FastQCFastQC Report
Sat 18 Jun 2016
SRR1931021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1557628
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.2695123611029077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39200.251664710701143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24000.15408043512314878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.054.602071
GTATCAA41750.041.6584471
ATCAACG50900.033.5151523
CAACGCA51100.033.332545
TCAACGC51650.033.028484
AACGCAG53550.032.072296
TATCAAC53700.031.8571322
ACGCAGA61750.027.1218057
CGCAGAG61700.026.9899968
GTGGTAT10350.024.3340261
GCAGAGT70350.023.0644389
GTACATG36450.021.3808421
AGAGTAC64850.020.96020310-11
CAGAGTA67650.020.9342510-11
GAGTACT51800.020.92846312-13
TACATGG37200.020.0555782
ACATGGG36500.020.0489853
TCTAACG1951.0292415E-519.4954762
TGGTATC12000.019.008092
GTACTTT58350.018.49785414-15
Produced by FastQC (version 0.11.5)