##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931023_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868568 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.82295854365482 28.0 2.0 31.0 2.0 33.0 2 19.558190015027552 27.0 2.0 31.0 2.0 33.0 3 20.177471732364037 27.0 2.0 31.0 2.0 33.0 4 21.696104717623335 30.0 2.0 35.0 2.0 37.0 5 21.03914387916308 30.0 2.0 35.0 2.0 37.0 6 21.993469865693942 30.0 2.0 35.0 2.0 37.0 7 22.16732064340179 30.0 2.0 35.0 2.0 37.0 8 22.581764752473553 30.0 2.0 35.0 2.0 37.0 9 23.833206498238223 32.0 2.0 37.0 2.0 39.0 10-11 24.163494451365963 32.0 2.0 37.0 2.0 39.0 12-13 24.051919437772668 32.0 2.0 37.0 2.0 39.0 14-15 23.981484216790612 32.0 2.0 38.0 2.0 40.0 16-17 22.83676457051603 30.5 2.0 37.5 2.0 40.0 18-19 21.808223463101157 28.0 2.0 37.0 2.0 40.0 20-21 21.47632331282565 27.0 2.0 37.0 2.0 40.0 22-23 21.116130373633712 27.0 2.0 37.0 2.0 39.0 24-25 20.942411782712753 27.0 2.0 37.0 2.0 39.0 26-27 20.651540377444118 25.5 2.0 36.5 2.0 39.0 28-29 20.440752490677355 25.0 2.0 36.0 2.0 39.0 30-31 20.403354600956455 25.0 2.0 36.0 2.0 39.0 32-33 20.251750270795604 25.0 2.0 36.0 2.0 39.0 34-35 19.912908708700996 24.0 2.0 36.0 2.0 39.0 36-37 19.444049132811866 23.5 2.0 35.0 2.0 39.0 38-39 19.3003433645444 23.0 2.0 35.0 2.0 39.0 40-41 19.14729247209628 22.5 2.0 35.0 2.0 38.0 42-43 18.978683141314633 22.0 2.0 35.0 2.0 38.0 44-45 18.5766156222305 19.0 2.0 35.0 2.0 38.0 46-47 18.22227582833485 16.5 2.0 34.0 2.0 38.0 48-49 17.958879473479158 15.0 2.0 34.0 2.0 38.0 50-51 15.79241563593083 9.0 2.0 32.0 2.0 37.5 52-53 17.62210500233334 16.0 2.0 33.5 2.0 38.0 54-55 18.349022888115393 18.5 2.0 35.0 2.0 38.5 56-57 18.299345541612617 17.5 2.0 35.0 2.0 39.0 58-59 18.01160460844882 14.5 2.0 35.0 2.0 39.0 60-61 17.718651395079014 13.0 2.0 34.0 2.0 38.5 62-63 17.276059795522563 8.0 2.0 34.0 2.0 38.0 64-65 16.914053167987465 8.0 2.0 34.0 2.0 38.0 66-67 16.551725974114937 6.5 2.0 33.0 2.0 38.0 68-69 16.101183366085685 2.0 2.0 32.5 2.0 37.0 70-71 15.749415327673384 2.0 2.0 32.0 2.0 36.5 72-73 15.346295130816753 2.0 2.0 31.5 2.0 36.0 74-75 14.896073356709524 2.0 2.0 31.0 2.0 36.0 76-77 14.480120070556705 2.0 2.0 31.0 2.0 35.0 78-79 14.101508213776539 2.0 2.0 30.5 2.0 35.0 80-81 13.669950732325503 2.0 2.0 30.0 2.0 34.0 82-83 13.212827951672082 2.0 2.0 29.5 2.0 34.0 84-85 12.80600973579768 2.0 2.0 29.0 2.0 34.0 86-87 12.32444684913795 2.0 2.0 29.0 2.0 34.0 88-89 11.900602761044823 2.0 2.0 27.5 2.0 34.0 90-91 11.425063471064473 2.0 2.0 26.5 2.0 34.0 92-93 10.879844618980952 2.0 2.0 26.0 2.0 33.0 94-95 10.319945541184481 2.0 2.0 25.0 2.0 33.0 96-97 9.697692296988924 2.0 2.0 21.5 2.0 32.0 98-99 9.04377496564214 2.0 2.0 10.0 2.0 32.0 100-101 8.163364940424968 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.799476479925577 1101 2 9.292050243300444 1101 3 8.6726812173589 1101 4 10.84776854586664 1101 5 11.671936402085791 1101 6 10.663899686726012 1101 7 10.51278116971002 1101 8 10.01132330787254 1101 9 9.85631550695955 1101 10-11 9.088685459434302 1101 12-13 9.06243043926014 1101 14-15 9.560122282086287 1101 16-17 10.733192089084355 1101 18-19 11.667781603876819 1101 20-21 11.58223584862575 1101 22-23 11.776053582286988 1101 24-25 11.855820622307615 1101 26-27 12.07711247385642 1101 28-29 11.963750603358129 1101 30-31 11.501511527858014 1101 32-33 11.1853448121587 1101 34-35 11.10344317088175 1101 36-37 11.558468126912516 1101 38-39 11.173638041002906 1101 40-41 10.947693403352897 1101 42-43 10.68697899346044 1101 44-45 10.861333498741644 1101 46-47 10.340308852994013 1101 48-49 10.62719118463652 1101 50-51 12.258561896701586 1101 52-53 11.420641594598209 1101 54-55 11.01516781922323 1101 56-57 11.021258468195523 1101 58-59 10.778689376422086 1101 60-61 10.473202993895288 1101 62-63 10.633960875380264 1101 64-65 10.403247435447454 1101 66-67 10.203568267828711 1101 68-69 10.221779163674498 1101 70-71 9.7073209403903 1101 72-73 9.642485429777281 1101 74-75 9.506533746944635 1101 76-77 9.146740002735351 1101 78-79 8.68805967216637 1101 80-81 8.422705046030003 1101 82-83 8.066789575346219 1101 84-85 7.659111253027374 