##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931024_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3420139 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3310441476209 31.0 31.0 34.0 30.0 34.0 2 31.494422302719276 31.0 31.0 34.0 30.0 34.0 3 31.567686576481247 31.0 31.0 34.0 30.0 34.0 4 35.337664054004826 37.0 35.0 37.0 33.0 37.0 5 35.211514502773134 37.0 35.0 37.0 33.0 37.0 6 35.237519878578034 37.0 35.0 37.0 32.0 37.0 7 35.119805364635766 37.0 35.0 37.0 32.0 37.0 8 35.121219049869026 37.0 35.0 37.0 32.0 37.0 9 36.62788822325642 39.0 35.0 39.0 32.0 39.0 10-11 36.56762371353913 38.0 35.0 39.0 32.0 39.0 12-13 36.45571729687009 38.0 35.0 39.0 32.0 39.0 14-15 37.52226035257631 39.0 36.0 41.0 32.0 41.0 16-17 37.48140587268529 39.0 36.0 41.0 32.0 41.0 18-19 37.508732978396495 39.0 36.0 41.0 32.0 41.0 20-21 37.48996298103673 39.0 36.0 41.0 32.0 41.0 22-23 37.39015987361917 39.0 36.0 41.0 32.0 41.0 24-25 37.268973132378534 39.0 36.0 40.5 31.0 41.0 26-27 37.01519689696822 39.0 36.0 40.0 31.0 41.0 28-29 36.836528427645774 39.0 36.0 40.0 30.5 41.0 30-31 36.625336426385005 38.5 35.0 40.0 30.0 41.0 32-33 36.475270449534364 38.0 35.0 40.0 30.0 41.0 34-35 36.56128011171476 38.0 35.0 40.0 30.0 41.0 36-37 36.63413796924628 39.0 35.0 40.0 30.0 41.0 38-39 36.623770261968886 39.0 35.0 40.0 30.0 41.0 40-41 36.51817148367362 39.0 35.0 40.0 30.0 41.0 42-43 36.38902249294546 39.0 35.0 40.0 30.0 41.0 44-45 36.238577876513205 39.0 35.0 40.0 30.0 41.0 46-47 36.020103861275814 38.0 35.0 40.0 29.5 41.0 48-49 35.82196396111386 38.0 35.0 40.0 28.5 41.0 50-51 35.576459319343456 38.0 34.5 40.0 27.5 41.0 52-53 35.37723832276992 38.0 34.0 40.0 27.0 41.0 54-55 35.239487781052176 38.0 34.0 40.0 27.0 41.0 56-57 35.076024395499715 38.0 34.0 40.0 27.0 41.0 58-59 34.87911953286109 38.0 34.0 40.0 26.0 41.0 60-61 34.58816629967379 38.0 33.0 40.0 26.0 41.0 62-63 34.34978505259581 37.0 33.0 40.0 26.0 41.0 64-65 34.04914434764201 37.0 33.0 40.0 25.5 41.0 66-67 33.741940459145084 36.5 32.0 39.0 25.0 41.0 68-69 33.40387510566091 36.0 32.0 39.0 23.5 40.5 70-71 33.04570355181471 36.0 31.5 39.0 23.0 40.0 72-73 32.63112727289739 35.0 31.0 38.5 22.0 40.0 74-75 32.123481531013795 35.0 31.0 37.5 22.0 39.5 76-77 30.717404760449796 33.5 29.5 36.0 20.5 39.0 78-79 31.464057162589008 34.0 30.5 36.0 21.5 39.0 80-81 31.431024587012402 34.0 31.0 36.0 21.5 39.0 82-83 31.15072940017935 34.0 31.0 36.0 20.5 37.5 84-85 30.804104453064628 34.0 30.5 35.5 20.0 37.0 86-87 30.451343205641642 34.0 30.0 35.0 19.5 36.5 88-89 30.096160857789698 34.0 30.0 35.0 18.0 36.0 90-91 29.75414712676882 34.0 29.5 35.0 15.0 36.0 92-93 29.43357565291937 34.0 29.0 35.0 8.5 35.5 94-95 29.05186806735048 34.0 29.0 35.0 2.0 35.0 96-97 28.6343258271082 34.0 29.0 35.0 2.0 35.0 98-99 28.14370366233653 34.0 29.0 35.0 2.0 35.0 100-101 27.007971459639506 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3301344242776345 1101 2 0.4002528655465909 1101 3 0.3195650245077317 1101 4 0.24713041715236272 1101 5 0.3278520097950306 1101 6 0.42199816588887273 1101 7 0.3784830204812266 1101 8 0.3796635829617543 1101 9 0.4776081096454803 1101 10-11 0.5369647489381038 1101 12-13 0.43253886874889247 1101 14-15 0.5638674875048295 1101 16-17 