##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931023_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1868568 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.332237842026622 31.0 31.0 34.0 30.0 34.0 2 31.4934061805618 31.0 31.0 34.0 30.0 34.0 3 31.550538701294254 31.0 31.0 34.0 30.0 34.0 4 35.3298622260469 37.0 35.0 37.0 33.0 37.0 5 35.202040814142165 37.0 35.0 37.0 33.0 37.0 6 35.23184010429377 37.0 35.0 37.0 32.0 37.0 7 35.110431089476 37.0 35.0 37.0 32.0 37.0 8 35.11617773610594 37.0 35.0 37.0 32.0 37.0 9 36.61563882074401 39.0 35.0 39.0 32.0 39.0 10-11 36.55824808088333 38.0 35.0 39.0 32.0 39.0 12-13 36.4389960654362 38.0 35.0 39.0 32.0 39.0 14-15 37.484901004405515 39.0 36.0 41.0 32.0 41.0 16-17 37.44065348437948 39.0 36.0 41.0 32.0 41.0 18-19 37.4801272953406 39.0 36.0 41.0 32.0 41.0 20-21 37.469890311725344 39.0 36.0 41.0 32.0 41.0 22-23 37.3652243322159 39.0 36.0 41.0 32.0 41.0 24-25 37.242725445367796 39.0 36.0 40.5 31.0 41.0 26-27 36.97214899323974 39.0 36.0 40.0 31.0 41.0 28-29 36.79311804547653 39.0 35.5 40.0 30.0 41.0 30-31 36.5576133702386 38.0 35.0 40.0 30.0 41.0 32-33 36.38525491178271 38.0 35.0 40.0 30.0 41.0 34-35 36.44864436295602 38.0 35.0 40.0 30.0 41.0 36-37 36.50863040574386 38.0 35.0 40.0 30.0 41.0 38-39 36.48161693874668 38.5 35.0 40.0 30.0 41.0 40-41 36.36706638452547 38.5 35.0 40.0 30.0 41.0 42-43 36.21732471068754 38.0 35.0 40.0 30.0 41.0 44-45 36.03276867633396 38.0 35.0 40.0 29.5 41.0 46-47 35.76762713478985 38.0 35.0 40.0 28.5 41.0 48-49 35.5212440756772 38.0 34.5 40.0 27.5 41.0 50-51 35.24531726969529 38.0 34.0 40.0 26.5 41.0 52-53 35.033126972098415 38.0 34.0 40.0 26.0 41.0 54-55 34.88226572434078 38.0 34.0 40.0 26.0 41.0 56-57 34.7037758326162 38.0 33.5 40.0 26.0 41.0 58-59 34.48842509344054 38.0 33.0 40.0 26.0 41.0 60-61 34.175387783586146 37.0 33.0 40.0 25.0 41.0 62-63 33.9146493464514 37.0 33.0 40.0 24.0 41.0 64-65 33.593349827247394 36.5 32.0 39.5 23.0 41.0 66-67 33.27395925650016 36.0 32.0 39.0 23.0 41.0 68-69 32.92420666521101 36.0 31.5 39.0 22.0 40.0 70-71 32.5648427030753 35.0 31.0 38.5 22.0 40.0 72-73 32.154653188966094 35.0 31.0 38.0 21.5 40.0 74-75 31.634784230490943 35.0 30.5 37.0 20.0 39.5 76-77 30.259239428268064 33.5 28.5 36.0 18.5 38.5 78-79 31.004205626982802 34.0 30.0 36.0 20.0 39.0 80-81 30.97687641017078 34.0 30.0 36.0 20.0 38.5 82-83 30.715334951684927 34.0 30.0 36.0 18.5 37.0 84-85 30.372761922498942 34.0 30.0 35.0 18.0 37.0 86-87 30.028499631803605 34.0 30.0 35.0 16.0 36.5 88-89 29.68563600575414 34.0 29.0 35.0 11.0 36.0 90-91 29.352407297995043 34.0 29.0 35.0 7.0 36.0 92-93 29.02581549079295 34.0 29.0 35.0 2.0 35.5 94-95 28.64563157455335 34.0 29.0 35.0 2.0 35.0 96-97 28.22448206326984 34.0 29.0 35.0 2.0 35.0 98-99 27.73255616065351 33.0 28.5 35.0 2.0 35.0 100-101 26.595093943597448 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3438515536243969 1101 2 0.4164835973123786 1101 3 0.2578550462713771 1101 4 0.13817681563168804 1101 5 0.2558445992560081 1101 6 0.3239863735912891 