##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931021_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1557628 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.216468245306324 28.0 2.0 31.0 2.0 33.0 2 19.929079343720066 27.0 2.0 31.0 2.0 33.0 3 20.581677396656968 27.0 2.0 31.0 2.0 33.0 4 22.118077615451185 30.0 2.0 35.0 2.0 37.0 5 21.47392509636447 30.0 2.0 35.0 2.0 37.0 6 22.442321915117088 30.0 2.0 35.0 2.0 37.0 7 22.631528195435624 30.0 2.0 35.0 2.0 37.0 8 23.042144209015245 32.0 2.0 35.0 2.0 37.0 9 24.359042723936653 32.0 2.0 37.0 2.0 39.0 10-11 24.70217567994412 32.0 2.0 37.0 2.0 39.0 12-13 24.58402295028081 32.0 2.0 37.0 2.0 39.0 14-15 24.5255802412386 32.0 2.0 38.0 2.0 40.0 16-17 23.36332359202582 31.5 2.0 38.0 2.0 40.0 18-19 22.32021252828018 29.5 2.0 38.0 2.0 40.0 20-21 21.988348951097436 28.0 2.0 37.5 2.0 40.0 22-23 21.625255195720673 27.0 2.0 37.0 2.0 40.0 24-25 21.44967476188153 27.0 2.0 37.0 2.0 39.5 26-27 21.159489300397787 27.0 2.0 37.0 2.0 39.0 28-29 20.959403336355024 26.5 2.0 37.0 2.0 39.0 30-31 20.92992518110871 26.5 2.0 36.5 2.0 39.0 32-33 20.801408295177026 26.0 2.0 36.5 2.0 39.0 34-35 20.468062335808035 25.0 2.0 36.0 2.0 39.0 36-37 20.002202708220448 24.0 2.0 36.0 2.0 39.0 38-39 19.870657820737684 24.0 2.0 35.5 2.0 39.0 40-41 19.740273351531947 24.0 2.0 35.0 2.0 39.0 42-43 19.587271158453753 24.0 2.0 35.0 2.0 39.0 44-45 19.19140288952176 23.0 2.0 35.0 2.0 38.0 46-47 18.839388801433977 21.0 2.0 35.0 2.0 38.0 48-49 18.581205525324403 19.5 2.0 35.0 2.0 38.0 50-51 16.356873078809574 11.0 2.0 33.0 2.0 37.5 52-53 18.26231937278991 19.5 2.0 34.0 2.0 38.0 54-55 19.0140457156651 22.0 2.0 35.5 2.0 39.0 56-57 18.973650961590316 22.0 2.0 36.0 2.0 39.0 58-59 18.68689860480166 20.5 2.0 35.0 2.0 39.0 60-61 18.392157498452775 19.0 2.0 35.0 2.0 39.0 62-63 17.953898812810245 16.0 2.0 34.5 2.0 38.5 64-65 17.590271874927776 13.0 2.0 34.0 2.0 38.0 66-67 17.21884878802898 8.0 2.0 34.0 2.0 38.0 68-69 16.768907274394145 8.0 2.0 33.5 2.0 38.0 70-71 16.40774466047092 7.0 2.0 33.0 2.0 37.0 72-73 15.996553092265932 2.0 2.0 32.0 2.0 36.5 74-75 15.53996974887457 2.0 2.0 32.0 2.0 36.0 76-77 15.11486311237343 2.0 2.0 31.0 2.0 35.5 78-79 14.723416951929472 2.0 2.0 31.0 2.0 35.0 80-81 14.280858780145195 2.0 2.0 30.5 2.0 35.0 82-83 13.810573513059602 2.0 2.0 30.0 2.0 34.0 84-85 13.40080173186409 2.0 2.0 29.5 2.0 34.0 86-87 12.910704609829818 2.0 2.0 29.0 2.0 34.0 88-89 12.478519903340207 2.0 2.0 29.0 2.0 34.0 90-91 11.991926185199546 2.0 2.0 28.5 2.0 34.0 92-93 11.439855023150585 2.0 2.0 26.5 2.0 34.0 94-95 10.858117920325007 2.0 2.0 26.0 2.0 33.5 96-97 10.21637964905613 2.0 2.0 25.0 2.0 33.0 98-99 9.537775065676787 2.0 2.0 21.0 2.0 32.0 100-101 8.615805571034933 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.081862401396364 1101 2 9.305014268936961 1101 3 8.693675655041506 1101 4 10.959930968335616 1101 5 11.582241179712863 1101 6 10.64769491923078 1101 7 10.476985286392246 1101 8 9.702020150040017 1101 9 9.552779939810105 1101 10-11 8.942166737250641 1101 12-13 8.77197921292186 1101 14-15 9.586642133524602 1101 16-17 10.739034107009815 1101 18-19 11.738322616152576 1101 20-21 11.775044870187031 1101 22-23 12.037971946147241 1101 24-25 11.926993983619266 1101 26-27 11.777337559208455 1101 28-29 11.64057493837035 1101 30-31 11.367152310796271 1101 32-33 11.062278657226209 1101 34-35 11.075930213945153 1101 36-37 11.291706709021017 1101 38-39 10.755077987636675 1101 40-41 10.500411700964207 1101 42-43 10.385926289322093 1101 44-45 10.136327312390708 1101 46-47 9.880922805961127 1101 48-49 10.10351561988243 1101 50-51 11.918223394114678 1101 52-53 10.945071091016064 1101 54-55 10.85932249456701 1101 56-57 10.97287933916214 1101 58-59 10.706499243707892 1101 60-61 10.481560460383466 1101 62-63 10.686104817978709 1101 64-65 10.362249763005764 1101 66-67 10.108525626333531 1101 68-69 10.170625199774907 1101 70-71 