0.5020079644613915 1101 18-19 0.48365265960354265 1101 20-21 0.4235060612268384 1101 22-23 0.5366209230011876 1101 24-25 0.548643280095682 1101 26-27 0.5097403207158209 1101 28-29 0.6107958264467825 1101 30-31 0.4515467543465306 1101 32-33 0.37482006639130105 1101 34-35 0.49431976520590837 1101 36-37 0.45835671047621673 1101 38-39 0.47524547447658705 1101 40-41 0.5061152653087575 1101 42-43 0.4835767060171392 1101 44-45 0.6050952941587937 1101 46-47 0.46506457711657134 1101 48-49 0.46085653823796235 1101 50-51 0.46545407762119595 1101 52-53 0.3767354089926087 1101 54-55 0.22609514016277643 1101 56-57 0.3577605538829616 1101 58-59 0.42327082886858136 1101 60-61 0.3523550002673588 1101 62-63 0.17879121522571495 1101 64-65 0.1939561587029246 1101 66-67 0.2569358002590221 1101 68-69 0.3005842656530646 1101 70-71 0.3285994845889135 1101 72-73 0.3072134016038035 1101 74-75 0.16731891428996448 1101 76-77 0.3203566490765013 1101 78-79 0.36523922141675413 1101 80-81 0.2051353143725727 1101 82-83 0.2819023886783363 1101 84-85 0.25927135571435045 1101 86-87 0.29700553259381834 1101 88-89 0.368784353498782 1101 90-91 0.2897626952164565 1101 92-93 0.10852968676952912 1101 94-95 0.33450802662932944 1101 96-97 0.4094166519651452 1101 98-99 0.45677532260894793 1101 100-101 0.6255493465971469 1102 1 0.3233616583774719 1102 2 0.4707179857391033 1102 3 0.38573064364429754 1102 4 0.2198441511554421 1102 5 0.2708360822891649 1102 6 0.3429794786249829 1102 7 0.4298793065873596 1102 8 0.38156007520834834 1102 9 0.45988928523508577 1102 10-11 0.5041749533116473 1102 12-13 0.48792207966003076 1102 14-15 0.571099862495366 1102 16-17 0.6084936061471993 1102 18-19 0.595002164445738 1102 20-21 0.5480798973746204 1102 22-23 0.5525246596125726 1102 24-25 0.6417058121736616 1102 26-27 0.6224413076658735 1102 28-29 0.5995817853364898 1102 30-31 0.5074518371813426 1102 32-33 0.6031010652890814 1102 34-35 0.7852049912452372 1102 36-37 0.6388718092132919 1102 38-39 0.5581551681266177 1102 40-41 0.6415939318744606 1102 42-43 0.6311497176030727 1102 44-45 0.6832801642903092 1102 46-47 0.6564872407878894 1102 48-49 0.5803454361016733 1102 50-51 0.3966715263595262 1102 52-53 0.33388869335707483 1102 54-55 0.4303407922818181 1102 56-57 0.48990710713029983 1102 58-59 0.48377010948043164 1102 60-61 0.42305634060105035 1102 62-63 0.17143115935744646 1102 64-65 0.2202291812269337 1102 66-67 0.21395109672265988 1102 68-69 0.2824245530717633 1102 70-71 0.3917710186946408 1102 72-73 0.31843291174676125 1102 74-75 0.35373888551443855 1102 76-77 0.32328868162144886 1102 78-79 0.5021865335975519 1102 80-81 0.47567426650591926 1102 82-83 0.4822553516319452 1102 84-85 0.5135857914363875 1102 86-87 0.496081476422642 1102 88-89 0.40469991163459795 1102 90-91 0.3651570966097957 1102 92-93 0.4429625060137923 1102 94-95 0.4822217606324095 1102 96-97 0.5089204424254703 1102 98-99 0.5812477713052182 1102 100-101 0.9451734978453494 1103 1 0.3236678222122187 1103 2 0.37229463880947833 1103 3 0.3821234500307078 1103 4 0.29735124881461417 1103 5 0.3202361198795529 1103 6 0.3393999831935375 1103 7 0.3284089947519959 1103 8 0.32366691764855915 1103 9 0.44151220086444454 1103 10-11 0.4351491049723819 1103 12-13 