886745923053 1104 60-61 10.063298714060021 1104 62-63 10.107525446800398 1104 64-65 9.776691376355402 1104 66-67 9.642383387811893 1104 68-69 9.396663257798398 1104 70-71 9.095628926877929 1104 72-73 9.032694484153888 1104 74-75 8.733639973831204 1104 76-77 8.422867230106066 1104 78-79 8.295876034052908 1104 80-81 8.036876362066023 1104 82-83 7.915142008628299 1104 84-85 7.603775096924302 1104 86-87 7.095674175712279 1104 88-89 6.796194532853431 1104 90-91 6.33656494849266 1104 92-93 6.218100240633994 1104 94-95 5.657772569803198 1104 96-97 5.367426977516375 1104 98-99 4.931546944122857 1104 100-101 4.272184361044994 1105 1 7.996944266338577 1105 2 9.16093619378308 1105 3 8.597778188282785 1105 4 10.732912687056665 1105 5 10.913717852193592 1105 6 10.565431592487474 1105 7 10.006754650458785 1105 8 9.53559146322824 1105 9 9.452826518813229 1105 10-11 8.85732992559667 1105 12-13 8.56056228096703 1105 14-15 9.32372987769605 1105 16-17 10.347601032576858 1105 18-19 10.83822581996569 1105 20-21 11.25326291657251 1105 22-23 11.681434860705163 1105 24-25 11.685448789138977 1105 26-27 11.520740782359841 1105 28-29 11.433465229779387 1105 30-31 11.244036214066469 1105 32-33 11.017400053352137 1105 34-35 11.1899769893381 1105 36-37 11.200475661315824 1105 38-39 11.170354285937485 1105 40-41 10.803690229498802 1105 42-43 10.577982380466018 1105 44-45 10.371692051938858 1105 46-47 10.311350483087804 1105 48-49 10.194152103978396 1105 50-51 11.64587344329199 1105 52-53 10.658929350436303 1105 54-55 10.414944674716985 1105 56-57 10.455344173853636 1105 58-59 10.345457873033919 1105 60-61 10.207823032269225 1105 62-63 10.116557542738931 1105 64-65 9.975290358328252 1105 66-67 9.912421255571505 1105 68-69 9.653672215818492 1105 70-71 9.622516700565907 1105 72-73 9.435567220445288 1105 74-75 9.295087997201668 1105 76-77 8.962801377617327 1105 78-79 8.678739853112814 1105 80-81 8.396860473266058 1105 82-83 8.103779313130188 1105 84-85 7.906298739074209 1105 86-87 7.566335687075188 1105 88-89 7.122682414185803 1105 90-91 6.76496818270032 1105 92-93 6.492863373742193 1105 94-95 6.252600317677148 1105 96-97 6.097362855026811 1105 98-99 5.561174984924582 1105 100-101 4.97690948574324 1106 1 7.82638520609925 1106 2 9.174385769461075 1106 3 8.736344053880337 1106 4 10.461194211331676 1106 5 11.215862773911407 1106 6 10.349537342573182 1106 7 9.636409738624273 1106 8 9.205968971537732 1106 9 8.902161406464348 1106 10-11 8.37365847289578 1106 12-13 8.250362085817894 1106 14-15 8.743027129002908 1106 16-17 9.909257249648508 1106 18-19 11.133313159795609 1106 20-21 11.13495045020106 1106 22-23 11.321042386255183 1106 24-25 11.042159043615552 1106 26-27 11.228369815444648 1106 28-29 11.173205473933741 1106 30-31 10.856513236160556 1106 32-33 11.017867591775005 1106 34-35 10.9388326596975 1106 36-37 11.098091882942391 1106 38-39 10.835662075543507 1106 40-41 10.771023466454611 1106 42-43 10.48343150126665 1106 44-45 10.018198474088994 1106 46-47 9.762887551142306 1106 48-49 9.861885062114641 1106 50-51 11.622913799999328 1106 52-53 10.940851259472488 1106 54-55 10.548278683306862 1106 56-57 10.63498213505769 1106 58-59 10.200644503199904 1106 60-61 10.236497264264656 1106 62-63 10.231883610311854 1106 64-65 10.189232349494052 1106 66-67 9.753453280840834 1106 68-69 9.347817296528529 1106 70-71 9.04230911442696 1106 72-73 9.077305794260498 1106 74-75 