##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931019_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2106923 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.17641366105928 28.0 2.0 31.0 2.0 33.0 2 19.873219856634535 27.0 2.0 31.0 2.0 33.0 3 20.53618997941548 27.0 2.0 31.0 2.0 33.0 4 22.04401537218019 30.0 2.0 35.0 2.0 37.0 5 21.37521684465925 30.0 2.0 35.0 2.0 37.0 6 22.370546526854564 30.0 2.0 35.0 2.0 37.0 7 22.567237151049184 30.0 2.0 35.0 2.0 37.0 8 23.00202522825941 32.0 2.0 35.0 2.0 37.0 9 24.32787814267536 32.0 2.0 37.0 2.0 39.0 10-11 24.694241792414815 32.0 2.0 37.0 2.0 39.0 12-13 24.582184778466036 32.0 2.0 37.0 2.0 39.0 14-15 24.524407394100308 32.0 2.0 38.0 2.0 40.0 16-17 23.342118814973304 31.5 2.0 38.0 2.0 40.0 18-19 22.266979144468024 29.0 2.0 38.0 2.0 40.0 20-21 21.920624768916568 28.0 2.0 37.5 2.0 40.0 22-23 21.54132329468139 27.0 2.0 37.0 2.0 40.0 24-25 21.361719436353393 27.0 2.0 37.0 2.0 39.5 26-27 21.079165683795754 27.0 2.0 37.0 2.0 39.0 28-29 20.8758376551967 26.5 2.0 37.0 2.0 39.0 30-31 20.85387553318275 26.0 2.0 36.5 2.0 39.0 32-33 20.720522297207825 25.5 2.0 36.5 2.0 39.0 34-35 20.38603380379824 25.0 2.0 36.0 2.0 39.0 36-37 19.91268238089384 24.0 2.0 36.0 2.0 39.0 38-39 19.798818229237614 24.0 2.0 35.5 2.0 39.0 40-41 19.655928574513638 24.0 2.0 35.0 2.0 39.0 42-43 19.50349609359241 24.0 2.0 35.0 2.0 39.0 44-45 19.110800204848495 23.0 2.0 35.0 2.0 38.0 46-47 18.754885441945433 20.0 2.0 35.0 2.0 38.0 48-49 18.507516411373363 18.5 2.0 34.5 2.0 38.0 50-51 16.279428104396793 11.0 2.0 33.0 2.0 37.5 52-53 18.17058288319032 19.5 2.0 34.0 2.0 38.0 54-55 18.92136162546045 22.0 2.0 35.5 2.0 39.0 56-57 18.88032951370316 21.5 2.0 36.0 2.0 39.0 58-59 18.587424647222512 19.5 2.0 35.0 2.0 39.0 60-61 18.29200877298316 18.0 2.0 35.0 2.0 39.0 62-63 17.844886357973216 14.5 2.0 34.0 2.0 38.5 64-65 17.480336728015217 12.0 2.0 34.0 2.0 38.0 66-67 17.10960699560449 8.0 2.0 34.0 2.0 38.0 68-69 16.6646870815877 7.5 2.0 33.0 2.0 37.5 70-71 16.296310543859455 4.5 2.0 32.5 2.0 37.0 72-73 15.890851967537493 2.0 2.0 32.0 2.0 36.0 74-75 15.432812921971994 2.0 2.0 31.5 2.0 36.0 76-77 15.01778968666629 2.0 2.0 31.0 2.0 35.5 78-79 14.641014408215202 2.0 2.0 31.0 2.0 35.0 80-81 14.207066418658869 2.0 2.0 30.5 2.0 34.5 82-83 13.744311016586748 2.0 2.0 30.0 2.0 34.0 84-85 13.338851016387405 2.0 2.0 29.5 2.0 34.0 86-87 12.854525058580688 2.0 2.0 29.0 2.0 34.0 88-89 12.428861662243946 2.0 2.0 29.0 2.0 34.0 90-91 11.949586909440924 2.0 2.0 28.5 2.0 34.0 92-93 11.405472577782861 2.0 2.0 26.5 2.0 34.0 94-95 10.838227595408089 2.0 2.0 25.5 2.0 33.5 96-97 10.194349295156966 2.0 2.0 24.5 2.0 33.0 98-99 9.529156974412448 2.0 2.0 21.0 2.0 32.0 100-101 8.622366360801985 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.698052373175312 1101 2 9.413830728011636 1101 3 8.68449761436846 1101 4 10.811588605883973 1101 5 11.628035240333372 1101 6 10.791971313559849 1101 7 10.547549986154802 1101 8 9.681571395205381 1101 9 9.557881560692373 1101 10-11 9.087276049770654 1101 12-13 8.981412892756385 1101 14-15 9.653692395039013 1101 16-17 10.551354471606171 1101 18-19 11.457097417194234 1101 20-21 11.597217502203723 1101 22-23 11.88737130911965 1101 24-25 11.668721444029618 1101 26-27 11.868241923279644 1101 28-29 11.818483973097393 1101 30-31 11.35962920712787 1101 32-33 11.3555791555594 1101 34-35 11.244541385397866 1101 36-37 11.561180412592961 1101 38-39 11.163721236327415 1101 40-41 10.833042906238507 1101 42-43 10.721017476070887 1101 44-45 10.463934934940166 1101 46-47 10.30780537608388 1101 48-49 10.416830595527802 1101 50-51 12.288371104374727 1101 52-53 11.353658391661217 1101 54-55 10.95691447205678 1101 56-57 11.006260911363611 1101 58-59 10.680567388119492 1101 60-61 10.112347225601994 1101 