##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931020_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2106675 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.92572513558095 28.0 2.0 31.0 2.0 33.0 2 19.661752762053947 27.0 2.0 31.0 2.0 33.0 3 20.299370809450913 27.0 2.0 31.0 2.0 33.0 4 21.81037321846037 30.0 2.0 35.0 2.0 37.0 5 21.161362336383164 30.0 2.0 35.0 2.0 37.0 6 22.118099374606903 30.0 2.0 35.0 2.0 37.0 7 22.292860075711726 30.0 2.0 35.0 2.0 37.0 8 22.71561346671888 30.0 2.0 35.0 2.0 37.0 9 24.00635076601754 32.0 2.0 37.0 2.0 39.0 10-11 24.349402494452157 32.0 2.0 37.0 2.0 39.0 12-13 24.236530551698767 32.0 2.0 37.0 2.0 39.0 14-15 24.172220442165973 32.0 2.0 38.0 2.0 40.0 16-17 23.005731069101785 31.0 2.0 38.0 2.0 40.0 18-19 21.979957990672503 28.5 2.0 37.0 2.0 40.0 20-21 21.640102293899155 27.0 2.0 37.0 2.0 40.0 22-23 21.26989402731793 27.0 2.0 37.0 2.0 39.5 24-25 21.095892104857178 27.0 2.0 37.0 2.0 39.0 26-27 20.809878362822932 26.5 2.0 37.0 2.0 39.0 28-29 20.607847674653186 25.0 2.0 36.0 2.0 39.0 30-31 20.579995253183334 25.0 2.0 36.0 2.0 39.0 32-33 20.43020423178706 25.0 2.0 36.0 2.0 39.0 34-35 20.102177839486394 24.5 2.0 36.0 2.0 39.0 36-37 19.643719130857868 24.0 2.0 36.0 2.0 39.0 38-39 19.51410540306407 24.0 2.0 35.0 2.0 39.0 40-41 19.371793466006856 23.5 2.0 35.0 2.0 39.0 42-43 19.21478823264148 23.5 2.0 35.0 2.0 38.5 44-45 18.815381822065575 20.5 2.0 35.0 2.0 38.0 46-47 18.48194049865309 18.5 2.0 35.0 2.0 38.0 48-49 18.206786523787486 16.5 2.0 34.5 2.0 38.0 50-51 16.03219504669681 10.0 2.0 32.5 2.0 37.5 52-53 17.899143199591773 17.0 2.0 33.5 2.0 38.0 54-55 18.627870459373185 20.0 2.0 35.0 2.0 39.0 56-57 18.592454222886776 19.5 2.0 35.0 2.0 39.0 58-59 18.312200505535976 17.5 2.0 35.0 2.0 39.0 60-61 18.02002444610583 14.5 2.0 35.0 2.0 39.0 62-63 17.581314156194004 11.0 2.0 34.0 2.0 38.0 64-65 17.221923647453927 8.0 2.0 34.0 2.0 38.0 66-67 16.85903022535512 7.5 2.0 34.0 2.0 38.0 68-69 16.41545729645057 4.5 2.0 33.0 2.0 37.5 70-71 16.060325394282458 2.0 2.0 32.5 2.0 37.0 72-73 15.641912017753095 2.0 2.0 32.0 2.0 36.0 74-75 15.189227574258013 2.0 2.0 31.0 2.0 36.0 76-77 14.769347194037998 2.0 2.0 31.0 2.0 35.5 78-79 14.38403028469033 2.0 2.0 31.0 2.0 35.0 80-81 13.93891013089347 2.0 2.0 30.0 2.0 34.5 82-83 13.47885696654681 2.0 2.0 30.0 2.0 34.0 84-85 13.065786369515944 2.0 2.0 29.0 2.0 34.0 86-87 12.582315734510544 2.0 2.0 29.0 2.0 34.0 88-89 12.156210141573807 2.0 2.0 29.0 2.0 34.0 90-91 11.668128923540651 2.0 2.0 27.5 2.0 34.0 92-93 11.125694993295122 2.0 2.0 26.0 2.0 34.0 94-95 10.565810103599274 2.0 2.0 25.0 2.0 33.0 96-97 9.930440385916196 2.0 2.0 24.0 2.0 32.5 98-99 9.265129172748527 2.0 2.0 18.5 2.0 32.0 100-101 8.370344025537873 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.779775157912283 1101 2 9.455581594291424 1101 3 8.649824064826845 1101 4 10.734740899434069 1101 5 11.60841478495066 1101 6 10.805353671736501 1101 7 10.273034363580852 1101 8 9.734205433357214 1101 9 9.695008091996659 1101 10-11 9.127435887091735 1101 12-13 8.86966189370688 1101 14-15 9.491229115740026 1101 16-17 10.624601132409087 1101 18-19 11.570055526855896 1101 20-21 11.631724266127662 1101 22-23 11.699975619629576 1101 24-25 11.66263748223177 1101 26-27 11.585989070375817 1101 28-29 11.543391205700491 1101 30-31 11.276678561766598 1101 32-33 11.027908234421279 1101 34-35 11.103903916327106 1101 36-37 11.34654649049888 1101 38-39 11.091477985519145 1101 40-41 10.865081457538938 1101 42-43 10.500021949457437 1101 44-45 10.61691086364765 1101 46-47 10.42383827842503 1101 48-49 10.502182538026396 1101 50-51 11.88966312579071 1101 52-53 11.181016857777664 1101 54-55 11.055306075898162 1101 56-57 11.06583213136658 1101 58-59 10.804047609947851 1101 60-61 10.572956922509174 1101 62-63 10.581534742687882 1101 64-65 10.365807063036417 1101 66-67 10.028876336235406 1101 68-69 10.058260124537934 1101 70-71 9.777646606912535 1101 72-73 9.541140104694266 1101 74-75 9.279450811553454 1101 76-77 9.084983288912724 1101 78-79 8.717997985555591 1101 80-81 8.637149094885098 1101 82-83 8.141864472460554 1101 84-85 7.893453074731646 1101 86-87 7.800491710085431 1101 88-89 7.356730803662698 1101 90-91 7.281322997702668 1101 92-93 7.110232753282077 1101 94-95 6.874058141639928 1101 96-97 6.571841863035269 1101 98-99 6.178093115738342 1101 100-101 