1101 7 0.42675720230541003 1101 8 0.4073864252752415 1101 9 0.45885724506307213 1101 10-11 0.4034304805428306 1101 12-13 0.42593639709059516 1101 14-15 0.450886770775611 1101 16-17 0.4925211206317641 1101 18-19 0.5007343010752763 1101 20-21 0.39140348006131376 1101 22-23 0.44760170821982825 1101 24-25 0.36557978127511603 1101 26-27 0.5309864018613197 1101 28-29 0.5550408107553721 1101 30-31 0.561566684967481 1101 32-33 0.5157469120184004 1101 34-35 0.586932606508725 1101 36-37 0.5340236032251084 1101 38-39 0.49276510349817215 1101 40-41 0.622836548193753 1101 42-43 0.4132769806045786 1101 44-45 0.3717151924932338 1101 46-47 0.37953660925568755 1101 48-49 0.40144336575941963 1101 50-51 0.45416520452765496 1101 52-53 0.4593947210540179 1101 54-55 0.2827635549029779 1101 56-57 0.28604695996654783 1101 58-59 0.3093547284020417 1101 60-61 0.34338776963303985 1101 62-63 0.2476391230600541 1101 64-65 0.18619227539500116 1101 66-67 0.1074714340159062 1101 68-69 0.27518584141945013 1101 70-71 0.22811158113920982 1101 72-73 0.17950001138668625 1101 74-75 0.18578650879888414 1101 76-77 0.15506046058856526 1101 78-79 0.19189392023029228 1101 80-81 0.21912439866128608 1101 82-83 0.3253595678985839 1101 84-85 0.4325680899332234 1101 86-87 0.3032712055625062 1101 88-89 0.36183062799093335 1101 90-91 0.3157902221430682 1101 92-93 0.18385553720866454 1101 94-95 0.4502487053217763 1101 96-97 0.577382160884504 1101 98-99 0.5112218334489 1101 100-101 0.7362885319071566 1102 1 0.3004075973021294 1102 2 0.4056861939925014 1102 3 0.33016504390111834 1102 4 0.1241695489968464 1102 5 0.2650926746367901 1102 6 0.30702575130585785 1102 7 0.4168061660469178 1102 8 0.3888158656447871 1102 9 0.5544095969786014 1102 10-11 0.41923384198377533 1102 12-13 0.4763342004325537 1102 14-15 0.5581556784750603 1102 16-17 0.47387696386553557 1102 18-19 0.5949027833183393 1102 20-21 0.49767057380782376 1102 22-23 0.46681908385419746 1102 24-25 0.5491697305039125 1102 26-27 0.599908225468468 1102 28-29 0.3368418084294831 1102 30-31 0.40707073876266264 1102 32-33 0.6685152195765056 1102 34-35 0.5924467095598658 1102 36-37 0.7526363188620024 1102 38-39 0.7118298191913581 1102 40-41 0.7726397299711039 1102 42-43 0.6701706240849035 1102 44-45 0.8213029132440468 1102 46-47 0.7959788317160204 1102 48-49 0.6976062221508457 1102 50-51 0.6672643582688238 1102 52-53 0.5652614950859132 1102 54-55 0.5650622802655292 1102 56-57 0.5735752759233819 1102 58-59 0.6205223361379737 1102 60-61 0.5001675269573056 1102 62-63 0.12334208393775725 1102 64-65 0.29524009621628267 1102 66-67 0.2109178904761606 1102 68-69 0.33373344523741366 1102 70-71 0.5002355024811962 1102 72-73 0.34021166863183083 1102 74-75 0.5185822042322883 1102 76-77 0.4489802278051549 1102 78-79 0.46893897358280157 1102 80-81 0.506174603813303 1102 82-83 0.34555183500423325 1102 84-85 0.606675914066372 1102 86-87 0.5531432223080159 1102 88-89 0.5108577929102083 1102 90-91 0.5122215186756698 1102 92-93 0.4348214996156834 1102 94-95 0.6084719947024695 1102 