FastQCFastQC Report
Sat 18 Jun 2016
SRR1931022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663192
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1196504.492729025920775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20600.054.516641
GTATCAA41700.049.4358631
ATCAACG50800.041.3793533
TCAACGC53950.039.429394
TATCAAC53300.038.3804552
CAACGCA54150.038.3441665
AACGCAG58350.035.5939986
ACGCAGA64050.031.3441187
CGCAGAG65750.030.1337498
GTACATG48650.026.5625531
GCAGAGT69900.025.8651529
TACATGG48800.025.6350652
ACATGGG48200.025.518443
GTACTAG7350.023.7215861
GAGTACT44850.021.52768712-13
GAGTACA35150.021.1541121
AGAGTAC62850.021.12214510-11
GTGGTAT8750.021.0982571
CATGGGG34200.020.7822254
CGATAGT1504.6110054E-420.5121941
Produced by FastQC (version 0.11.5)