##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931022_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2663192 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32632495141169 31.0 31.0 34.0 30.0 34.0 2 31.47771208384525 31.0 31.0 34.0 30.0 34.0 3 31.540743588896333 31.0 31.0 34.0 30.0 34.0 4 35.318465961147375 37.0 35.0 37.0 33.0 37.0 5 35.19143043385532 37.0 35.0 37.0 33.0 37.0 6 35.21976673105056 37.0 35.0 37.0 32.0 37.0 7 35.102146972505174 37.0 35.0 37.0 32.0 37.0 8 35.10628148477466 37.0 35.0 37.0 32.0 37.0 9 36.604477634357565 39.0 35.0 39.0 32.0 39.0 10-11 36.54356895785208 38.0 35.0 39.0 32.0 39.0 12-13 36.42484845253365 38.0 35.0 39.0 32.0 39.0 14-15 37.47085696412425 39.0 36.0 41.0 32.0 41.0 16-17 37.42662470448995 39.0 36.0 41.0 32.0 41.0 18-19 37.45642785048919 39.0 36.0 41.0 32.0 41.0 20-21 37.445433712627555 39.0 36.0 41.0 32.0 41.0 22-23 37.33476219514027 39.0 36.0 40.5 31.5 41.0 24-25 37.205774874661685 39.0 36.0 40.5 31.0 41.0 26-27 36.93605981093364 39.0 36.0 40.0 31.0 41.0 28-29 36.75195029122948 39.0 35.5 40.0 30.0 41.0 30-31 36.52115694249608 38.0 35.0 40.0 30.0 41.0 32-33 36.346337590380266 38.0 35.0 40.0 30.0 41.0 34-35 36.42135189652117 38.0 35.0 40.0 30.0 41.0 36-37 36.488088541870056 38.0 35.0 40.0 30.0 41.0 38-39 36.46175397793325 38.5 35.0 40.0 30.0 41.0 40-41 36.340928667553825 38.5 35.0 40.0 30.0 41.0 42-43 36.1960917575601 38.0 35.0 40.0 30.0 41.0 44-45 36.00796806989507 38.0 35.0 40.0 29.5 41.0 46-47 35.74750844099862 38.0 35.0 40.0 28.5 41.0 48-49 35.50662287961214 38.0 34.0 40.0 27.5 41.0 50-51 35.232060812739 38.0 34.0 40.0 26.5 41.0 52-53 35.009929813547046 38.0 34.0 40.0 26.0 41.0 54-55 34.84413759879122 38.0 34.0 40.0 26.0 41.0 56-57 34.6533798539497 38.0 33.0 40.0 26.0 41.0 58-59 34.43605361536082 37.5 33.0 40.0 25.5 41.0 60-61 34.12023710644971 37.0 33.0 40.0 24.5 41.0 62-63 33.863183352908834 37.0 33.0 40.0 24.0 41.0 64-65 33.54556787494104 36.5 32.0 39.0 23.0 41.0 66-67 33.224524743240444 36.0 32.0 39.0 23.0 40.5 68-69 32.86982406826094 35.5 31.0 39.0 22.0 40.0 70-71 32.50243054199622 35.0 31.0 38.5 22.0 40.0 72-73 32.09182796433753 35.0 31.0 38.0 21.5 40.0 74-75 31.576610886485092 35.0 30.5 37.0 20.0 39.0 76-77 30.20375117528139 33.5 28.5 35.5 18.5 38.5 78-79 30.945082254677843 34.0 30.0 36.0 19.5 39.0 80-81 30.9208252728305 34.0 30.0 36.0 20.0 38.5 82-83 30.65730427997681 34.0 30.0 35.5 18.5 37.0 84-85 30.329092495021015 34.0 30.0 35.0 18.0 37.0 86-87 29.990792627794015 34.0 30.0 35.0 16.0 36.5 88-89 29.643518754937684 34.0 29.0 35.0 11.0 36.0 90-91 29.305590058846676 34.0 29.0 35.0 7.0 36.0 92-93 28.97907867701615 34.0 29.0 35.0 2.0 35.0 94-95 28.58981046053007 34.0 29.0 35.0 2.0 35.0 96-97 28.157280060919376 33.5 29.0 35.0 2.0 35.0 98-99 27.655013607730872 33.0 27.5 35.0 2.0 35.0 100-101 26.507290124031613 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3195719772346415 1101 2 0.3797548219641982 1101 3 0.27229462820777783 1101 4 0.11226834065970337 1101 5 0.2652659752763 1101 6 0.30814720020509156 1101 7 0.3998307386089195 1101 8 0.41607872378141764 1101 9 0.49313099730458276 1101 10-11 0.4172478198051337 1101 12-13 0.43597439100622637 1101 14-15 0.49616185013674396 1101 16-17 0.6443016438595279 1101 18-19 0.4837130574989672 1101 20-21 0.4082031051242012 1101 22-23 0.43723683232471444 1101 24-25 0.39511016344933836 