##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931022_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2663192 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.607820239772423 28.0 2.0 31.0 2.0 33.0 2 19.386088197921893 27.0 2.0 31.0 2.0 33.0 3 19.99259798016816 27.0 2.0 31.0 2.0 33.0 4 21.51688687860282 30.0 2.0 35.0 2.0 37.0 5 20.87737159018201 28.0 2.0 35.0 2.0 37.0 6 21.792741942751405 30.0 2.0 35.0 2.0 37.0 7 21.946773270571555 29.0 2.0 35.0 2.0 37.0 8 22.338128831867923 30.0 2.0 35.0 2.0 37.0 9 23.554958110417875 32.0 2.0 37.0 2.0 39.0 10-11 23.843688889122525 32.0 2.0 37.0 2.0 39.0 12-13 23.70729598166411 32.0 2.0 37.0 2.0 39.0 14-15 23.637140506580074 32.0 2.0 38.0 2.0 40.0 16-17 22.53526107017444 30.0 2.0 37.5 2.0 40.0 18-19 21.545336385810714 27.0 2.0 37.0 2.0 39.5 20-21 21.217308966082804 27.0 2.0 37.0 2.0 39.5 22-23 20.849389003872044 27.0 2.0 37.0 2.0 39.0 24-25 20.666331792826053 26.0 2.0 37.0 2.0 39.0 26-27 20.367025171298202 25.0 2.0 36.0 2.0 39.0 28-29 20.14194245101367 24.0 2.0 36.0 2.0 39.0 30-31 20.083098589962724 24.5 2.0 36.0 2.0 39.0 32-33 19.930819670530703 24.5 2.0 36.0 2.0 39.0 34-35 19.58029668908588 24.0 2.0 35.5 2.0 39.0 36-37 19.14352082012863 21.5 2.0 35.0 2.0 39.0 38-39 18.975551143139512 20.0 2.0 35.0 2.0 38.0 40-41 18.80677172355579 20.0 2.0 35.0 2.0 38.0 42-43 18.639286052226048 19.0 2.0 35.0 2.0 38.0 44-45 18.23025320742928 17.0 2.0 34.0 2.0 38.0 46-47 17.879872160925686 15.0 2.0 34.0 2.0 38.0 48-49 17.586351641188468 14.0 2.0 33.5 2.0 38.0 50-51 15.492314298030333 7.0 2.0 31.5 2.0 37.5 52-53 17.32216884850961 14.5 2.0 33.5 2.0 38.0 54-55 18.002881316855863 15.0 2.0 35.0 2.0 38.5 56-57 17.933100016821918 14.0 2.0 35.0 2.0 39.0 58-59 17.64390851279217 11.0 2.0 34.0 2.0 38.5 60-61 17.336040736079113 8.0 2.0 34.0 2.0 38.0 62-63 16.89345417078453 7.5 2.0 34.0 2.0 38.0 64-65 16.5225453140442 4.5 2.0 33.5 2.0 38.0 66-67 16.138404966671573 2.0 2.0 33.0 2.0 37.5 68-69 15.688359870411144 2.0 2.0 32.0 2.0 37.0 70-71 15.323814993436446 2.0 2.0 31.5 2.0 36.0 72-73 14.925942440499972 2.0 2.0 31.0 2.0 36.0 74-75 14.468853541164137 2.0 2.0 31.0 2.0 35.5 76-77 14.045976782747921 2.0 2.0 30.5 2.0 35.0 78-79 13.657400217483382 2.0 2.0 30.0 2.0 34.5 80-81 13.220240223010583 2.0 2.0 29.0 2.0 34.0 82-83 12.752047918437725 2.0 2.0 29.0 2.0 34.0 84-85 12.343108570467319 2.0 2.0 29.0 2.0 34.0 86-87 11.858772668286777 2.0 2.0 27.5 2.0 34.0 88-89 11.434898610389336 2.0 2.0 26.0 2.0 34.0 90-91 10.951091209345778 2.0 2.0 26.0 2.0 33.0 92-93 10.408529313695745 2.0 2.0 25.0 2.0 33.0 94-95 9.850894715814707 2.0 2.0 22.5 2.0 32.0 96-97 9.231959806127385 2.0 2.0 18.0 2.0 32.0 98-99 8.585144818698765 2.0 2.0 2.0 2.0 32.0 100-101 7.731724937593684 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.975655996801599 1101 2 9.491605540229695 1101 3 8.879077293980597 1101 4 11.098056438613394 1101 5 11.677809982961627 1101 6 11.13066378634247 1101 7 10.610445384600784 1101 8 10.18597648871928 1101 9 10.011994308834325 1101 10-11 9.416264877202575 1101 12-13 9.475038009370184 1101 14-15 10.173016907847707 1101 16-17 10.887755123993408 1101 18-19 11.834637855045312 1101 20-21 11.981424583183568 1101 22-23 12.26288833082204 1101 24-25 11.912855290323701 1101 26-27 11.872608323877756 1101 28-29 11.927380132880216 1101 30-31 11.559139143432656 1101 32-33 11.536689800137527 1101 34-35 11.551792714757031 1101 36-37 11.756596702372171 1101 38-39 11.602983347674144 1101 40-41 11.255363787883269 1101 42-43 10.958397417297672 1101 44-45 10.881674185906487 1101 46-47 10.604803412751739 1101 48-49 10.812729780804112 1101 50-51 12.315906297410306 1101 52-53 11.60115935877965 1101 54-55 11.47321900808615 1101 56-57 11.250424815091133 1101 58-59 10.82653805560852 1101 60-61 10.688313834541525 1101 62-63 10.753512984742386 1101 64-65 10.50402350696583 1101 66-67 10.190275615680363 1101 68-69 10.264680791546867 1101 70-71 10.155447789667798 1101 72-73 10.040984112680272 1101 74-75 9.53255920993026 1101 76-77 9.324420977067346 1101 78-79 9.109632974331957 1101 80-81 8.888316939559212 1101 82-83 8.566397566523436 1101 84-85 8.075573059899057 1101 86-87 7.84455687187859 1101 88-89 7.690694123840332 1101 90-91 7.100173880745075 1101 92-93 6.94603525464907 1101 94-95 