96-97 0.6723818578374754 1102 98-99 0.7587096547463972 1102 100-101 1.1200851483870693 1103 1 0.21625255101312035 1103 2 0.27782049524146757 1103 3 0.26926382512464286 1103 4 0.2124017778369378 1103 5 0.2723286731046599 1103 6 0.24144056722693819 1103 7 0.3011722835559425 1103 8 0.2428560073187569 1103 9 0.3194584270079801 1103 10-11 0.28989134307028763 1103 12-13 0.2967639766777168 1103 14-15 0.3523439138197375 1103 16-17 0.41351591335511273 1103 18-19 0.3844161859916184 1103 20-21 0.2884351469352211 1103 22-23 0.24417023942749694 1103 24-25 0.38371089906165423 1103 26-27 0.2795515102717161 1103 28-29 0.3429391826906212 1103 30-31 0.39307981659792546 1103 32-33 0.5428786886225225 1103 34-35 0.5168732474815059 1103 36-37 0.4626062510172986 1103 38-39 0.4570730614472893 1103 40-41 0.5355955758488165 1103 42-43 0.5799575544303224 1103 44-45 0.6261990241937028 1103 46-47 0.5196937765146359 1103 48-49 0.4603859962740202 1103 50-51 0.26099040190023004 1103 52-53 0.38343927195639793 1103 54-55 0.5376951425879 1103 56-57 0.46318271783582077 1103 58-59 0.5715859384666828 1103 60-61 0.488855017455343 1103 62-63 0.2894923092720063 1103 64-65 0.2206599420682167 1103 66-67 0.2132836448287989 1103 68-69 0.25218885409186953 1103 70-71 0.1473041696596411 1103 72-73 0.24391170038115462 1103 74-75 0.30308985066194083 1103 76-77 0.32901747819557414 1103 78-79 0.38552138725123797 1103 80-81 0.12252472632486189 1103 82-83 0.25840477761854075 1103 84-85 0.3230549386691415 1103 86-87 0.22088628594042703 1103 88-89 0.28344278950158497 1103 90-91 0.24012055153003686 1103 92-93 0.046053588243029964 1103 94-95 0.19635906381016355 1103 96-97 0.3621825086180195 1103 98-99 0.3766166222455105 1103 100-101 0.4259849086286458 1104 1 0.40577995954485857 1104 2 0.48478248919617783 1104 3 0.5075760468861219 1104 4 0.3003842209374099 1104 5 0.39957420629247054 1104 6 0.4715762602576419 1104 7 0.44488979794545713 1104 8 0.4339961886774617 1104 9 0.46199244154484376 1104 10-11 0.5697629784542784 1104 12-13 0.571481076398932 1104 14-15 0.5638389625758577 1104 16-17 0.5958588675146999 1104 18-19 0.6645002410825285 1104 20-21 0.49492131553049035 1104 22-23 0.5629762294285072 1104 24-25 0.538933763715491 1104 26-27 0.6234699692704098 1104 28-29 0.6954145438301964 1104 30-31 0.6917541820119837 1104 32-33 0.7033341978430272 1104 34-35 0.6227934851203472 1104 36-37 0.7607697137845264 1104 38-39 0.6932588693593686 1104 40-41 0.6037920816815614 1104 42-43 0.6918779641956263 1104 44-45 0.7161032853669198 1104 46-47 0.6213640552291153 1104 48-49 0.571118733423873 1104 50-51 0.5924204337957875 1104 52-53 0.4151299711288843 1104 54-55 0.6113038521870706 1104 56-57 0.5954840747661265 1104 58-59 0.644038923165688 1104 60-61 0.3875216893379658 1104 62-63 0.2670425845439439 1104 64-65 0.2852784341882142 1104 66-67 0.32457787937457283 1104 68-69 0.22918089193883162 1104 70-71 0.477390832727302 1104 72-73 0.4110265478536306 1104 74-75 0.41830569985829413 1104 76-77 0.4279766346715732 1104 78-79 0.5422241483162296 