9.769320879905358 1101 72-73 9.685148684658813 1101 74-75 9.647926050764939 1101 76-77 9.29236931631859 1101 78-79 8.965402307203249 1101 80-81 8.788337324632602 1101 82-83 8.300530777463587 1101 84-85 8.247551534201293 1101 86-87 7.915852824807798 1101 88-89 7.668730197624702 1101 90-91 7.3780668276669505 1101 92-93 7.4505410049570475 1101 94-95 7.172990787958041 1101 96-97 6.9663071141143345 1101 98-99 6.50914061162262 1101 100-101 5.787518868665945 1102 1 7.777195265159772 1102 2 8.867713817461926 1102 3 8.179959801672943 1102 4 10.230186031540622 1102 5 11.000992871435717 1102 6 10.030986978264991 1102 7 9.725461427305092 1102 8 9.063847704223754 1102 9 8.981488308473342 1102 10-11 8.183544184734327 1102 12-13 8.374855964275621 1102 14-15 8.763803276762868 1102 16-17 9.836155262032193 1102 18-19 10.745430843047561 1102 20-21 10.681706257930664 1102 22-23 10.908332699971464 1102 24-25 10.863456050893387 1102 26-27 10.76497115858156 1102 28-29 10.413555825079879 1102 30-31 10.378886324856452 1102 32-33 10.25667593282338 1102 34-35 10.284654419377507 1102 36-37 10.781172977001884 1102 38-39 10.555126066213369 1102 40-41 10.329175139412413 1102 42-43 9.769565665601636 1102 44-45 9.70693571200967 1102 46-47 9.471557091743776 1102 48-49 8.920830861314649 1102 50-51 10.715190854713422 1102 52-53 10.149461868902012 1102 54-55 9.956633571183175 1102 56-57 9.582122015591075 1102 58-59 9.734498098196546 1102 60-61 9.68098505029489 1102 62-63 9.388932294399268 1102 64-65 9.36095339858361 1102 66-67 8.995006723153164 1102 68-69 8.72805255476528 1102 70-71 8.399619056807602 1102 72-73 8.490913477980747 1102 74-75 8.018363820038614 1102 76-77 7.919357137409458 1102 78-79 7.657130025437368 1102 80-81 7.430785062546784 1102 82-83 7.436114980772411 1102 84-85 7.260491625154295 1102 86-87 7.074863931482417 1102 88-89 6.877552167814491 1102 90-91 6.534819998385524 1102 92-93 6.359788952831794 1102 94-95 6.039446185544689 1102 96-97 6.007775073444199 1102 98-99 5.747733913222596 1102 100-101 4.98718011284679 1103 1 7.764689212052364 1103 2 9.272621906486663 1103 3 8.605537679064753 1103 4 10.743103399775396 1103 5 11.303978478738248 1103 6 10.557369271056203 1103 7 9.928595196906425 1103 8 9.46345053152471 1103 9 9.35016438466771 1103 10-11 8.785072196368137 1103 12-13 8.513881402876006 1103 14-15 9.152860554338009 1103 16-17 10.251607049679556 1103 18-19 10.852513830224598 1103 20-21 11.148548187046742 1103 22-23 11.360193465913536 1103 24-25 11.338318254772407 1103 26-27 11.398769452012512 1103 28-29 11.012634271871029 1103 30-31 10.995277029060528 1103 32-33 11.046379689674314 1103 34-35 11.16516015557605 1103 36-37 11.139775905002853 1103 38-39 11.00816468911107 1103 40-41 10.70743234057402 1103 42-43 10.473937823232696 1103 44-45 10.43568227080885 1103 46-47 10.1643370859091 1103 48-49 10.26953760658656 1103 50-51 12.009517371544392 1103 52-53 11.51942557247079 1103 54-55 10.786148263759362 1103 56-57 10.53646529601248 1103 58-59 10.470560031666416 1103 60-61 10.16790052744265 1103 62-63 10.250036433021723 1103 64-65 10.143618150469145 1103 66-67 9.70601582613818 1103 68-69 9.424324284865552 1103 70-71 9.09821577250629 1103 72-73 9.066081595171873 1103 74-75 8.927418195083968 1103 76-77 8.842717948518446 1103 78-79 8.458070163722176 1103 80-81 8.056961623730183 1103 82-83 7.9792356889896805 1103 84-85 7.646225987404822 1103 86-87 7.256303512214954 1103 88-89 6.965796292783205 1103 90-91 6.648133613767749 1103 92-93 6.235309112826345 1103 94-95 5.921415218809754 1103 96-97 5.766091339606181 1103 98-99 5.291853661827975 1103 100-101 4.4295613793863975 1104 1 7.9199921296926306 1104 2 9.485129986055636 1104 3 8.667664577083361 1104 4 10.815650856134841 1104 5 11.56989513893642 1104 6 10.725404356153739 1104 7 10.330074873389965 1104 8 9.743458467819625 1104 9 9.606647445320878 1104 10-11 9.018134300414854 1104 12-13 8.906596696638612 