6.558617357644904 1101 96-97 6.283386111975865 1101 98-99 5.814514473417242 1101 100-101 5.054761916139659 1102 1 7.8763370175880425 1102 2 9.005875389615873 1102 3 8.491051383974526 1102 4 10.40602238728873 1102 5 11.179705068038572 1102 6 10.2853291510982 1102 7 10.238814963712993 1102 8 9.547918049962359 1102 9 9.51600533208611 1102 10-11 8.603405348903703 1102 12-13 8.909497498520413 1102 14-15 9.333829207824419 1102 16-17 10.090245528011906 1102 18-19 10.948006401227008 1102 20-21 11.207236674862145 1102 22-23 11.219469867274785 1102 24-25 10.96317198910062 1102 26-27 11.146175118528888 1102 28-29 10.751307283315988 1102 30-31 11.051960419061889 1102 32-33 10.831047032020727 1102 34-35 10.499922554122069 1102 36-37 10.584006783203264 1102 38-39 10.466934415301989 1102 40-41 10.315882530150557 1102 42-43 10.09979247840607 1102 44-45 9.974955586423295 1102 46-47 10.023007158009392 1102 48-49 9.497542592109966 1102 50-51 11.287449061874758 1102 52-53 10.727095630391297 1102 54-55 10.39891984573002 1102 56-57 10.447129524227034 1102 58-59 10.454809121113435 1102 60-61 10.139495170343238 1102 62-63 10.041809311390395 1102 64-65 9.764439056051142 1102 66-67 9.590142205000888 1102 68-69 9.441406855522361 1102 70-71 9.154640634369835 1102 72-73 9.115985924588761 1102 74-75 8.756718612793703 1102 76-77 8.516771470362999 1102 78-79 8.104669201088166 1102 80-81 7.733134324863977 1102 82-83 7.850121106952679 1102 84-85 7.459409252528609 1102 86-87 7.082713553169876 1102 88-89 6.777076789782614 1102 90-91 6.560629263283182 1102 92-93 6.3630315840363405 1102 94-95 6.102397061530548 1102 96-97 5.867637716727575 1102 98-99 5.309029170135149 1102 100-101 4.536910480080913 1103 1 7.933183855550325 1103 2 9.28524759542022 1103 3 8.619035155910215 1103 4 10.472548480563049 1103 5 11.100045280163432 1103 6 10.486121499180872 1103 7 10.176478213827767 1103 8 9.782487431818307 1103 9 9.770363218682434 1103 10-11 9.189172669016568 1103 12-13 9.017575969451872 1103 14-15 9.469974928331307 1103 16-17 10.363895552638716 1103 18-19 10.985762726193915 1103 20-21 11.335604962106572 1103 22-23 11.458489207677463 1103 24-25 11.390266744370312 1103 26-27 11.096008859708924 1103 28-29 10.978335122753649 1103 30-31 10.812337805410834 1103 32-33 10.92467960894329 1103 34-35 10.908997185054233 1103 36-37 11.067209641807494 1103 38-39 10.812747189149874 1103 40-41 10.50760460713775 1103 42-43 10.291434741130661 1103 44-45 10.36580610002041 1103 46-47 10.033258624474588 1103 48-49 9.913058846918322 1103 50-51 11.520640150312863 1103 52-53 10.669931555111262 1103 54-55 10.460517025236058 1103 56-57 10.039986593458455 1103 58-59 10.044586167701564 1103 60-61 10.024674483600705 1103 62-63 9.891763790795252 1103 64-65 9.59541382917249 1103 66-67 9.515196124898015 1103 68-69 9.364985534834505 1103 70-71 9.030636501857542 1103 72-73 8.769828055041762 1103 74-75 8.92061413662042 1103 76-77 8.70469575351113 1103 78-79 8.314878517822363 1103 80-81 8.081717266508472 1103 82-83 7.554590691729077 1103 84-85 7.468306871305607 1103 86-87 7.311082634378176 1103 88-89 6.774765658464892 1103 90-91 6.466858996310741 1103 92-93 6.013263477202793 1103 94-95 5.782939987953219 1103 96-97 5.492705273302375 1103 98-99 5.025589013838486 1103 100-101 4.636251796512008 1104 1 7.956386449844757 1104 2 9.33273494510166 1104 3 8.80296638927259 1104 4 10.660099771745124 1104 5 11.345480139006483 1104 6 10.664591313755341 1104 7 10.434608180009231 1104 8 9.940533162965952 1104 9 9.728968749272767 1104 10-11 9.141863470205418 1104 12-13 9.080446154708685 1104 14-15 9.472713872432909 1104 16-17 10.446120916100632 1104 18-19 11.362522091567083 1104 20-21 11.572094518287962 1104 22-23 11.625322376115793 1104 24-25 11.477192920818805 1104 26-27 11.68230116451522 1104 28-29 11.442143783496952 1104 30-31 11.30595590698535 1104 32-33 11.07322911540312 1104 34-35 11.030670614223492 1104 36-37 10.970623349904379 1104 38-39 10.625914526844792 1104 40-41 10.436496500690698 1104 42-43 10.015190225747222 1104 44-45 10.083014791212047 1104 46-47 10.146007438174568 1104 48-49 10.169087757570939 1104 50-51 11.432633218697491 1104 52-53 10.703847303206334 1104 54-55 10.542112935679395 1104 56-57 10.20328968332161 1104 58-59 10.311120155151873 1104 60-61 