1104 80-81 0.4863167506103423 1104 82-83 0.5834233594529401 1104 84-85 0.7976009551150405 1104 86-87 0.8759560623585827 1104 88-89 0.8828816833021662 1104 90-91 0.7473502542989259 1104 92-93 0.8020827089115095 1104 94-95 0.8802915798035791 1104 96-97 0.98467200499163 1104 98-99 1.1566612968873713 1104 100-101 1.3331517591576691 1105 1 0.30402557357994553 1105 2 0.3419253463390355 1105 3 0.3276261721994054 1105 4 0.2121636695589615 1105 5 0.20759425641777796 1105 6 0.21242839058345453 1105 7 0.2674461889228965 1105 8 0.3382568403065349 1105 9 0.4775312886626324 1105 10-11 0.42164267770240116 1105 12-13 0.36371165284003126 1105 14-15 0.30769861169866886 1105 16-17 0.3537536043568039 1105 18-19 0.48780851175921924 1105 20-21 0.2771268293149518 1105 22-23 0.4516980339397847 1105 24-25 0.49808163338967404 1105 26-27 0.40968550811752635 1105 28-29 0.46007619796553456 1105 30-31 0.2634333800069655 1105 32-33 0.453208884559821 1105 34-35 0.5501117808596945 1105 36-37 0.572799788972489 1105 38-39 0.47396062374533443 1105 40-41 0.5461479714058726 1105 42-43 0.4665646809876094 1105 44-45 0.6133463931363465 1105 46-47 0.46422119808625695 1105 48-49 0.38816133994015445 1105 50-51 0.3513176769035553 1105 52-53 0.2990898708782552 1105 54-55 0.4584216466732869 1105 56-57 0.32606051586888896 1105 58-59 0.5184750133912459 1105 60-61 0.3905292081349572 1105 62-63 0.582080178528912 1105 64-65 0.47174459960174886 1105 66-67 0.5744525660913666 1105 68-69 0.5063738743949671 1105 70-71 0.48516025628619985 1105 72-73 0.5210516105102769 1105 74-75 0.6278295093821029 1105 76-77 0.5297310206364862 1105 78-79 0.639717882652068 1105 80-81 0.6381964498584622 1105 82-83 0.659613835643416 1105 84-85 0.5835658673957447 1105 86-87 0.6366076914312622 1105 88-89 0.7142100041041708 1105 90-91 0.8917111565545675 1105 92-93 0.860979952019278 1105 94-95 0.8529732840642303 1105 96-97 0.8170028320592948 1105 98-99 0.9709470111730845 1105 100-101 1.169993864420828 1106 1 0.32748225152859334 1106 2 0.31341146344448845 1106 3 0.28016688955169755 1106 4 0.13197301164529307 1106 5 0.18525139018876047 1106 6 0.07744137621975256 1106 7 0.336080009122945 1106 8 0.37502625629362996 1106 9 0.40289000266819386 1106 10-11 0.46729419319808585 1106 12-13 0.40072374500397956 1106 14-15 0.4992916426143239 1106 16-17 0.5239502439921466 1106 18-19 0.4357455749610253 1106 20-21 0.4279953147278803 1106 22-23 0.2815596971612919 1106 24-25 0.26944773747163 1106 26-27 0.35291442633452164 1106 28-29 0.5684047989241279 1106 30-31 0.4634197507677058 1106 32-33 0.4795825983003681 1106 34-35 0.654476166143148 1106 36-37 0.6078042184752519 1106 38-39 0.5945918846771221 1106 40-41 0.6119094294315985 1106 42-43 0.5609925936696172 1106 44-45 0.4729307770568738 1106 46-47 0.5703653348632471 1106 48-49 0.5687014364048864 1106 50-51 0.4867866363111375 1106 52-53 0.5104328052534726 1106 54-55 0.3850986109873986 1106 56-57 0.5116434308602891 1106 58-59 0.49704909209253145 1106 60-61 0.5747526065100459 1106 62-63 0.3730333923792486 