1101 86-87 7.549096326433062 1101 88-89 7.187272976619951 1101 90-91 6.759238896136019 1101 92-93 6.498569505644641 1101 94-95 6.245145660155 1101 96-97 6.290032383580435 1101 98-99 5.965389605769385 1101 100-101 5.447497464551548 1102 1 7.543066223515321 1102 2 8.863291188417545 1102 3 8.405449476213011 1102 4 10.27091338394753 1102 5 11.096068037056984 1102 6 10.181649043021647 1102 7 9.976821933864752 1102 8 9.292930571909842 1102 9 9.311476181412363 1102 10-11 8.391670256214212 1102 12-13 8.612139351765052 1102 14-15 8.963451361938095 1102 16-17 9.716639092111578 1102 18-19 10.82708273954556 1102 20-21 11.075332599636901 1102 22-23 11.155072337651188 1102 24-25 10.96288700259484 1102 26-27 10.837922323750728 1102 28-29 10.758355743901603 1102 30-31 10.918396280154951 1102 32-33 10.87664879646952 1102 34-35 10.575342593901855 1102 36-37 10.746967843888239 1102 38-39 10.626551973481867 1102 40-41 10.077961280692445 1102 42-43 9.80280789743674 1102 44-45 9.73072442291603 1102 46-47 9.903925594827168 1102 48-49 9.378198545432408 1102 50-51 11.178018219507287 1102 52-53 10.391583037253593 1102 54-55 9.90174069824577 1102 56-57 9.645418730287911 1102 58-59 9.986434744402356 1102 60-61 10.020156343052005 1102 62-63 9.906350233450894 1102 64-65 9.774673780408051 1102 66-67 9.45806364582035 1102 68-69 9.153688960643812 1102 70-71 8.913308500309732 1102 72-73 8.709639975103919 1102 74-75 8.302294524866364 1102 76-77 8.020895394429049 1102 78-79 7.508503901230961 1102 80-81 7.119012823840251 1102 82-83 7.146068707635989 1102 84-85 7.118714542819346 1102 86-87 7.150437282481505 1102 88-89 6.705085783387425 1102 90-91 6.2795065191325286 1102 92-93 5.8901062679551135 1102 94-95 5.833514579868099 1102 96-97 5.840306262333485 1102 98-99 5.692003845299922 1102 100-101 4.952223104170185 1103 1 7.982792470213472 1103 2 9.145344440251993 1103 3 8.407318442297107 1103 4 10.402880708468054 1103 5 11.242670940051788 1103 6 10.479507118711822 1103 7 9.972249708989288 1103 8 9.405643624104979 1103 9 9.467927821176264 1103 10-11 8.944265884262563 1103 12-13 8.748730742272592 1103 14-15 9.268372990533326 1103 16-17 10.016806783334097 1103 18-19 11.00226486838335 1103 20-21 11.254681894302315 1103 22-23 11.295757027117556 1103 24-25 11.331204132670901 1103 26-27 11.268088854591895 1103 28-29 10.910485366020193 1103 30-31 10.504661635733939 1103 32-33 10.590701686222008 1103 34-35 10.668299616277334 1103 36-37 10.873203770906699 1103 38-39 10.621341941770144 1103 40-41 10.194474850439157 1103 42-43 9.87967604427011 1103 44-45 9.532807952108485 1103 46-47 9.564943012903438 1103 48-49 9.422453260959006 1103 50-51 11.119981692206359 1103 52-53 10.502964068349126 1103 54-55 10.01954693819652 1103 56-57 9.940227178723731 1103 58-59 9.73768752649724 1103 60-61 9.892778165635068 1103 62-63 9.645598440634839 1103 64-65 9.343536638910827 1103 66-67 9.339604825659585 1103 68-69 9.531523501006554 1103 70-71 8.996521676869815 1103 72-73 8.802162794594246 1103 74-75 8.587707107590537 1103 76-77 8.300904951458834 1103 78-79 7.78658862196316 1103 80-81 7.466175825725333 1103 82-83 7.012838796872538 1103 84-85 6.466469285475233 1103 86-87 6.302983513740138 1103 88-89 6.001628491599327 1103 90-91 5.458835533984875 1103 92-93 5.363709875236937 1103 94-95 5.178429745783831 1103 96-97 4.7814982963154655 1103 98-99 4.015289583299779 1103 100-101 3.3420424768443855 1104 1 8.067646905990742 1104 2 9.491288905994914 1104 3 9.06020821094349 1104 4 11.099846066712782 1104 5 11.750948397483086 1104 6 10.929885405740528 1104 7 10.415809179987281 1104 8 9.846621458910516 1104 9 9.864806072997286 1104 10-11 9.231865737172676 1104 12-13 9.07940211372933 1104 14-15 9.741197611920303 1104 16-17 10.72407456256365 1104 18-19 11.641565513336005 1104 20-21 11.711752249691308 1104 22-23 12.050729265204975 1104 24-25 11.768433522482585 1104 26-27 11.928774648160104 1104 28-29 11.650191942555182 1104 30-31 11.34136982139059 1104 32-33 11.379163084254031 1104 34-35 11.346153720841556 1104 36-37 11.677952766183651 1104 38-39 11.162551756108282 1104 40-41 10.82337592727276 1104 