9.912417342201135 1104 62-63 10.001871874345802 1104 64-65 9.757164777067675 1104 66-67 9.676014944057005 1104 68-69 9.439430505496853 1104 70-71 9.01881387183653 1104 72-73 8.834822794871195 1104 74-75 8.434393413516682 1104 76-77 8.357217401754143 1104 78-79 8.10911586941184 1104 80-81 7.815368459556931 1104 82-83 7.54123620523942 1104 84-85 7.289037687549914 1104 86-87 7.115233971433595 1104 88-89 6.851572383449353 1104 90-91 6.461220430885188 1104 92-93 6.098400311933817 1104 94-95 5.67440332763732 1104 96-97 5.217797994102002 1104 98-99 4.728332537240922 1104 100-101 4.19426792288143 1105 1 8.042165114923623 1105 2 9.043366213124962 1105 3 8.492652681638297 1105 4 10.761615188601507 1105 5 10.610026623075928 1105 6 10.507165420908624 1105 7 10.153148102720635 1105 8 9.621246039339223 1105 9 9.541208834266875 1105 10-11 8.897232809282851 1105 12-13 8.5751135919263 1105 14-15 9.531855992382003 1105 16-17 10.344101138626407 1105 18-19 10.7668039030623 1105 20-21 11.133117483041097 1105 22-23 11.268396757631912 1105 24-25 11.348503908174763 1105 26-27 11.549787417185883 1105 28-29 11.29201068833391 1105 30-31 11.134490809440607 1105 32-33 11.073896926221185 1105 34-35 10.917524548119562 1105 36-37 10.960271114722893 1105 38-39 10.747991978889669 1105 40-41 10.639886341093252 1105 42-43 10.239998463637313 1105 44-45 10.226330845524213 1105 46-47 10.075699918297854 1105 48-49 10.00740165200546 1105 50-51 11.655555358027113 1105 52-53 10.767773260265631 1105 54-55 10.50403020652805 1105 56-57 10.45776413255281 1105 58-59 10.544551558605338 1105 60-61 10.288028821075194 1105 62-63 10.223352149409138 1105 64-65 10.034367560391086 1105 66-67 9.622426044660603 1105 68-69 9.309305466093681 1105 70-71 9.256276073977725 1105 72-73 8.956921845692532 1105 74-75 8.72808890579366 1105 76-77 8.392116938256075 1105 78-79 8.089598164252651 1105 80-81 7.86644431212485 1105 82-83 7.748487556622324 1105 84-85 7.362602536416851 1105 86-87 7.040638802554513 1105 88-89 6.710550469447513 1105 90-91 6.512937825488997 1105 92-93 6.180947333053917 1105 94-95 5.709020328793146 1105 96-97 5.282060191573194 1105 98-99 4.612376295194526 1105 100-101 4.098216996469045 1106 1 7.950094347083645 1106 2 9.030536199819387 1106 3 8.636552170424054 1106 4 10.40869337318614 1106 5 11.02011212254434 1106 6 10.233667709595064 1106 7 9.491693529485854 1106 8 9.040511113156523 1106 9 8.760358677974097 1106 10-11 8.58854439532028 1106 12-13 8.473286780871849 1106 14-15 8.94597008928989 1106 16-17 9.85721190595708 1106 18-19 10.868820429988592 1106 20-21 11.092556484295251 1106 22-23 11.117799598276946 1106 24-25 10.85525091760326 1106 26-27 10.886806204150233 1106 28-29 10.98013721226803 1106 30-31 10.537589926434183 1106 32-33 10.925020260636789 1106 34-35 10.534364696581783 1106 36-37 10.766361758165282 1106 38-39 10.421270052700603 1106 40-41 10.603798626150898 1106 42-43 10.073734784040685 1106 44-45 9.650829976523656 1106 46-47 9.456957836388984 1106 48-49 9.712382011956311 1106 50-51 11.510786550372265 1106 52-53 10.881248498524574 1106 54-55 10.183372821933236 1106 56-57 10.07018861226658 1106 58-59 9.693209859947995 1106 60-61 9.528412518242828 1106 62-63 9.73163120999726 1106 64-65 9.524654808046694 1106 66-67 8.993327258714856 1106 68-69 8.83509472868464 1106 70-71 8.558910132433136 1106 72-73 8.340159684459506 1106 74-75 8.030963928871513 1106 76-77 7.809874789501007 1106 78-79 7.26678801381688 1106 80-81 6.949762048012348 1106 82-83 6.72056685531023 1106 84-85 6.631670254797012 1106 86-87 6.394087111345742 1106 88-89 6.028811258741934 1106 90-91 5.5703469305775855 1106 92-93 5.436212585127739 1106 94-95 5.168367336948384 1106 96-97 4.570125598984845 1106 98-99 4.284441588012697 1106 100-101 3.5195877254745653 1107 1 7.258982546794819 1107 2 8.197789607333302 1107 3 8.056440586286417 1107 4 9.917927414806208 1107 5 10.460786138351573 1107 6 9.716473038818393 1107 7 9.76133493778919 1107 8 8.200087707428203 1107 9 5.798715803601798 1107 10-11 7.8536616651740765 1107 12-13 7.777386448500231 1107 14-15 8.35514210491543 1107 16-17 9.055399995086553 1107 18-19 10.400743141840252 1107 20-21 10.724538089515463 1107 22-23 10.012144268583725 1107 24-25 10.275624577230918 1107 26-27 10.283746568349333 1107 28-29 