8.726422924140442 1106 76-77 8.468288374171374 1106 78-79 8.071960998710095 1106 80-81 7.688160237754991 1106 82-83 7.67429597032792 1106 84-85 7.520893304302611 1106 86-87 7.133945671786762 1106 88-89 6.676831696833151 1106 90-91 6.701849728276608 1106 92-93 6.436836494234662 1106 94-95 6.11827061262 1106 96-97 5.578881313624835 1106 98-99 5.243472382639329 1106 100-101 4.6514988300108175 1107 1 7.393726316852959 1107 2 8.331383969240317 1107 3 8.174597175902473 1107 4 10.021032390616288 1107 5 10.891464864171084 1107 6 9.783295796761095 1107 7 9.722661012749676 1107 8 8.456329860580041 1107 9 5.937817304193949 1107 10-11 7.76026425412098 1107 12-13 7.684780610892137 1107 14-15 8.272819862218395 1107 16-17 9.404927410944186 1107 18-19 10.72551555759551 1107 20-21 10.982950835229836 1107 22-23 10.295822096083494 1107 24-25 10.696672661141964 1107 26-27 10.671641211246389 1107 28-29 10.829972762057775 1107 30-31 10.638137270043089 1107 32-33 10.858449685281474 1107 34-35 10.839124765581623 1107 36-37 11.203920029753018 1107 38-39 10.809912144492625 1107 40-41 10.440291365615696 1107 42-43 10.273769376088847 1107 44-45 9.632167822056637 1107 46-47 9.885710071584136 1107 48-49 10.048895440390325 1107 50-51 11.513085774490872 1107 52-53 10.622386217731872 1107 54-55 10.342694287473137 1107 56-57 10.1476192110357 1107 58-59 10.251808672108044 1107 60-61 10.013711043949744 1107 62-63 10.087995700758615 1107 64-65 10.038115947087078 1107 66-67 9.998856773498428 1107 68-69 9.872548304440933 1107 70-71 9.55545176698556 1107 72-73 9.082993568510519 1107 74-75 8.965731209078635 1107 76-77 8.946118592171286 1107 78-79 8.605446357055412 1107 80-81 8.279901491174533 1107 82-83 8.11364594157146 1107 84-85 7.838243090273679 1107 86-87 7.473445626223011 1107 88-89 7.299860762884897 1107 90-91 7.1003748809755205 1107 92-93 6.569784351199491 1107 94-95 6.31833817370409 1107 96-97 5.8643979552565835 1107 98-99 5.421317205179083 1107 100-101 4.9432700770514355 1108 1 6.699949459871522 1108 2 7.938089741431821 1108 3 7.96876718177073 1108 4 9.425869241565614 1108 5 11.017939437994293 1108 6 9.462861400162865 1108 7 8.60167389560484 1108 8 5.682748816443109 1108 9 6.90932500908681 1108 10-11 5.99721110119253 1108 12-13 4.8932960755979416 1108 14-15 7.406016911826704 1108 16-17 8.458761758023318 1108 18-19 8.064319735041384 1108 20-21 8.228324652855115 1108 22-23 9.149837087748384 1108 24-25 9.025953438688934 1108 26-27 7.528307007080837 1108 28-29 9.493180904915526 1108 30-31 9.731727383578978 1108 32-33 10.152268020577974 1108 34-35 9.880837704691942 1108 36-37 9.752373953625895 1108 38-39 9.602078401837804 1108 40-41 9.715929106088275 1108 42-43 8.720250136765344 1108 44-45 8.359251508122533 1108 46-47 9.390640286670592 1108 48-49 9.874652836850807 1108 50-51 11.40754963313326 1108 52-53 10.547132280629626 1108 54-55 9.856151224364808 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-16.573981949484104 2313 60-61 -16.276092663792895 2313 62-63 -15.847391431193405 2313 64-65 -15.501415132520336 2313 66-67 -15.151361329009749 2313 68-69 -14.703787573421664 2313 70-71 -14.361260337703644 2313 72-73 -13.971532058534192 2313 74-75 -13.53685875820599 2313 76-77 -13.120947929093708 2313 78-79 -12.746107623315362 2313 80-81 -12.320970918858182 2313 82-83 -11.852820797796044 2313 84-85 -11.450606418995676 2313 86-87 -10.953353719469462 2313 88-89 -10.540379149874099 