42-43 10.21759641752374 1104 44-45 10.5074641555724 1104 46-47 10.392527843867423 1104 48-49 10.24177954232319 1104 50-51 11.580597331199783 1104 52-53 10.96056175663853 1104 54-55 10.573501152556563 1104 56-57 10.373386429608487 1104 58-59 10.341450445762103 1104 60-61 10.117236032466561 1104 62-63 9.996043361469702 1104 64-65 9.959818107410381 1104 66-67 9.748115957808373 1104 68-69 9.422945995849275 1104 70-71 9.101302750260885 1104 72-73 8.91703934601442 1104 74-75 8.70862190759784 1104 76-77 8.368152023352932 1104 78-79 8.032471409055761 1104 80-81 7.990496773777046 1104 82-83 7.747085834862935 1104 84-85 7.530667502140725 1104 86-87 7.074856875604734 1104 88-89 6.761714189715583 1104 90-91 6.216770303270209 1104 92-93 5.978629719071288 1104 94-95 5.794525004742884 1104 96-97 5.3978727391864325 1104 98-99 4.7821090774044475 1104 100-101 4.2479790143379645 1105 1 7.907747156617308 1105 2 9.130887903488649 1105 3 8.58101021595602 1105 4 10.555485164457142 1105 5 10.83114889873622 1105 6 10.470737536066338 1105 7 9.887989631115104 1105 8 9.705268075451869 1105 9 9.486861210841898 1105 10-11 8.964447190806766 1105 12-13 8.64030436936843 1105 14-15 9.470270293624562 1105 16-17 10.30101691845337 1105 18-19 10.763620651180617 1105 20-21 11.164885081771509 1105 22-23 11.321405956934296 1105 24-25 11.25013778313421 1105 26-27 11.372884923849323 1105 28-29 11.076257105462453 1105 30-31 10.77370064845826 1105 32-33 10.990942532875586 1105 34-35 10.92309607673128 1105 36-37 10.997000385231267 1105 38-39 10.936737219767426 1105 40-41 10.807837080154965 1105 42-43 10.451431003989907 1105 44-45 10.132777438780423 1105 46-47 10.032878721060406 1105 48-49 10.079122900719181 1105 50-51 11.69613617831758 1105 52-53 10.815448974683644 1105 54-55 10.422373333007688 1105 56-57 10.330529286751347 1105 58-59 10.183054455787168 1105 60-61 10.138038037479088 1105 62-63 9.92461479004113 1105 64-65 9.644028633726172 1105 66-67 9.386712449036445 1105 68-69 9.156028702616194 1105 70-71 8.936139842992716 1105 72-73 8.765410273332712 1105 74-75 8.519900103086565 1105 76-77 8.476172073478244 1105 78-79 8.023448852664783 1105 80-81 7.606035620894836 1105 82-83 7.580669794762686 1105 84-85 7.079790309158268 1105 86-87 6.8690924645771645 1105 88-89 6.838656545605307 1105 90-91 6.303487095250155 1105 92-93 5.972865308043717 1105 94-95 5.46871046840203 1105 96-97 5.248499305602472 1105 98-99 4.907171734046051 1105 100-101 4.203367485515908 1106 1 7.883565100267976 1106 2 9.164458991329095 1106 3 8.599305955304391 1106 4 10.224843605877915 1106 5 11.125548795759745 1106 6 10.390219776410405 1106 7 9.47083344760497 1106 8 9.417700970378004 1106 9 9.114856425464556 1106 10-11 8.746223762144467 1106 12-13 8.314710550985609 1106 14-15 8.877670591953464 1106 16-17 10.249965952670337 1106 18-19 11.091439821462814 1106 20-21 11.309412563618118 1106 22-23 11.575753684258654 1106 24-25 11.075882280328859 1106 26-27 11.428767454950492 1106 28-29 11.252690636419754 1106 30-31 10.975301326891504 1106 32-33 11.005280113988487 1106 34-35 10.798739490401406 1106 36-37 11.198876298579595 1106 38-39 10.816890018683146 1106 40-41 10.761226974831235 1106 42-43 10.558521615715595 1106 44-45 10.037198991150774 1106 46-47 9.887937061776245 1106 48-49 9.986798055373182 1106 50-51 11.792427889468566 1106 52-53 11.066284522768356 1106 54-55 10.39243368025042 1106 56-57 10.369521783097952 1106 58-59 9.928026402269687 1106 60-61 9.993560908099752 1106 62-63 10.072734333613052 1106 64-65 10.012778302459939 1106 66-67 9.651491466065423 1106 68-69 9.408717448650464 1106 70-71 9.13214534011922 1106 72-73 8.783587135427528 1106 74-75 8.503735068142708 1106 76-77 8.424462361130912 1106 78-79 7.978092258763111 1106 80-81 7.81516531825001 1106 82-83 7.5288423409317335 1106 84-85 7.211150052928572 1106 86-87 6.8744722281855815 1106 88-89 6.500174767141019 1106 90-91 6.4197096758708945 1106 92-93 6.19064200053022 1106 94-95 5.943218490723698 1106 96-97 5.209957709921426 1106 98-99 4.871798936493258 1106 100-101 4.1857944744383655 1107 1 7.193645674398109 1107 2 8.104060977731386 1107 3 