10.356609311534683 1107 30-31 10.304474310606903 1107 32-33 10.603964628331898 1107 34-35 10.647376058616235 1107 36-37 10.842162222420452 1107 38-39 10.313071343969035 1107 40-41 9.919584241758894 1107 42-43 9.74907851142704 1107 44-45 9.257067034862551 1107 46-47 9.540156310173604 1107 48-49 9.581100235381463 1107 50-51 10.867744582581926 1107 52-53 9.919758809563007 1107 54-55 9.78255896570197 1107 56-57 9.382227920457456 1107 58-59 9.607850187495366 1107 60-61 9.475515309099322 1107 62-63 9.555009997733656 1107 64-65 9.521910940945219 1107 66-67 9.587878055811537 1107 68-69 9.211518585511103 1107 70-71 8.819537722723249 1107 72-73 8.488585383270047 1107 74-75 8.537601336811468 1107 76-77 8.431756808541108 1107 78-79 7.855954766630761 1107 80-81 7.357227722263099 1107 82-83 7.028814119744586 1107 84-85 6.871691118086083 1107 86-87 6.403010083205361 1107 88-89 6.229396120013591 1107 90-91 6.131982599910117 1107 92-93 5.533373799458877 1107 94-95 5.1345622301414995 1107 96-97 4.804785912686011 1107 98-99 4.544423857349615 1107 100-101 4.12209194668808 1108 1 6.698337066616428 1108 2 7.761919736856214 1108 3 7.805275104760003 1108 4 9.084444675166356 1108 5 10.757911471145693 1108 6 9.373658583477397 1108 7 8.710744614375088 1108 8 5.788412671761659 1108 9 7.053107562664472 1108 10-11 6.133531692920965 1108 12-13 5.133271708950247 1108 14-15 7.227791202299134 1108 16-17 8.296091869854862 1108 18-19 7.8936171350327164 1108 20-21 8.109459286113943 1108 22-23 8.83949348616649 1108 24-25 8.38279607994382 1108 26-27 6.9494050232859195 1108 28-29 8.935290828963545 1108 30-31 9.238285070107683 1108 32-33 9.6753808428479 1108 34-35 9.158856192070889 1108 36-37 8.98168973901803 1108 38-39 8.522549657587476 1108 40-41 8.743904330728665 1108 42-43 7.934865253583208 1108 44-45 7.398238020831837 1108 46-47 8.352234630532177 1108 48-49 9.035833494573321 1108 50-51 10.459074523381082 1108 52-53 9.821914529128495 1108 54-55 9.28435689648079 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6.166481157111111 1109 18-19 5.364200264731341 1109 20-21 7.66693434920732 1109 22-23 8.703176180999286 1109 24-25 8.10263366178717 1109 26-27 8.481352305263243 1109 28-29 8.924896556551488 1109 30-31 6.528322102693689 1109 32-33 5.530601013144381 1109 34-35 7.069867822617432 1109 36-37 5.249599578914413 1109 38-39 5.710535654640893 1109 40-41 6.482622077438236 1109 42-43 5.547611006185949 1109 44-45 6.068091626390288 1109 46-47 5.247249252172207 1109 48-49 6.099364186664282 1109 50-51 7.7530362534081885 1109 52-53 7.989648330710391 1109 54-55 8.126464326588465 1109 56-57 7.27780876635277 1109 58-59 7.154762103904137 1109 60-61 7.128119215791276 1109 62-63 7.553261303846597 1109 64-65 7.655645259270731 1109 66-67 7.4141766814812335 1109 68-69 6.791743869195505 1109 70-71 6.445638351654063 1109 72-73 6.047112553746905 1109 74-75 5.225275623856826 1109 76-77 5.045962658596013 1109 78-79 5.607485497721237 1109 80-81 5.572242197654932 1109 82-83 5.573386679725951 1109 84-85 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56-57 -15.72323945167658 2313 58-59 -15.456523710211865 2313 60-61 -15.144766877849534 2313 62-63 -14.70442562836864 2313 64-65 -14.33928835358741 2313 66-67 -13.954097252830437 2313 68-69 -13.506823566164384 2313 70-71 -13.14712894394342 2313 72-73 -12.770497509146177 2313 74-75 -12.328183495640031 2313 76-77 -11.888349011564712 2313 78-79 -11.518824774815448 2313 80-81 -11.09197862694325 2313 82-83 -10.63538341111961 2313 84-85 -10.220372373977412 2313 86-87 -9.741081627553013 2313 88-89 -9.313284656000517 2313 90-91 -8.842796941536093 2313 92-93 -8.316185283433539 2313 94-95 -7.748506552927283 2313 96-97 -7.141774933874835 2313 98-99 -6.513071733479309 2313 100-101 -5.6754389175094495 2314 1 -18.36500334385774 2314 2 -17.161293285082923 2314 3 -17.744264881085716 2314 4 -19.257381227169102 2314 5 -18.614346136780703 2314 6 -19.528676872998187 2314 7 -19.686636843515924 2314 8 -20.08950664883282 2314 9 -21.302518764299435 2314 10-11 -21.581082602903525 2314 12-13 -21.470585332804884 2314 14-15 -21.378896695790036 2314 16-17 -20.2996264599399 2314 18-19 -19.306135165038054 2314 20-21 -18.97405097574027 2314 22-23 -18.608843385737263 2314 24-25 -18.43178283017649 2314 26-27 -18.12189717062774 2314 28-29 -17.904942716684012 2314 30-31 -17.85654861708269 2314 32-33 -17.709615618581683 2314 34-35 -17.34627473503456 2314 36-37 -16.913922433664204 2314 38-39 -16.74089690999922 2314 40-41 -16.58359036141571 2314 42-43 -16.42129185894333 2314 44-45 -16.015067003938153 2314 46-47 -15.674658504641211 2314 48-49 -15.37180981752859 2314 50-51 -13.365412383908373 2314 52-53 -15.13491491177738 2314 54-55 -15.781991298848293 2314 56-57 -15.72323945167658 2314 58-59 -15.456523710211865 2314 60-61 -15.144766877849534 2314 62-63 -14.70442562836864 2314 64-65 -14.33928835358741 2314 66-67 -13.954097252830437 2314 68-69 -13.506823566164384 2314 70-71 -13.14712894394342 2314 72-73 -12.770497509146177 2314 74-75 -12.328183495640031 2314 76-77 -11.888349011564712 2314 78-79 -11.518824774815448 2314 80-81 -11.09197862694325 2314 82-83 -10.63538341111961 2314 84-85 -10.220372373977412 2314 86-87 -9.741081627553013 2314 88-89 -9.313284656000517 2314 90-91 -8.842796941536093 2314 92-93 -8.316185283433539 2314 94-95 -7.748506552927283 2314 96-97 -7.141774933874835 2314 98-99 -6.513071733479309 2314 100-101 -5.6754389175094495 2315 1 -18.36500334385774 2315 2 -17.161293285082923 2315 3 -17.744264881085716 2315 4 -19.257381227169102 2315 5 -18.614346136780703 2315 6 -19.528676872998187 2315 7 -19.686636843515924 2315 8 -20.08950664883282 2315 9 -21.302518764299435 2315 10-11 -21.581082602903525 2315 12-13 -21.470585332804884 2315 14-15 -21.378896695790036 2315 16-17 -20.2996264599399 2315 18-19 -19.306135165038054 2315 20-21 -18.97405097574027 2315 22-23 -18.608843385737263 2315 24-25 -18.43178283017649 2315 26-27 -18.12189717062774 2315 28-29 -17.904942716684012 2315 30-31 -17.85654861708269 2315 32-33 -17.709615618581683 2315 34-35 -17.34627473503456 2315 36-37 -16.913922433664204 2315 38-39 -16.74089690999922 2315 40-41 -16.58359036141571 2315 42-43 -16.42129185894333 2315 44-45 -16.015067003938153 2315 46-47 -15.674658504641211 2315 48-49 -15.37180981752859 2315 50-51 -13.365412383908373 2315 52-53 -15.13491491177738 2315 54-55 -15.781991298848293 2315 56-57 -15.72323945167658 2315 58-59 -15.456523710211865 2315 60-61 -15.144766877849534 2315 62-63 -14.70442562836864 2315 64-65 -14.33928835358741 2315 66-67 -13.954097252830437 2315 68-69 -13.506823566164384 2315 70-71 -13.14712894394342 2315 72-73 -12.770497509146177 2315 74-75 -12.328183495640031 2315 76-77 -11.888349011564712 2315 78-79 -11.518824774815448 2315 80-81 -11.09197862694325 2315 82-83 -10.63538341111961 2315 84-85 -10.220372373977412 2315 86-87 -9.741081627553013 2315 88-89 -9.313284656000517 2315 90-91 -8.842796941536093 2315 92-93 -8.316185283433539 2315 94-95 -7.748506552927283 2315 96-97 -7.141774933874835 2315 98-99 -6.513071733479309 2315 100-101 -5.6754389175094495 2316 1 -18.36500334385774 2316 2 -17.161293285082923 2316 3 -17.744264881085716 2316 4 -19.257381227169102 2316 5 -18.614346136780703 2316 6 -19.528676872998187 2316 7 -19.686636843515924 2316 8 -20.08950664883282 2316 9 -21.302518764299435 2316 10-11 -21.581082602903525 2316 12-13 -21.470585332804884 2316 14-15 -21.378896695790036 2316 16-17 -20.2996264599399 2316 18-19 -19.306135165038054 2316 20-21 -18.97405097574027 2316 22-23 -18.608843385737263 2316 24-25 -18.43178283017649 2316 26-27 -18.12189717062774 2316 28-29 -17.904942716684012 2316 30-31 -17.85654861708269 2316 32-33 -17.709615618581683 2316 34-35 -17.34627473503456 2316 36-37 -16.913922433664204 2316 38-39 -16.74089690999922 2316 40-41 -16.58359036141571 2316 42-43 -16.42129185894333 2316 44-45 -16.015067003938153 2316 46-47 -15.674658504641211 2316 48-49 -15.37180981752859 2316 50-51 -13.365412383908373 2316 52-53 -15.13491491177738 2316 54-55 -15.781991298848293 2316 56-57 -15.72323945167658 2316 58-59 -15.456523710211865 2316 60-61 -15.144766877849534 2316 62-63 -14.70442562836864 2316 64-65 -14.33928835358741 2316 66-67 -13.954097252830437 2316 68-69 -13.506823566164384 2316 70-71 -13.14712894394342 2316 72-73 -12.770497509146177 2316 74-75 -12.328183495640031 2316 76-77 -11.888349011564712 2316 78-79 -11.518824774815448 2316 80-81 -11.09197862694325 2316 82-83 -10.63538341111961 2316 84-85 -10.220372373977412 2316 86-87 -9.741081627553013 2316 88-89 -9.313284656000517 2316 90-91 -8.842796941536093 2316 92-93 -8.316185283433539 2316 94-95 -7.748506552927283 2316 96-97 -7.141774933874835 2316 98-99 -6.513071733479309 2316 100-101 -5.6754389175094495 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 720992.0 3 30431.0 4 100333.0 5 56409.0 6 35873.0 7 35168.0 8 33386.0 9 33903.0 10 36600.0 11 38177.0 12 38357.0 13 39297.0 14 39808.0 15 40592.0 16 41962.0 17 42066.0 18 41490.0 19 42789.0 20 44420.0 21 46704.0 22 49245.0 23 52822.0 24 55905.0 25 59655.0 26 63004.0 27 67619.0 28 72227.0 29 76877.0 30 82580.0 31 89004.0 32 94918.0 33 98589.0 34 97812.0 35 86186.0 36 56435.0 37 19696.0 38 1848.