5.5612308250915365 1102 1 7.576923234931421 1102 2 8.833287621122924 1102 3 8.355399205462124 1102 4 10.317239525762439 1102 5 11.028246883485167 1102 6 10.112247324576469 1102 7 9.713244840895872 1102 8 9.114571016001232 1102 9 9.098886469182982 1102 10-11 8.16937320515332 1102 12-13 8.360910144069027 1102 14-15 8.993760285066706 1102 16-17 9.785892500649798 1102 18-19 10.94108598782502 1102 20-21 10.85969795965169 1102 22-23 11.075961713927747 1102 24-25 10.782153503441048 1102 26-27 10.929038504486876 1102 28-29 10.653294501343321 1102 30-31 10.679162648963128 1102 32-33 10.867728344548823 1102 34-35 10.567480734063995 1102 36-37 10.803141761667504 1102 38-39 10.554954262183522 1102 40-41 10.359537669563693 1102 42-43 9.876756256388287 1102 44-45 9.660150007921963 1102 46-47 9.80005616984705 1102 48-49 9.425768134508097 1102 50-51 11.1771438492188 1102 52-53 10.416109730733499 1102 54-55 9.974610812062867 1102 56-57 9.925860359388633 1102 58-59 10.025008510523783 1102 60-61 9.887394025353334 1102 62-63 9.81232571184643 1102 64-65 9.43420538848553 1102 66-67 9.273296147290463 1102 68-69 9.250612307373892 1102 70-71 8.778536065935674 1102 72-73 8.717685764263896 1102 74-75 8.24342129116413 1102 76-77 8.03373996995202 1102 78-79 7.74404800739617 1102 80-81 6.975790238715158 1102 82-83 6.977762843078754 1102 84-85 6.860374849313462 1102 86-87 6.466198595660977 1102 88-89 6.214963321366325 1102 90-91 6.180456920324298 1102 92-93 5.810159207381254 1102 94-95 5.395128829666081 1102 96-97 5.316003944596824 1102 98-99 4.979036671385062 1102 100-101 4.661737250261238 1103 1 7.864272350261043 1103 2 9.145462512960783 1103 3 8.475377361955427 1103 4 10.54278313691291 1103 5 11.346602827314001 1103 6 10.462912514946435 1103 7 10.269138052647893 1103 8 9.768649870809629 1103 9 9.556510442368886 1103 10-11 8.975517307928982 1103 12-13 9.025733111768144 1103 14-15 9.646060436416377 1103 16-17 10.397619257858768 1103 18-19 11.238409301400559 1103 20-21 11.661061966685416 1103 22-23 11.85700093568699 1103 24-25 11.90586566236615 1103 26-27 11.915576806068543 1103 28-29 11.540628653076695 1103 30-31 11.187647175225287 1103 32-33 11.363448463438356 1103 34-35 11.231630966898386 1103 36-37 11.438605777353366 1103 38-39 11.253317002327297 1103 40-41 10.707229158663047 1103 42-43 10.381397688078426 1103 44-45 10.728171674206184 1103 46-47 10.411479893333166 1103 48-49 10.217850427599807 1103 50-51 12.060175026582142 1103 52-53 11.217614387212379 1103 54-55 10.719845136546365 1103 56-57 10.631773863950649 1103 58-59 10.659234790193398 1103 60-61 10.50833144032103 1103 62-63 10.392463711313926 1103 64-65 9.892844629137358 1103 66-67 9.645111419604927 1103 68-69 9.564607073459026 1103 70-71 9.52668233550695 1103 72-73 9.26431492162534 1103 74-75 9.01410626859125 1103 76-77 8.60408444196905 1103 78-79 8.210104468639408 1103 80-81 7.7006474262361895 1103 82-83 7.662616339858026 1103 84-85 7.3438661990591 1103 86-87 7.076121779747069 1103 88-89 6.9212887428026395 1103 90-91 6.691817236616272 1103 92-93 6.224406619995733 1103 94-95 5.737029553310613 1103 96-97 5.497555732224104 1103 98-99 4.998350995935434 1103 100-101 4.35731490150328 1104 1 7.778782292521324 1104 2 9.238820418111942 1104 3 8.850778782741724 1104 4 10.766644561591772 1104 5 11.419415692241984 1104 6 10.745699267249538 1104 7 10.244312407130831 1104 8 9.76038181903851 1104 9 9.556508009515635 1104 10-11 8.817320619853206 1104 12-13 8.7328696840401 1104 14-15 9.189472269814587 1104 16-17 10.404119030792462 1104 18-19 11.108793773309216 1104 20-21 11.341016269591869 1104 22-23 11.578760983370913 1104 24-25 11.371712457487465 1104 26-27 11.402952325077237 1104 28-29 11.321543527541468 1104 30-31 11.088472317952672 1104 32-33 11.14047421924477 1104 34-35 11.130992748395727 1104 36-37 11.145576307390996 1104 38-39 10.67496270590846 1104 40-41 10.40255362019153 1104 42-43 10.015745926692674 1104 44-45 10.063082712872326 1104 46-47 9.955540893073664 1104 48-49 10.093888039511054 1104 50-51 11.694025341231159 1104 52-53 10.8046363315487 1104 54-55 10.555205036637194 1104 56-57 10.212963853570475 1104 58-59 10.442195086028352 1104 60-61 10.325238553983933 1104 62-63 9.892899597521271 1104 64-65 9.598743284580461 1104 66-67 9.570746799778792 1104 68-69 9.29252893021263 1104 70-71 8.940897699786175 1104 72-73 