1101 26-27 0.38036807094761826 1101 28-29 0.5324967652670054 1101 30-31 0.44437920624464056 1101 32-33 0.5125567477691391 1101 34-35 0.5646556070680404 1101 36-37 0.5871439809929697 1101 38-39 0.5168517902064877 1101 40-41 0.5908929980850601 1101 42-43 0.4925872804149165 1101 44-45 0.6244915127137247 1101 46-47 0.5384997603112041 1101 48-49 0.6906076426532763 1101 50-51 0.5378772152051923 1101 52-53 0.3317257945183769 1101 54-55 0.4633891652504616 1101 56-57 0.6349844084543648 1101 58-59 0.5481340960829328 1101 60-61 0.5159299852723791 1101 62-63 0.4871320279165161 1101 64-65 0.471370528353134 1101 66-67 0.4633365258565121 1101 68-69 0.41733596296051445 1101 70-71 0.4441682018045512 1101 72-73 0.39154226235273626 1101 74-75 0.4267703427835734 1101 76-77 0.3662709838409448 1101 78-79 0.50043479848987 1101 80-81 0.5184931730624847 1101 82-83 0.4331026910926674 1101 84-85 0.40416118852611405 1101 86-87 0.4812431662624981 1101 88-89 0.6410218796208405 1101 90-91 0.5236882466966826 1101 92-93 0.2803415964765641 1101 94-95 0.4283994453657378 1101 96-97 0.5297337773292021 1101 98-99 0.6504049734232034 1101 100-101 0.8309027385609227 1102 1 0.297026699496179 1102 2 0.3747178590309943 1102 3 0.37861313832250687 1102 4 0.15181516950597285 1102 5 0.2545701510548213 1102 6 0.2830380006178075 1102 7 0.3481569631737145 1102 8 0.36385381429124664 1102 9 0.4150444468587722 1102 10-11 0.3703104016976866 1102 12-13 0.43769270800289917 1102 14-15 0.5712026822792566 1102 16-17 0.529934122618819 1102 18-19 0.5291906052420359 1102 20-21 0.46926235253445725 1102 22-23 0.45479786368015596 1102 24-25 0.5604141417726325 1102 26-27 0.5763702018661405 1102 28-29 0.5117284418787236 1102 30-31 0.4070790725914293 1102 32-33 0.5751947809553144 1102 34-35 0.6117816348624316 1102 36-37 0.6562552355983513 1102 38-39 0.4767232959338301 1102 40-41 0.689674040214932 1102 42-43 0.6648841554862486 1102 44-45 0.8050624607727315 1102 46-47 0.7646014720083159 1102 48-49 0.6744111067940395 1102 50-51 0.594796465719952 1102 52-53 0.5391886033132351 1102 54-55 0.7132838034851048 1102 56-57 0.591671806017338 1102 58-59 0.7240506507061184 1102 60-61 0.6606100935417629 1102 62-63 0.4769876944249134 1102 64-65 0.4911722627632997 1102 66-67 0.4427371237007094 1102 68-69 0.4546526463290732 1102 70-71 0.6331539667666277 1102 72-73 0.5673530272006815 1102 74-75 0.5644972878887451 1102 76-77 0.593416254959525 1102 78-79 0.5942545641002468 1102 80-81 0.5715093301501 1102 82-83 0.4043554075287048 1102 84-85 0.5065061262535338 1102 86-87 0.45486655808671017 1102 88-89 0.4287187070974774 1102 90-91 0.4459304791733878 1102 92-93 0.5452849907432871 1102 94-95 0.5788657478625758 1102 96-97 0.861505207498535 1102 98-99 0.9286248774805053 1102 100-101 1.1496240048310042 1103 1 0.30996747480607567 1103 2 0.38118671447496766 1103 3 0.36431538125650675 1103 4 0.1491843632621297 1103 5 0.2323805324249193 1103 6 0.3004833369018982 1103 7 0.2629302349511704 1103 8 0.2530050362712828 1103 9 0.5555569686440265 1103 10-11 0.43394799870056744 1103 12-13 0.4225271623574969 1103 14-15 0.5057969138113805 1103 16-17 0.49340941669189675 1103 18-19 0.4779233135447356 1103 20-21 0.43504898686311577 1103 22-23 0.5697169601548424 1103 24-25 0.5486548404162619 1103 26-27 0.6180609660326866 1103 28-29 0.5110220926193065 1103 30-31 0.45862425164281717 1103 