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.745449438338284 20.94059873453584 14.053190175581337 23.26076165154454 2 14.82872784414263 20.911674105940737 41.90077140588683 22.358826644029804 3 18.584704409710728 26.67051266731572 30.146311267475962 24.598471655497594 4 12.07311009507004 17.444853127708743 40.09818367675011 30.383853100471104 5 12.490457839168242 38.24855979782084 35.93014327362248 13.330839089388446 6 29.06745867056632 39.13768965661218 18.441657875845944 13.353193796975555 7 25.915226358677863 33.09437878099448 23.489933551719144 17.500461308608514 8 24.36049576506137 36.81812635705769 20.850059287727557 17.971318590153384 9 25.47438854477604 16.750896672975205 21.68107246568532 36.09364231656343 10-11 23.95525761945592 27.325189581639815 29.227642729084774 19.491910069819493 12-13 24.650529365875403 24.849882982280175 29.430937256213085 21.068650395631337 14-15 21.89626325903467 26.507235824689385 27.078738763455423 24.517762152820524 16-17 21.109506893714165 29.409948601658076 27.989485779090206 21.491058725537552 18-19 20.8079205281041 28.41372942518663 30.209762857449924 20.56858718925935 20-21 21.654182287100042 27.746171248616136 30.036813561900267 20.56283290238356 22-23 21.456559784961765 27.681253370551893 29.799791531613163 21.062395312873175 24-25 21.24452755768831 27.807287124649662 30.08626885269007 20.861916464971962 26-27 21.18067769563504 27.997461806182315 29.827503777294375 20.99435672088827 28-29 21.015219936444222 28.115246798136912 29.979955095359006 20.889578170059856 30-31 21.36998008340108 27.809949144793002 29.90093195534812 20.9191388164578 32-33 21.264569744992276 28.079958432243117 29.631696543064486 21.02377527970012 34-35 21.356709056956536 28.077292036454697 29.858343554405703 20.707655352183064 36-37 21.415079273009514 27.976138764311777 29.64511952206779 20.963662440610918 38-39 21.4441183316101 27.983773881046513 29.788074730431298 20.78403305691209 40-41 21.53664048208329 27.935780620071622 29.72029459533182 20.80728430251327 42-43 21.3943358098279 27.882032578739214 29.499754173356646 21.223877438076244 44-45 21.384239270073753 28.371074859325834 29.307708742853123 20.93697712774729 46-47 21.449313608223168 28.138653533249432 29.042981335681855 21.369051522845545 48-49 21.58884922362644 28.178973314809657 28.982518791156647 21.249658670407257 50-51 21.663296158834402 28.524413915418762 28.522963194687513 21.289326731059326 52-53 21.092798300116257 28.794876551166894 28.592621148277658 21.51970400043919 54-55 21.16401505898758 28.757829924766437 28.599997790497422 21.47815722574856 56-57 21.237328621643968 28.727086203003704 28.67081667093487 21.364768504417462 58-59 21.21169677116471 28.68509245199442 28.809350573730864 21.293860203110004 60-61 21.18882111360853 28.87922067237464 28.719648703055206 21.212309510961624 62-63 21.22017566046251 28.679546119550288 28.691448578895944 21.408829641091256 64-65 20.997567833318495 28.610823652589872 28.751771579701746 21.639836934389884 66-67 21.017959673371447 28.54620912552183 28.5502491338467 21.885582067260017 68-69 21.02012797434162 28.59419252914056 28.610740079904573 21.774939416613247 70-71 21.163033120488066 28.356544931235916 28.733649285410962 21.74677266286506 72-73 21.23310570070538 28.473833578026063 28.713346772617363 21.57971394865119 74-75 21.15679587270936 28.876380573936117 28.32194705179793 21.6448765015566 76-77 21.083104927177512 28.72808441225427 28.413259197566155 21.775551463002063 78-79 21.028574237842133 28.738573359604107 28.37997540933529 21.852876993218477 80-81 20.966008466443782 28.718289530708276 28.31847449232293 