7.994654792513693 1107 4 9.720179002185944 1107 5 10.386172453578837 1107 6 9.361567746070623 1107 7 9.260755215450057 1107 8 7.9579452689411525 1107 9 5.342965830327923 1107 10-11 7.4297511230336895 1107 12-13 7.419784287566351 1107 14-15 7.863858029394731 1107 16-17 8.88437369259297 1107 18-19 10.260746301504604 1107 20-21 10.254483825851118 1107 22-23 9.699132502823794 1107 24-25 10.127274380948261 1107 26-27 10.228671128491364 1107 28-29 10.24161767574559 1107 30-31 10.018080049944786 1107 32-33 10.398175732152847 1107 34-35 10.41222499102916 1107 36-37 10.495075578269478 1107 38-39 10.44281624442716 1107 40-41 9.949498108357421 1107 42-43 9.625967192793563 1107 44-45 9.040994518385396 1107 46-47 9.167778153666188 1107 48-49 9.31574960397884 1107 50-51 10.640636838461365 1107 52-53 9.689154725410308 1107 54-55 9.482095692495893 1107 56-57 9.001435064846735 1107 58-59 9.000363907373664 1107 60-61 8.64591148753497 1107 62-63 8.678115072597876 1107 64-65 8.976644424973756 1107 66-67 8.951703414823005 1107 68-69 8.423570408245283 1107 70-71 8.070171839059732 1107 72-73 8.112151494434002 1107 74-75 8.02072788031528 1107 76-77 7.742303782933883 1107 78-79 7.449871202776665 1107 80-81 7.2317314327196485 1107 82-83 7.074642466583795 1107 84-85 6.843242037569288 1107 86-87 6.504786857282895 1107 88-89 6.311871813299316 1107 90-91 5.982506686695913 1107 92-93 5.751855123330168 1107 94-95 5.395732142911864 1107 96-97 5.075920867596436 1107 98-99 4.859241367178427 1107 100-101 4.0175569024353575 1108 1 6.521642642051376 1108 2 7.900466238832561 1108 3 7.85813542049107 1108 4 9.156421157315172 1108 5 10.691773214318314 1108 6 9.184953955014823 1108 7 8.412928618340988 1108 8 5.845451531063652 1108 9 6.86642060377428 1108 10-11 6.095874902366184 1108 12-13 4.886313944264295 1108 14-15 7.012301962099485 1108 16-17 8.175636616032335 1108 18-19 7.795246132644113 1108 20-21 8.001352764601187 1108 22-23 8.736713145187473 1108 24-25 8.383564816914426 1108 26-27 6.944461063997881 1108 28-29 9.096055339695866 1108 30-31 9.458302415236453 1108 32-33 10.229207342224893 1108 34-35 9.723682558070855 1108 36-37 9.31640459707268 1108 38-39 8.58015492143836 1108 40-41 8.98900202581882 1108 42-43 8.330698041411917 1108 44-45 7.812049534256246 1108 46-47 8.853256355358056 1108 48-49 9.379625039011426 1108 50-51 10.667492798930667 1108 52-53 9.729008352441863 1108 54-55 9.25780993156766 1108 56-57 9.411631891901962 1108 58-59 9.365988961577376 1108 60-61 9.02493059682903 1108 62-63 9.266589808313348 1108 64-65 9.068284657236006 1108 66-67 8.397283402150816 1108 68-69 8.755306538983096 1108 70-71 8.837976928137465 1108 72-73 8.491180551702705 1108 74-75 8.219959029413479 1108 76-77 7.717648620547228 1108 78-79 7.5948124281018625 1108 80-81 7.576509358214931 1108 82-83 7.333608397576478 1108 84-85 7.063894236989958 1108 86-87 6.621889568335554 1108 88-89 6.795971123675102 1108 90-91 6.4512970844006325 1108 92-93 6.192175692986906 1108 94-95 5.994825958944734 1108 96-97 5.546202696268846 1108 98-99 5.20710699095725 1108 100-101 4.881739500315776 1109 1 6.538852309938857 1109 2 7.921573032646204 1109 3 7.726297987176903 1109 4 8.899581211347275 1109 5 10.07379959919254 1109 6 8.856406389474763 1109 7 7.5755249534847735 1109 8 8.079847951436651 1109 9 7.242196511244298 1109 10-11 5.708725216548409 1109 12-13 6.148227189366629 1109 14-15 7.389191226434679 1109 16-17 6.306247874329117 1109 18-19 5.533948487704194 1109 20-21 7.838501143839025 1109 22-23 8.947581829883603 1109 24-25 8.377651537639998 1109 26-27 8.690681781531378 1109 28-29 9.308956258724091 1109 30-31 6.598461255154284 1109 32-33 5.966171192173231 1109 34-35 7.43118252791901 1109 36-37 5.974560570555134 1109 38-39 6.1676864803691345 1109 40-41 7.321995983389147 1109 42-43 5.9540668266141985 1109 44-45 6.7284859580866545 1109 46-47 6.024667331031548 1109 48-49 6.750888220392863 1109 50-51 8.358123816736335 1109 52-53 8.892802218350578 1109 54-55 8.970762108731861 1109 56-57 7.910088368719212 1109 58-59 7.784859141804997 1109 60-61 7.8024697841765125 1109 62-63 8.19339243634813 1109 64-65 