0.42936647391068306 1103 14-15 0.4500306961776701 1103 16-17 0.47783526322391623 1103 18-19 0.4194098684972545 1103 20-21 0.44451927957326376 1103 22-23 0.44456629591565644 1103 24-25 0.518463492648749 1103 26-27 0.5145449351385167 1103 28-29 0.46228651282264366 1103 30-31 0.4612574519985557 1103 32-33 0.5951168169814807 1103 34-35 0.4780774905775047 1103 36-37 0.36567375731876695 1103 38-39 0.4360109661145444 1103 40-41 0.5151875896502318 1103 42-43 0.4884915494421378 1103 44-45 0.5535652906962341 1103 46-47 0.5869515315660436 1103 48-49 0.44854784056767727 1103 50-51 0.3901874313811433 1103 52-53 0.5859969701674217 1103 54-55 0.6192438783209511 1103 56-57 0.4944761530927977 1103 58-59 0.5724807920757513 1103 60-61 0.5307975703343928 1103 62-63 0.4714829285788298 1103 64-65 0.3611521736857668 1103 66-67 0.38094467338232363 1103 68-69 0.3615584243213803 1103 70-71 0.3985221208514744 1103 72-73 0.33535584155495 1103 74-75 0.3877003190932058 1103 76-77 0.45386244616711835 1103 78-79 0.5746856185683242 1103 80-81 0.5262260044019804 1103 82-83 0.5291609041282435 1103 84-85 0.48960089683786023 1103 86-87 0.4407305338601013 1103 88-89 0.48577826121863055 1103 90-91 0.4959165873589306 1103 92-93 0.32060916623953517 1103 94-95 0.3953392404935272 1103 96-97 0.4511919076922766 1103 98-99 0.45175423586308483 1103 100-101 0.7726385536209115 1104 1 0.3093009525204131 1104 2 0.38188444637199126 1104 3 0.35770947998507197 1104 4 0.14013921106811011 1104 5 0.25894964354634453 1104 6 0.25806318697236463 1104 7 0.2884604912245692 1104 8 0.29605752380984285 1104 9 0.42167107836414175 1104 10-11 0.3841937488039875 1104 12-13 0.4471216099355999 1104 14-15 0.6148648162314103 1104 16-17 0.5213113908524889 1104 18-19 0.48428042731226384 1104 20-21 0.485922560862015 1104 22-23 0.45920271921415434 1104 24-25 0.4138964235483513 1104 26-27 0.46965891516583014 1104 28-29 0.5876200994752878 1104 30-31 0.5212418895774462 1104 32-33 0.5549683583635812 1104 34-35 0.4441001941600007 1104 36-37 0.4520887538219611 1104 38-39 0.520688129459046 1104 40-41 0.4684675362602917 1104 42-43 0.39747581776643415 1104 44-45 0.5604657103286783 1104 46-47 0.3790544949358434 1104 48-49 0.32139416431136425 1104 50-51 0.31436262065133747 1104 52-53 0.2029629827848467 1104 54-55 0.24593909645282253 1104 56-57 0.36062205247841916 1104 58-59 0.4457541903173379 1104 60-61 0.3776910011827965 1104 62-63 0.13254607082806302 1104 64-65 0.3188378831815655 1104 66-67 0.3074414473383982 1104 68-69 0.27577821289941795 1104 70-71 0.32431523452031996 1104 72-73 0.2964489449206411 1104 74-75 0.3071159664148624 1104 76-77 0.3426447405617701 1104 78-79 0.3889867313248274 1104 80-81 0.4148663290750356 1104 82-83 0.369490134042362 1104 84-85 0.3832024464576662 1104 86-87 0.39202965526321876 1104 88-89 0.46613608921128247 1104 90-91 0.25801366941301396 1104 92-93 0.3028287143571049 1104 94-95 0.5007768938051527 1104 96-97 0.5493803049284622 1104 98-99 0.4660445552238315 1104 100-101 0.6701709326026766 1105 1 0.35276143844858154 1105 2 0.3373620503840904 1105 3 0.39029769470563025 1105 4 0.26028413717745025 1105 5 0.37033627304381156 1105 6 0.345956052671454 1105 7 0.2777523120791727 1105 8 0.3691042773890416 1105 9 0.45603828545629455 