8.780622742437584 1104 74-75 8.486682335802877 1104 76-77 8.269141776210645 1104 78-79 7.9489690965750555 1104 80-81 7.535281930582888 1104 82-83 7.436955820200573 1104 84-85 7.213714024088297 1104 86-87 6.843437004542707 1104 88-89 6.451147353481241 1104 90-91 5.92397651551857 1104 92-93 5.583336205113156 1104 94-95 5.389571276743855 1104 96-97 5.035224410049457 1104 98-99 4.629475703993043 1104 100-101 3.8532528475263756 1105 1 7.771728076430556 1105 2 8.921371024094409 1105 3 8.663584997285305 1105 4 10.564233524974963 1105 5 10.340254397760294 1105 6 10.049367395150515 1105 7 9.673273856331452 1105 8 9.056873245031287 1105 9 8.960295399923059 1105 10-11 8.499255573437523 1105 12-13 8.10235056980596 1105 14-15 8.763144265688751 1105 16-17 9.965152424294264 1105 18-19 10.410996906367494 1105 20-21 10.897136531324719 1105 22-23 11.094346497811351 1105 24-25 11.060292266667453 1105 26-27 11.161872838228344 1105 28-29 10.9565688305327 1105 30-31 10.558548710423018 1105 32-33 10.419542037707895 1105 34-35 10.438570752521564 1105 36-37 10.354119693156854 1105 38-39 10.632589865526818 1105 40-41 10.128654380892922 1105 42-43 9.76775243778086 1105 44-45 9.750958228602084 1105 46-47 9.667272133403731 1105 48-49 9.49386386467867 1105 50-51 11.011587551133173 1105 52-53 10.054589593539415 1105 54-55 9.914659007756324 1105 56-57 9.678671048000592 1105 58-59 9.570295911195966 1105 60-61 9.39258480204995 1105 62-63 9.347974700667223 1105 64-65 8.95330185214106 1105 66-67 8.82464217110422 1105 68-69 8.567429007572091 1105 70-71 8.520374072916654 1105 72-73 8.485652719229746 1105 74-75 8.06806771486425 1105 76-77 7.5191304946222 1105 78-79 7.389954301577632 1105 80-81 7.107914408664373 1105 82-83 6.886802712928784 1105 84-85 6.54756926519196 1105 86-87 6.268656995517439 1105 88-89 5.952929038628222 1105 90-91 5.701715524028108 1105 92-93 5.572295559161638 1105 94-95 5.261192441003782 1105 96-97 4.896203007264516 1105 98-99 4.619765479650599 1105 100-101 4.241703724266964 1106 1 8.026722651580869 1106 2 9.161564316655262 1106 3 8.840580405537533 1106 4 10.383703084464251 1106 5 11.19249101807894 1106 6 10.40617396696842 1106 7 9.655580935114479 1106 8 9.206418268054609 1106 9 9.117613619022404 1106 10-11 8.814724306283054 1106 12-13 8.60069687864782 1106 14-15 9.130457447427673 1106 16-17 9.981768851901581 1106 18-19 11.19970627650369 1106 20-21 11.430414189985427 1106 22-23 11.495244100544433 1106 24-25 11.002183004382427 1106 26-27 11.362162809360395 1106 28-29 11.307770127975918 1106 30-31 10.931807834180631 1106 32-33 11.061644037798306 1106 34-35 10.9920983579025 1106 36-37 11.132877451014814 1106 38-39 10.972488181885968 1106 40-41 10.977765254487462 1106 42-43 10.453577353986308 1106 44-45 9.916640564904018 1106 46-47 9.873862034141897 1106 48-49 10.256406407770701 1106 50-51 11.684798884548309 1106 52-53 11.057796470828023 1106 54-55 10.553382585832182 1106 56-57 10.385333199340984 1106 58-59 10.048737380942743 1106 60-61 9.97913981260903 1106 62-63 10.088445693866785 1106 64-65 9.90402815232899 1106 66-67 9.570123077570766 1106 68-69 9.275622145137238 1106 70-71 8.978212819259726 1106 72-73 8.917760967584533 1106 74-75 8.526362568361018 1106 76-77 8.331826257427103 1106 78-79 7.911120498951746 1106 80-81 7.607244778781203 1106 82-83 7.418050089303563 1106 84-85 7.212470972082162 1106 86-87 6.931487653022138 1106 88-89 6.66186203850552 1106 90-91 6.32946023964328 1106 92-93 5.976973200385327 1106 94-95 5.734235297316303 1106 96-97 5.207103403149381 1106 98-99 4.837695899495216 1106 100-101 4.362630243386329 1107 1 7.188924395137018 1107 2 8.099717047890614 1107 3 8.023502625800074 1107 4 9.7385006984127 1107 5 10.70164657755386 1107 6 9.606363251152956 1107 7 9.471965827653971 1107 8 8.173365973968085 1107 9 5.420479337483439 1107 10-11 7.371212722468897 1107 12-13 7.5133745884666645 1107 14-15 7.740817843255229 1107 16-17 8.840738749102318 1107 18-19 10.343942163651263 1107 20-21 10.597992501986234 1107 22-23 9.861529469286406 1107 24-25 10.165789419258086 1107 26-27 10.29672118825631 1107 28-29 10.502387969191364 1107 30-31 10.076990929622696 1107 32-33 10.4215544506331 1107 34-35 10.616266893264942 1107 36-37 10.882661317451358 1107 38-39 10.390571986592562 1107 40-41 10.171309907917884 1107 42-43 9.836199900535732 1107 44-45 9.0483631454679 1107 46-47 