8.311919351556604 1109 66-67 8.045103072008214 1109 68-69 7.582438726680337 1109 70-71 7.390103437237293 1109 72-73 6.702423873069108 1109 74-75 5.88443357321607 1109 76-77 5.9136190275167255 1109 78-79 6.517501339459459 1109 80-81 6.49598634431694 1109 82-83 6.294402707722375 1109 84-85 6.071078165252688 1109 86-87 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-15.996037129288755 2313 58-59 -15.707421734689024 2313 60-61 -15.425119857257751 2313 62-63 -14.982653380384935 2313 64-65 -14.615620489080847 2313 66-67 -14.263412864246764 2313 68-69 -13.826176811168267 2313 70-71 -13.48240652292207 2313 72-73 -13.078950628922925 2313 74-75 -12.635237741524966 2313 76-77 -12.224329179654587 2313 78-79 -11.856751648588348 2313 80-81 -11.430806484368318 2313 82-83 -10.982738726540573 2313 84-85 -10.573593149488348 2313 86-87 -10.095819816124589 2313 88-89 -9.691919895497449 2313 90-91 -9.231851250614504 2313 92-93 -8.694302611755779 2313 94-95 -8.128282012800977 2313 96-97 -7.524433025224596 2313 98-99 -6.898342050415101 2313 100-101 -6.029965847817424 2314 1 -18.533856853407755 2314 2 -17.277132146636664 2314 3 -17.90069404469561 2314 4 -19.371833131282205 2314 5 -18.688650600010646 2314 6 -19.650565722079023 2314 7 -19.837323652092916 2314 8 -20.282701849360162 2314 9 -21.535193927002712 2314 10-11 -21.880916217714542 2314 12-13 -21.789770818601497 2314 14-15 -21.70717331539799 2314 16-17 -20.544847743200762 2314 18-19 -19.51958736886951 2314 20-21 -19.17195702349165 2314 22-23 -18.80465920597297 2314 24-25 -18.63081459781817 2314 26-27 -18.347415828030375 2314 28-29 -18.13451983689344 2314 30-31 -18.10173794803297 2314 32-33 -17.950661477149477 2314 34-35 -17.635550539810072 2314 36-37 -17.161395604743667 2314 38-39 -17.005869296523297 2314 40-41 -16.85131078951922 2314 42-43 -16.6798973168121 2314 44-45 -16.276245117610557 2314 46-47 -15.927772908012276 2314 48-49 -15.65897233737606 2314 50-51 -13.552234749000718 2314 52-53 -15.313373080454202 2314 54-55 -16.026498847443438 2314 56-57 -15.996037129288755 2314 58-59 -15.707421734689024 2314 60-61 -15.425119857257751 2314 62-63 -14.982653380384935 2314 64-65 -14.615620489080847 2314 66-67 -14.263412864246764 2314 68-69 -13.826176811168267 2314 70-71 -13.48240652292207 2314 72-73 -13.078950628922925 2314 74-75 -12.635237741524966 2314 76-77 -12.224329179654587 2314 78-79 -11.856751648588348 2314 80-81 -11.430806484368318 2314 82-83 -10.982738726540573 2314 84-85 -10.573593149488348 2314 86-87 -10.095819816124589 2314 88-89 -9.691919895497449 2314 90-91 -9.231851250614504 2314 92-93 -8.694302611755779 2314 94-95 -8.128282012800977 2314 96-97 -7.524433025224596 2314 98-99 -6.898342050415101 2314 100-101 -6.029965847817424 2315 1 -18.533856853407755 2315 2 -17.277132146636664 2315 3 -17.90069404469561 2315 4 -19.371833131282205 2315 5 -18.688650600010646 2315 6 -19.650565722079023 2315 7 -19.837323652092916 2315 8 -20.282701849360162 2315 9 -21.535193927002712 2315 10-11 -21.880916217714542 2315 12-13 -21.789770818601497 2315 14-15 -21.70717331539799 2315 16-17 -20.544847743200762 2315 18-19 -19.51958736886951 2315 20-21 -19.17195702349165 2315 22-23 -18.80465920597297 2315 24-25 -18.63081459781817 2315 26-27 -18.347415828030375 2315 28-29 -18.13451983689344 2315 30-31 -18.10173794803297 2315 32-33 -17.950661477149477 2315 34-35 -17.635550539810072 2315 36-37 -17.161395604743667 2315 38-39 -17.005869296523297 2315 40-41 -16.85131078951922 2315 42-43 -16.6798973168121 2315 44-45 -16.276245117610557 2315 46-47 -15.927772908012276 2315 48-49 -15.65897233737606 2315 50-51 -13.552234749000718 2315 52-53 -15.313373080454202 2315 54-55 -16.026498847443438 2315 56-57 -15.996037129288755 2315 58-59 -15.707421734689024 2315 60-61 -15.425119857257751 2315 62-63 -14.982653380384935 2315 64-65 -14.615620489080847 2315 66-67 -14.263412864246764 2315 68-69 -13.826176811168267 2315 70-71 -13.48240652292207 2315 72-73 -13.078950628922925 2315 74-75 -12.635237741524966 2315 76-77 -12.224329179654587 2315 78-79 -11.856751648588348 2315 80-81 -11.430806484368318 2315 82-83 -10.982738726540573 2315 