1105 10-11 0.44537395048719475 1105 12-13 0.4684182982296008 1105 14-15 0.5187993453739281 1105 16-17 0.5401421270773099 1105 18-19 0.5723498091868464 1105 20-21 0.540470630225137 1105 22-23 0.5105155963621826 1105 24-25 0.5804444750957671 1105 26-27 0.5824321225889832 1105 28-29 0.5708967741637139 1105 30-31 0.43900433959382923 1105 32-33 0.6481892933226447 1105 34-35 0.7340330523764891 1105 36-37 0.6753235803461806 1105 38-39 0.7525372032062378 1105 40-41 0.6447265560493278 1105 42-43 0.6247696958666609 1105 44-45 0.6592867557451356 1105 46-47 0.5398358825827145 1105 48-49 0.5695842288651818 1105 50-51 0.4304193102393157 1105 52-53 0.3977205695813453 1105 54-55 0.45478047053162385 1105 56-57 0.5782911106696886 1105 58-59 0.7438310547899505 1105 60-61 0.707492604502896 1105 62-63 0.5402204924855951 1105 64-65 0.5337531249430825 1105 66-67 0.6004928751971974 1105 68-69 0.5652667332531536 1105 70-71 0.5365703095361738 1105 72-73 0.6272204495370204 1105 74-75 0.6133005024726899 1105 76-77 0.6634378068751232 1105 78-79 0.6714427347266962 1105 80-81 0.6316475320011286 1105 82-83 0.6282067043869901 1105 84-85 0.7119189324120896 1105 86-87 0.6818116659910878 1105 88-89 0.6802796294459519 1105 90-91 0.7369989850487819 1105 92-93 0.8008760188168011 1105 94-95 0.8330194837592515 1105 96-97 0.8125216177305958 1105 98-99 0.9983989387557415 1105 100-101 1.1557193446837921 1106 1 0.2671389086981577 1106 2 0.2478219584080108 1106 3 0.2971523466553876 1106 4 0.11703107036174032 1106 5 0.16596773152735267 1106 6 0.2600011125406212 1106 7 0.3604617237128025 1106 8 0.22497153545766224 1106 9 0.3739780409090727 1106 10-11 0.36068879959344713 1106 12-13 0.37260016200517754 1106 14-15 0.4166212133115508 1106 16-17 0.4081099824103447 1106 18-19 0.39896203789868423 1106 20-21 0.38091679370904075 1106 22-23 0.4086499239186949 1106 24-25 0.48397087962170104 1106 26-27 0.5410378221839167 1106 28-29 0.670056432741724 1106 30-31 0.469745470482799 1106 32-33 0.5125240382490901 1106 34-35 0.6367033390320955 1106 36-37 0.511419480280523 1106 38-39 0.45137485649330955 1106 40-41 0.5360694141831459 1106 42-43 0.5964142156533612 1106 44-45 0.5757263673989641 1106 46-47 0.551364660328467 1106 48-49 0.6195367153592812 1106 50-51 0.5537243012139044 1106 52-53 0.5562685040483117 1106 54-55 0.7146175771678216 1106 56-57 0.7354434126345879 1106 58-59 0.7880038056715506 1106 60-61 0.8078466230927077 1106 62-63 0.6943321449273654 1106 64-65 0.6723339536117763 1106 66-67 0.6752961069750185 1106 68-69 0.6417294079872988 1106 70-71 0.6107207677437785 1106 72-73 0.8090196153496407 1106 74-75 0.7706597552215229 1106 76-77 0.7712080606580187 1106 78-79 0.7454434087554915 1106 80-81 0.6421590511104291 1106 82-83 0.8056500818237815 1106 84-85 0.8993499680008732 1106 86-87 0.8105992796989199 1106 88-89 0.8898130394515995 1106 90-91 0.8409167467820531 1106 92-93 0.7624657384656288 1106 94-95 0.8207064108846396 1106 96-97 0.8485055018123759 1106 98-99 0.8655607833233496 1106 100-101 0.9994566164816092 1107 1 0.1678763172613671 1107 2 0.20891775755092823 1107 3 0.17352793438250558 1107 4 0.1557166557412657 1107 5 0.12590980219763281 1107 6 0.1807518703774349 1107 7 0.24478245268452525 1107 8 0.20492982199511545 