62-63 10.327097551835827 1101 64-65 9.992337648018562 1101 66-67 9.576042581857283 1101 68-69 9.74005957982525 1101 70-71 9.43255198054816 1101 72-73 9.211191401788215 1101 74-75 8.934403663437845 1101 76-77 9.003192369388684 1101 78-79 8.59650510613315 1101 80-81 8.362105089683753 1101 82-83 7.909471057452189 1101 84-85 7.554572733895453 1101 86-87 7.230918702338743 1101 88-89 7.175179176926868 1101 90-91 6.966346878858211 1101 92-93 6.664231374059517 1101 94-95 6.249784008311648 1101 96-97 6.055945684564994 1101 98-99 5.824262292439487 1101 100-101 5.318824223803132 1102 1 7.7847929130648446 1102 2 9.068488186870901 1102 3 8.485986676403233 1102 4 10.232024067038221 1102 5 11.027306934772888 1102 6 10.07903602804199 1102 7 9.887476653780496 1102 8 9.259694879296074 1102 9 8.92062004981797 1102 10-11 8.235476571327101 1102 12-13 8.610136384207077 1102 14-15 9.049934361765121 1102 16-17 10.013071593310475 1102 18-19 11.011088252320178 1102 20-21 11.096934811253508 1102 22-23 11.181180042764552 1102 24-25 11.17779118171249 1102 26-27 11.205956408765392 1102 28-29 10.889414310687258 1102 30-31 10.793463424776487 1102 32-33 10.94056793155777 1102 34-35 10.580403743341485 1102 36-37 10.94740545968024 1102 38-39 10.669240400766569 1102 40-41 10.203079923698436 1102 42-43 9.747996270904167 1102 44-45 9.56834303194498 1102 46-47 9.780477399381631 1102 48-49 9.619579388654902 1102 50-51 11.459286220144195 1102 52-53 10.542393073658989 1102 54-55 10.264671453185066 1102 56-57 10.074717912230085 1102 58-59 10.036177339844574 1102 60-61 9.937329327417569 1102 62-63 9.940359186492064 1102 64-65 9.635274096082046 1102 66-67 9.578865309540593 1102 68-69 9.213286530476445 1102 70-71 8.823359882847493 1102 72-73 8.830656346437658 1102 74-75 8.334506076180013 1102 76-77 8.207771753691139 1102 78-79 8.02291638633843 1102 80-81 7.67951559039801 1102 82-83 7.4165630039382915 1102 84-85 6.919530723343891 1102 86-87 6.881131515096973 1102 88-89 6.746357914552867 1102 90-91 6.42148307233257 1102 92-93 6.041102947059015 1102 94-95 5.757881891307703 1102 96-97 5.614480483988238 1102 98-99 5.413870096382441 1102 100-101 4.889009988557424 1103 1 7.498159239087354 1103 2 8.89817394016275 1103 3 8.401678071923087 1103 4 10.468776290458575 1103 5 10.868571044464925 1103 6 10.131849475077267 1103 7 9.694476841744844 1103 8 9.058671545336193 1103 9 8.90596789927502 1103 10-11 8.426293977155307 1103 12-13 8.330745932795853 1103 14-15 8.876568992052437 1103 16-17 9.731660112975828 1103 18-19 10.691180148748522 1103 20-21 11.04013660252075 1103 22-23 11.281011369496056 1103 24-25 11.184110864547389 1103 26-27 11.04524112198786 1103 28-29 10.791464276227131 1103 30-31 10.448010719244067 1103 32-33 10.299617755299973 1103 34-35 10.289591160282676 1103 36-37 10.471241688324028 1103 38-39 10.315878971709918 1103 40-41 10.068501161690978 1103 42-43 9.840653418990392 1103 44-45 9.89001536227418 1103 46-47 9.53445164991502 1103 48-49 9.650569598020802 1103 50-51 11.35664861870116 1103 52-53 10.332014803656449 1103 54-55 9.762321692757169 1103 56-57 9.546883731408382 1103 58-59 9.659157341427434 1103 60-61 9.699385014328328 1103 62-63 9.304772177136716 1103 64-65 9.174380366836445 1103 66-67 8.867623063069868 1103 68-69 8.877099313041267 1103 70-71 8.573294548749113 1103 72-73 8.44666950723951 1103 74-75 8.39045576603884 1103 76-77 8.033613100241334 1103 78-79 7.722984234872143 1103 80-81 7.322764620073292 1103 82-83 7.0867791464482455 1103 84-85 6.684125371636219 1103 86-87 6.710927120358653 1103 88-89 6.112081296987768 1103 90-91 5.908852775118071 1103 92-93 5.429390088978691 1103 94-95 5.229973101831069 1103 96-97 5.062378704592428 1103 98-99 4.886951829545618 1103 100-101 4.534651235519492 1104 1 7.746354959584185 1104 2 