2313 90-91 -10.061293603212048 2313 92-93 -9.504779443784152 2313 94-95 -8.93084394189024 2313 96-97 -8.314173473314844 2313 98-99 -7.648475597438146 2313 100-101 -6.714854241378225 2314 1 -18.80311119677845 2314 2 -17.513217993682257 2314 3 -18.18009202802337 2314 4 -19.640908721706506 2314 5 -18.967313761783114 2314 6 -19.96479580629234 2314 7 -20.171559270783586 2314 8 -20.62136915795867 2314 9 -21.935137984791876 2314 10-11 -22.328748832029508 2314 12-13 -22.259875877657482 2314 14-15 -22.19623684443373 2314 16-17 -20.98670290530445 2314 18-19 -19.918568411870137 2314 20-21 -19.569393766733086 2314 22-23 -19.18836547207354 2314 24-25 -19.045693751071777 2314 26-27 -18.74534352157804 2314 28-29 -18.576379473492935 2314 30-31 -18.569469054929648 2314 32-33 -18.458889464118744 2314 34-35 -18.13947392580333 2314 36-37 -17.67639621888384 2314 38-39 -17.58935175954227 2314 40-41 -17.45351997571473 2314 42-43 -17.322877297847903 2314 44-45 -16.944031741738346 2314 46-47 -16.644279023290455 2314 48-49 -16.407484057973903 2314 50-51 -14.2261454141678 2314 52-53 -16.10784824246442 2314 54-55 -16.851416944806033 2314 56-57 -16.83070685221403 2314 58-59 -16.573981949484104 2314 60-61 -16.276092663792895 2314 62-63 -15.847391431193405 2314 64-65 -15.501415132520336 2314 66-67 -15.151361329009749 2314 68-69 -14.703787573421664 2314 70-71 -14.361260337703644 2314 72-73 -13.971532058534192 2314 74-75 -13.53685875820599 2314 76-77 -13.120947929093708 2314 78-79 -12.746107623315362 2314 80-81 -12.320970918858182 2314 82-83 -11.852820797796044 2314 84-85 -11.450606418995676 2314 86-87 -10.953353719469462 2314 88-89 -10.540379149874099 2314 90-91 -10.061293603212048 2314 92-93 -9.504779443784152 2314 94-95 -8.93084394189024 2314 96-97 -8.314173473314844 2314 98-99 -7.648475597438146 2314 100-101 -6.714854241378225 2315 1 -18.80311119677845 2315 2 -17.513217993682257 2315 3 -18.18009202802337 2315 4 -19.640908721706506 2315 5 -18.967313761783114 2315 6 -19.96479580629234 2315 7 -20.171559270783586 2315 8 -20.62136915795867 2315 9 -21.935137984791876 2315 10-11 -22.328748832029508 2315 12-13 -22.259875877657482 2315 14-15 -22.19623684443373 2315 16-17 -20.98670290530445 2315 18-19 -19.918568411870137 2315 20-21 -19.569393766733086 2315 22-23 -19.18836547207354 2315 24-25 -19.045693751071777 2315 26-27 -18.74534352157804 2315 28-29 -18.576379473492935 2315 30-31 -18.569469054929648 2315 32-33 -18.458889464118744 2315 34-35 -18.13947392580333 2315 36-37 -17.67639621888384 2315 38-39 -17.58935175954227 2315 40-41 -17.45351997571473 2315 42-43 -17.322877297847903 2315 44-45 -16.944031741738346 2315 46-47 -16.644279023290455 2315 48-49 -16.407484057973903 2315 50-51 -14.2261454141678 2315 52-53 -16.10784824246442 2315 54-55 -16.851416944806033 2315 56-57 -16.83070685221403 2315 58-59 -16.573981949484104 2315 60-61 -16.276092663792895 2315 62-63 -15.847391431193405 2315 64-65 -15.501415132520336 2315 66-67 -15.151361329009749 2315 68-69 -14.703787573421664 2315 70-71 -14.361260337703644 2315 72-73 -13.971532058534192 2315 74-75 -13.53685875820599 2315 76-77 -13.120947929093708 2315 78-79 -12.746107623315362 2315 80-81 -12.320970918858182 2315 82-83 -11.852820797796044 2315 84-85 -11.450606418995676 2315 86-87 -10.953353719469462 2315 88-89 -10.540379149874099 2315 90-91 -10.061293603212048 2315 92-93 -9.504779443784152 2315 94-95 -8.93084394189024 