32-33 0.5674187406119202 1103 34-35 0.5226689410238095 1103 36-37 0.5355195876792607 1103 38-39 0.514475302851622 1103 40-41 0.5581994041568308 1103 42-43 0.7353396038056061 1103 44-45 0.7259837987327842 1103 46-47 0.6454761778238094 1103 48-49 0.4352018418764274 1103 50-51 0.528342316715495 1103 52-53 0.6322711164958221 1103 54-55 0.6322775503928213 1103 56-57 0.6787930434643457 1103 58-59 0.5547063060689723 1103 60-61 0.5662401749105186 1103 62-63 0.36893276382571116 1103 64-65 0.3562583005882942 1103 66-67 0.35782045926271877 1103 68-69 0.2436343918461077 1103 70-71 0.39383319867762623 1103 72-73 0.33256676784584016 1103 74-75 0.37077323797097606 1103 76-77 0.42337663476955 1103 78-79 0.6016047194385798 1103 80-81 0.41173594136744285 1103 82-83 0.43251340578142816 1103 84-85 0.49246181081196383 1103 86-87 0.5521099294634517 1103 88-89 0.5100640663368772 1103 90-91 0.5633048740893436 1103 92-93 0.4348570135737866 1103 94-95 0.6302201666475931 1103 96-97 0.78367460094357 1103 98-99 0.7903895694336676 1103 100-101 1.1031060796341023 1104 1 0.2561040512484638 1104 2 0.3584460671948122 1104 3 0.40319163689878934 1104 4 0.10365687400039292 1104 5 0.2435375570523135 1104 6 0.3536100553950021 1104 7 0.30531608282277745 1104 8 0.3228731162547973 1104 9 0.5444533691930573 1104 10-11 0.3537606249577223 1104 12-13 0.49685062087043264 1104 14-15 0.5239928058571763 1104 16-17 0.5085634880719496 1104 18-19 0.6007588407246942 1104 20-21 0.5097231234138562 1104 22-23 0.6097713815300523 1104 24-25 0.5364074199886417 1104 26-27 0.5499753989846923 1104 28-29 0.6792591782879853 1104 30-31 0.6819071443791174 1104 32-33 0.5868996487435112 1104 34-35 0.6098467781718213 1104 36-37 0.697038959527724 1104 38-39 0.7660570696824678 1104 40-41 0.5910514690398898 1104 42-43 0.5428936827374926 1104 44-45 0.5829117787830995 1104 46-47 0.4577423171478259 1104 48-49 0.4645802660586753 1104 50-51 0.409383328371419 1104 52-53 0.43434509791269704 1104 54-55 0.4981829685492656 1104 56-57 0.5717912523150162 1104 58-59 0.6034567795646666 1104 60-61 0.5671004005897089 1104 62-63 0.49538232342953137 1104 64-65 0.4584068362363425 1104 66-67 0.41147922020834926 1104 68-69 0.4455666961221638 1104 70-71 0.47078734501360486 1104 72-73 0.5516776765036511 1104 74-75 0.6112467858807058 1104 76-77 0.7828031456275149 1104 78-79 0.896433965745917 1104 80-81 0.7667964504009213 1104 82-83 0.7775227511703697 1104 84-85 0.7106096603682737 1104 86-87 0.7868470996885151 1104 88-89 0.8158579262978414 1104 90-91 0.7717337601583516 1104 92-93 0.8636593295752668 1104 94-95 0.8143966336205928 1104 96-97 0.957336551332066 1104 98-99 0.9563502605546041 1104 100-101 1.0276823838410714 1105 1 0.279556819978108 1105 2 0.42681144844240393 1105 3 0.38700422956921443 1105 4 0.3413531601783433 1105 5 0.3262690433983906 1105 6 0.3389042073803381 1105 7 0.30834691653554813 1105 8 0.3159498327737751 1105 9 0.4858132748589412 1105 10-11 0.429401875118252 1105 12-13 0.46707583189631663 1105 14-15 0.4852948034521205 1105 16-17 0.5675088840300617 1105 18-19 0.5159509530850244 1105 20-21 0.46080014348320475 1105 22-23 0.47318363796006935 1105 24-25 0.5084066494376955 1105 26-27 0.45073135616073756 1105 28-29 0.4819958016403447 1105 30-31 0.46615988508872874 1105 32-33 0.5752672482438186 1105 34-35 0.6103266212596239 1105 36-37 0.6420350483979362 1105 38-39 