9.431516712099974 1104 3 8.70210923538163 1104 4 10.701362969977811 1104 5 11.399131637289575 1104 6 10.554812594234619 1104 7 10.210480095050865 1104 8 9.593397976439405 1104 9 9.520709191158822 1104 10-11 8.836534761965186 1104 12-13 8.583531425460865 1104 14-15 9.437388443609304 1104 16-17 10.50596060197584 1104 18-19 11.148546376620516 1104 20-21 11.536568709839688 1104 22-23 11.670185391245006 1104 24-25 11.385201625760754 1104 26-27 11.301800349227268 1104 28-29 11.098646451323273 1104 30-31 10.815235368057717 1104 32-33 11.166757657499975 1104 34-35 10.913945971460393 1104 36-37 11.027846502012014 1104 38-39 10.756088528798411 1104 40-41 10.543325995260275 1104 42-43 10.15315454861054 1104 44-45 10.079697462062317 1104 46-47 10.204827185857024 1104 48-49 10.018390284629028 1104 50-51 11.452518526624567 1104 52-53 10.677551247712639 1104 54-55 10.291247700127315 1104 56-57 10.045381185997652 1104 58-59 10.020351123998147 1104 60-61 9.813007630829816 1104 62-63 9.841018625108926 1104 64-65 9.616632879613704 1104 66-67 9.709386475426406 1104 68-69 9.384788562069794 1104 70-71 9.158783096911092 1104 72-73 8.981865559091583 1104 74-75 8.569814443027724 1104 76-77 8.142062458115037 1104 78-79 7.8520197902938165 1104 80-81 7.763870041245889 1104 82-83 7.61624064397312 1104 84-85 7.072049380833837 1104 86-87 6.85290490951631 1104 88-89 6.47780490655088 1104 90-91 6.315619852464415 1104 92-93 5.99556675152852 1104 94-95 5.756737668910423 1104 96-97 4.912371876666208 1104 98-99 4.649033142114021 1104 100-101 4.076428102907107 1105 1 7.89096145202296 1105 2 8.990799205435632 1105 3 8.439173935548553 1105 4 10.562053071873724 1105 5 10.561954826706355 1105 6 10.503857662621975 1105 7 9.923205374704072 1105 8 9.408491526353451 1105 9 9.404054565981312 1105 10-11 8.819425217631743 1105 12-13 8.509046759674465 1105 14-15 9.328624398665493 1105 16-17 10.415152659575678 1105 18-19 10.800479907600362 1105 20-21 11.260346581734854 1105 22-23 11.533066379809739 1105 24-25 11.40492757807645 1105 26-27 11.764348992691229 1105 28-29 11.194682227002787 1105 30-31 11.003297352661217 1105 32-33 10.896511263718363 1105 34-35 11.037920659419733 1105 36-37 11.297573699999006 1105 38-39 10.91496044887614 1105 40-41 10.742089791069837 1105 42-43 10.510076743981625 1105 44-45 10.637082548885605 1105 46-47 10.505338409355367 1105 48-49 10.423541882825294 1105 50-51 12.14313064949758 1105 52-53 10.964287838061178 1105 54-55 10.43531755737338 1105 56-57 10.186038900439964 1105 58-59 10.153421205465047 1105 60-61 9.962329736512569 1105 62-63 9.758378798506367 1105 64-65 9.597245749889495 1105 66-67 9.5289499922056 1105 68-69 9.22842642051171 1105 70-71 8.97782791616419 1105 72-73 8.644561690036552 1105 74-75 8.283164551401129 1105 76-77 8.11558147951567 1105 78-79 7.860106190054648 1105 80-81 7.695549455282114 1105 82-83 7.482246274407361 1105 84-85 7.037963828129483 1105 86-87 6.923521018537459 1105 88-89 6.699494036823182 1105 90-91 6.401741579463092 1105 92-93 6.154611570781617 1105 94-95 5.718908126570058 1105 96-97 5.514502472844489 1105 98-99 5.088018667413605 1105 100-101 4.3433256339866055 1106 1 7.681998703964954 1106 2 8.876877824745822 1106 3 8.38446612387093 1106 4 10.460300150089619 1106 5 11.129067496410691 1106 6 10.073292098787768 1106 7 9.20443548273401 1106 8 8.802344950968894 1106 9 8.193743401301823 1106 10-11 8.170002437875436 1106 12-13 8.005531797146457 1106 14-15 8.588883674481313 1106 16-17 9.68923786436076 1106 18-19 10.80739485446756 1106 20-21 10.757823801681187 1106 22-23 10.842612146580453 1106 24-25 10.46709380680177 1106 26-27 10.855317951553818 1106 28-29 10.754893330922254 1106 30-31 10.435410562564808 1106 32-33 10.673345834884412 1106 34-35 10.299071214437294 1106 36-37 10.437901903256659 1106 