1106 64-65 0.5152907762215477 1106 66-67 0.3478664633723412 1106 68-69 0.33760073308247485 1106 70-71 0.28287622293999704 1106 72-73 0.25960872838047777 1106 74-75 0.46371946084686044 1106 76-77 0.4963401416262698 1106 78-79 0.5266966588978192 1106 80-81 0.42050418293738545 1106 82-83 0.5327903570072472 1106 84-85 0.5993867640482513 1106 86-87 0.7487892026110252 1106 88-89 0.6599679033840928 1106 90-91 0.7388554523393474 1106 92-93 0.8441663924405738 1106 94-95 0.827420353399198 1106 96-97 0.9297821368166588 1106 98-99 0.9394165233220377 1106 100-101 1.0433134927969085 1107 1 0.05152272900642885 1107 2 0.11994547248858467 1107 3 0.04786433079103247 1107 4 -0.06692839832561503 1107 5 0.04927834530531783 1107 6 -0.008806952577280924 1107 7 0.2028587851252368 1107 8 0.04331295886379394 1107 9 0.19059504005324612 1107 10-11 0.15211040414691723 1107 12-13 0.06049304007962064 1107 14-15 0.14097936160173674 1107 16-17 0.058375497816442135 1107 18-19 0.212656906760877 1107 20-21 0.18107704864487317 1107 22-23 0.24538916267163557 1107 24-25 0.17687403907527255 1107 26-27 0.17441951198463101 1107 28-29 0.2854267330494551 1107 30-31 0.24020227393035043 1107 32-33 0.19711009359859588 1107 34-35 0.1828525052693024 1107 36-37 0.2603528475542305 1107 38-39 0.2503482561225354 1107 40-41 0.24271230945016953 1107 42-43 0.14042577457091454 1107 44-45 0.07828224135142392 1107 46-47 0.06124054118617295 1107 48-49 0.1435761661867616 1107 50-51 0.1124514263428722 1107 52-53 0.26301865137347846 1107 54-55 0.28397313915575495 1107 56-57 0.1388147219097675 1107 58-59 0.2001747125886837 1107 60-61 0.2424981091539351 1107 62-63 0.007097727456844893 1107 64-65 0.14131577222491387 1107 66-67 -0.06423971567472364 1107 68-69 0.07422474525259304 1107 70-71 0.028132195740504073 1107 72-73 -0.017923481415692777 1107 74-75 0.3183876793266798 1107 76-77 0.27246538435163714 1107 78-79 0.42534182623194994 1107 80-81 0.4098105204241591 1107 82-83 0.43787952007694386 1107 84-85 0.2604642559353465 1107 86-87 0.37318067089711704 1107 88-89 0.45469861187232397 1107 90-91 0.5806104776581336 1107 92-93 0.31187539081849636 1107 94-95 0.3082469586450216 1107 96-97 0.31622510411963134 1107 98-99 0.3911227031837008 1107 100-101 0.5300105644114517 1108 1 0.02706652611263749 1108 2 0.1464902399049386 1108 3 0.06233276714286973 1108 4 0.11571998719462329 1108 5 0.1002476861576227 1108 6 0.02809748258692224 1108 7 0.03221734992327896 1108 8 0.08891906600194943 1108 9 0.17132606824529262 1108 10-11 0.1754340683890021 1108 12-13 0.13571962433284313 1108 14-15 0.034043990787154144 1108 16-17 0.1583077495583609 1108 18-19 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-1.0305393099392361 2316 90-91 -0.9095752347792185 2316 92-93 -0.8719934768134685 2316 94-95 -1.493188328497979 2316 96-97 -1.2240726053493916 2316 98-99 -1.237615865212188 2316 100-101 -1.561833333906744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 27.0 5 54.0 6 112.0 7 259.0 8 660.0 9 1229.0 10 2149.0 11 3525.0 12 5169.0 13 6677.0 14 8340.0 15 10546.0 16 12997.0 17 15107.0 18 13147.0 19 10070.0 20 9557.0 21 9853.0 22 10983.0 23 12904.0 24 15585.0 25 19948.0 26 25401.0 27 32916.0 28 41762.0 29 52189.0 30 63917.0 31 76327.0 32 92938.0 33 112462.0 34 140122.0 35 174165.0 36 227370.0 37 310834.0 38 292053.0 39 57193.