2315 96-97 -8.314173473314844 2315 98-99 -7.648475597438146 2315 100-101 -6.714854241378225 2316 1 -18.80311119677845 2316 2 -17.513217993682257 2316 3 -18.18009202802337 2316 4 -19.640908721706506 2316 5 -18.967313761783114 2316 6 -19.96479580629234 2316 7 -20.171559270783586 2316 8 -20.62136915795867 2316 9 -21.935137984791876 2316 10-11 -22.328748832029508 2316 12-13 -22.259875877657482 2316 14-15 -22.19623684443373 2316 16-17 -20.98670290530445 2316 18-19 -19.918568411870137 2316 20-21 -19.569393766733086 2316 22-23 -19.18836547207354 2316 24-25 -19.045693751071777 2316 26-27 -18.74534352157804 2316 28-29 -18.576379473492935 2316 30-31 -18.569469054929648 2316 32-33 -18.458889464118744 2316 34-35 -18.13947392580333 2316 36-37 -17.67639621888384 2316 38-39 -17.58935175954227 2316 40-41 -17.45351997571473 2316 42-43 -17.322877297847903 2316 44-45 -16.944031741738346 2316 46-47 -16.644279023290455 2316 48-49 -16.407484057973903 2316 50-51 -14.2261454141678 2316 52-53 -16.10784824246442 2316 54-55 -16.851416944806033 2316 56-57 -16.83070685221403 2316 58-59 -16.573981949484104 2316 60-61 -16.276092663792895 2316 62-63 -15.847391431193405 2316 64-65 -15.501415132520336 2316 66-67 -15.151361329009749 2316 68-69 -14.703787573421664 2316 70-71 -14.361260337703644 2316 72-73 -13.971532058534192 2316 74-75 -13.53685875820599 2316 76-77 -13.120947929093708 2316 78-79 -12.746107623315362 2316 80-81 -12.320970918858182 2316 82-83 -11.852820797796044 2316 84-85 -11.450606418995676 2316 86-87 -10.953353719469462 2316 88-89 -10.540379149874099 2316 90-91 -10.061293603212048 2316 92-93 -9.504779443784152 2316 94-95 -8.93084394189024 2316 96-97 -8.314173473314844 2316 98-99 -7.648475597438146 2316 100-101 -6.714854241378225 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 880451.0 3 35558.0 4 135070.0 5 72458.0 6 40366.0 7 40034.0 8 39061.0 9 37782.0 10 39963.0 11 42437.0 12 43820.0 13 45268.0 14 46191.0 15 47609.0 16 49528.0 17 50164.0 18 50004.0 19 52096.0 20 54585.0 21 58345.0 22 61861.0 23 66187.0 24 70758.0 25 75801.0 26 81923.0 27 87226.0 28 93373.0 29 100911.0 30 109042.0 31 118936.0 32 128195.0 33 136648.0 34 141732.0 35 134571.0 36 102147.0 37 44441.0 38 5562.0 39 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.610513528470676 21.34427026590351 14.128846820279003 22.916369385346805 2 14.5709141296119 21.265196656995293 42.25879392911144 21.905095284281366 3 18.61841175774284 26.741189902515206 30.301695005861305 24.338703333880645 4 11.93952784549003 17.376916613019684 40.65035132179777 30.03320421969251 5 12.206294398880283 38.51905274244483 36.42420656839467 12.850446290280217 6 28.938344946785243 39.41830355339069 18.60760968445382 13.035741815370239 7 25.700281032944982 33.16656752255496 23.766738787883945 17.36641265661611 8 24.013680883875843 37.32827770564368 21.153022308709723 17.505019101770756 9 25.081633453658597 17.08630905085776 21.97534008121124 35.8567174142724 10-11 23.479356856588513 27.661357572148543 29.776909759274083 19.082375811988857 12-13 24.06818150252087 25.122675314934927 29.985468548174282 20.823674634369922 14-15 21.373563450411673 27.03114516049185 27.55614077167541 24.039150617421072 16-17 20.773796493320123 30.027389793346483 28.22729005182322 20.971523661510176 18-19 20.42304086078869 29.01680502631741 30.341338624080667 20.218815488813235 20-21 21.28750842623801 28.379694062743066 