1104 14-15 9.55908477860871 1104 16-17 10.470560648470105 1104 18-19 11.451706272318308 1104 20-21 11.680082989586062 1104 22-23 11.869985794693779 1104 24-25 11.615093456680025 1104 26-27 11.571740963325361 1104 28-29 11.453949034467719 1104 30-31 11.208554073218682 1104 32-33 11.06541772948201 1104 34-35 10.855088605597341 1104 36-37 11.132021151024372 1104 38-39 10.790737775205361 1104 40-41 10.378517349609353 1104 42-43 10.179793633666964 1104 44-45 10.22079729267719 1104 46-47 9.742965891467698 1104 48-49 9.695588784807164 1104 50-51 11.525877455898359 1104 52-53 10.693316214099195 1104 54-55 10.190334550509156 1104 56-57 9.912560435793552 1104 58-59 10.048924959046417 1104 60-61 9.746198170421096 1104 62-63 9.759314063334017 1104 64-65 9.635555085216698 1104 66-67 9.53202072139577 1104 68-69 9.345971871296086 1104 70-71 8.78042374550838 1104 72-73 8.59190193892806 1104 74-75 8.626769363075889 1104 76-77 8.205542101551105 1104 78-79 7.892458985588629 1104 80-81 7.675436396976311 1104 82-83 7.743737744519079 1104 84-85 7.60709892906435 1104 86-87 7.266116697346076 1104 88-89 6.650798110006127 1104 90-91 6.410013572591874 1104 92-93 5.833086224812979 1104 94-95 5.6618811460613525 1104 96-97 5.335806503515334 1104 98-99 5.399630577946269 1104 100-101 4.7533885088923675 1105 1 7.601563597557302 1105 2 8.88005491076877 1105 3 8.470891862313032 1105 4 10.689198542518323 1105 5 10.561912262731301 1105 6 10.254110691534692 1105 7 10.082595132080574 1105 8 9.364331442832139 1105 9 9.357346008699377 1105 10-11 8.800831721266665 1105 12-13 8.363456793541797 1105 14-15 8.990921072872805 1105 16-17 10.095022003563159 1105 18-19 10.692698295789473 1105 20-21 11.051836838560959 1105 22-23 11.16204100271121 1105 24-25 11.274914249255009 1105 26-27 11.433191619572938 1105 28-29 10.969975439528689 1105 30-31 10.796592453651858 1105 32-33 10.760089817273197 1105 34-35 10.767871857002582 1105 36-37 10.643899549512287 1105 38-39 10.612372787300629 1105 40-41 10.27034511018654 1105 42-43 9.995578133388385 1105 44-45 9.956221261758444 1105 46-47 9.9958609976451 1105 48-49 9.997423527491819 1105 50-51 11.59707873101455 1105 52-53 10.50945193498416 1105 54-55 10.313401952530441 1105 56-57 10.014159620509087 1105 58-59 9.901605312524424 1105 60-61 9.710512901257328 1105 62-63 9.881223070252652 1105 64-65 9.778564867219785 1105 66-67 9.590186613448157 1105 68-69 9.20685443789058 1105 70-71 9.239238832502615 1105 72-73 9.07697503947746 1105 74-75 8.542617336501566 1105 76-77 8.25736697054843 1105 78-79 8.243547912248292 1105 80-81 7.965594446499908 1105 82-83 7.88494522595302 1105 84-85 7.619348648722152 1105 86-87 7.445668290888689 1105 88-89 6.9312122406632195 1105 90-91 6.501970043736021 1105 92-93 6.644367265217516 1105 94-95 6.214620389031635 1105 96-97 5.658055094751733 1105 98-99 5.23946032736619 1105 100-101 4.405942620225245 1106 1 7.877256275294478 1106 2 9.102220242546018 1106 3 8.701147655827995 1106 4 10.454634928603209 1106 5 11.24314458330402 1106 6 10.455475231985218 1106 7 9.558758549587253 1106 8 9.20611982698141 1106 9 8.911156937120598 1106 10-11 8.521547829248608 1106 12-13 8.516493296333149 1106 14-15 8.910705841968362 1106 16-17 10.086811633055746 1106 18-19 11.38745753534727 1106 20-21 11.492346966760167 1106 22-23 11.380228352158102 1106 24-25 10.988066141998925 1106 26-27 11.643334762890603 1106 28-29 11.532658727377338 1106 30-31 11.190149936919777 1106 32-33 11.360595248749075 1106 34-35 11.130266225204341 1106 36-37 11.376478086198322 1106 38-39 11.008519517207013 1106 40-41 10.92565436451055 1106 42-43 10.590740864177189 1106 44-45 10.495401541213482 1106 46-47 10.014844477276622 1106 48-49 10.187470387981342 1106 50-51 11.733536690003616 1106 52-53 10.99633082001051 1106 54-55 10.53212355425542 1106 56-57 10.366106270001875 1106 58-59 10.392257943579018 1106 60-61 10.319866319013077 1106 62-63 10.21900571870647 