0.6482923679207033 1105 40-41 0.5735459525956372 1105 42-43 0.5076135407693201 1105 44-45 0.5727323338132777 1105 46-47 0.5412817144835955 1105 48-49 0.6053721822787708 1105 50-51 0.582001333578475 1105 52-53 0.5201973155816688 1105 54-55 0.5607166801530568 1105 56-57 0.5859483746574838 1105 58-59 0.7579989438788175 1105 60-61 0.5813912018459462 1105 62-63 0.539157205803761 1105 64-65 0.5508793707651947 1105 66-67 0.6045232466872861 1105 68-69 0.4650184374505457 1105 70-71 0.4804927844027631 1105 72-73 0.7239681975595431 1105 74-75 0.7832897966334009 1105 76-77 0.7034620834347152 1105 78-79 0.8190829097408745 1105 80-81 0.7420214863015886 1105 82-83 0.7617708218742578 1105 84-85 0.7931882040269898 1105 86-87 0.7125630699405789 1105 88-89 0.7157960487220407 1105 90-91 0.7564371564200094 1105 92-93 0.7547699736858071 1105 94-95 0.8171916320444659 1105 96-97 0.8503414665130897 1105 98-99 0.8328793955527942 1105 100-101 0.9860276840640623 1106 1 0.16552484839728976 1106 2 0.2128431399058286 1106 3 0.23677536092517215 1106 4 0.09482820744815257 1106 5 0.10409520508978432 1106 6 0.17032573949516205 1106 7 0.18264932872829576 1106 8 0.133958834728233 1106 9 0.27804320030951857 1106 10-11 0.2510441270074537 1106 12-13 0.2811052569553709 1106 14-15 0.31245664127688144 1106 16-17 0.26015612073562266 1106 18-19 0.24653308796155216 1106 20-21 0.17274205732163495 1106 22-23 0.2547796068161077 1106 24-25 0.2586842203105846 1106 26-27 0.22355837730323458 1106 28-29 0.34697221448245585 1106 30-31 0.2799518469927662 1106 32-33 0.25720254112565755 1106 34-35 0.29126399592301055 1106 36-37 0.20126937296486602 1106 38-39 0.3278056002844494 1106 40-41 0.33000679664027643 1106 42-43 0.38643762067916754 1106 44-45 0.4560341539397115 1106 46-47 0.3273745224694835 1106 48-49 0.23492552236039188 1106 50-51 0.038498783873556874 1106 52-53 0.10305031124620712 1106 54-55 0.1095755200850661 1106 56-57 0.22308939466677913 1106 58-59 0.30589710847324625 1106 60-61 0.37351194517565034 1106 62-63 0.26462592496697823 1106 64-65 0.19533750276415418 1106 66-67 0.17911723562264115 1106 68-69 0.17953233827626036 1106 70-71 0.24060597729542366 1106 72-73 0.3247111452858107 1106 74-75 0.4619868687699942 1106 76-77 0.21661932478768264 1106 78-79 0.29768926203943025 1106 80-81 0.2822842236851635 1106 82-83 0.35672425235739524 1106 84-85 0.4215628228445141 1106 86-87 0.3298294424839803 1106 88-89 0.35103354370857787 1106 90-91 0.2728819573775034 1106 92-93 0.4460793315166818 1106 94-95 0.3679851918909094 1106 96-97 0.36058835179865056 1106 98-99 0.4601440491940032 1106 100-101 0.7294748469204961 1107 1 0.17303124678586812 1107 2 0.1724278790050704 1107 3 0.15342056910383306 1107 4 -0.014111367329199709 1107 5 0.07605569641084742 1107 6 0.09820329141060569 1107 7 0.25095373226064766 1107 8 0.09847244755445672 1107 9 0.1322432686007673 1107 10-11 0.19947640433256453 1107 12-13 0.1950783351676364 1107 14-15 0.20156606703520907 1107 16-17 0.2519686894576054 1107 18-19 0.2554429027920335 1107 20-21 0.286800029698135 1107 22-23 0.26995498688195596 1107 24-25 0.21354120027117318 1107 26-27 0.2651280108799696 1107 28-29 0.2532252492138838 1107 30-31 0.41483144451297704 1107 32-33 0.3233329022234557 1107 34-35 0.3283899995380679 1107 36-37 0.4007794302605987 1107 38-39 0.2998597571224977 1107 40-41 0.3474038350556654 1107 42-43 