FastQCFastQC Report
Sat 18 Jun 2016
SRR1931023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868568
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN830804.446185528169165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17450.057.552191
GTATCAA31500.043.2685431
ATCAACG37600.034.9633453
TCAACGC38850.033.9625784
CAACGCA39250.033.0912485
TATCAAC40150.032.916292
AACGCAG44650.029.5361146
GTACATG33300.027.0813081
ACGCAGA50200.026.0525937
CGCAGAG49850.025.3450188
ACATGGG35500.025.1133843
TACATGG36100.024.1222842
GTGGTAT8300.024.0761891
GCAGAGT53250.022.8938649
GAGTACT34050.022.49353212-13
GAGTACA24700.022.4041271
TGGTATC9550.021.9916212
CAGAGTA51100.021.23647310-11
CATGGGG22700.020.6322924
AGAGTAC46750.020.47008310-11
Produced by FastQC (version 0.11.5)