1106 64-65 9.851064306815296 1106 66-67 9.440544204883043 1106 68-69 9.637544678313002 1106 70-71 9.088678783827483 1106 72-73 8.501122068266241 1106 74-75 8.523204213429624 1106 76-77 8.625578159424313 1106 78-79 8.227515896250967 1106 80-81 7.797078682232717 1106 82-83 7.643812426101041 1106 84-85 7.453233906348103 1106 86-87 7.302765680293307 1106 88-89 7.076760316252001 1106 90-91 6.777266702302024 1106 92-93 6.555752371623209 1106 94-95 6.119766503382619 1106 96-97 5.5598743634149805 1106 98-99 5.107152919708398 1106 100-101 4.7500688695714395 1107 1 7.463496996713573 1107 2 8.527294144921207 1107 3 8.419917982050936 1107 4 10.157475109322846 1107 5 10.938347191235167 1107 6 9.798065453225743 1107 7 9.882745650263583 1107 8 8.690848189706127 1107 9 6.264781468850028 1107 10-11 7.939240440274073 1107 12-13 7.817203867758312 1107 14-15 8.500689219634932 1107 16-17 9.43177364165669 1107 18-19 10.852131919342117 1107 20-21 11.03789630014775 1107 22-23 10.465796707557484 1107 24-25 10.468530690259474 1107 26-27 10.906370384081026 1107 28-29 10.751646743720414 1107 30-31 10.57019619036932 1107 32-33 11.045282073181728 1107 34-35 10.694826694643172 1107 36-37 11.154905328581972 1107 38-39 10.76284367446032 1107 40-41 10.342091880604883 1107 42-43 10.297662603087968 1107 44-45 9.937308099702836 1107 46-47 10.252404741128974 1107 48-49 10.423011273378457 1107 50-51 11.903059757438088 1107 52-53 10.849855365141654 1107 54-55 10.624378732684136 1107 56-57 10.494887079030278 1107 58-59 10.591859260174477 1107 60-61 10.454818756967008 1107 62-63 10.29932495311084 1107 64-65 10.492941079823265 1107 66-67 10.438614112705924 1107 68-69 10.098115752646198 1107 70-71 9.853464872148276 1107 72-73 9.466359710098104 1107 74-75 9.450777079101664 1107 76-77 9.011698511012275 1107 78-79 8.667850384751105 1107 80-81 8.685122109394412 1107 82-83 8.823038754361603 1107 84-85 8.622330013466378 1107 86-87 8.146995681745741 1107 88-89 8.052606536162171 1107 90-91 7.725194032744051 1107 92-93 7.192009722392333 1107 94-95 6.976576573050474 1107 96-97 6.653951402913547 1107 98-99 6.277073302309747 1107 100-101 5.655634334130223 1108 1 6.940573933238269 1108 2 8.015138531986114 1108 3 7.8954596845027325 1108 4 9.229304361879606 1108 5 10.981609157805497 1108 6 9.423286581043516 1108 7 8.611636355194786 1108 8 5.870147989771894 1108 9 7.135517951089739 1108 10-11 6.116778502008106 1108 12-13 5.3279390596521665 1108 14-15 7.451138551360657 1108 16-17 8.72746506585165 1108 18-19 8.506358773243054 1108 20-21 8.561427421763547 1108 22-23 9.493959893296523 1108 24-25 9.077161855303036 1108 26-27 7.521771008719416 1108 28-29 9.382126724628844 1108 30-31 9.947116273561939 1108 32-33 10.222273819130667 1108 34-35 9.723123424240793 1108 36-37 9.504410071643587 1108 38-39 9.277175185122324 1108 40-41 9.426018125545824 1108 42-43 8.702519714627964 1108 44-45 8.147148166190334 1108 46-47 8.912683039592906 1108 48-49 9.421926619753073 1108 50-51 11.035653546850869 1108 52-53 10.318419140832368 1108 54-55 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-14.692949481226396 2313 68-69 -14.246399999979243 2313 70-71 -13.894784836136438 2313 72-73 -13.510303067031415 2313 74-75 -13.057974379474766 2313 76-77 -12.662455514658669 2313 78-79 -12.287517176509105 2313 80-81 -11.877304961784118 2313 82-83 -11.411208620200826 2313 84-85 -11.001065552461277 2313 86-87 -10.530367212069892 2313 88-89 -10.107545155786484 2313 90-91 -9.639450074607174 2313 92-93 -9.119833918214434 2313 94-95 -8.535675468956528 2313 96-97 -7.905850230692055 2313 98-99 -7.241013045414874 2313 100-101 -6.345240811727418 2314 1 -18.675359479365774 2314 2 -17.396276450177975 2314 3 -18.044492753071584 2314 4 -19.49304997819174 2314 5 -18.80435992661781 2314 6 -19.81512007769608 2314 7 -20.02332202768274 2314 8 -20.4835975512507 2314 9 -21.75330487887459 2314 10-11 -22.126978929620908 2314 12-13 -22.04393965616234 2314 14-15 -21.957303779198202 2314 16-17 -20.75113184259068 2314 18-19 -19.691917088825914 2314 20-21 -19.339583279782232 2314 22-23 -18.99890574285092 2314 24-25 -18.835144922342625 2314 26-27 -18.5656249484744 2314 28-29 -18.372332252779003 2314 30-31 -18.351040682442818 2314 32-33 -18.226596573259346 2314 34-35 -17.886270154831738 2314 36-37 -17.403437728594223 2314 38-39 -17.287331354711206 2314 40-41 -17.1474063691034 2314 42-43 -17.013889322232917 2314 44-45 -16.62366039504629 2314 46-47 -16.26597332188153 2314 48-49 -16.004351620437177 2314 50-51 -13.863276298571249 2314 52-53 -15.700903094601639 2314 54-55 -16.436006331493225 2314 56-57 -16.40388771676902 2314 58-59 -16.11525859280102 2314 60-61 -15.8211439034764 2314 62-63 -15.405167462291729 2314 64-65 -15.057233949348262 2314 66-67 -14.692949481226396 2314 68-69 -14.246399999979243 2314 70-71 -13.894784836136438 2314 72-73 -13.510303067031415 2314 74-75 -13.057974379474766 2314 76-77 -12.662455514658669 2314 78-79 -12.287517176509105 2314 80-81 -11.877304961784118 2314 82-83 -11.411208620200826 2314 84-85 -11.001065552461277 2314 86-87 -10.530367212069892 2314 88-89 -10.107545155786484 2314 90-91 -9.639450074607174 2314 92-93 -9.119833918214434 2314 94-95 -8.535675468956528 2314 96-97 -7.905850230692055 2314 98-99 -7.241013045414874 2314 100-101 -6.345240811727418 2315 1 -18.675359479365774 2315 2 -17.396276450177975 2315 3 -18.044492753071584 2315 4 -19.49304997819174 2315 5 -18.80435992661781 2315 6 -19.81512007769608 2315 7 -20.02332202768274 2315 8 -20.4835975512507 2315 9 -21.75330487887459 2315 10-11 -22.126978929620908 2315 12-13 -22.04393965616234 2315 14-15 -21.957303779198202 2315 16-17 -20.75113184259068 2315 18-19 -19.691917088825914 2315 20-21 -19.339583279782232 2315 22-23 -18.99890574285092 2315 24-25 -18.835144922342625 2315 26-27 -18.5656249484744 2315 28-29 -18.372332252779003 2315 30-31 -18.351040682442818 2315 32-33 -18.226596573259346 2315 34-35 -17.886270154831738 2315 36-37 -17.403437728594223 2315 38-39 -17.287331354711206 2315 40-41 -17.1474063691034 2315 42-43 -17.013889322232917 2315 44-45 -16.62366039504629 2315 46-47 -16.26597332188153 2315 48-49 -16.004351620437177 2315 50-51 -13.863276298571249 2315 52-53 -15.700903094601639 2315 54-55 -16.436006331493225 2315 56-57 -16.40388771676902 2315 58-59 -16.11525859280102 2315 60-61 -15.8211439034764 2315 62-63 -15.405167462291729 2315 64-65 -15.057233949348262 2315 66-67 -14.692949481226396 2315 68-69 -14.246399999979243 2315 70-71 -13.894784836136438 2315 72-73 -13.510303067031415 2315 74-75 -13.057974379474766 2315 76-77 -12.662455514658669 2315 78-79 -12.287517176509105 2315 80-81 -11.877304961784118 2315 82-83 -11.411208620200826 2315 84-85 -11.001065552461277 2315 86-87 -10.530367212069892 2315 88-89 -10.107545155786484 2315 90-91 -9.639450074607174 2315 92-93 -9.119833918214434 2315 94-95 -8.535675468956528 2315 96-97 -7.905850230692055 2315 98-99 -7.241013045414874 2315 100-101 -6.345240811727418 2316 1 -18.675359479365774 2316 2 -17.396276450177975 2316 3 -18.044492753071584 2316 4 -19.49304997819174 2316 5 -18.80435992661781 2316 6 -19.81512007769608 2316 7 -20.02332202768274 2316 8 -20.4835975512507 2316 9 -21.75330487887459 2316 10-11 -22.126978929620908 2316 12-13 -22.04393965616234 2316 14-15 -21.957303779198202 2316 16-17 -20.75113184259068 2316 18-19 -19.691917088825914 2316 20-21 -19.339583279782232 2316 22-23 -18.99890574285092 2316 24-25 -18.835144922342625 2316 26-27 -18.5656249484744 2316 28-29 -18.372332252779003 2316 30-31 -18.351040682442818 2316 32-33 -18.226596573259346 2316 34-35 -17.886270154831738 2316 36-37 -17.403437728594223 2316 38-39 -17.287331354711206 2316 40-41 -17.1474063691034 2316 42-43 -17.013889322232917 2316 44-45 -16.62366039504629 2316 46-47 -16.26597332188153 2316 48-49 -16.004351620437177 2316 50-51 -13.863276298571249 2316 