0.26465114673165857 1107 44-45 0.4562562571221491 1107 46-47 0.5093706944005234 1107 48-49 0.24235517141229224 1107 50-51 0.28890524209528934 1107 52-53 0.2473634986618265 1107 54-55 0.4749910159327726 1107 56-57 0.49659865627551625 1107 58-59 0.4269583795049101 1107 60-61 0.33834915826626855 1107 62-63 0.17487149151804715 1107 64-65 0.2091401661227721 1107 66-67 0.2290268593629392 1107 68-69 0.21677926125438063 1107 70-71 0.2994323076433645 1107 72-73 0.38942305333697647 1107 74-75 0.5318817065156232 1107 76-77 0.32796569611037185 1107 78-79 0.4387648804981126 1107 80-81 0.45557044232366195 1107 82-83 0.488800462882395 1107 84-85 0.5054542334973178 1107 86-87 0.5194726677103922 1107 88-89 0.5586702593381894 1107 90-91 0.6304145131930632 1107 92-93 0.4398015490399665 1107 94-95 0.6439538781615504 1107 96-97 0.752853271713068 1107 98-99 0.7301457094857788 1107 100-101 0.9805062640265163 1108 1 0.1084315545853265 1108 2 0.169861011257737 1108 3 0.04589745606055118 1108 4 0.05508509634709924 1108 5 -0.013038766568968185 1108 6 0.1518873363897697 1108 7 0.036171494760786516 1108 8 0.11997010535784369 1108 9 0.14055610069279112 1108 10-11 0.1901340167523884 1108 12-13 0.1759610680673518 1108 14-15 0.19304541414089016 1108 16-17 0.2062756351658308 1108 18-19 0.18104923402469097 1108 20-21 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-1.1683366942507583 2316 100-101 -1.5609798699759878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 33.0 5 73.0 6 152.0 7 398.0 8 964.0 9 1711.0 10 3203.0 11 5149.0 12 7440.0 13 9793.0 14 11635.0 15 14906.0 16 18185.0 17 21468.0 18 18588.0 19 14262.0 20 13596.0 21 14025.0 22 15709.0 23 18339.0 24 22965.0 25 28404.0 26 36895.0 27 47726.0 28 60478.0 29 74955.0 30 92055.0 31 110941.0 32 133795.0 33 161489.0 34 201552.0 35 249994.0 36 328702.0 37 442650.0 38 405991.0 39 74947.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2252594099351 22.00665173899378 14.78391611463942 22.984172736431695 2 15.058300722905681 21.91984012034534 40.80584963666958 22.2160095200794 3 18.442122954996307 26.88491053971313 30.98267193914545 23.690294566145116 4 12.31364896723652 18.38057962336367 39.73585507460059 29.569916334799217 5 11.94757921282455 38.906186752339906 36.3899108646854 12.756323170150146 6 28.845601440984623 40.13996055090552 17.95654741945622 13.057890588653636 7 25.29335120663565 33.58329672310275 23.52457015834394 17.598781911917662 8 24.15688168162936 37.663709423962864 20.53609614674793 17.643312747659845 9 24.978784856668238 16.475455017888308 21.57351028389992 36.97224984154353 10-11 23.717216032490334 27.684391512140316 29.208145713865168 19.39024674150418 12-13 24.312798326219063 25.067907233124764 29.683496345738497 20.935798094917676 14-15 21.476634054172585 26.777002934824072 27.22167609395042 24.52468691705292 16-17 20.50325323897038 29.847679025770578 28.238538565751174 21.410529169507868 18-19 20.341154524345225 28.65403620918056 30.631813252668227 20.372996013805988 20-21 21.08173199679182 27.966233752579612 30.558085935974578 20.393948314653993 22-23 20.915457008501292 27.758094776420776 30.403500171880758 20.922948043197174 24-25 20.74525707097731 27.94784420851052 30.66955795244398 20.63734076806819 26-27 20.752502603142847 28.14884532356136 30.443497437410983 20.655154635884806 28-29 20.546048980431706 28.268547521652636 