21.997227510525015 82-83 21.253027739006082 28.379142639224625 28.520677665596715 21.847151956172574 84-85 21.39562417770469 28.568461773293212 28.135964427451977 21.89994962155012 86-87 20.998883658185445 28.614546408780672 28.469402783514603 21.917167149519283 88-89 21.320781519638327 28.40041666046214 28.247206250001476 22.031595569898055 90-91 21.278926498121617 28.439513742087087 28.30488964780275 21.976670111988543 92-93 21.17395820672537 28.54597598230401 28.23331873371177 22.04674707725885 94-95 20.962852798946635 28.37801291129288 28.306375602108623 22.352758687651857 96-97 21.034904875275 28.46550141905213 28.205491735256327 22.29410197041654 98-99 20.922472539498234 28.542390816451444 27.96116207724182 22.573974566808495 100-101 21.31130619485452 28.534702544377392 27.756969143271647 22.39702211749643 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 162415.0 1 147217.5 2 97702.5 3 49569.5 4 30654.0 5 23938.0 6 22097.5 7 22211.5 8 22891.0 9 22728.5 10 20992.0 11 18148.0 12 14669.5 13 11230.5 14 8471.0 15 6819.5 16 6240.0 17 6487.0 18 7421.5 19 8688.5 20 10140.0 21 11770.5 22 13638.0 23 15689.5 24 17674.0 25 19867.5 26 22770.5 27 26079.0 28 29501.0 29 33569.5 30 38121.5 31 42724.0 32 48079.0 33 53301.5 34 58059.5 35 62926.0 36 67639.5 37 72144.0 38 74657.5 39 75084.5 40 74519.5 41 74051.5 42 74369.0 43 74911.5 44 75658.0 45 76585.0 46 76341.5 47 75286.0 48 74051.0 49 71862.0 50 68267.5 51 63936.5 52 59436.0 53 54259.5 54 48718.5 55 42193.5 56 35472.5 57 29102.0 58 23396.0 59 18811.5 60 14557.0 61 10813.5 62 7845.0 63 5466.5 64 3660.0 65 2402.5 66 1582.0 67 1002.5 68 647.5 69 412.0 70 246.5 71 166.5 72 104.5 73 72.5 74 48.0 75 29.5 76 19.5 77 15.0 78 15.0 79 9.5 80 5.5 81 5.5 82 3.5 83 3.0 84 3.5 85 2.0 86 0.5 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.466911886187702 2 29.580480866569143 3 23.32704513981718 4 29.69305254746935 5 32.858126639010635 6 25.35765352253987 7 24.30121448247066 8 20.29493179613036 9 12.008409457523154 10-11 15.699844397249615 12-13 15.768371187657518 14-15 19.935457150667318 16-17 23.31808972090634 18-19 27.32870930822862 20-21 29.402405083824224 22-23 29.01546339880865 24-25 27.53443987515733 26-27 28.961317847154845 28-29 25.91334008212701 30-31 27.811550950889007 32-33 26.795063968350764 34-35 24.827368811561463 36-37 27.82041249748422 38-39 27.96657169291587 40-41 24.977433095323207 42-43 25.842034671176545 44-45 24.518003208180257 46-47 27.860777593203945 48-49 23.200786875298515 50-51 28.821898683985232 52-53 22.37001688199724 54-55 23.525697734147595 56-57 25.662250412287207 58-59 24.937274518697862 60-61 24.465397162502743 62-63 24.286720596937812 64-65 22.82310100060379 66-67 23.322276426183315 68-69 24.3237438382212 70-71 22.807030060168398 72-73 22.672285738317026 74-75 19.925525459673953 76-77 22.206716601732058 78-79 21.33109441602408 80-81 22.3692471290091 82-83 23.249356411404058 84-85 20.174399742864953 86-87 21.729751366029937 88-89 20.569320574708847 90-91 21.694098660554705 92-93 21.68531221181199 94-95 20.721825538676896 96-97 19.61940783841345 98-99 19.02378799575847 100-101 20.697775451413193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2663192.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.07799444853846 #Duplication Level Percentage of deduplicated Percentage of total 1 86.74200867511276 62.521900197398516 2 8.230534110556496 11.864807838583951 3 2.4260561363005935 5.245957821723504 4 0.9829614300772982 2.833995540009557 5 0.4769861914486929 1.7190104029634194 6 0.2790004651371183 1.2065876386375713 7 0.17814130748353146 0.8988047720297355 8 0.12854313076756435 0.7412104852689805 9 0.09944940496564993 0.6451302293122112 >10 0.42754098795645923 5.318234562194654 >50 0.020201970685732415 1.0012318788013295 >100 0.008038433502963307 1.0231957364856028 >500 3.764292035778647E-4 0.1960887338417656 >1k 1.0755120102146297E-4 0.14616537167684138 >5k 0.0 0.0 >10k+ 5.3775600510731486E-5 4.637678791072413 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 119650 4.492729025920775 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.754892625090493E-5 0.0 0.0 0.0 0.0 6 3.754892625090493E-5 0.0 0.0 0.0 0.0 7 3.754892625090493E-5 0.0 0.0 3.754892625090493E-5 0.0 8 3.754892625090493E-5 0.0 