30.23143375680581 20.101363754213118 22-23 20.863172478288313 28.272745037631115 29.918823769021134 20.94525871505944 24-25 20.78572757825765 28.32443839437276 30.293152105981953 20.59668192138764 26-27 20.723274355766907 28.60324015169194 30.03895784768302 20.63452764485813 28-29 20.672949430286973 28.670431160779174 30.101303832852032 20.555315576081814 30-31 21.06669139514045 28.415126683375973 29.97945291092555 20.53872901055803 32-33 20.790322783869627 28.614731142774456 29.87714281085745 20.717803262498467 34-35 20.931490909997184 28.585528153979123 30.01485421201316 20.46812672401053 36-37 20.986768523012643 28.38897311985923 29.92848574178897 20.695772615339163 38-39 20.873308477983983 28.64963310706507 30.00531104159855 20.471747373352397 40-41 21.144822411352003 28.480556643237126 29.91217971191949 20.462441233491383 42-43 21.00224407160815 28.492997738556973 29.747490619433247 20.757267570401627 44-45 21.011252555460075 28.814150191252335 29.533052685133192 20.64154456815439 46-47 21.064661165748156 28.616752428219105 29.313298622740515 21.00528778329223 48-49 21.053252619587546 28.670434460367254 29.382557382349216 20.89375553769598 50-51 21.244045001089265 28.876596391856395 28.958722968723666 20.92063563833067 52-53 20.74450993924446 29.139315359347716 29.075901056339355 21.04027364506847 54-55 20.779016438628865 29.051371672268715 29.15199797866883 21.017613910433592 56-57 20.821575303814615 29.090824216613388 29.186037419143133 20.901563060428867 58-59 20.712901211235376 29.017358920978115 29.33611225859452 20.933627609191984 60-61 20.762399600865102 29.18288216798167 29.217849114138396 20.83686911701483 62-63 20.684640980383364 29.065142835927794 29.153085896470053 21.09713028721879 64-65 20.536302178312756 29.035749938555742 29.185321194172957 21.242626688958545 66-67 20.622015268539457 28.99033470987141 28.982451143842436 21.4051988777467 68-69 20.607874625133018 29.001470445970785 29.057753700299894 21.332901228596306 70-71 20.722460568565058 28.692557488493197 29.156109838792926 21.42887210414882 72-73 20.731351123688558 28.810986546024143 29.05733201459447 21.400330315692834 74-75 20.57424966162787 29.13396832310665 28.786808018030925 21.504973997234558 76-77 20.578106852551727 29.037968048355506 28.921504528060275 21.462420571032492 78-79 20.51271554803982 29.069101903015294 28.823484316200638 21.594698232744246 80-81 20.59159371944296 29.042730549742952 28.656974141744314 21.708701589069776 82-83 20.778423318516015 28.815408688838023 28.91151174610938 21.494656246536582 84-85 20.885839474938585 28.89412605873941 28.60261064056026 21.61742382576174 86-87 20.58900042547024 28.92017523763214 28.846077959239015 21.644746377658606 88-89 20.80344158366508 28.843016994901603 28.67284316883381 21.680698252599502 90-91 20.75550207760828 28.83932271596275 28.75184053718544 21.653334669243527 92-93 20.812305861630996 28.76660903206951 28.584081826303535 21.837003279995947 94-95 20.570168986877903 28.705971986926077 28.742534531856386 21.981324494339635 96-97 20.572480606676432 28.735000181156682 28.632824170780317 22.059695041386565 98-99 20.48156006457968 28.74959935977104 28.423862809939166 22.344977765710112 100-101 20.832132257710906 28.77873769504945 28.32865267550437 22.060477371735274 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 208860.0 1 189811.0 2 126313.5 3 64053.5 4 39760.0 5 31315.5 6 29204.0 7 