38-39 10.61053755513284 1106 40-41 10.385563341626042 1106 42-43 9.83725996668149 1106 44-45 9.443470974943228 1106 46-47 9.319829923271197 1106 48-49 9.480592672457835 1106 50-51 11.303806919486194 1106 52-53 10.565408379806687 1106 54-55 9.913308947885898 1106 56-57 9.765708972509486 1106 58-59 9.583529136283861 1106 60-61 9.381800004243246 1106 62-63 9.39763859668 1106 64-65 9.269924256005638 1106 66-67 8.882183208611398 1106 68-69 8.663269272089735 1106 70-71 8.54200993060188 1106 72-73 8.202139099597781 1106 74-75 7.923074075460811 1106 76-77 7.638090244124593 1106 78-79 7.4719991969027895 1106 80-81 7.111869518876945 1106 82-83 6.7980185065800445 1106 84-85 6.677974651938294 1106 86-87 6.457373273547189 1106 88-89 6.236930340393354 1106 90-91 6.050166923431249 1106 92-93 5.886518216421811 1106 94-95 5.638041983979262 1106 96-97 5.012783945397908 1106 98-99 4.625772053075231 1106 100-101 4.240505694786279 1107 1 7.363397089426325 1107 2 8.332877200654465 1107 3 8.210756131024954 1107 4 9.863738885518554 1107 5 10.968381043560157 1107 6 9.532781939835033 1107 7 9.493373503157517 1107 8 8.369788899863277 1107 9 5.7143368280159486 1107 10-11 7.4958048240323585 1107 12-13 7.442005391174231 1107 14-15 7.790524931525166 1107 16-17 8.9768110819487 1107 18-19 10.244194290085709 1107 20-21 10.738836556647517 1107 22-23 9.981849230628725 1107 24-25 10.060238846109204 1107 26-27 10.173799670679362 1107 28-29 10.549159154139247 1107 30-31 10.431630482512784 1107 32-33 10.744814914968988 1107 34-35 10.818056375196399 1107 36-37 11.196657047444788 1107 38-39 10.44437918679031 1107 40-41 10.145653314796466 1107 42-43 10.018173907183193 1107 44-45 9.147509643992983 1107 46-47 9.53535006689161 1107 48-49 9.963552437621626 1107 50-51 11.178538643574294 1107 52-53 10.161979271741217 1107 54-55 9.825323976895028 1107 56-57 9.641391324301683 1107 58-59 9.712009974059015 1107 60-61 9.416137832198885 1107 62-63 9.587458443896718 1107 64-65 9.589458732288975 1107 66-67 9.57144427174229 1107 68-69 9.408062555723376 1107 70-71 9.066976463107432 1107 72-73 8.742496908004911 1107 74-75 8.751672367070277 1107 76-77 8.631297295562897 1107 78-79 8.348583444074727 1107 80-81 8.139510969659087 1107 82-83 7.816977068025649 1107 84-85 7.647104163598717 1107 86-87 7.395340696931241 1107 88-89 7.179122886074698 1107 90-91 6.796873868869309 1107 92-93 6.2736002598207765 1107 94-95 6.093249633306618 1107 96-97 5.723661417049788 1107 98-99 5.142115346197285 1107 100-101 4.70141413280313 1108 1 6.788113413979573 1108 2 8.123913466489874 1108 3 7.8346771473292485 1108 4 9.06283616177701 1108 5 10.988429701792835 1108 6 9.85705709220736 1108 7 8.62849273238336 1108 8 6.074223642093688 1108 9 7.224073993145687 1108 10-11 6.0853541638114095 1108 12-13 4.8387620542853504 1108 14-15 7.168082898443561 1108 16-17 8.545898263121781 1108 18-19 8.202904760198969 1108 20-21 8.426513003839698 1108 22-23 9.18246983595543 1108 24-25 9.090461229684607 1108 26-27 7.471504763316847 1108 28-29 9.076700880623534 1108 30-31 9.442594943555054 1108 32-33 9.967640076446994 1108 34-35 9.490610593412647 1108 36-37 9.3557141641055 1108 38-39 8.911631849314876 1108 40-41 9.417581744149398 1108 42-43 8.73051906134512 1108 44-45 8.177763660938222 1108 46-47 9.048047760671128 1108 48-49 9.571375517801279 1108 50-51 11.127666469193025 1108 52-53 10.31935548376245 1108 54-55 9.462997526825298 1108 56-57 9.80468179392616 1108 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2313 58-59 -16.160547752406348 2313 60-61 -15.862947425349958 2313 62-63 -15.437408341183211 2313 64-65 -15.05729970465596 2313 66-67 -14.703422513337639 2313 68-69 -14.285099078379645 2313 70-71 -13.943014655897896 2313 72-73 -13.538808598211785 2313 74-75 -13.076702410904858 2313 76-77 -12.681757766604063 2313 78-79 -12.323485827683712 2313 80-81 -11.904894003697931 2313 82-83 -11.45566946838643 2313 84-85 -11.056714664109984 2313 86-87 -10.589117562393804 2313 88-89 -10.17522880131429 2313 90-91 -9.710897001468169 2313 92-93 -9.166231001280467 2313 94-95 -8.620116263673845 2313 96-97 -7.994144977266375 2313 98-99 -7.353888206200585 2313 100-101 -6.461774144283904 2314 1 -18.750723692101207 2314 2 -17.46241587216969 2314 3 -18.095194135404885 2314 4 -19.570097704179492 2314 5 -18.86199195821604 2314 6 -19.875299765315944 2314 7 -20.08300305101655 2314 8 -20.53873685502127 2314 9 -21.847830134683875 2314 10-11 -22.249532653765154 2314 12-13 -22.16523261948296 2314 14-15 -22.071375601334513 2314 16-17 -20.86976973510281 2314 18-19 -19.781116544727794 2314 20-21 -19.426127919373794 2314 22-23 -19.019926968305555 2314 24-25 -18.848730958508913 2314 26-27 -18.596626617064867 2314 28-29 -18.399331076766615 2314 30-31 -18.365438789245655 2314 32-33 -18.230096275084296 2314 34-35 -17.917289983595786 2314 36-37 -17.447659115965514 2314 38-39 -17.335372145358892 2314 40-41 -17.19959535305433 2314 42-43 -17.068193765996202 2314 44-45 -16.690190178387123 2314 46-47 -16.356745847850842 2314 48-49 -16.111047917618162 2314 50-51 -13.936470237420378 2314 52-53 -15.769415044422193 2314 54-55 -16.496168030209766 2314 56-57 -16.465180611755155 2314 58-59 -16.160547752406348 2314 60-61 -15.862947425349958 2314 62-63 -15.437408341183211 2314 64-65 -15.05729970465596 2314 66-67 -14.703422513337639 2314 68-69 -14.285099078379645 2314 70-71 -13.943014655897896 2314 72-73 -13.538808598211785 2314 74-75 -13.076702410904858 2314 76-77 -12.681757766604063 2314 78-79 -12.323485827683712 2314 80-81 -11.904894003697931 2314 82-83 -11.45566946838643 2314 84-85 -11.056714664109984 2314 86-87 -10.589117562393804 2314 88-89 -10.17522880131429 2314 90-91 -9.710897001468169 2314 92-93 -9.166231001280467 2314 94-95 -8.620116263673845 2314 96-97 -7.994144977266375 2314 98-99 -7.353888206200585 2314 100-101 -6.461774144283904 2315 1 -18.750723692101207 2315 2 -17.46241587216969 2315 3 -18.095194135404885 2315 4 -19.570097704179492 2315 5 -18.86199195821604 2315 6 -19.875299765315944 2315 7 -20.08300305101655 2315 8 -20.53873685502127 2315 9 -21.847830134683875 2315 10-11 -22.249532653765154 2315 12-13 -22.16523261948296 2315 14-15 -22.071375601334513 2315 16-17 -20.86976973510281 2315 18-19 -19.781116544727794 2315 20-21 -19.426127919373794 2315 22-23 -19.019926968305555 2315 24-25 -18.848730958508913 2315 26-27 -18.596626617064867 2315 28-29 -18.399331076766615 2315 30-31 -18.365438789245655 2315 32-33 -18.230096275084296 2315 34-35 -17.917289983595786 2315 36-37 -17.447659115965514 2315 38-39 -17.335372145358892 2315 40-41 -17.19959535305433 2315 42-43 -17.068193765996202 2315 44-45 -16.690190178387123 2315 46-47 -16.356745847850842 2315 48-49 -16.111047917618162 2315 50-51 -13.936470237420378 2315 52-53 -15.769415044422193 2315 54-55 -16.496168030209766 2315 56-57 -16.465180611755155 2315 58-59 -16.160547752406348 2315 60-61 -15.862947425349958 2315 62-63 -15.437408341183211 2315 64-65 -15.05729970465596 2315 66-67 -14.703422513337639 2315 68-69 -14.285099078379645 2315 70-71 -13.943014655897896 2315 72-73 -13.538808598211785 2315 74-75 -13.076702410904858 2315 76-77 -12.681757766604063 2315 78-79 -12.323485827683712 2315 80-81 -11.904894003697931 2315 82-83 -11.45566946838643 2315 84-85 -11.056714664109984 2315 86-87 -10.589117562393804 2315 88-89 -10.17522880131429 2315 90-91 -9.710897001468169 2315 92-93 -9.166231001280467 2315 94-95 -8.620116263673845 2315 96-97 -7.994144977266375 2315 98-99 -7.353888206200585 2315 100-101 -6.461774144283904 2316 1 -18.750723692101207 2316 2 -17.46241587216969 2316 3 -18.095194135404885 2316 4 -19.570097704179492 2316 5 -18.86199195821604 2316 6 -19.875299765315944 2316 7 -20.08300305101655 2316 8 -20.53873685502127 2316 9 -21.847830134683875 2316 10-11 -22.249532653765154 2316 12-13 -22.16523261948296 2316 14-15 -22.071375601334513 2316 16-17 -20.86976973510281 2316 18-19 -19.781116544727794 2316 20-21 -19.426127919373794 2316 22-23 -19.019926968305555 2316 24-25 -18.848730958508913 2316 26-27 -18.596626617064867 2316 28-29 -18.399331076766615 2316 30-31 -18.365438789245655 2316 32-33 -18.230096275084296 2316 34-35 -17.917289983595786 2316 36-37 -17.447659115965514 2316 38-39 -17.335372145358892 2316 40-41 -17.19959535305433 2316 42-43 -17.068193765996202 2316 44-45 -16.690190178387123 2316 46-47 -16.356745847850842 2316 48-49 -16.111047917618162 2316 50-51 -13.936470237420378 2316 52-53 -15.769415044422193 2316 54-55 -16.496168030209766 2316 56-57 -16.465180611755155 2316 58-59 -16.160547752406348 2316 60-61 -15.862947425349958 2316 62-63 -15.437408341183211 2316 64-65 -15.05729970465596 2316 66-67 -14.703422513337639 2316 68-69 -14.285099078379645 2316 70-71 -13.943014655897896 2316 72-73 -13.538808598211785 2316 74-75 -13.076702410904858 2316 76-77 -12.681757766604063 2316 78-79 -12.323485827683712 2316 80-81 -11.904894003697931 2316 82-83 -11.45566946838643 2316 84-85 -11.056714664109984 2316 86-87 -10.589117562393804 2316 88-89 -10.17522880131429 2316 90-91 -9.710897001468169 2316 92-93 -9.166231001280467 2316 94-95 -8.620116263673845 2316 96-97 -7.994144977266375 2316 98-99 -7.353888206200585 2316 100-101 -6.461774144283904 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 530838.0 3 23180.0 4 82576.0 5 45247.0 6 25817.0 7 26052.0 8 25393.0 9 24915.0 10 26047.0 11 27581.0 12 28946.0 13 29195.0 14 29866.0 15 30819.0 16 31962.0 17 32606.0 18 31859.0 19 32807.0 20 34486.0 21 36326.0 22 38401.0 23 40717.0 24 43486.0 25 46417.0 26 49692.0 27 53571.0 28 57606.0 29 61725.0 30 66798.0 31 72114.0 32 78866.0 33 83597.0 34 86710.0 35 81913.0 36 60440.0 37 25351.0 38 2984.0 39 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22296407371328 20.378782074435676 14.177086031666114 23.221167820184927 2 14.940484891445749 20.880114751723912 41.5511773865949 22.628222970235438 3 18.61580117145769 25.934894458292913 30.458452546139963 24.990851824109434 4 12.283789196095986 17.343066845236223 39.48203684300967 30.891107115658112 5 12.625272917559496 38.05681998522068 35.919481367499614 13.398425729720206 6 29.74730216564801 38.57680761367428 18.094524974877828 13.581365245799882 7 26.100416312379217 33.218779526114226 23.0827269238353 17.598077237671266 8 24.503160897112977 36.88304562429196 20.741184845668283 17.87260863292678 9 25.381416416222745 16.8590677896873 21.379043110749958 36.380472683339995 10-11 23.913218229672726 27.255440666007708 29.194219291491528 19.63712181282804 12-13 24.580506661477386 24.672253742366795 29.490461612314746 21.256777983841076 14-15 21.756149290049372 26.4393535700307 27.03936913188435 24.765128008035575 16-17 21.296486251752082 29.60793262173062 27.76730861601094 21.32827251050636 18-19 21.137486598610916 28.408743661855112 29.908177825427163 20.545591914106804 20-21 21.703689374822915 27.602407444339423 30.091922236498082 20.601980944339584 22-23 21.445019301722176 27.532765596033144 29.779662806542888 21.24255229570179 24-25 21.332946087983657 27.715809507738687 29.96159456385411 20.989649840423546 26-27 21.209716158794393 27.943054432562402 29.73653147078946 21.110697937853743 28-29 21.180336428627463 28.096773650542488 29.798100264153927 20.924789656676122 30-31 21.444297981601675 27.71038642208059 29.801187814016572 21.04412778230116 32-33 21.263167299014103 27.89391346041985 29.717587185853585 21.125332054712462 34-35 