52-53 -15.700903094601639 2316 54-55 -16.436006331493225 2316 56-57 -16.40388771676902 2316 58-59 -16.11525859280102 2316 60-61 -15.8211439034764 2316 62-63 -15.405167462291729 2316 64-65 -15.057233949348262 2316 66-67 -14.692949481226396 2316 68-69 -14.246399999979243 2316 70-71 -13.894784836136438 2316 72-73 -13.510303067031415 2316 74-75 -13.057974379474766 2316 76-77 -12.662455514658669 2316 78-79 -12.287517176509105 2316 80-81 -11.877304961784118 2316 82-83 -11.411208620200826 2316 84-85 -11.001065552461277 2316 86-87 -10.530367212069892 2316 88-89 -10.107545155786484 2316 90-91 -9.639450074607174 2316 92-93 -9.119833918214434 2316 94-95 -8.535675468956528 2316 96-97 -7.905850230692055 2316 98-99 -7.241013045414874 2316 100-101 -6.345240811727418 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 391420.0 3 16922.0 4 60014.0 5 32808.0 6 19086.0 7 18738.0 8 18577.0 9 18250.0 10 19074.0 11 20502.0 12 20873.0 13 21627.0 14 21972.0 15 22861.0 16 23492.0 17 23957.0 18 23806.0 19 24080.0 20 25455.0 21 26951.0 22 28486.0 23 30336.0 24 32769.0 25 34617.0 26 36934.0 27 39777.0 28 42863.0 29 46157.0 30 49714.0 31 53841.0 32 57902.0 33 62155.0 34 64955.0 35 60791.0 36 44917.0 37 18697.0 38 2236.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.749990372332626 20.86647367631864 14.417657223905127 22.965878727443602 2 14.755905653421097 20.957044070966756 42.04222755761778 22.24482271799437 3 18.444304862819678 26.41022267131093 30.64666290954862 24.498809556320772 4 12.151291635861975 17.547553289490864 40.07551614556083 30.225638929086323 5 12.488816006728742 38.087200807599565 36.193424987981025 13.230558197690664 6 29.11701509181221 39.17029031084577 18.330653139906097 13.382041457435923 7 25.611021781666913 33.25564745589929 23.56841415179597 17.564916610637823 8 23.991323556384923 37.354482164645084 20.96995940891728 17.684234870052713 9 24.96417317783369 17.182232215797434 21.862733798556487 35.99086080781239 10-11 23.60339020828965 27.554981328165283 29.48521647805941 19.356411985485646 12-13 24.290508515864783 24.97399432754816 29.821186887542773 20.914310269044282 14-15 21.540978538221054 26.757541546009485 27.33403353246488 24.367446383304582 16-17 20.995782561157416 29.862754181731166 28.024502297090354 21.116960960021064 18-19 20.785638336597888 28.70710937912111 30.17286816061703 20.33438412366397 20-21 21.360854692424603 27.910567538365665 30.389865994772496 20.338711774437236 22-23 21.113158877871367 27.849390172681126 30.05950709154409 20.977943857903412 24-25 21.006720825467895 27.986029494358082 30.292404204088964 20.71484547608506 26-27 21.00655899192551 28.114830275219134 30.081591809171893 20.797018923683467 28-29 20.95164902031851 28.247835945660405 30.19042480036869 20.610090233652397 30-31 21.13461160585837 28.0187039025383 30.120458676323008 20.726225815280323 32-33 20.989508621906108 28.177691169634127 30.022837078481462 20.8099631299783 34-35 21.168251905240147 28.2333803137844 29.988268953236165 20.610098827739286 36-37 21.181559673159857 28.103636563869888 29.88390005602949 20.830903706940767 38-39 21.138390060899624 28.192768966024996 29.985824518528126 20.683016454547253 40-41 21.251203332057102 28.282634914758876 29.829238834895655 20.636922918288363 42-43 21.17601496457199 28.227077804831342 29.627612644652967 20.9692945859437 44-45 21.221612661922777 28.43462139914062 29.527541010765628 20.81622492817098 46-47 21.33650962544686 28.399808965958012 29.21784904096697 21.045832367628158 48-49 21.311753129208107 28.499356039899492 29.130383223403538 21.05850760748886 50-51 21.4158027730695 28.75730253213827 28.882845072755885 20.944049622036346 52-53 21.05391005217656 28.924763766019684 28.959850753531907 21.06147542827185 54-55 21.034791668654883 28.787057095946068 29.0304149614673 21.14773627393175 56-57 20.98504568001474 28.914912017357725 29.099900725285373 21.000141577342166 58-59 