FastQCFastQC Report
Sat 18 Jun 2016
SRR1931020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106675
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59770.28371723213120204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55070.2614071938006574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32750.15545824581390105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.056.0840951
GTATCAA58050.040.116951
ATCAACG72600.031.8075683
CAACGCA73600.031.3944425
TCAACGC74150.031.2708364
TATCAAC75500.030.5901832
AACGCAG77350.029.9950876
ACGCAGA87200.026.498067
CGCAGAG87400.026.3288528
GTGGTAT17650.024.7729171
GGCGTCG1551.4255602E-624.488378
GCGTCGG1452.3560793E-522.905079
GTACATG59200.022.6392211
GCAGAGT100400.022.541689
ACGGGCG1503.0614203E-522.1415675
TGGTATC18450.021.8935072
TACATGG59350.021.538942
ACATGGG55950.021.4857863
AGAGTAC93700.020.45706710-11
GAGTACT73600.019.91967612-13
Produced by FastQC (version 0.11.5)