FastQCFastQC Report
Sat 18 Jun 2016
SRR1931023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868568
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61510.3291825611912438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54700.29273754019120524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34330.18372357869769793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.061.076121
GTATCAA54800.043.6984331
ATCAACG69950.033.8926433
CAACGCA70850.033.81285
TCAACGC71650.033.219334
TATCAAC71700.033.000922
AACGCAG76950.031.7488696
GTGGTAT13000.028.5080261
CGCAGAG85000.027.9607268
ACGCAGA85350.027.9572287
ACATGGG53650.025.5044143
GTACATG55800.025.0339261
TGGTATC14050.025.024842
GCAGAGT94250.024.7125859
TACATGG56400.024.6833652
CTAGACG1050.00219450722.6229924
TAACGCA2955.456968E-1222.5463034
GAGTACT69200.021.2507212-13
AGAGTAC90050.020.8870710-11
CAGAGTA93900.020.83897810-11
Produced by FastQC (version 0.11.5)