21.452524812856495 27.91662817588123 29.67835273007965 20.952494281182624 36-37 21.457195516902825 27.796544577864267 29.64123235006883 21.105027555164078 38-39 21.40032182455994 27.916649266041638 29.67539569021319 21.007633219185234 40-41 21.57165666371549 27.86402886068597 29.594842058748167 20.969472416850373 42-43 21.403885277547467 27.901952661858548 29.411981138838122 21.282180921755867 44-45 21.43428892285616 28.197991078391617 29.295099354275195 21.072620644477023 46-47 21.54829681093446 28.228931710463236 28.931645282494962 21.291126196107342 48-49 21.514832710130825 28.271629234665628 28.90675627540094 21.3067817798026 50-51 21.76081863809231 28.438091650724107 28.57078662080496 21.230303090378623 52-53 21.340730497800813 28.59037236008055 28.73663089031126 21.332266251807376 54-55 21.193390620531975 28.608457890461548 28.85953698884226 21.338614500164216 56-57 21.201452120439534 28.686752581577334 28.873631911963855 21.238163386019274 58-59 21.250138318071063 28.598127149807638 28.833448115128284 21.31828641699301 60-61 21.098964160107965 28.702818410403697 28.967222547781695 21.230994881706643 62-63 21.14663404367108 28.618564329117113 28.94049360416223 21.294308023049577 64-65 21.02439298259318 28.531496312858977 28.93172991722545 21.512380787322385 66-67 20.95735077376566 28.659665929746993 28.725773765659547 21.657209530827807 68-69 21.14740849027179 28.60243221567839 28.742275132620655 21.507884161429168 70-71 21.1645305206852 28.506948956390655 28.725840625146798 21.602679897777346 72-73 21.072186610228847 28.65749044566591 28.638244743212997 21.632078200892245 74-75 21.12464619093295 28.823095760886012 28.446307873212263 21.605950174968775 76-77 21.151793374573977 28.762532491082926 28.36779572864489 21.717878405698208 78-79 21.087997269452902 28.796972446025286 28.39316884182288 21.72186144269893 80-81 21.11228961033801 28.90483182324008 28.121037161548383 21.861841404873527 82-83 21.257174320859573 28.7314362866963 28.315470535197207 21.69591885724692 84-85 21.286954550713414 28.815665825319282 28.085989795219668 21.811389828747636 86-87 21.269133257457675 28.833623808952186 28.171351432175673 21.72589150141447 88-89 21.238993167912408 28.822144777858544 28.100678463635898 21.83818359059315 90-91 21.212347266184647 28.889096773553142 28.095532160843774 21.80302379941844 92-93 21.175499402296087 28.632107601741236 28.154154981993702 22.03823801396898 94-95 21.12092680260414 28.633096347843455 28.20124357192092 22.04473327763149 96-97 21.19728831767097 28.511922981748157 28.12745915529958 22.163329545281297 98-99 21.01356001751933 28.639996740590274 27.81167014191528 22.534773099975116 100-101 21.28071047632236 28.669559628180252 27.836141851592437 22.213588043904945 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 124869.0 1 113139.0 2 75019.5 3 38003.0 4 23577.5 5 18570.5 6 17273.5 7 17486.5 8 18131.5 9 18165.5 10 16833.5 11 14258.5 12 11410.0 13 8858.5 14 6766.5 15 5455.5 16 4911.0 17 5133.5 18 5923.5 19 7019.5 20 8081.0 21 9129.0 22 10586.5 23 12256.5 24 14178.0 25 16245.5 26 18290.0 27 20641.0 28 23636.5 29 26885.0 30 30216.0 31 33854.5 32 37875.5 33 41367.5 34 44039.0 35 46870.5 36 49973.5 37 53393.5 38 55994.5 39 57311.5 40 58497.0 41 59802.0 42 60830.5 43 61537.5 44 62139.5 45 62655.0 46 62676.0 47 61685.0 48 60159.0 49 58633.5 50 56016.5 51 52322.0 52 48317.5 53 44152.0 54 39553.5 55 34348.0 56 28837.0 57 23451.5 58 18773.5 59 14767.0 60 11296.0 61 8419.5 62 6280.0 63 4439.5 64 2881.0 65 1893.5 66 1217.0 67 749.0 68 453.5 69 295.0 70 207.5 71 145.5 72 90.0 73 61.0 74 45.0 75 31.0 76 20.0 77 14.0 78 10.0 79 9.5 80 6.0 81 2.0 82 1.5 83 2.0 84 0.5 85 0.