30.51613743839146 20.669266059524194 30-31 20.96547795494068 27.905560710515832 30.466635098532755 20.662326236010728 32-33 20.95082235035308 28.129480394007082 30.215246695716758 20.704450559923085 34-35 20.961623101847195 28.08817388273926 30.473191901014403 20.47701111439915 36-37 21.03033622155856 28.050242389754015 30.400674835003933 20.518746553683496 38-39 20.87919246991147 28.12824506018645 30.517548083918395 20.475014385983688 40-41 20.947063044538627 28.011256598860975 30.4524080327462 20.589272323854203 42-43 20.872583165143237 28.054182840235462 30.21287430158629 20.860359693035004 44-45 20.80532603128206 28.50606985515562 29.961610907414055 20.726993206148265 46-47 20.95034952298515 28.271480689394892 29.71210121239319 21.066068575226772 48-49 21.110345037362993 28.380816762294454 29.5791588885772 20.929679311765355 50-51 21.119788340720127 28.57780229697568 29.157002514262757 21.145406848041436 52-53 21.026723546684774 28.801533106572595 29.009297916957603 21.162445429785024 54-55 21.060312590073618 28.766717898114823 29.089072303941126 21.08389720787043 56-57 21.09188255684467 28.756626159466425 28.982234297881977 21.16925698580693 58-59 20.97311204961965 28.71993857444722 29.289334220391023 21.01761515554211 60-61 21.015388877955328 28.841970439534805 29.194657680219084 20.947983002290783 62-63 21.01230740164071 28.713268516333528 29.07660803328843 21.197816048737327 64-65 20.740405479410807 28.664793524674852 29.208707287375486 21.386093708538855 66-67 20.872130140451468 28.6076137102797 29.08943692934062 21.430819219928207 68-69 20.859795382528073 28.68051071370699 29.036382733779913 21.42331116998502 70-71 20.842387847593614 28.370217541683385 29.283033013254528 21.504361597468474 72-73 21.04630051852444 28.56036367226247 29.15137810914869 21.241957700064404 74-75 20.968671930900204 28.85690884061051 28.92744457523351 21.24697465325578 76-77 20.819589134932485 28.763734242813115 28.97266048234964 21.44401613990476 78-79 20.79637601934782 28.728593296717264 29.076410273452673 21.398620410482234 80-81 20.75403153585304 28.785242966050877 29.046564816417597 21.41416068167848 82-83 20.888899486030297 28.6553879705256 29.247083950387353 21.20862859305675 84-85 21.1183046509602 28.651539205584875 28.8911389039919 21.339017239463022 86-87 20.615167813661202 28.809207146912424 29.112865313503494 21.46275972592288 88-89 20.83717271392578 28.61183392848426 28.950394076276947 21.600599281313013 90-91 20.829177694130934 28.564843742959194 29.181280247295582 21.424698315614286 92-93 20.889031182282842 28.65855719505921 28.959939610850515 21.492472011807433 94-95 20.476247963157633 28.60243934814285 29.07772603622918 21.843586652470336 96-97 20.634058797499165 28.486573346302734 29.087675445044038 21.791692411154063 98-99 20.419875848230244 28.678931147284914 29.00012466243515 21.90106834204969 100-101 20.58548538745986 28.600566538199274 28.785344804279976 22.02860327006089 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 289.0 1 207.0 2 153.0 3 203.5 4 202.0 5 201.0 6 338.5 7 721.0 8 1229.0 9 1577.0 10 1540.0 11 1420.0 12 1528.0 13 1783.5 14 2119.5 15 2494.5 16 2849.0 17 3177.0 18 3649.5 19 4182.0 20 4740.5 21 5626.5 22 6935.5 23 8667.5 24 10829.5 25 13419.5 26 17568.5 27 22623.0 28 27660.0 29 34213.5 30 41442.5 31 48512.5 32 57618.5 33 66819.0 34 74430.0 35 82112.5 36 