FastQCFastQC Report
Sat 18 Jun 2016
SRR1931024_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931024_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3420139
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.1998749173644697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53210.1555784721030344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.053.464951
GTATCAA81450.042.4773061
ATCAACG99200.034.4899833
CAACGCA99750.034.248165
TCAACGC101600.033.534464
TATCAAC105400.032.599282
AACGCAG107250.032.0301486
CGCAGAG123150.027.3549068
ACGCAGA123550.027.3047497
GCAGAGT140750.023.1246059
ACGGGCG4800.021.7469885
GTACATG94700.021.7297441
TACGGGC5600.021.2137664
TACATGG95350.021.1327252
ACATGGG90800.021.090723
GCGTCGG5000.020.8761949
GGCGTCG5050.020.6701038
TAACGCC2351.1287739E-720.2207814
AGAGTAC131050.020.07535210-11
GAGTACT98400.019.55214312-13
Produced by FastQC (version 0.11.5)