9.439067050015314 1107 48-49 9.56720292884707 1107 50-51 10.938542443846854 1107 52-53 9.660037488490346 1107 54-55 9.648118769031559 1107 56-57 9.346615765039285 1107 58-59 9.408379693674227 1107 60-61 9.026829348752546 1107 62-63 9.027834205167146 1107 64-65 9.176236368081465 1107 66-67 9.142999063360282 1107 68-69 8.756626012707596 1107 70-71 8.510883238015554 1107 72-73 8.28342706839332 1107 74-75 8.343064378056399 1107 76-77 7.967073114238815 1107 78-79 7.512805238426161 1107 80-81 7.1667645764581405 1107 82-83 7.25217840571365 1107 84-85 6.86104488424926 1107 86-87 6.601777673556796 1107 88-89 6.330212368571784 1107 90-91 5.902720856564846 1107 92-93 5.652271693040724 1107 94-95 5.540165466884698 1107 96-97 5.1102369855021745 1107 98-99 4.749791347856222 1107 100-101 4.337080815333218 1108 1 6.728327856262517 1108 2 7.87901700683712 1108 3 7.881716482933911 1108 4 9.3011096480748 1108 5 11.04968074669426 1108 6 9.470162752195083 1108 7 8.802380152166066 1108 8 5.877800541011602 1108 9 7.136588887158169 1108 10-11 5.9525164078096395 1108 12-13 4.884059826576937 1108 14-15 7.439744531185504 1108 16-17 8.47578382899874 1108 18-19 8.039040810464883 1108 20-21 8.21163706555599 1108 22-23 9.02400341610634 1108 24-25 9.05403548759637 1108 26-27 7.297901476262375 1108 28-29 9.120649669437686 1108 30-31 9.548560002129484 1108 32-33 10.088384212838616 1108 34-35 9.663539993751272 1108 36-37 9.137286349030973 1108 38-39 9.100598316094235 1108 40-41 9.585819555489412 1108 42-43 8.884457890177618 1108 44-45 8.351709794871045 1108 46-47 9.121422139069821 1108 48-49 9.272689689094655 1108 50-51 10.897242429636028 1108 52-53 10.09159381072871 1108 54-55 9.372721852780689 1108 56-57 9.45973275171269 1108 58-59 9.277316802876523 1108 60-61 9.290174892869263 1108 62-63 9.397766269526397 1108 64-65 8.899547056201218 1108 66-67 8.456403029759574 1108 68-69 8.81035003847656 1108 70-71 8.77064725936337 1108 72-73 8.438740535518944 1108 74-75 8.597584802170589 1108 76-77 8.169099735851901 1108 78-79 7.883913643339948 1108 80-81 7.666794182344576 1108 82-83 7.735304234678473 1108 84-85 7.704095790584134 1108 86-87 7.3175191233767904 1108 88-89 7.302333492174386 1108 90-91 6.994449169266163 1108 92-93 6.574560780705728 1108 94-95 6.206576601855961 1108 96-97 5.855091482438953 1108 98-99 5.49750301020701 1108 100-101 4.8483961851304755 1109 1 6.435014816669774 1109 2 7.899625260511538 1109 3 7.897757049137251 1109 4 8.63786977765685 1109 5 9.729828122338581 1109 6 8.943811875282073 1109 7 7.273586047191841 1109 8 7.966487246084771 1109 9 7.327297918005719 1109 10-11 5.315368090620872 1109 12-13 6.05274369903756 1109 14-15 7.303535564171476 1109 16-17 6.322602126877872 1109 18-19 5.620777441331583 1109 20-21 7.548628591655923 1109 22-23 8.824751864153832 1109 24-25 8.337698095514916 1109 26-27 8.669816521823549 1109 28-29 8.847386340200341 1109 30-31 6.620769280331935 1109 32-33 5.6498285659656595 1109 34-35 7.344576251191867 1109 36-37 5.864788623100196 1109 38-39 6.107109534835757 1109 40-41 6.829890618157972 1109 42-43 5.963040347129887 1109 44-45 6.557695384453417 1109 46-47 5.774832644471729 1109 48-49 6.866449363862859 1109 50-51 8.628082598475698 1109 52-53 8.627086445936094 1109 54-55 8.948732616708366 1109 56-57 7.976604550062593 1109 58-59 7.780070022479801 1109 60-61 8.025084448747531 1109 62-63 8.475202914755638 1109 64-65 8.385338512084271 1109 66-67 7.902281179544932 1109 68-69 7.793466271341249 1109 70-71 7.381345558200005 1109 72-73 6.7520274831595 1109 74-75 5.662416321568005 1109 76-77 5.555918911441964 1109 78-79 6.510694598608609 1109 80-81 6.577177901604751 1109 82-83 6.421407078461886 1109 84-85 6.143900380214376 1109 86-87 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-14.580592485935494 2313 68-69 -14.131578212644516 2313 70-71 -13.79325408592811 2313 72-73 -13.379645114705271 2313 74-75 -12.944567664197121 2313 76-77 -12.521483223789353 2313 78-79 -12.143664831996375 2313 80-81 -11.691950212274255 2313 82-83 -11.244740608370854 2313 84-85 -10.841419904483132 2313 86-87 -10.376875495457291 2313 88-89 -9.963361575090188 2313 90-91 -9.491425270195718 2313 92-93 -8.9597441520297 2313 94-95 -8.402616223256146 2313 96-97 -7.795132732807685 2313 98-99 -7.140613581721242 2313 100-101 -6.26371143818791 2314 1 -18.56987842176439 2314 2 -17.325018867602342 