91428.5 37 101454.0 38 104447.0 39 102731.5 40 101995.5 41 100376.5 42 99850.0 43 102350.0 44 105411.5 45 109145.5 46 109844.0 47 107501.0 48 106733.5 49 104160.5 50 98233.5 51 91223.0 52 84095.0 53 76899.0 54 69300.0 55 59170.0 56 48496.0 57 37926.5 58 28817.5 59 22626.5 60 16663.0 61 11272.5 62 7421.5 63 4584.5 64 2671.0 65 1564.5 66 829.5 67 442.5 68 263.0 69 130.5 70 65.5 71 43.5 72 28.5 73 17.5 74 16.0 75 11.0 76 4.5 77 3.5 78 2.5 79 1.0 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004843811486366736 2 0.007096747061421032 3 0.002065190943799771 4 0.16897016812907217 5 0.0018774463125452465 6 4.881360412617641E-4 7 7.509785250180986E-5 8 4.881360412617641E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.191658731326919E-4 24-25 6.383317462653838E-4 26-27 0.0024406802063088205 28-29 2.2529355750542957E-4 30-31 0.006345768536402933 32-33 0.020633134974872257 34-35 0.012766634925307675 36-37 8.448508406453609E-4 38-39 3.567147993835968E-4 40-41 0.001614603828788912 42-43 0.010607571665880642 44-45 0.007359589545177366 46-47 0.010401052571500665 48-49 0.009875367603987996 50-51 0.18614880188886118 52-53 0.1116141832808149 54-55 0.01650275308727272 56-57 0.006289445147026575 58-59 0.0171410848335381 60-61 0.11964965349850855 62-63 0.23178952174683615 64-65 0.2379475456519845 66-67 0.248423696075987 68-69 0.24326071871648758 70-71 0.23047530932805443 72-73 0.1578744604219298 74-75 0.2662782105082923 76-77 0.20860305978690233 78-79 0.005763760179513907 80-81 3.754892625090493E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 7.509785250180986E-5 90-91 0.00540704538013031 92-93 0.01734760392791808 94-95 0.002346807890681558 96-97 1.8774463125452464E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2663192.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.93494401630028 #Duplication Level Percentage of deduplicated Percentage of total 1 67.82379918279615 31.154824184345344 2 16.35300033410084 15.023483096909237 3 6.4165582659737455 8.842327341744983 4 3.0827306947855253 5.664202475292143 5 1.7526545323415677 4.025404391151243 6 1.0687194500751265 2.945494086499929 7 0.7266591178798595 2.336533212912184 8 0.4905752459554379 1.8027637166996624 9 0.3594168539816352 1.4858813759545066 >10 1.7268018375222478 14.119351756922036 >50 0.11982583311695959 3.8244225482967438 >100 0.07441702297945992 6.180130756397471 >500 0.003282459994202787 1.0003096679077046 >1k 0.0013950454975293644 1.0697436811035033 >5k 1.6412299970933699E-4 0.525127707863311 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7426 0.27883832633922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6505 0.24425576526213655 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3837 0.1440752300247222 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 2748 0.10318444933748674 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.754892625090493E-5 0.0 5 0.0 0.0 0.0 3.754892625090493E-5 0.0 6 0.0 0.0 0.0 3.754892625090493E-5 0.0 7 0.0 0.0 0.0 3.754892625090493E-5 0.0 8 0.0 0.0 0.0 3.754892625090493E-5 0.0 9 0.0 0.0 0.0 7.509785250180986E-5 0.0 10-11 0.0 0.0 0.0 1.1264677875271479E-4 0.0 12-13 0.0 0.0 0.0 1.1264677875271479E-4 0.0 14-15 0.0 5.632338937635739E-5 0.0 1.689701681290722E-4 0.0 16-17 0.0 1.1264677875271479E-4 0.0 1.8774463125452464E-4 3.754892625090493E-5 18-19 0.0 1.1264677875271479E-4 