1107 9 0.16422139358034116 1107 10-11 0.17086357731174928 1107 12-13 0.2766319655029079 1107 14-15 0.2799481425742769 1107 16-17 0.30410823288303135 1107 18-19 0.3082191134059684 1107 20-21 0.3503156056719732 1107 22-23 0.2894537952080398 1107 24-25 0.2786231286303149 1107 26-27 0.4365372853765592 1107 28-29 0.43258933067470196 1107 30-31 0.4494699787714751 1107 32-33 0.44094957229547305 1107 34-35 0.3368317775110796 1107 36-37 0.3483857431900006 1107 38-39 0.3410103253684369 1107 40-41 0.32678801789257506 1107 42-43 0.27162916490862443 1107 44-45 0.24335133126479747 1107 46-47 0.16794487153907767 1107 48-49 0.18503385208492773 1107 50-51 0.20071997537334596 1107 52-53 0.2954686127978192 1107 54-55 0.41341531273996424 1107 56-57 0.3861837632280327 1107 58-59 0.3009023451572119 1107 60-61 0.3236364239338556 1107 62-63 0.21604825077769618 1107 64-65 0.26720899728960745 1107 66-67 0.32660419396737694 1107 68-69 0.35932475541542175 1107 70-71 0.2635245376462336 1107 72-73 0.34155303832185524 1107 74-75 0.41060738104837924 1107 76-77 0.3563516981178161 1107 78-79 0.4033873581006766 1107 80-81 0.42650491558150705 1107 82-83 0.5223718725817648 1107 84-85 0.46969896520609566 1107 86-87 0.536575264283286 1107 88-89 0.5719348950247678 1107 90-91 0.5945386877495373 1107 92-93 0.6135629497830593 1107 94-95 0.7206297068758154 1107 96-97 0.814083672611865 1107 98-99 0.8080097685190495 1107 100-101 1.1034948019218476 1108 1 0.09070237717341101 1108 2 0.23382750352886816 1108 3 0.09715382486154667 1108 4 0.08195216032937225 1108 5 0.13407635750701274 1108 6 0.13976844289105372 1108 7 0.22923597360786374 1108 8 0.2836406140640477 1108 9 0.35276855415148844 1108 10-11 0.16854540374123417 1108 12-13 0.13395255434327424 1108 14-15 0.11866235425265614 1108 16-17 0.2001995161256147 1108 18-19 0.24609357884546057 1108 20-21 0.18980044317832068 1108 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-2.5029401330610703 2316 76-77 -1.5418755244092566 2316 78-79 -1.3829462909324306 2316 80-81 -1.2183359059515624 2316 82-83 -1.055204825915009 2316 84-85 -0.9888861243944866 2316 86-87 -0.8716406792633826 2316 88-89 -0.8788838167649011 2316 90-91 -0.9911336056731059 2316 92-93 -0.9430157131889132 2316 94-95 -1.4366283098622645 2316 96-97 -1.072799400255537 2316 98-99 -1.0648627309937098 2316 100-101 -1.5829566931508232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 11.0 4 24.0 5 67.0 6 154.0 7 386.0 8 1042.0 9 2055.0 10 3617.0 11 5711.0 12 8052.0 13 10921.0 14 13538.0 15 16677.0 16 19467.0 17 22294.0 18 19407.0 19 15885.0 20 15432.0 21 16453.0 22 18406.0 23 21660.0 24 26792.0 25 34485.0 26 44779.0 27 58371.0 28 73876.0 29 92893.0 30 114879.0 31 139656.0 32 168671.0 33 205600.0 34 254012.0 35 316338.0 36 416013.0 37 572808.0 38 574056.0 39 115629.