FastQCFastQC Report
Sat 18 Jun 2016
SRR1931022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2663192
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74260.27883832633922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65050.24425576526213655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.1440752300247222No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA27480.10318444933748674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.056.6963121
GTATCAA76100.040.658121
GTGGTAT17750.033.202771
ATCAACG98000.031.3771023
CAACGCA98450.031.2285085
TATCAAC101200.030.5733682
TCAACGC101750.030.3135454
AACGCAG103850.030.01536
GTGTACG3650.027.3449651
ACGCAGA115100.026.8337467
TGGTATC22200.026.7608452
CGCAGAG116250.026.5682958
GGCGTCG6350.023.1613378
ACGGGCG6600.023.0037175
GCAGAGT129850.022.9087439
ACATGGG73950.022.750963
GTACATG80250.022.6832181
CGGGCGT6550.022.4545426
TACATGG79800.022.4533562
GTACTAG10000.022.3382321
Produced by FastQC (version 0.11.5)