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.889889166654626 22.459420245643386 15.16418952079256 22.48650106690943 2 15.085154244224974 21.968622904861988 40.74242961883032 22.203793232082713 3 18.30299825690828 26.91243706914406 31.01445583373784 23.770108840209815 4 12.272511863585386 18.237488286518722 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20.549037174040784 28.10730925706506 30.880992977487505 20.462660591406653 26-27 20.508489679205645 28.195935475601587 30.764447091796853 20.53112775339591 28-29 20.47901426039694 28.19171808977631 30.806208930749857 20.523058719076896 30-31 20.843079490374887 28.01369996812903 30.62864485757476 20.514575683921326 32-33 20.74254104136775 28.300832911136965 30.409084862942077 20.547541184553207 34-35 20.797116788838824 28.146607658643386 30.705171212741195 20.351104339776597 36-37 20.83966172917998 27.988956213387624 30.695877364518815 20.47550469291359 38-39 20.689866472364212 28.229531279225313 30.742877754554364 20.337724493856115 40-41 20.682413686806413 28.068565685367858 30.808078375462394 20.440942252363335 42-43 20.654821241481557 28.22448414328513 30.372637148106175 20.74805746712714 44-45 20.5616962745809 28.55223387330926 30.24957886159499 20.63649099051485 46-47 20.78805471354814 28.274545055075624 30.08257664170483 20.854823589671405 48-49 20.92318569634054 28.478089948078267 29.760906320899984 20.837818034681217 50-51 20.943761028471485 28.660353372198948 29.392528600971872 21.00335699835769 52-53 20.82606136766621 28.85040034096422 29.318958258939393 21.004580032430177 54-55 20.863438160623488 28.83249605850566 29.39561255679405 20.908453224076794 56-57 20.87361272749021 28.87199321151997 29.273125802298633 20.981268258691184 58-59 20.781763264143844 28.810663021189338 29.549723243825376 20.857850470841445 60-61 20.87380942748837 28.978342705381543 29.434598785335396 20.713249081794686 62-63 20.802293600866456 28.838721063781055 29.446748452237838 20.912236883114655 64-65 20.60591982369496 28.634065112266782 29.5402678695008 21.21974719453746 66-67 20.62293823331292 28.698397318016607 29.377906794263147 21.300757654407327 68-69 20.597427548021223 28.79569986504405 29.415434980121425 21.191437606813306 70-71 20.729944313825204 28.404052790558083 29.551597819329178 21.31440507628754 72-73 20.802731123291842 28.76604170441133 29.355233634540195 21.07599353775663 74-75 20.656931231921792 28.92928730318048 29.205553002259865 21.208228462637862 76-77 20.563222798121966 28.784854522004704 29.343813934032813 21.308108745840517 78-79 20.515921894823094 28.816088795146644 29.26709698067494 21.400892329355322 80-81 20.57260441487931 28.867354270049116 29.137534501697154 21.42250681337442 82-83 20.694911825526287 