FastQCFastQC Report
Sat 18 Jun 2016
SRR1931021_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931021_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557628
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN669164.296019331958593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.053.9742161
GTATCAA26100.041.925521
ATCAACG28850.035.7278863
TCAACGC30000.034.1890874
CAACGCA29850.033.8491335
TATCAAC31350.033.265392
AACGCAG32500.030.3911916
CGCGCGA350.00645049728.6470418-19
ACGCAGA37100.026.6216757
GGACCGT1258.278601E-626.2142056
CGCAGAG38500.025.8662418
GCAGAGT42350.021.6328879
GTACATG23600.021.4500561
GAGTACT27400.020.67887112-13
GTACTAG2801.4964826E-820.0881461
CAGAGTA39850.019.67883510-11
AGAGTAC36300.019.6451510-11
TACATGG24050.019.555672
GTGTAGC5850.019.2296811
GTATAAC3555.9299055E-1018.7249321
Produced by FastQC (version 0.11.5)