2314 3 -17.94832836011542 2314 4 -19.41906972412251 2314 5 -18.729691450615157 2314 6 -19.709657875607608 2314 7 -19.90479473572631 2314 8 -20.32399318096149 2314 9 -21.60152770477008 2314 10-11 -21.98134009443251 2314 12-13 -21.882532101674183 2314 14-15 -21.8212420158997 2314 16-17 -20.62713096920754 2314 18-19 -19.586295512405073 2314 20-21 -19.257421230408134 2314 22-23 -18.860301516629086 2314 24-25 -18.68632325679825 2314 26-27 -18.420708389208478 2314 28-29 -18.229126115315676 2314 30-31 -18.211081253475896 2314 32-33 -18.043454352183804 2314 34-35 -17.727042965889986 2314 36-37 -17.25576297832329 2314 38-39 -17.141778365520114 2314 40-41 -17.015749951237044 2314 42-43 -16.857021930450184 2314 44-45 -16.450756572841957 2314 46-47 -16.135976964069194 2314 48-49 -15.878879817631802 2314 50-51 -13.773385373054554 2314 52-53 -15.586881525594158 2314 54-55 -16.30104496336281 2314 56-57 -16.26650043214381 2314 58-59 -15.989947771114505 2314 60-61 -15.705565017444638 2314 62-63 -15.292368259621586 2314 64-65 -14.930497786848111 2314 66-67 -14.580592485935494 2314 68-69 -14.131578212644516 2314 70-71 -13.79325408592811 2314 72-73 -13.379645114705271 2314 74-75 -12.944567664197121 2314 76-77 -12.521483223789353 2314 78-79 -12.143664831996375 2314 80-81 -11.691950212274255 2314 82-83 -11.244740608370854 2314 84-85 -10.841419904483132 2314 86-87 -10.376875495457291 2314 88-89 -9.963361575090188 2314 90-91 -9.491425270195718 2314 92-93 -8.9597441520297 2314 94-95 -8.402616223256146 2314 96-97 -7.795132732807685 2314 98-99 -7.140613581721242 2314 100-101 -6.26371143818791 2315 1 -18.56987842176439 2315 2 -17.325018867602342 2315 3 -17.94832836011542 2315 4 -19.41906972412251 2315 5 -18.729691450615157 2315 6 -19.709657875607608 2315 7 -19.90479473572631 2315 8 -20.32399318096149 2315 9 -21.60152770477008 2315 10-11 -21.98134009443251 2315 12-13 -21.882532101674183 2315 14-15 -21.8212420158997 2315 16-17 -20.62713096920754 2315 18-19 -19.586295512405073 2315 20-21 -19.257421230408134 2315 22-23 -18.860301516629086 2315 24-25 -18.68632325679825 2315 26-27 -18.420708389208478 2315 28-29 -18.229126115315676 2315 30-31 -18.211081253475896 2315 32-33 -18.043454352183804 2315 34-35 -17.727042965889986 2315 36-37 -17.25576297832329 2315 38-39 -17.141778365520114 2315 40-41 -17.015749951237044 2315 42-43 -16.857021930450184 2315 44-45 -16.450756572841957 2315 46-47 -16.135976964069194 2315 48-49 -15.878879817631802 2315 50-51 -13.773385373054554 2315 52-53 -15.586881525594158 2315 54-55 -16.30104496336281 2315 56-57 -16.26650043214381 2315 58-59 -15.989947771114505 2315 60-61 -15.705565017444638 2315 62-63 -15.292368259621586 2315 64-65 -14.930497786848111 2315 66-67 -14.580592485935494 2315 68-69 -14.131578212644516 2315 70-71 -13.79325408592811 2315 72-73 -13.379645114705271 2315 74-75 -12.944567664197121 2315 76-77 -12.521483223789353 2315 78-79 -12.143664831996375 2315 80-81 -11.691950212274255 2315 82-83 -11.244740608370854 2315 84-85 -10.841419904483132 2315 86-87 -10.376875495457291 2315 88-89 -9.963361575090188 2315 90-91 -9.491425270195718 2315 92-93 -8.9597441520297 2315 94-95 -8.402616223256146 2315 96-97 -7.795132732807685 2315 98-99 -7.140613581721242 2315 100-101 -6.26371143818791 2316 1 -18.56987842176439 2316 2 -17.325018867602342 2316 3 -17.94832836011542 2316 4 -19.41906972412251 2316 5 -18.729691450615157 2316 6 -19.709657875607608 2316 7 -19.90479473572631 2316 8 -20.32399318096149 2316 9 -21.60152770477008 2316 10-11 -21.98134009443251 2316 12-13 -21.882532101674183 2316 14-15 -21.8212420158997 2316 16-17 -20.62713096920754 2316 18-19 -19.586295512405073 2316 20-21 -19.257421230408134 2316 22-23 -18.860301516629086 2316 24-25 -18.68632325679825 2316 26-27 -18.420708389208478 2316 28-29 -18.229126115315676 2316 30-31 -18.211081253475896 2316 32-33 -18.043454352183804 2316 34-35 -17.727042965889986 2316 36-37 -17.25576297832329 2316 38-39 -17.141778365520114 2316 40-41 -17.015749951237044 2316 42-43 -16.857021930450184 2316 44-45 -16.450756572841957 2316 46-47 -16.135976964069194 2316 48-49 -15.878879817631802 2316 50-51 -13.773385373054554 2316 52-53 -15.586881525594158 2316 54-55 -16.30104496336281 2316 56-57 -16.26650043214381 2316 58-59 -15.989947771114505 2316 60-61 -15.705565017444638 2316 62-63 -15.292368259621586 2316 64-65 -14.930497786848111 2316 66-67 -14.580592485935494 2316 68-69 -14.131578212644516 2316 70-71 -13.79325408592811 2316 72-73 -13.379645114705271 2316 74-75 -12.944567664197121 2316 76-77 -12.521483223789353 2316 78-79 -12.143664831996375 2316 80-81 -11.691950212274255 2316 82-83 -11.244740608370854 2316 84-85 -10.841419904483132 2316 86-87 -10.376875495457291 2316 88-89 -9.963361575090188 2316 90-91 -9.491425270195718 2316 92-93 -8.9597441520297 2316 94-95 -8.402616223256146 2316 96-97 -7.795132732807685 2316 98-99 -7.140613581721242 2316 100-101 -6.26371143818791 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 545969.0 3 23842.0 4 82064.0 5 45034.0 6 26461.0 7 26225.0 8 25187.0 9 25283.0 10 26745.0 11 28006.0 12 29073.0 13 29640.0 14 30545.0 15 31206.0 16 32212.0 17 32705.0 18 32429.0 19 32743.0 20 34207.0 21 36340.0 22 38417.0 23 41016.0 24 43381.0 25 46307.0 26 49277.0 27 52728.0 28 56578.0 29 61168.0 30 65690.0 31 71350.0 32 76701.0 33 81803.0 34 84275.0 35 78739.0 36 57275.0 37 23419.0 38 2627.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73929957767829 21.051877398654376 14.19759485165081 23.011228172016523 2 14.599339691659589 21.040628793624165 42.27594671440325 22.084084800313004 3 18.633651338121272 26.652645378457734 30.331200569722643 24.382502713698358 4 11.841370572002276 17.638906785211912 40.360682494174746 30.159040148611066 5 12.27006057745923 38.427078812496404 36.169785429667364 13.133075180377004 6 28.897504415158405 39.446743160222006 18.463936176203894 13.1918162484157 7 25.502291383611254 33.33786846091292 23.5915464389783 17.568293716497525 8 23.835665154267662 37.46527458784411 21.00197668870561 17.697083569182624 9 25.03944530725917 16.940367517831753 21.834544352233547 36.185642822675526 10-11 23.467971574169024 27.692465628933853 29.568569351353414 19.27099344554371 12-13 24.19480648887155 25.21425373626574 29.74052092294781 20.850418851914903 14-15 21.486435735821914 26.914562437186078 27.476507150305817 24.122494676686188 16-17 20.729195383887962 29.943399800705695 28.239695298410112 21.08770951699623 18-19 20.494316330352962 28.69950218142844 30.49090830528237 20.315273182936224 20-21 21.265667415085847 28.082532685260485 30.534833915455707 20.11696598419796 22-23 20.88225791225287 28.021035962307856 30.194038503217023 20.90266762222225 24-25 20.7057838913367 28.204749948709935 30.503521917955435 20.585944241997936 26-27 20.65907065144629 28.414627218415944 30.28819432793803 20.638107802199738 28-29 20.66380999363909 28.36146665493914 30.400877525083526 20.573845826338243 30-31 20.934784840521008 28.255575732330847 30.328701248511535 20.48093817863661 32-33 20.695083680321495 28.38796448303333 30.249154165954412 20.667797670690764 34-35 20.92681106194051 28.343505008762115 30.282134399322402 20.447549529974975 36-37 20.948977366041834 28.224801894409744 30.147205264894588 20.679015474653834 38-39 20.831250446332927 28.52668530400104 30.199131640860706 20.44293260880532 40-41 21.023378398533445 28.392177370988986 30.140066493353494 20.44437773712407 42-43 20.92305635275747 28.339055677855086 29.91005015607118 20.82783781331627 44-45 20.91726578184531 28.666472246281398 29.78192791593029 20.634334055943004 46-47 20.993337743863375 28.641779236611846 29.478831091750152 20.886051927774627 48-49 20.976783222632996 28.76341928255603 29.44370582712172 20.81609166768925 50-51 21.255225963881585 28.8869733007536 29.017203823366565 20.84059691199825 52-53 20.843439713829913 29.15068666578613 29.116297074937048 20.889576545446907 54-55 20.70860301841444 29.065037512497476 29.289667868194623 20.936691600893457 56-57 20.774732099950803 29.135843484563452 29.352742555191107 20.736681860294638 58-59 20.696216946729717 29.130604931156583 29.34977513583708 20.82340298627662 60-61 20.73689360110204 29.267516800188176 29.276056087792636 20.719533510917152 62-63 20.708000990865692 29.136786263157205 29.29543084874385 20.85978189723325 64-65 20.570011708963996 29.045514389539424 29.376646094879945 21.007827806616636 66-67 20.488258202193823 29.105070447090526 29.11988649857276 21.28678485214289 68-69 20.542498379663364 29.13944131853743 29.179789807154194 21.13827049464501 70-71 20.71720826833002 28.873588271775102 29.256770008043638 21.152433451851245 72-73 20.675307316083803 29.082682413370524 29.119275661396994 21.122734609148676 