0.0 3.754892625090493E-5 0.0 9 3.754892625090493E-5 0.0 0.0 7.509785250180986E-5 0.0 10-11 3.754892625090493E-5 0.0 0.0 1.1264677875271479E-4 0.0 12-13 3.754892625090493E-5 1.8774463125452464E-5 0.0 1.3142124187816724E-4 0.0 14-15 3.754892625090493E-5 7.509785250180986E-5 0.0 1.5019570500361971E-4 0.0 16-17 3.754892625090493E-5 1.1264677875271479E-4 0.0 1.5019570500361971E-4 0.0 18-19 3.754892625090493E-5 1.1264677875271479E-4 0.0 1.5019570500361971E-4 0.0 20-21 3.754892625090493E-5 1.3142124187816724E-4 0.0 1.689701681290722E-4 0.0 22-23 3.754892625090493E-5 1.5019570500361971E-4 0.0 1.8774463125452464E-4 0.0 24-25 3.754892625090493E-5 1.5019570500361971E-4 0.0 3.0039141000723943E-4 0.0 26-27 3.754892625090493E-5 1.5019570500361971E-4 0.0 4.6936157813631167E-4 0.0 28-29 3.754892625090493E-5 1.5019570500361971E-4 0.0 6.571062093908362E-4 0.0 30-31 3.754892625090493E-5 1.5019570500361971E-4 0.0 0.0020839654069252237 0.0 32-33 3.754892625090493E-5 1.5019570500361971E-4 0.0 0.005895181421392073 0.0 34-35 3.754892625090493E-5 1.5019570500361971E-4 0.0 0.011039384317766049 3.754892625090493E-5 36-37 3.754892625090493E-5 1.5019570500361971E-4 0.0 0.01986338198672871 3.754892625090493E-5 38-39 3.754892625090493E-5 1.8774463125452464E-4 0.0 0.03246104674390731 3.754892625090493E-5 40-41 3.754892625090493E-5 2.2529355750542957E-4 0.0 0.046279051604240326 3.754892625090493E-5 42-43 3.754892625090493E-5 2.628424837563345E-4 0.0 0.06159901351460954 3.754892625090493E-5 44-45 3.754892625090493E-5 2.628424837563345E-4 0.0 0.08009185969318022 3.754892625090493E-5 46-47 3.754892625090493E-5 2.628424837563345E-4 0.0 0.10277141114872679 3.754892625090493E-5 48-49 3.754892625090493E-5 2.628424837563345E-4 0.0 0.13072658674252552 3.754892625090493E-5 50-51 3.754892625090493E-5 2.628424837563345E-4 0.0 0.1622489103301602 3.754892625090493E-5 52-53 3.754892625090493E-5 2.628424837563345E-4 0.0 0.19850239862540892 3.754892625090493E-5 54-55 3.754892625090493E-5 2.628424837563345E-4 0.0 0.23871729864012808 3.754892625090493E-5 56-57 3.754892625090493E-5 2.628424837563345E-4 0.0 0.2882443323650717 3.754892625090493E-5 58-59 3.754892625090493E-5 2.628424837563345E-4 0.0 0.3437791942901601 3.754892625090493E-5 60-61 3.754892625090493E-5 2.628424837563345E-4 0.0 0.4030126254509626 3.754892625090493E-5 62-63 3.754892625090493E-5 2.628424837563345E-4 0.0 0.46669560437249735 3.754892625090493E-5 64-65 3.754892625090493E-5 2.628424837563345E-4 0.0 0.5320119615859464 3.754892625090493E-5 66-67 3.754892625090493E-5 2.628424837563345E-4 0.0 0.6059270229108529 3.754892625090493E-5 68-69 3.754892625090493E-5 2.628424837563345E-4 0.0 0.6901117155653816 3.754892625090493E-5 70-71 3.754892625090493E-5 2.628424837563345E-4 0.0 0.787513630260229 3.754892625090493E-5 72-73 3.754892625090493E-5 2.628424837563345E-4 0.0 0.8885953397276651 3.754892625090493E-5 74-75 3.754892625090493E-5 2.628424837563345E-4 0.0 0.9915920444338974 3.754892625090493E-5 76-77 3.754892625090493E-5 3.0039141000723943E-4 0.0 1.1050461251010066 3.754892625090493E-5 78-79 3.754892625090493E-5 3.0039141000723943E-4 0.0 1.2364861414422994 3.754892625090493E-5 80-81 3.754892625090493E-5 3.191658731326919E-4 0.0 1.3792283845851143 3.754892625090493E-5 82-83 3.754892625090493E-5 3.379403362581444E-4 0.0 1.5281662005593288 3.754892625090493E-5 84-85 3.754892625090493E-5 3.379403362581444E-4 0.0 1.6722038816578002 3.754892625090493E-5 86-87 3.754892625090493E-5 3.379403362581444E-4 0.0 1.8258728623396285 3.754892625090493E-5 88-89 3.754892625090493E-5 3.379403362581444E-4 0.0 2.000118654606953 3.754892625090493E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2060 0.0 54.51664 1 GTATCAA 4170 0.0 49.435863 1 ATCAACG 5080 0.0 41.379353 3 TCAACGC 5395 0.0 39.42939 4 TATCAAC 5330 0.0 38.380455 2 CAACGCA 5415 0.0 38.344166 5 AACGCAG 5835 0.0 35.593998 6 ACGCAGA 6405 0.0 31.344118 7 CGCAGAG 6575 0.0 30.133749 8 GTACATG 4865 0.0 26.562553 1 GCAGAGT 6990 0.0 25.865152 9 TACATGG 4880 0.0 25.635065 2 ACATGGG 4820 0.0 25.51844 3 GTACTAG 735 0.0 23.721586 1 GAGTACT 4485 0.0 21.527687 12-13 GAGTACA 3515 0.0 21.154112 1 AGAGTAC 6285 0.0 21.122145 10-11 GTGGTAT 875 0.0 21.098257 1 CATGGGG 3420 0.0 20.782225 4 CGATAGT 150 4.6110054E-4 20.512194 1 >>END_MODULE