28.73719340157811 29.333933793150692 21.233960979744918 84-85 20.69279790727445 28.766520672514993 29.034667188991786 21.506014231218774 86-87 20.403191106772674 28.924957507567296 29.24587170496337 21.42597968069666 88-89 20.55462822580084 28.715855519229443 29.157771704198993 21.571744550770724 90-91 20.561878333284273 28.572678642629114 29.411602572164853 21.453840451921767 92-93 20.530801126079652 28.72894789593371 29.13708352435419 21.603167453632448 94-95 20.18522116131297 28.585224800551025 29.18575259710477 22.04380144103123 96-97 20.300203230549606 28.555377314440072 29.206277000964644 21.93814245404568 98-99 20.178714395194607 28.691088576920937 29.139934966241526 21.990262061642927 100-101 20.194876504360558 28.58030855714108 29.032125135397802 22.192689803100556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 334.0 1 240.0 2 213.0 3 292.0 4 255.5 5 239.5 6 414.0 7 915.0 8 1529.5 9 1854.0 10 1687.5 11 1433.5 12 1468.0 13 1609.0 14 1800.5 15 2077.0 16 2357.5 17 2568.0 18 2796.0 19 3100.5 20 3566.5 21 4155.0 22 4844.0 23 6080.0 24 7666.5 25 9544.0 26 12413.5 27 15990.5 28 19872.0 29 24992.0 30 30848.0 31 35995.5 32 42501.5 33 49660.5 34 54641.0 35 59204.0 36 66404.5 37 73101.5 38 74626.0 39 73789.0 40 72624.0 41 70787.0 42 68841.5 43 69741.5 44 72262.0 45 74055.5 46 73038.0 47 70616.0 48 70618.0 49 69709.5 50 66466.0 51 62049.0 52 57282.0 53 52682.5 54 47663.0 55 41082.0 56 33673.0 57 26729.0 58 21090.0 59 16339.0 60 12052.0 61 8399.0 62 5586.5 63 3522.0 64 2013.0 65 1165.5 66 681.0 67 377.5 68 210.0 69 120.0 70 65.0 71 37.5 72 26.5 73 16.0 74 9.5 75 8.0 76 7.0 77 5.0 78 2.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0046559718458199 2 0.007224783898686052 3 0.002622328970634197 4 0.1710400691866713 5 0.001926609039649614 6 4.2813534214435864E-4 7 0.0 8 8.027537665206726E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.478599654922914E-4 24-25 3.21101506608269E-4 26-27 0.0018463336629975468 28-29 8.027537665206726E-5 30-31 0.005164382564616327 32-33 0.019346365773148206 34-35 0.012549717216606514 36-37 3.21101506608269E-4 38-39 1.8730921218815692E-4 40-41 0.001284406026433076 42-43 0.010462557423652765 44-45 0.006448788591049402 46-47 0.009766837492668183 48-49 0.009579528280480025 50-51 0.1877908644480693 52-53 0.11219821810070599 54-55 0.01629590146036965 56-57 0.005806585577832864 58-59 0.016964862932470213 60-61 0.12263401706547473 62-63 0.23681236112359838 64-65 0.24221756981817089 66-67 0.25278716107735977 68-69 0.24759602005385942 70-71 0.23392244756412395 72-73 0.1585171104289488 74-75 0.26563122134169054 76-77 0.20620068416027673 78-79 0.004682730304703923 80-81 2.6758458884022415E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.351691776804483E-5 90-91 0.005512242530108617 92-93 0.01693810447358619 94-95 0.0024082612995620176 96-97 2.6758458884022415E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1868568.