74-75 20.56275913181015 29.353672565079275 28.83045975400678 21.253108549103793 76-77 20.66310130547367 29.078940935280222 29.065700652387612 21.1922571068585 78-79 20.48618222418953 29.185618197794227 28.94273429377781 21.385465284238432 80-81 20.515655982725274 29.309205582769565 28.680934095988153 21.494204338517008 82-83 20.801128903447623 29.080769386953992 28.880805565023927 21.237296144574458 84-85 20.809244449915344 29.093167091680485 28.67127011171482 21.42631834668936 86-87 20.641791296980024 29.15489362318456 28.77113565563531 21.432179424200108 88-89 20.787777285288893 29.13193380552771 28.65252066718031 21.427768242003086 90-91 20.688101958730414 29.099564471808932 28.76673356270266 21.44560000675799 92-93 20.71271012903078 29.158566796626594 28.611456021778242 21.517267052564385 94-95 20.572013535925183 29.03256281077569 28.63742227348112 21.758001379818 96-97 20.595970896837844 29.054441260744984 28.62403907080407 21.725548771613095 98-99 20.504595335296198 29.07952922801344 28.365415903160745 22.05045953352962 100-101 20.720408332275802 29.14649164669273 28.346908463052234 21.78619155797924 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 126362.0 1 114256.5 2 75575.0 3 38477.5 4 24081.0 5 19129.0 6 17916.5 7 17886.5 8 18384.5 9 18296.0 10 16719.0 11 14262.0 12 11411.5 13 8608.5 14 6583.0 15 5539.0 16 5164.0 17 5513.5 18 6466.0 19 7462.5 20 8580.5 21 9891.5 22 11458.0 23 13299.5 24 15167.5 25 17415.5 26 20014.5 27 22972.5 28 26031.0 29 29181.0 30 32926.5 31 36898.0 32 41081.0 33 45439.0 34 49421.5 35 52644.5 36 56606.0 37 60158.0 38 61763.0 39 62693.5 40 62835.5 41 62705.0 42 62355.0 43 62412.5 44 62159.5 45 61334.5 46 60311.5 47 58207.0 48 55725.0 49 53088.5 50 49764.0 51 45838.0 52 41670.0 53 37292.0 54 32758.5 55 28151.0 56 23638.5 57 19276.5 58 15396.5 59 12092.5 60 9280.5 61 6927.0 62 4982.0 63 3443.0 64 2278.5 65 1484.0 66 963.0 67 608.5 68 393.0 69 265.0 70 164.5 71 93.0 72 63.5 73 55.5 74 36.0 75 18.0 76 15.5 77 9.5 78 7.0 79 6.0 80 3.5 81 1.5 82 2.0 83 2.0 84 1.5 85 1.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 22.153061103397533 2 29.14730558818992 3 22.947488340631566 4 29.269393712841325 5 32.407134465449104 6 24.98363534954371 7 23.941424282340655 8 19.913370595844164 9 11.761282589863173 10-11 15.38305623791045 12-13 15.442225307653054 14-15 19.61294456904838 16-17 22.948366501714787 18-19 26.94580322071511 20-21 28.996190679625478 22-23 28.599048263258453 24-25 27.11808893161024 26-27 28.55834431034687 28-29 25.524440172309443 30-31 27.40878398424057 32-33 26.412711975031744 34-35 24.453890609609928 36-37 27.424994363155207 38-39 27.548055585223157 40-41 24.57161166292855 42-43 25.44661611306917 44-45 24.150046874814578 46-47 27.460998967567374 48-49 22.826942931396633 50-51 28.413495199781647 52-53 22.012697734581746 54-55 23.13351608577498 56-57 25.273974390924085 58-59 24.553906036764094 60-61 24.122420401818033 62-63 23.925664851009294 64-65 22.446936523194132 66-67 22.947796883714858 68-69 23.942207507090558 70-71 22.426976159113295 72-73 22.298693438712665 74-75 19.554677394472332 76-77 21.844114540686153 78-79 20.965834787045935 80-81 21.983362407585414 82-83 22.850273535310382 84-85 19.790712853192826 86-87 21.34363392549871 88-89 20.215268135806426 90-91 21.33110232950028 92-93 21.32464665883442 94-95 20.35992737370501 96-97 19.280263923006633 98-99 18.67214923991598 100-101 20.32767275445904 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2106675.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.16638898382374 #Duplication Level Percentage of deduplicated Percentage of total 1 91.0855895955787 73.0200280634009 2 6.122569072061108 9.81648507622359 3 1.3822535385884414 3.3243082454624338 4 0.49209481232492375 1.5779785656704632 5 0.26572029176314643 1.0650918135189764 6 0.14971651656225174 0.7201339502419537 7 0.10410742303099373 0.5842141319564292 8 0.07132468905600547 0.4574274213611208 9 0.05194734275157484 0.37479877971288883 >10 0.2614829099058641 3.5552114839025775 >50 0.010034425205410827 0.5476467914628677 >100 0.0029212215811177367 0.3597135507641645 >500 5.954039760693212E-5 0.03966478238766153 >1k 1.1908079521386425E-4 0.15269270620713507 >5k 0.0 0.0 >10k+ 5.954039760693212E-5 4.404604637726857 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 92279 4.3803149512857935 