29415.0 8 30020.0 9 29493.0 10 26887.5 11 22599.5 12 17751.5 13 13256.5 14 9877.5 15 8067.5 16 7483.5 17 8010.0 18 9372.0 19 10963.5 20 12794.5 21 14843.5 22 17065.0 23 19405.5 24 21719.5 25 24369.0 26 28188.0 27 32990.0 28 38380.0 29 45218.5 30 53028.0 31 60981.0 32 69220.5 33 77593.5 34 85195.0 35 91663.5 36 98327.0 37 104115.0 38 106390.5 39 106035.5 40 104601.0 41 102518.0 42 100858.5 43 100419.0 44 100220.0 45 99468.5 46 97570.5 47 94614.5 48 90694.0 49 85323.0 50 79313.5 51 73033.0 52 66269.5 53 59324.5 54 52041.5 55 44099.0 56 36469.5 57 29424.0 58 23275.0 59 18473.0 60 14078.0 61 10244.0 62 7418.5 63 5151.0 64 3432.0 65 2212.5 66 1432.5 67 919.5 68 546.0 69 339.5 70 224.5 71 165.5 72 118.0 73 71.0 74 50.0 75 32.0 76 21.0 77 16.5 78 14.5 79 10.5 80 4.5 81 3.5 82 5.5 83 4.0 84 1.5 85 1.0 86 0.5 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.5468321609151 2 29.670080660464382 3 23.42796593939603 4 29.7834971034803 5 32.98500441063945 6 25.465046888445176 7 24.404768344210574 8 20.351395074878535 9 12.051586207461158 10-11 15.744389336222882 12-13 15.794065679786698 14-15 19.971951432383303 16-17 23.401607361572147 18-19 27.43520073306962 20-21 29.516753558846588 22-23 29.153127986903453 24-25 27.648130675390682 26-27 29.08352555261643 28-29 26.01736362177093 30-31 27.953337569028626 32-33 26.903073237666657 34-35 24.916545789513233 36-37 27.95251888885218 38-39 28.101518680965892 40-41 25.067650759223532 42-43 25.941723421182587 44-45 24.59978965767181 46-47 27.978804370231735 48-49 23.30919883665547 50-51 28.867510940344822 52-53 22.470694904505343 54-55 23.624741567521085 56-57 25.777475710782515 58-59 25.04453766352771 60-61 24.576778896998047 62-63 24.395938293736013 64-65 22.911437225212193 66-67 23.41325893479768 68-69 24.440205500419722 70-71 22.90023884994148 72-73 22.768855300910285 74-75 19.98477254871805 76-77 22.298932879628577 78-79 21.432900826545353 80-81 22.465051859003392 82-83 23.361462794348416 84-85 20.2576269560974 86-87 21.830296955767004 88-89 20.66398470939339 90-91 21.79176051031844 92-93 21.764422440140592 94-95 20.791040948920497 96-97 19.703804436018537 98-99 19.129441815084125 100-101 20.78166998475793 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3420139.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.43861102080848 #Duplication Level Percentage of deduplicated Percentage of total 1 87.0140209654857 63.90188839040778 2 8.40965204711035 12.351863310161654 3 2.2284385742181794 4.909603009073217 4 0.9013601014571314 2.6477853552234696 5 0.4342275015710834 1.5944532291208149 6 0.2427642262664947 1.0696960549531584 7 0.1614464277641448 0.8299480986489061 8 0.11466063717981384 0.6736414346597118 9 0.08016784990037001 0.529867399068705 >10 0.3876350633146007 4.950571975621256 >50 0.01829006346327125 0.9060204853639082 >100 0.007014400648041998 0.8618224926673791 >500 2.0133851332815019E-4 0.09897762291686348 >1k 8.053540496375208E-5 0.1013729578941118 >5k 0.0 0.0 >10k+ 4.026770248187604E-5 4.572488184219182 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 154622 4.520927365817588 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.9238577730320317E-5 0.0 5 0.0 0.0 0.0 2.9238577730320317E-5 0.0 6 0.0 0.0 0.0 2.9238577730320317E-5 0.0 7 0.0 0.0 0.0 2.9238577730320317E-5 0.0 8 0.0 0.0 0.0 2.9238577730320317E-5 0.0 9 0.0 0.0 0.0 2.9238577730320317E-5 0.0 10-11 0.0 0.0 0.0 