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.700312985384656 22.486116829163315 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29.407562090312705 30.591285909724718 19.95955427542565 20-21 20.77861455338511 28.66851318031226 30.589867838704798 19.963004427597824 22-23 20.42984076322312 28.457568973315905 30.425142107296015 20.68744815616496 24-25 20.36686442781669 28.612059989725502 30.599388500809475 20.42168708164833 26-27 20.40066724268248 28.77561552552671 30.474701356268703 20.349015875522106 28-29 20.306020218216627 28.8808463178411 30.453241079624522 20.359892384317746 30-31 20.69023493953445 28.590223901272616 30.39413904847259 20.325402110720344 32-33 20.534640064899857 28.853312050984055 30.189458920250267 20.422588963865824 34-35 20.57403073827868 28.82156285205257 30.42601239444363 20.17839401522512 36-37 20.630137995642244 28.620221934512006 30.45684522243157 20.292794847414175 38-39 20.48794329807835 28.819627084459633 30.482575082650342 20.209854534811676 40-41 20.645234388134764 28.738901924665395 30.3790267757286 20.23683691147124 42-43 20.587785914665947 28.7186177580018 30.17074208819742 20.522854239134837 44-45 20.518093596157065 29.066453715973996 30.021603071917056 20.393849615951886 46-47 20.638214502416094 28.86852205188938 29.7976182643595 20.695645181335028 48-49 20.68541511746268 28.907188706131148 29.825131361288648 20.582264815117522 50-51 20.74059785420222 29.066095211647514 29.49087833819896 20.702428595951304 52-53 20.71927639727781 29.19143826739761 29.363847291268364 20.72543804405621 54-55 20.74607964283067 29.108601719902406 29.466119051472933 20.679199585794 56-57 20.719571778079477 29.19484114928204 29.346073243469345 20.73951382916914 58-59 20.579361083869678 29.115837855281384 29.615731773311822 20.689069287537116 60-61 20.64768551486307 29.240492896156468 29.49848979744184 20.613331791538624 62-63 20.645627481958154 29.115575519743047 29.48225211705105 20.75654488124775 64-65 20.403952177595926 29.112533303632404 29.516763927439847 20.966750591331824 66-67 20.5757716060918 29.111244681314147 29.35347380482638 20.959509907767675 68-69 20.511006565834215 29.12180501817329 29.4098077148552 20.957380701137296 70-71 20.564716198516994 28.812064402170968 29.5117904669852 21.11142893232684 72-73 20.595016940679727 29.000915011950507 29.422078217781046 20.981989829588723 74-75 20.48527524098345 29.214166972899598 29.257613693280522 21.042944092836436 76-77 20.374325173991284 29.088992945420323 29.40268119733896 21.134000683249432 78-79 20.367494536457052 29.11018666398436 29.31679835505982 21.205520444498767 80-81 20.434599422596396 29.13306422138522 29.207388707707118 21.224947648311268 82-83 20.527689079303503 28.980342611806126 29.412181785594093 21.079786523296278 84-85 20.647625725153276 28.967550734049112 29.10988413044031 21.274939410357298 86-87 20.267494976081384 28.97541591145857 29.348295493253346 21.408793619206705 88-89 20.424336678812374 28.887065375724607 29.24599826088889 21.44259968457413 90-91 20.434113133045102 28.835975973833445 29.37382198156809 21.356088911553368 92-93 20.4702453609176 28.834996230480524 29.223499459283047 21.471258949318823 94-95 20.132854821670684 28.808607712930428 29.31797176005373 21.74056570534516 96-97 20.139418330573456 28.747651593641603 29.357568414261586 21.755361661523356 98-99 20.034141887215696 28.806753175821214 29.23351945637297 21.925585480590115 100-101 20.158595893324804 28.704505869498288 29.128816109520695 22.008082127656216 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 127.5 2 96.0 3 145.0 4 133.0 5 128.0 6 257.5 7 551.5 8 942.0 9 1156.5 10 1159.5 11 1180.0 