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.88527358891895 #Duplication Level Percentage of deduplicated Percentage of total 1 72.79504849913778 37.76991007296387 2 15.265554731893014 15.841149675017704 3 5.1024849701852055 7.942314859842191 4 2.3405792521299564 4.857663794132404 5 1.2097743616053425 3.138473686637648 6 0.7316119262582604 2.27759309728955 7 0.5022561606477824 1.8241788814851154 8 0.3382378929928637 1.403965249285938 9 0.25762129233462777 1.2030076111601726 >10 1.2760015402599696 12.130122269538699 >50 0.12099258147723367 4.339561383268627 >100 0.05673157429937488 5.3174656761626995 >500 0.0022771589709471123 0.7717946609080778 >1k 6.210433557128489E-4 0.5586931916656275 >5k 2.0701445190428295E-4 0.6241058906417627 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6151 0.3291825611912438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5470 0.29273754019120524 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3433 0.18372357869769793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.351691776804484E-5 0.0 22-23 0.0 0.0 0.0 5.351691776804484E-5 0.0 24-25 0.0 0.0 0.0 3.2110150660826897E-4 0.0 26-27 0.0 0.0 0.0 5.351691776804483E-4 0.0 28-29 0.0 0.0 0.0 0.0012308891086650312 0.0 30-31 0.0 0.0 0.0 0.003906734997067273 0.0 32-33 0.0 0.0 0.0 0.011532895779013662 0.0 34-35 0.0 0.0 0.0 0.023815028406779952 0.0 36-37 0.0 0.0 0.0 0.04321491109769621 0.0 38-39 0.0 0.0 0.0 0.0744955495331184 0.0 40-41 0.0 0.0 0.0 0.11393751792816745 0.0 42-43 0.0 0.0 0.0 0.15126556807137873 0.0 44-45 0.0 0.0 0.0 0.19720984197524521 0.0 46-47 0.0 0.0 0.0 0.2557841084723703 0.0 48-49 0.0 0.0 0.0 0.32353652636671504 0.0 50-51 0.0 0.0 0.0 0.4000657187750192 0.0 52-53 0.0 0.0 0.0 0.47346417149389264 0.0 54-55 0.0 0.0 0.0 0.5536860312281919 0.0 56-57 0.0 0.0 0.0 0.6508459954360772 0.0 58-59 0.0 0.0 0.0 0.7665495716505901 0.0 60-61 0.0 0.0 0.0 0.8914312992623228 0.0 62-63 0.0 0.0 0.0 1.0271769611809685 0.0 64-65 0.0 0.0 0.0 1.1620128354975576 0.0 66-67 0.0 0.0 0.0 1.3170781047304674 0.0 68-69 0.0 0.0 0.0 1.4948077886381443 0.0 70-71 0.0 0.0 0.0 1.6972622885546578 0.0 72-73 0.0 0.0 0.0 1.9053360648368163 0.0 74-75 0.0 0.0 0.0 2.1109480629016444 0.0 76-77 0.0 0.0 0.0 2.3478942163196628 0.0 78-79 0.0 0.0 0.0 2.622462762928617 0.0 80-81 0.0 0.0 0.0 2.931496204580192 0.0 82-83 0.0 0.0 0.0 3.2446236904410224 0.0 84-85 0.0 0.0 0.0 3.54482684066087 0.0 86-87 0.0 0.0 0.0 3.8669184102478473 0.0 88-89 0.0 0.0 0.0 4.237201964284949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2715 0.0 61.07612 1 GTATCAA 5480 0.0 43.698433 1 ATCAACG 6995 0.0 33.892643 3 CAACGCA 7085 0.0 33.8128 5 TCAACGC 7165 0.0 33.21933 4 TATCAAC 7170 0.0 33.00092 2 AACGCAG 7695 0.0 31.748869 6 GTGGTAT 1300 0.0 28.508026 1 CGCAGAG 8500 0.0 27.960726 8 ACGCAGA 8535 0.0 27.957228 7 ACATGGG 5365 0.0 25.504414 3 GTACATG 5580 0.0 25.033926 1 TGGTATC 1405 0.0 25.02484 2 GCAGAGT 9425 0.0 24.712585 9 TACATGG 5640 0.0 24.683365 2 CTAGACG 105 0.002194507 22.622992 4 TAACGCA 295 5.456968E-12 22.546303 4 GAGTACT 6920 0.0 21.25072 12-13 AGAGTAC 9005 0.0 20.88707 10-11 CAGAGTA 9390 0.0 20.838978 10-11 >>END_MODULE