0.0 1.8774463125452464E-4 7.509785250180986E-5 20-21 0.0 1.1264677875271479E-4 0.0 2.4406802063088205E-4 7.509785250180986E-5 22-23 0.0 1.1264677875271479E-4 0.0 2.81616946881787E-4 7.509785250180986E-5 24-25 0.0 1.1264677875271479E-4 0.0 3.754892625090493E-4 7.509785250180986E-5 26-27 0.0 1.1264677875271479E-4 0.0 7.69752988143551E-4 7.509785250180986E-5 28-29 0.0 1.3142124187816724E-4 0.0 0.0013517613450325775 7.509785250180986E-5 30-31 0.0 1.5019570500361971E-4 0.0 0.0042242542032268045 7.509785250180986E-5 32-33 0.0 1.5019570500361971E-4 0.0 0.011884235158411409 7.509785250180986E-5 34-35 0.0 1.5019570500361971E-4 0.0 0.022266513266786622 7.509785250180986E-5 36-37 0.0 2.2529355750542957E-4 0.0 0.03989573414158649 7.509785250180986E-5 38-39 0.0 3.379403362581444E-4 0.0 0.06657424624285443 7.509785250180986E-5 40-41 0.0 3.754892625090493E-4 0.0 0.09830308892486911 7.509785250180986E-5 42-43 0.0 4.1303818875995424E-4 0.0 0.13307339463320705 7.509785250180986E-5 44-45 0.0 4.1303818875995424E-4 0.0 0.17602936626424232 7.509785250180986E-5 46-47 0.0 4.1303818875995424E-4 0.0 0.22647634868233307 7.509785250180986E-5 48-49 0.0 4.1303818875995424E-4 0.0 0.2849963502443684 7.509785250180986E-5 50-51 0.0 4.1303818875995424E-4 0.0 0.347759380472756 7.509785250180986E-5 52-53 0.0 4.1303818875995424E-4 0.0 0.41221211238243427 7.509785250180986E-5 54-55 0.0 4.318126518854067E-4 0.0 0.48537619518232256 7.509785250180986E-5 56-57 0.0 4.5058711501085914E-4 0.0 0.5752120012376126 7.509785250180986E-5 58-59 0.0 4.5058711501085914E-4 0.0 0.6770446892300668 7.509785250180986E-5 60-61 0.0 4.881360412617641E-4 0.0 0.7874385324077273 7.509785250180986E-5 62-63 0.0 4.881360412617641E-4 0.0 0.9086276918825229 7.509785250180986E-5 64-65 0.0 4.881360412617641E-4 0.0 1.0286903835697914 7.509785250180986E-5 66-67 0.0 4.881360412617641E-4 0.0 1.1664949429106124 7.509785250180986E-5 68-69 0.0 4.881360412617641E-4 0.0 1.3237686205125279 7.509785250180986E-5 70-71 0.0 5.256849675126689E-4 0.0 1.5038720452749934 7.509785250180986E-5 72-73 0.0 5.632338937635739E-4 0.0 1.6955968627121139 7.509785250180986E-5 74-75 0.0 5.820083568890263E-4 0.0 1.8871714844442309 7.509785250180986E-5 76-77 0.0 6.383317462653838E-4 0.0 2.102082763841285 7.509785250180986E-5 78-79 0.0 6.383317462653838E-4 0.0 2.348966953940985 7.509785250180986E-5 80-81 0.0 6.383317462653838E-4 0.0 2.625064208663889 7.509785250180986E-5 82-83 0.0 6.383317462653838E-4 0.0 2.9175891186215637 7.509785250180986E-5 84-85 0.0 6.383317462653838E-4 0.0 3.2029234092021905 7.509785250180986E-5 86-87 0.0 6.383317462653838E-4 0.0 3.5096042643564562 7.509785250180986E-5 88-89 0.0 6.383317462653838E-4 0.0 3.85766403623922 7.509785250180986E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4225 0.0 56.696312 1 GTATCAA 7610 0.0 40.65812 1 GTGGTAT 1775 0.0 33.20277 1 ATCAACG 9800 0.0 31.377102 3 CAACGCA 9845 0.0 31.228508 5 TATCAAC 10120 0.0 30.573368 2 TCAACGC 10175 0.0 30.313545 4 AACGCAG 10385 0.0 30.0153 6 GTGTACG 365 0.0 27.344965 1 ACGCAGA 11510 0.0 26.833746 7 TGGTATC 2220 0.0 26.760845 2 CGCAGAG 11625 0.0 26.568295 8 GGCGTCG 635 0.0 23.161337 8 ACGGGCG 660 0.0 23.003717 5 GCAGAGT 12985 0.0 22.908743 9 ACATGGG 7395 0.0 22.75096 3 GTACATG 8025 0.0 22.683218 1 CGGGCGT 655 0.0 22.454542 6 TACATGG 7980 0.0 22.453356 2 GTACTAG 1000 0.0 22.338232 1 >>END_MODULE