12 1367.5 13 1603.0 14 1789.5 15 2096.0 16 2414.0 17 2805.0 18 3330.0 19 3845.5 20 4451.5 21 5307.0 22 6505.0 23 8284.5 24 10619.5 25 13992.5 26 19704.0 27 26836.0 28 35087.5 29 46655.0 30 59669.5 31 72043.0 32 85972.5 33 100062.0 34 112468.5 35 122967.0 36 135461.5 37 146564.5 38 148882.5 39 146801.0 40 144171.0 41 141173.5 42 138678.0 43 138932.0 44 139837.5 45 140877.5 46 139719.0 47 135099.5 48 130114.0 49 123185.0 50 114125.0 51 104834.5 52 94782.5 53 84323.0 54 73590.0 55 61153.5 56 48787.5 57 37739.0 58 28752.0 59 22204.5 60 16224.0 61 10936.5 62 7264.5 63 4460.0 64 2526.5 65 1416.5 66 754.0 67 374.0 68 201.0 69 122.0 70 77.5 71 44.5 72 29.5 73 24.5 74 17.0 75 11.0 76 7.5 77 3.5 78 2.5 79 2.0 80 1.5 81 1.5 82 1.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004999796791884774 2 0.007017258655276876 3 0.0017835532415495393 4 0.17130882692194674 5 0.0024560405293469066 6 5.262943991457657E-4 7 0.0 8 5.262943991457657E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.508629327638438E-4 24-25 5.847715546064064E-4 26-27 0.00274842630665011 28-29 1.6081217751676174E-4 30-31 0.006330152078614348 32-33 0.020964060232639668 34-35 0.013493603622542826 36-37 9.063959096399298E-4 38-39 2.3390862184256256E-4 40-41 0.0018712689747405005 42-43 0.011417664603690083 44-45 0.00745583732123168 46-47 0.010642842293836597 48-49 0.010876750915679158 50-51 0.18703918174085907 52-53 0.11240771208421646 54-55 0.017089948683372224 56-57 0.006724872877973673 58-59 0.017937867437551516 60-61 0.12299207722259241 62-63 0.23801664201367254 64-65 0.24411288547044435 66-67 0.2543610069649216 68-69 0.24966821523920518 70-71 0.2344349162417083 72-73 0.15875085778677417 74-75 0.2650330878364885 76-77 0.2057518714882641 78-79 0.005759999812873102 80-81 2.923857773032032E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 2.923857773032032E-5 90-91 0.005876954123794384 92-93 0.017689339526843793 94-95 0.0026314719957288286 96-97 1.461928886516016E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3420139.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.197880152001886 #Duplication Level Percentage of deduplicated Percentage of total 1 70.43233103728757 36.05986043273172 2 16.158654061515612 16.545776681182694 3 5.6602827882640625 8.693834394599476 4 2.7575121613390428 5.647151086156961 5 1.462880669999691 3.744819460966221 6 0.8967514317915041 2.754706339859851 7 0.5589842617654754 2.0033166468506844 8 0.3649721517151603 1.494864038586482 9 0.2663532995447987 1.2273051877366377 >10 1.2910483684132084 11.711951381623923 >50 0.0932405852019269 3.356724957611997 >100 0.053894110013135835 5.069730212002145 >500 0.0025219114572141354 0.8908159564630194 >1k 4.5852935326036334E-4 0.44240014832804947 >5k 1.1463233831509084E-4 0.35674307530008603 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6836 0.1998749173644697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5321 0.1555784721030344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.9238577730320317E-5 0.0 16-17 0.0 0.0 0.0 5.8477155460640633E-5 0.0 18-19 0.0 0.0 0.0 5.8477155460640633E-5 0.0 20-21 0.0 0.0 0.0 8.771573319096094E-5 0.0 22-23 0.0 0.0 0.0 1.754314663819219E-4 0.0 24-25 0.0 0.0 0.0 3.65482221629004E-4 