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.746816666073315E-5 0.0 4 0.0 0.0 0.0 4.746816666073315E-5 0.0 5 0.0 0.0 0.0 4.746816666073315E-5 0.0 6 0.0 0.0 0.0 4.746816666073315E-5 0.0 7 0.0 0.0 0.0 4.746816666073315E-5 0.0 8 0.0 0.0 0.0 4.746816666073315E-5 0.0 9 0.0 0.0 0.0 4.746816666073315E-5 0.0 10-11 0.0 0.0 0.0 9.49363333214663E-5 0.0 12-13 0.0 4.746816666073315E-5 0.0 9.49363333214663E-5 0.0 14-15 0.0 4.746816666073315E-5 0.0 9.49363333214663E-5 0.0 16-17 0.0 4.746816666073315E-5 0.0 9.49363333214663E-5 0.0 18-19 0.0 4.746816666073315E-5 0.0 9.49363333214663E-5 2.3734083330366574E-5 20-21 0.0 4.746816666073315E-5 0.0 2.3734083330366574E-4 4.746816666073315E-5 22-23 0.0 4.746816666073315E-5 0.0 3.0854308329476544E-4 4.746816666073315E-5 24-25 0.0 4.746816666073315E-5 0.0 3.32277166625132E-4 4.746816666073315E-5 26-27 0.0 4.746816666073315E-5 0.0 4.7468166660733147E-4 4.746816666073315E-5 28-29 0.0 4.746816666073315E-5 0.0 5.696179999287978E-4 4.746816666073315E-5 30-31 0.0 4.746816666073315E-5 0.0 0.0016376517497952935 4.746816666073315E-5 32-33 0.0 4.746816666073315E-5 0.0 0.0037499851661979187 4.746816666073315E-5 34-35 0.0 4.746816666073315E-5 0.0 0.007381299915744004 4.746816666073315E-5 36-37 0.0 4.746816666073315E-5 0.0 0.012555330081763917 4.746816666073315E-5 38-39 4.746816666073315E-5 4.746816666073315E-5 0.0 0.021431877247321016 4.746816666073315E-5 40-41 4.746816666073315E-5 4.746816666073315E-5 0.0 0.03080684016281581 4.746816666073315E-5 42-43 4.746816666073315E-5 4.746816666073315E-5 0.0 0.038710289911827886 4.746816666073315E-5 44-45 4.746816666073315E-5 4.746816666073315E-5 0.0 0.04867860491058184 4.746816666073315E-5 46-47 4.746816666073315E-5 4.746816666073315E-5 0.0 0.06303772532545361 4.746816666073315E-5 48-49 4.746816666073315E-5 4.746816666073315E-5 0.0 0.0772544412403432 4.746816666073315E-5 50-51 7.120224999109972E-5 4.746816666073315E-5 0.0 0.09275279765507258 4.746816666073315E-5 52-53 9.49363333214663E-5 4.746816666073315E-5 0.0 0.11195367106933912 4.746816666073315E-5 54-55 9.49363333214663E-5 4.746816666073315E-5 0.0 0.1326260576500884 4.746816666073315E-5 56-57 9.49363333214663E-5 4.746816666073315E-5 0.0 0.15866234706350055 4.746816666073315E-5 58-59 9.49363333214663E-5 1.4240449998219944E-4 0.0 0.18719071522660116 4.746816666073315E-5 60-61 9.49363333214663E-5 1.4240449998219944E-4 0.0 0.21726179880617563 4.746816666073315E-5 62-63 9.49363333214663E-5 1.4240449998219944E-4 0.0 0.2500623019687422 4.746816666073315E-5 64-65 9.49363333214663E-5 1.6613858331256603E-4 0.0 0.285402352047658 4.746816666073315E-5 66-67 9.49363333214663E-5 1.898726666429326E-4 0.0 0.32192910629309224 4.746816666073315E-5 68-69 9.49363333214663E-5 1.898726666429326E-4 0.0 0.3664542466208599 4.746816666073315E-5 70-71 9.49363333214663E-5 1.898726666429326E-4 0.0 0.4172926531145051 4.746816666073315E-5 72-73 9.49363333214663E-5 1.898726666429326E-4 0.0 0.4691041570246953 4.746816666073315E-5 74-75 9.49363333214663E-5 1.898726666429326E-4 0.0 0.5221498332680646 9.49363333214663E-5 76-77 9.49363333214663E-5 1.898726666429326E-4 0.0 0.5800847306774894 9.49363333214663E-5 78-79 9.49363333214663E-5 1.898726666429326E-4 0.0 0.6499816060854189 9.49363333214663E-5 80-81 9.49363333214663E-5 1.898726666429326E-4 0.0 0.7264290884925297 9.49363333214663E-5 82-83 9.49363333214663E-5 1.898726666429326E-4 0.0 0.8079319306490085 9.49363333214663E-5 84-85 9.49363333214663E-5 2.1360674997329915E-4 0.0 0.8896009113887999 9.49363333214663E-5 86-87 9.49363333214663E-5 2.3734083330366574E-4 0.0 0.9754233567114055 9.49363333214663E-5 88-89 9.49363333214663E-5 2.3734083330366574E-4 0.0 1.0706919671994968 9.49363333214663E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1920 0.0 56.83255 1 GTATCAA 3355 0.0 39.242744 1 ATCAACG 3905 0.0 33.028454 3 TCAACGC 4135 0.0 31.795996 4 CAACGCA 4245 0.0 31.206076 5 TATCAAC 4210 0.0 30.905725 2 AACGCAG 4505 0.0 29.61057 6 GTACATG 3375 0.0 25.045454 1 ACGCAGA 5060 0.0 24.981579 7 CGCAGAG 5105 0.0 24.510479 8 TACATGG 3450 0.0 24.053776 2 ACATGGG 3450 0.0 23.073172 3 GTGGTAT 1080 0.0 22.29425 1 GCAGAGT 5520 0.0 22.102295 9 GAGTACT 3610 0.0 20.68892 12-13 CAGAGTA 5350 0.0 20.679754 10-11 GTGTAGC 760 0.0 20.222109 1 AGAGTAC 4960 0.0 19.627243 10-11 TGGTATC 1100 0.0 18.627502 2 GTACTTT 4225 0.0 18.456427 14-15 >>END_MODULE