FastQCFastQC Report
Sat 18 Jun 2016
SRR1931020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106675
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN922794.3803149512857935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.056.832551
GTATCAA33550.039.2427441
ATCAACG39050.033.0284543
TCAACGC41350.031.7959964
CAACGCA42450.031.2060765
TATCAAC42100.030.9057252
AACGCAG45050.029.610576
GTACATG33750.025.0454541
ACGCAGA50600.024.9815797
CGCAGAG51050.024.5104798
TACATGG34500.024.0537762
ACATGGG34500.023.0731723
GTGGTAT10800.022.294251
GCAGAGT55200.022.1022959
GAGTACT36100.020.6889212-13
CAGAGTA53500.020.67975410-11
GTGTAGC7600.020.2221091
AGAGTAC49600.019.62724310-11
TGGTATC11000.018.6275022
GTACTTT42250.018.45642714-15
Produced by FastQC (version 0.11.5)