0.0 26-27 0.0 0.0 0.0 5.847715546064064E-4 2.9238577730320317E-5 28-29 0.0 0.0 0.0 0.001213400975808293 2.9238577730320317E-5 30-31 0.0 0.0 0.0 0.004210355193166126 2.9238577730320317E-5 32-33 0.0 0.0 0.0 0.011505380336881045 2.9238577730320317E-5 34-35 0.0 1.4619288865160158E-5 0.0 0.02324466929560465 2.9238577730320317E-5 36-37 0.0 2.9238577730320317E-5 0.0 0.04280527779718894 2.9238577730320317E-5 38-39 0.0 2.9238577730320317E-5 0.0 0.07486537827848516 2.9238577730320317E-5 40-41 0.0 2.9238577730320317E-5 0.0 0.1123930927953513 2.9238577730320317E-5 42-43 0.0 2.9238577730320317E-5 0.0 0.1515874062428457 2.9238577730320317E-5 44-45 0.0 2.9238577730320317E-5 0.0 0.1993486229653239 2.9238577730320317E-5 46-47 0.0 2.9238577730320317E-5 0.0 0.2580889256255374 2.9238577730320317E-5 48-49 0.0 2.9238577730320317E-5 0.0 0.32450435493996005 2.9238577730320317E-5 50-51 0.0 2.9238577730320317E-5 0.0 0.3988580581081646 2.9238577730320317E-5 52-53 0.0 2.9238577730320317E-5 0.0 0.4727878018992795 4.385786659548047E-5 54-55 0.0 2.9238577730320317E-5 0.0 0.555255210387648 5.8477155460640633E-5 56-57 0.0 2.9238577730320317E-5 0.0 0.6566253593786686 5.8477155460640633E-5 58-59 0.0 2.9238577730320317E-5 0.0 0.7724247464796021 5.8477155460640633E-5 60-61 0.0 2.9238577730320317E-5 0.0 0.8980190571201931 5.8477155460640633E-5 62-63 0.0 2.9238577730320317E-5 0.0 1.0265957026892767 5.8477155460640633E-5 64-65 0.0 2.9238577730320317E-5 0.0 1.1662976270847472 5.8477155460640633E-5 66-67 0.0 2.9238577730320317E-5 0.0 1.3227678758085561 5.8477155460640633E-5 68-69 0.0 2.9238577730320317E-5 0.0 1.5085059408404162 5.8477155460640633E-5 70-71 0.0 2.9238577730320317E-5 0.0 1.7141993351732197 5.8477155460640633E-5 72-73 1.4619288865160158E-5 2.9238577730320317E-5 0.0 1.925901257229604 8.771573319096096E-5 74-75 2.9238577730320317E-5 2.9238577730320317E-5 0.0 2.1437286613204902 8.771573319096096E-5 76-77 2.9238577730320317E-5 2.9238577730320317E-5 0.0 2.3839235773750715 8.771573319096096E-5 78-79 2.9238577730320317E-5 2.9238577730320317E-5 0.0 2.658722350173487 8.771573319096096E-5 80-81 2.9238577730320317E-5 2.9238577730320317E-5 0.0 2.9682419340266577 8.771573319096096E-5 82-83 2.9238577730320317E-5 2.9238577730320317E-5 0.0 3.2941205021199433 8.771573319096096E-5 84-85 2.9238577730320317E-5 2.9238577730320317E-5 0.0 3.6082890198322346 8.771573319096096E-5 86-87 2.9238577730320317E-5 2.9238577730320317E-5 0.0 3.9506581457654204 8.771573319096096E-5 88-89 2.9238577730320317E-5 2.9238577730320317E-5 0.0 4.338288590025142 8.771573319096096E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4240 0.0 53.46495 1 GTATCAA 8145 0.0 42.477306 1 ATCAACG 9920 0.0 34.489983 3 CAACGCA 9975 0.0 34.24816 5 TCAACGC 10160 0.0 33.53446 4 TATCAAC 10540 0.0 32.59928 2 AACGCAG 10725 0.0 32.030148 6 CGCAGAG 12315 0.0 27.354906 8 ACGCAGA 12355 0.0 27.304749 7 GCAGAGT 14075 0.0 23.124605 9 ACGGGCG 480 0.0 21.746988 5 GTACATG 9470 0.0 21.729744 1 TACGGGC 560 0.0 21.213766 4 TACATGG 9535 0.0 21.132725 2 ACATGGG 9080 0.0 21.09072 3 GCGTCGG 500 0.0 20.876194 9 GGCGTCG 505 0.0 20.670103 8 TAACGCC 235 1.1287739E-7 20.220781 4 AGAGTAC 13105 0.0 20.075352 10-11 GAGTACT 9840 0.0 19.552143 12-13 >>END_MODULE