84-85 -10.573593149488348 2315 86-87 -10.095819816124589 2315 88-89 -9.691919895497449 2315 90-91 -9.231851250614504 2315 92-93 -8.694302611755779 2315 94-95 -8.128282012800977 2315 96-97 -7.524433025224596 2315 98-99 -6.898342050415101 2315 100-101 -6.029965847817424 2316 1 -18.533856853407755 2316 2 -17.277132146636664 2316 3 -17.90069404469561 2316 4 -19.371833131282205 2316 5 -18.688650600010646 2316 6 -19.650565722079023 2316 7 -19.837323652092916 2316 8 -20.282701849360162 2316 9 -21.535193927002712 2316 10-11 -21.880916217714542 2316 12-13 -21.789770818601497 2316 14-15 -21.70717331539799 2316 16-17 -20.544847743200762 2316 18-19 -19.51958736886951 2316 20-21 -19.17195702349165 2316 22-23 -18.80465920597297 2316 24-25 -18.63081459781817 2316 26-27 -18.347415828030375 2316 28-29 -18.13451983689344 2316 30-31 -18.10173794803297 2316 32-33 -17.950661477149477 2316 34-35 -17.635550539810072 2316 36-37 -17.161395604743667 2316 38-39 -17.005869296523297 2316 40-41 -16.85131078951922 2316 42-43 -16.6798973168121 2316 44-45 -16.276245117610557 2316 46-47 -15.927772908012276 2316 48-49 -15.65897233737606 2316 50-51 -13.552234749000718 2316 52-53 -15.313373080454202 2316 54-55 -16.026498847443438 2316 56-57 -15.996037129288755 2316 58-59 -15.707421734689024 2316 60-61 -15.425119857257751 2316 62-63 -14.982653380384935 2316 64-65 -14.615620489080847 2316 66-67 -14.263412864246764 2316 68-69 -13.826176811168267 2316 70-71 -13.48240652292207 2316 72-73 -13.078950628922925 2316 74-75 -12.635237741524966 2316 76-77 -12.224329179654587 2316 78-79 -11.856751648588348 2316 80-81 -11.430806484368318 2316 82-83 -10.982738726540573 2316 84-85 -10.573593149488348 2316 86-87 -10.095819816124589 2316 88-89 -9.691919895497449 2316 90-91 -9.231851250614504 2316 92-93 -8.694302611755779 2316 94-95 -8.128282012800977 2316 96-97 -7.524433025224596 2316 98-99 -6.898342050415101 2316 100-101 -6.029965847817424 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 490030.0 3 21069.0 4 72529.0 5 39871.0 6 24090.0 7 23858.0 8 23335.0 9 23281.0 10 24603.0 11 26021.0 12 26529.0 13 26830.0 14 27390.0 15 27956.0 16 29033.0 17 29356.0 18 28598.0 19 29917.0 20 30922.0 21 32565.0 22 34503.0 23 36528.0 24 39242.0 25 41674.0 26 44274.0 27 47167.0 28 50512.0 29 54401.0 30 58382.0 31 62822.0 32 67220.0 33 70296.0 34 71498.0 35 65385.0 36 46498.0 37 18294.0 38 2076.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50578592291771 21.305526751028356 14.358838766716381 22.829848559337556 2 14.860622493533837 20.97414671276608 41.848546012943686 22.3166847807564 3 18.425309196194295 26.70452884555154 30.268932829432277 24.60122912882189 4 12.03242680522823 17.429863072321428 39.89037287487424 30.647337247576107 5 12.37917385858069 38.30604708575568 35.97638325031356 13.33839580535007 6 29.228949387846757 39.24810890098487 18.043412495950324 13.479529215218044 7 25.84283367527514 33.145864619246034 23.549778971636457 17.46152273384237 8 24.329037728191466 37.049761779427975 20.828595041100918 17.79260545127964 9 25.236478115754036 16.818549503723894 21.916978812921155 36.027993567600916 10-11 23.842200593368037 27.552207961369625 29.121091807327076 19.48449963793526 12-13 24.442859316746556 24.979551852697256 29.504393973978544 21.073194856577647 14-15 21.840993995997334 26.69212808539026 27.113242161440958 24.35363575717145 16-17 20.96736359036005 29.67426932294433 28.005383368357013 21.352983718338603 18-19 20.67472492458231 28.476243336482927 30.362951983031845 20.486079755902917 20-21 21.43417151463193 27.87822158107459 30.261655992137964 20.42595091215551 22-23 21.234408235299863 27.689719138760836 30.041620622967958 21.034252002971346 24-25 21.026772912648912 27.918430060638542 30.32287916942902 20.731917857283527 26-27 20.867179670306356 28.164245275969446 30.131172274522662 20.837402779201536 28-29 20.89738621039407 28.12002944871544 30.217746164751443 20.76483817613905 30-31 21.215020374256635 27.916593221785803 30.13464710058087 20.73373930337669 32-33 21.001041376964974 28.211146196276776 29.877823190448055 20.909989236310196 34-35 21.214633489924093 