5.8477155460640633E-5 0.0 12-13 0.0 0.0 0.0 5.8477155460640633E-5 0.0 14-15 0.0 0.0 0.0 5.8477155460640633E-5 0.0 16-17 0.0 0.0 0.0 8.771573319096096E-5 0.0 18-19 0.0 0.0 0.0 8.771573319096096E-5 0.0 20-21 0.0 0.0 0.0 1.1695431092128127E-4 0.0 22-23 0.0 0.0 0.0 1.1695431092128127E-4 0.0 24-25 0.0 0.0 0.0 2.485279107077227E-4 0.0 26-27 0.0 0.0 0.0 3.508629327638438E-4 0.0 28-29 0.0 0.0 0.0 5.701522657412462E-4 0.0 30-31 0.0 0.0 0.0 0.0021928933297740236 0.0 32-33 0.0 0.0 0.0 0.005789238390603423 0.0 34-35 0.0 0.0 0.0 0.011549238203476525 0.0 36-37 0.0 0.0 0.0 0.021461116054055113 0.0 38-39 0.0 0.0 0.0 0.037542333805731284 0.0 40-41 0.0 0.0 0.0 0.05347735866875586 0.0 42-43 0.0 0.0 0.0 0.06992405864206104 0.0 44-45 0.0 0.0 0.0 0.09185299193980129 0.0 46-47 0.0 0.0 0.0 0.11898639207353853 0.0 48-49 0.0 0.0 0.0 0.1500085230454084 0.0 50-51 1.4619288865160158E-5 0.0 0.0 0.1879748162282293 0.0 52-53 2.9238577730320317E-5 0.0 0.0 0.23064852042563183 0.0 54-55 2.9238577730320317E-5 1.4619288865160158E-5 0.0 0.2769331889727289 0.0 56-57 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.3335829333252245 0.0 58-59 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.39796628148738983 0.0 60-61 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.46809501017356314 0.0 62-63 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.5387500332588822 0.0 64-65 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.6162030256665008 0.0 66-67 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.7023837335266199 2.9238577730320317E-5 68-69 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.8049672834934487 2.9238577730320317E-5 70-71 2.9238577730320317E-5 2.9238577730320317E-5 0.0 0.9164978382457556 2.9238577730320317E-5 72-73 2.9238577730320317E-5 2.9238577730320317E-5 1.4619288865160158E-5 1.0328966161901607 2.9238577730320317E-5 74-75 2.9238577730320317E-5 2.9238577730320317E-5 2.9238577730320317E-5 1.152043820441216 2.9238577730320317E-5 76-77 5.8477155460640633E-5 2.9238577730320317E-5 2.9238577730320317E-5 1.2831203644062419 2.9238577730320317E-5 78-79 5.8477155460640633E-5 2.9238577730320317E-5 2.9238577730320317E-5 1.429517923101956 2.9238577730320317E-5 80-81 5.8477155460640633E-5 2.9238577730320317E-5 2.9238577730320317E-5 1.593941064968412 2.9238577730320317E-5 82-83 5.8477155460640633E-5 2.9238577730320317E-5 2.9238577730320317E-5 1.7657615670006395 2.9238577730320317E-5 84-85 5.8477155460640633E-5 2.9238577730320317E-5 2.9238577730320317E-5 1.930930292599219 2.9238577730320317E-5 86-87 5.8477155460640633E-5 5.8477155460640633E-5 2.9238577730320317E-5 2.1075897792458145 2.9238577730320317E-5 88-89 8.771573319096096E-5 5.8477155460640633E-5 2.9238577730320317E-5 2.306222057056745 2.9238577730320317E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2360 0.0 52.602787 1 GTATCAA 4690 0.0 46.157787 1 ATCAACG 5570 0.0 36.121117 3 TATCAAC 5950 0.0 34.99744 2 CAACGCA 5775 0.0 34.915394 5 TCAACGC 5765 0.0 34.89701 4 AACGCAG 6410 0.0 32.692696 6 ACGCAGA 7485 0.0 26.936396 7 CGCAGAG 7585 0.0 26.61719 8 GTACATG 5820 0.0 26.530712 1 TACATGG 5980 0.0 25.215834 2 ACATGGG 5810 0.0 24.908592 3 GCAGAGT 8145 0.0 23.353865 9 GTGTAGC 1330 0.0 21.98511 1 GAGTACT 5075 0.0 20.586742 12-13 CATGGGG 3545 0.0 20.196993 4 GGCGTCG 290 2.728484E-10 19.847157 8 AGAGTAC 7315 0.0 19.51357 10-11 CAGAGTA 8040 0.0 18.892025 10-11 GAGTACA 4200 0.0 18.443064 1 >>END_MODULE