20.99490401419747 28.808748911411953 29.167655652253238 21.028691422137342 60-61 20.96976642195946 28.948235100389326 29.132815689938212 20.949182787713 62-63 20.970156819341472 28.91618958408565 29.10487214912961 21.008781447443276 64-65 20.81732592119213 28.762883036707866 29.17288663092192 21.24690441117809 66-67 20.817269188504593 28.861447914920564 28.95891924779448 21.362363648780367 68-69 20.894103723273847 28.828183574948063 28.963341420452377 21.314371281325712 70-71 20.929836708313257 28.689604360146237 28.950765804954504 21.429793126586006 72-73 20.952705803351172 28.83340270132932 28.831432486058674 21.38245900926083 74-75 20.910597163729374 29.024862730679757 28.63154726714045 21.432992838450417 76-77 20.978842295816012 28.900685120217474 28.715333263943737 21.405139320022776 78-79 20.845240608000477 28.911865114069705 28.697806586174096 21.54508769175572 80-81 20.89828362379794 29.02213451113676 28.44893248277021 21.63064938229509 82-83 21.01872404144444 28.860542334447842 28.579326370834856 21.541407253272865 84-85 21.07084933573774 28.89831304284161 28.441001064793227 21.58983655662742 86-87 21.021480410506403 28.95623414519403 28.468996059998336 21.553289384301234 88-89 21.099058557499635 28.857963241044143 28.384243511690872 21.658734689765353 90-91 21.016570786584275 28.933012602328283 28.457105741898577 21.593310869188862 92-93 21.05361304605336 28.831033631442587 28.393254496063474 21.72209882644058 94-95 20.92095253593012 28.801289949186003 28.366657555044483 21.91109995983939 96-97 20.93919655555986 28.813111768003107 28.26962104675625 21.978070629680786 98-99 20.82575058873743 28.861609533407684 28.078548538130534 22.234091339724348 100-101 21.087374253875282 28.880880019580204 28.054970379334392 21.976775347210122 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 90217.0 1 82060.0 2 54898.5 3 28103.0 4 17528.0 5 13832.5 6 12827.5 7 13008.5 8 13382.0 9 13261.5 10 12199.0 11 10350.5 12 8286.0 13 6372.5 14 4917.5 15 3998.0 16 3687.0 17 3939.0 18 4532.5 19 5285.0 20 6193.5 21 7201.5 22 8237.5 23 9420.5 24 10850.0 25 12375.5 26 14136.0 27 16135.5 28 18448.5 29 21027.0 30 23592.5 31 26332.5 32 29385.0 33 32279.5 34 34629.5 35 36912.0 36 39472.0 37 41967.0 38 43696.5 39 44672.0 40 45090.0 41 45299.5 42 45904.0 43 46206.5 44 46323.5 45 46561.0 46 45927.0 47 44984.0 48 43859.5 49 41878.0 50 39172.0 51 36145.5 52 33181.5 53 30254.0 54 26931.0 55 23070.0 56 19180.5 57 15562.5 58 12357.5 59 9808.5 60 7601.0 61 5584.5 62 4052.5 63 2852.0 64 1902.0 65 1216.5 66 765.5 67 494.0 68 316.0 69 196.5 70 126.0 71 82.0 72 59.0 73 47.0 74 30.5 75 19.0 76 14.5 77 10.5 78 8.0 79 4.0 80 2.0 81 2.5 82 1.5 83 1.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.647466532445485 2 28.60490438024997 3 22.42223432038972 4 28.706276466524745 5 31.76143469429158 6 24.466432293204797 7 23.419584136905602 8 19.494898653593797 9 11.432447285231133 10-11 15.029936544540801 12-13 15.138466951030669 14-15 19.22246518424168 16-17 22.463932338144925 18-19 26.367881162896406 20-21 28.40042680280529 22-23 27.996479262057438 24-25 26.556180294653153 26-27 27.983928126613026 28-29 24.984238855490528 30-31 26.813334120855558 32-33 25.839866771783765 34-35 23.92984717788843 36-37 26.838853692922825 38-39 26.970528264771822 40-41 24.040078889182784 42-43 24.905304732580564 44-45 23.659018713068846 46-47 26.871884686202353 48-49 22.311970509004716 50-51 27.920723048121886 52-53 21.50401122732771 54-55 22.637786429108875 56-57 24.72503062348648 58-59 23.995138762271864 60-61 23.584707003212575 62-63 23.374579809813383 64-65 21.94265896606892 66-67 22.4431314794033 68-69 23.406005798560376 70-71 21.925774318386676 72-73 21.795126949438508 74-75 19.153064788254962 76-77 21.356864411785097 78-79 20.461528683357002 80-81 21.48609937674464 82-83 22.31123220691975 84-85 19.327207780034772 86-87 20.84968297950473 88-89 19.709070458414974 90-91 20.82676351478017 92-93 20.816491485771955 94-95 19.910851628245 96-97 18.794538875777786 98-99 18.173691022503448 100-101 19.865365799793018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1557628.