FastQCFastQC Report
Sat 18 Jun 2016
SRR1931019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106923
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN925174.391095450569385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.049.838521
GTATCAA31300.045.1603281
ATCAACG37950.036.892883
TCAACGC38600.036.5273934
CAACGCA39200.035.8334925
TATCAAC39850.034.6923562
AACGCAG42850.033.439526
ACGCAGA48050.028.6001937
CGCAGAG48900.028.1565368
GCAGAGT53150.024.3080379
GTACATG33700.024.0115131
GTATAGG4300.022.6296311
TACATGG34200.022.3078942
AGAGTAC47900.020.81033110-11
GTGGTAT8450.020.6069891
GAGTACT36350.020.27389712-13
ACATGGG36550.020.2553393
CAGAGTA51000.019.72511710-11
GAGTACA26550.018.9040091
AGTACTT39300.018.81314712-13
Produced by FastQC (version 0.11.5)