28.037497989505304 30.132282562114078 20.615585958456524 36-37 21.2102069979057 27.931817324958104 29.995697554333162 20.862278122803033 38-39 21.230109501890453 28.07806962646413 30.086986790894716 20.6048340807507 40-41 21.272766391001376 28.000896164097604 30.050622602894393 20.675714842006627 42-43 21.131917459225686 28.0521286676015 29.758969485008706 21.0569843881641 44-45 21.06516315539363 28.42600161797651 29.597584915480528 20.911250311149328 46-47 21.248252498280237 28.10929559962424 29.430406899765522 21.21204500233 48-49 21.3785326357895 28.302740230003252 29.194668993820006 21.124058140387238 50-51 21.528773191527304 28.531994398292216 28.744381245818012 21.19485116436247 52-53 20.95977060486399 28.799511621000295 28.927174881480095 21.313542892655615 54-55 20.997325881386356 28.770585741871674 28.98332905962779 21.248759317114185 56-57 21.011629296825788 28.834331605276564 28.953535576875794 21.20050352102186 58-59 20.950246589949796 28.747358599546807 29.11346692140223 21.18892788910117 60-61 21.027774952104846 28.950502687214964 28.97271954065861 21.049002820021588 62-63 20.86923973244402 28.874736627663943 28.967749621286615 21.288274018605417 64-65 20.72768279706316 28.680538222256946 29.0952813249101 21.496497655769794 66-67 20.7600429398859 28.676397155667527 28.827835067689477 21.7357248367571 68-69 20.744387201486827 28.754703624919888 28.91097995446729 21.589929219125995 70-71 20.974730040409263 28.34786527665069 29.03888717617637 21.638517506763677 72-73 20.93503840200715 28.626448810840564 28.85221913733096 21.58629364982132 74-75 20.836408142695152 28.970970466497743 28.53383090248405 21.658790488323056 76-77 20.816935211638686 28.820179158055936 28.74872039761672 21.614165232688663 78-79 20.70317138190994 28.88677648885189 28.64699420627926 21.763057922958918 80-81 20.815580458845638 28.835205204668473 28.434433633465183 21.914780703020703 82-83 20.990798034337228 28.621887984472682 28.71691772732234 21.67039625386775 84-85 20.950389113189726 28.728072155210604 28.393476607233907 21.92806212436576 86-87 20.799008288469135 28.841833807176066 28.547027371279164 21.81213053307563 88-89 21.00885623560093 28.696535084757326 28.40405991154852 21.890548768093225 90-91 20.90659897612375 28.622060188931574 28.600049405010346 21.871291429934324 92-93 20.961226341703107 28.738379777874478 28.275121334865826 22.025272545556586 94-95 20.73433223843533 28.537356957280803 28.429314455178012 22.298996349105856 96-97 20.812589495160953 28.629585008116 28.389791109328215 22.16803438739483 98-99 20.670785536792597 28.6857701190499 28.143297649791432 22.50014669436608 100-101 20.980236116742688 28.701391564471106 27.960637114779253 22.35773520400695 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 112743.0 1 102188.5 2 67995.0 3 34721.0 4 21489.0 5 16868.5 6 15626.5 7 15707.0 8 16421.5 9 16412.0 10 15011.5 11 12677.5 12 10209.5 13 7987.0 14 6079.0 15 4952.0 16 4691.0 17 4990.0 18 5686.5 19 6634.5 20 7681.0 21 8778.0 22 9850.5 23 11172.5 24 12602.5 25 14247.0 26 16288.0 27 18551.0 28 21145.5 29 24316.5 30 27839.0 31 31154.0 32 34942.5 33 38957.5 34 42596.0 35 45576.0 36 48944.5 37 52442.0 38 53569.5 39 53565.0 40 53334.5 41 52499.5 42 51672.0 43 51469.0 44 52032.0 45 52176.0 46 51403.0 47 50333.0 48 49131.0 49 47662.0 50 45833.5 51 43095.5 52 40198.0 53 37300.0 54 33689.5 55 29600.0 56 24975.0 57 20487.0 58 16556.0 59 13098.5 60 10222.5 61 7657.0 62 5561.5 63 3992.0 64 2692.5 65 1751.0 66 1108.5 67 719.0 68 479.5 69 307.0 70 194.5 71 124.0 72 79.0 73 62.5 74 49.0 75 31.5 76 19.5 77 11.5 78 7.5 79 6.5 80 4.0 81 3.0 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.276417020948664 2 29.339740378728525 3 23.107427720050865 4 29.468876701302815 5 32.62557209585094 6 25.16938104473586 7 24.09320934533825 8 20.091000166972783 9 11.87561812040022 10-11 15.526381699782934 12-13 15.59525797295041 14-15 19.778140265700795 16-17 23.115080639291694 18-19 27.105623129583723 20-21 29.17991210381426 22-23 28.784475598426173 24-25 27.308264938712423 26-27 28.738504566063423 28-29 25.709099160426597 30-31 27.588640070899213 