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.11896296130057 #Duplication Level Percentage of deduplicated Percentage of total 1 91.28279292538949 74.9604829124413 2 6.083789566511755 9.991889801534516 3 1.3593149335965216 3.348765980542665 4 0.5070333699927702 1.665482181223188 5 0.2538641893287378 1.0423531980343606 6 0.14701132236048334 0.7243450401487409 7 0.08794234839290188 0.5055214115284551 8 0.06495660871514172 0.4267335476136334 9 0.03969269388594245 0.2933570573148555 >10 0.16762462485228993 2.157095424156013 >50 0.004573038636225101 0.2583095951917732 >100 0.0010923122084752661 0.14223540202619153 >500 7.801653231397969E-5 0.03876015220113788 >1k 1.5603306462795937E-4 0.15760326349553586 >5k 0.0 0.0 >10k+ 7.801653231397969E-5 4.287065032547674 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 66916 4.296019331958593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 9.630027195196798E-5 0.0 28-29 0.0 0.0 0.0 6.099017223624639E-4 0.0 30-31 0.0 3.2100090650655996E-5 0.0 0.001540804351231488 0.0 32-33 0.0 6.420018130131199E-5 0.0 0.003884110968729376 0.0 34-35 0.0 6.420018130131199E-5 0.0 0.006869419399240384 0.0 36-37 0.0 6.420018130131199E-5 0.0 0.011523932543585504 0.0 38-39 0.0 6.420018130131199E-5 0.0 0.02006255665666 0.0 40-41 0.0 6.420018130131199E-5 0.0 0.029403683036000897 0.0 42-43 0.0 6.420018130131199E-5 0.0 0.03832750823688326 0.0 44-45 0.0 6.420018130131199E-5 0.0 0.04914523878615433 0.0 46-47 0.0 6.420018130131199E-5 0.0 0.0628198774033338 0.0 48-49 0.0 6.420018130131199E-5 0.0 0.0773291183774303 0.0 50-51 0.0 6.420018130131199E-5 0.0 0.09405326560642208 0.0 52-53 0.0 6.420018130131199E-5 0.0 0.11170831546428286 0.0 54-55 0.0 6.420018130131199E-5 0.0 0.13331167647217435 0.0 56-57 0.0 6.420018130131199E-5 0.0 0.15879914844879522 0.0 58-59 0.0 6.420018130131199E-5 0.0 0.18765712994373496 0.0 60-61 6.420018130131199E-5 6.420018130131199E-5 0.0 0.22017452177284946 0.0 62-63 6.420018130131199E-5 6.420018130131199E-5 0.0 0.2524030127861081 0.0 64-65 6.420018130131199E-5 6.420018130131199E-5 0.0 0.2846636038900174 0.0 66-67 6.420018130131199E-5 6.420018130131199E-5 0.0 0.32305531230820195 0.0 68-69 6.420018130131199E-5 6.420018130131199E-5 0.0 0.36924734275449594 0.0 70-71 6.420018130131199E-5 6.420018130131199E-5 0.0 0.4196444850760258 0.0 72-73 6.420018130131199E-5 6.420018130131199E-5 0.0 0.4699453271256038 0.0 74-75 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.5216585731638106 0.0 76-77 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.5798560375134498 0.0 78-79 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.6486786318684564 0.0 80-81 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.7244990459853059 0.0 82-83 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.8074777803172516 0.0 84-85 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.8930245219012498 0.0 86-87 1.2840036260262398E-4 6.420018130131199E-5 0.0 0.9809787702840473 0.0 88-89 1.2840036260262398E-4 6.420018130131199E-5 0.0 1.0762518393351943 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1440 0.0 53.974216 1 GTATCAA 2610 0.0 41.92552 1 ATCAACG 2885 0.0 35.727886 3 TCAACGC 3000 0.0 34.189087 4 CAACGCA 2985 0.0 33.849133 5 TATCAAC 3135 0.0 33.26539 2 AACGCAG 3250 0.0 30.391191 6 CGCGCGA 35 0.006450497 28.64704 18-19 ACGCAGA 3710 0.0 26.621675 7 GGACCGT 125 8.278601E-6 26.214205 6 CGCAGAG 3850 0.0 25.866241 8 GCAGAGT 4235 0.0 21.632887 9 GTACATG 2360 0.0 21.450056 1 GAGTACT 2740 0.0 20.678871 12-13 GTACTAG 280 1.4964826E-8 20.088146 1 CAGAGTA 3985 0.0 19.678835 10-11 AGAGTAC 3630 0.0 19.64515 10-11 TACATGG 2405 0.0 19.55567 2 GTGTAGC 585 0.0 19.229681 1 GTATAAC 355 5.9299055E-10 18.724932 1 >>END_MODULE