32-33 26.58862294548553 34-35 24.6355765484585 36-37 27.60664851372816 38-39 27.741190044997026 40-41 24.755614995012223 42-43 25.61646137577011 44-45 24.321084381194584 46-47 27.648873361847148 48-49 22.976284513060268 50-51 28.57669616519174 52-53 22.153863279259838 54-55 23.300490000899085 56-57 25.451441959832344 58-59 24.71842073716343 60-61 24.241879342897874 62-63 24.079883632814006 64-65 22.5975720444747 66-67 23.10194223597964 68-69 24.095831674308883 70-71 22.577182098805075 72-73 22.463752991595705 74-75 19.71964627458032 76-77 21.999948623758943 78-79 21.124786467498105 80-81 22.149260824331787 82-83 23.01607434142081 84-85 19.971470131137856 86-87 21.514469904226125 88-89 20.358236895847515 90-91 21.465930059810507 92-93 21.473368911380266 94-95 20.499200457248545 96-97 19.42128945802347 98-99 18.827733323058084 100-101 20.47827534240124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1868568.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.59268640576829 #Duplication Level Percentage of deduplicated Percentage of total 1 88.93888515723704 67.23129254969659 2 7.180336081949347 10.855617874616396 3 1.865266260962139 4.230014625845127 4 0.7326657393559908 2.215366859015511 5 0.3737468260936634 1.4126263310024756 6 0.2189979734626076 0.9932787078874596 7 0.14088381395972088 0.7454850177814046 8 0.09167399433211836 0.5543906804089601 9 0.07728049459773487 0.5257656174068318 >10 0.357649078619621 4.727049320047456 >50 0.01567355431481543 0.821274673387789 >100 0.006575852454664395 0.8433238381423782 >500 1.4606906422179983E-4 0.08032875282257598 >1k 1.4606906422179978E-4 0.1774409701524118 >5k 0.0 0.0 >10k+ 7.303453211089989E-5 4.586744181786675 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 83080 4.446185528169165 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.351691776804484E-5 0.0 22-23 0.0 0.0 0.0 5.351691776804484E-5 0.0 24-25 0.0 0.0 0.0 2.1406767107217935E-4 0.0 26-27 0.0 0.0 0.0 3.2110150660826897E-4 0.0 28-29 0.0 0.0 0.0 7.759953076366501E-4 0.0 30-31 0.0 0.0 0.0 0.002033642875185704 0.0 32-33 0.0 0.0 0.0 0.00508410718796426 0.0 34-35 0.0 0.0 0.0 0.011372345025709527 0.0 36-37 0.0 0.0 0.0 0.021380008648333913 0.0 38-39 0.0 0.0 0.0 0.03668584712999473 0.0 40-41 0.0 0.0 0.0 0.054078845404609305 0.0 42-43 0.0 0.0 0.0 0.07099019141931148 0.0 44-45 0.0 0.0 0.0 0.09223640777322527 0.0 46-47 0.0 0.0 0.0 0.11896810819836368 0.0 48-49 0.0 0.0 0.0 0.1502755050926699 0.0 50-51 0.0 0.0 0.0 0.18760355523588118 0.0 52-53 0.0 0.0 0.0 0.22717931592535034 0.0 54-55 0.0 0.0 0.0 0.2725884206515363 0.0 56-57 0.0 0.0 0.0 0.3268278168094498 0.0 58-59 0.0 0.0 0.0 0.39107487658998763 0.0 60-61 0.0 0.0 0.0 0.45946949749754895 0.0 62-63 0.0 0.0 0.0 0.5349551100093761 0.0 64-65 0.0 0.0 0.0 0.6062396444764119 0.0 66-67 0.0 0.0 0.0 0.6903414807488943 0.0 68-69 0.0 0.0 0.0 0.7890534355720531 0.0 70-71 0.0 0.0 0.0 0.8989771846676171 0.0 72-73 0.0 0.0 0.0 1.0092220352697894 0.0 74-75 0.0 0.0 0.0 1.116978349195748 0.0 76-77 0.0 0.0 0.0 1.2402813277333231 0.0 78-79 0.0 0.0 2.675845888402242E-5 1.382315227489714 0.0 80-81 0.0 0.0 5.351691776804484E-5 1.5424913623694723 0.0 82-83 0.0 0.0 5.351691776804484E-5 1.7053433431376326 0.0 84-85 0.0 5.351691776804484E-5 5.351691776804484E-5 1.8607029554182668 0.0 86-87 5.351691776804484E-5 5.351691776804484E-5 5.351691776804484E-5 2.025588579061613 0.0 88-89 5.351691776804484E-5 5.351691776804484E-5 5.351691776804484E-5 2.2136470280985225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1745 0.0 57.55219 1 GTATCAA 3150 0.0 43.268543 1 ATCAACG 3760 0.0 34.963345 3 TCAACGC 3885 0.0 33.962578 4 CAACGCA 3925 0.0 33.091248 5 TATCAAC 4015 0.0 32.91629 2 AACGCAG 4465 0.0 29.536114 6 GTACATG 3330 0.0 27.081308 1 ACGCAGA 5020 0.0 26.052593 7 CGCAGAG 4985 0.0 25.345018 8 ACATGGG 3550 0.0 25.113384 3 TACATGG 3610 0.0 24.122284 2 GTGGTAT 830 0.0 24.076189 1 GCAGAGT 5325 0.0 22.893864 9 GAGTACT 3405 0.0 22.493532 12-13 GAGTACA 2470 0.0 22.404127 1 TGGTATC 955 0.0 21.991621 2 CAGAGTA 5110 0.0 21.236473 10-11 CATGGGG 2270 0.0 20.632292 4 AGAGTAC 4675 0.0 20.470083 10-11 >>END_MODULE