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.946174587301005 2 28.89199083212818 3 22.695798565016375 4 29.01548846350816 5 32.110618185856815 6 24.759803751727045 7 23.69502824735408 8 19.72691930364802 9 11.686568517216815 10-11 15.272437578402249 12-13 15.366484679316711 14-15 19.481822544060698 16-17 22.72797819379256 18-19 26.688540587387386 20-21 28.722668080418696 22-23 28.30224455283843 24-25 26.86977644650516 26-27 28.268000301862006 28-29 25.28101406648463 30-31 27.1047636766982 32-33 26.155678209407746 34-35 24.216380949849615 36-37 27.148690293855065 38-39 27.26302764742708 40-41 24.33138277953205 42-43 25.181841007004053 44-45 23.9246047435051 46-47 27.183124395148756 48-49 22.58421404104469 50-51 28.17748916310658 52-53 21.776495866246652 54-55 22.90233197890953 56-57 25.014037057832677 58-59 24.294551817982907 60-61 23.825906309817682 62-63 23.66723890716462 64-65 22.22459007756809 66-67 22.711935841983784 68-69 23.68608155115303 70-71 22.200906250489457 72-73 22.070004456736196 74-75 19.394159160064227 76-77 21.610471763799627 78-79 20.737729855338806 80-81 21.75705994001679 82-83 22.593113274666422 84-85 19.59326468029444 86-87 21.12053928881122 88-89 19.98086783427776 90-91 21.10549365116808 92-93 21.08812234713846 94-95 20.15984922087803 96-97 19.05912081267327 98-99 18.454423820899006 100-101 20.139962400144665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2106923.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.49758120471598 #Duplication Level Percentage of deduplicated Percentage of total 1 90.163900371815 71.67811991542284 2 6.612320798345885 10.513270192362686 3 1.6200599356580938 3.8637253887445886 4 0.6167721551844823 1.9612757796634426 5 0.3039449895773569 1.208144574534624 6 0.1868367693811642 0.8911842747543536 7 0.11274752138525711 0.627420866586845 8 0.07692139567265341 0.48920519190934825 9 0.05944154485653551 0.42529131352495797 >10 0.23695040541087203 2.998362630518474 >50 0.007168703780994611 0.39705738061566437 >100 0.00269605152341259 0.35992448369048435 >500 5.983935208730922E-5 0.03743824912665385 >1k 1.1967870417461844E-4 0.14846858389362982 >5k 0.0 0.0 >10k+ 5.983935208730922E-5 4.401111174651377 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 92517 4.391095450569385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.746257931590286E-5 0.0 10-11 0.0 0.0 0.0 4.746257931590286E-5 0.0 12-13 0.0 0.0 0.0 9.492515863180572E-5 0.0 14-15 0.0 0.0 0.0 9.492515863180572E-5 0.0 16-17 0.0 0.0 0.0 9.492515863180572E-5 0.0 18-19 0.0 0.0 0.0 9.492515863180572E-5 0.0 20-21 0.0 0.0 0.0 9.492515863180572E-5 0.0 22-23 0.0 0.0 0.0 9.492515863180572E-5 0.0 24-25 0.0 0.0 0.0 1.6611902760566E-4 0.0 26-27 0.0 0.0 0.0 2.8477547589541717E-4 0.0 28-29 0.0 0.0 0.0 4.746257931590286E-4 0.0 30-31 0.0 0.0 0.0 0.0016849215657145516 0.0 32-33 0.0 0.0 0.0 0.004414019876378966 0.0 34-35 0.0 0.0 0.0 0.007356699793964944 0.0 36-37 0.0 0.0 0.0 0.013336984787768704 0.0 38-39 0.0 0.0 0.0 0.02254472517505386 0.0 40-41 0.0 0.0 0.0 0.031633809114049255 0.0 42-43 0.0 0.0 0.0 0.04081781821167646 0.0 44-45 0.0 0.0 0.0 0.052541075302704465 0.0 46-47 0.0 2.373128965795143E-5 0.0 0.06732566875960821 0.0 48-49 0.0 4.746257931590286E-5 0.0 0.08317817025111976 0.0 50-51 0.0 4.746257931590286E-5 0.0 0.10031216138416069 0.0 52-53 0.0 4.746257931590286E-5 0.0 0.12036510114512966 0.0 54-55 0.0 4.746257931590286E-5 0.0 0.1431946017960789 0.0 56-57 0.0 4.746257931590286E-5 0.0 0.17060424135101282 0.0 58-59 0.0 4.746257931590286E-5 0.0 0.20088536695455886 0.0 60-61 0.0 4.746257931590286E-5 0.0 0.2334446963652682 0.0 62-63 0.0 4.746257931590286E-5 0.0 0.2680686479762193 0.0 64-65 0.0 4.746257931590286E-5 0.0 0.3029061811940921 0.0 66-67 0.0 4.746257931590286E-5 0.0 0.3425848975021868 0.0 68-69 0.0 4.746257931590286E-5 0.0 0.38606062015555387 0.0 70-71 0.0 4.746257931590286E-5 0.0 0.43829318869270495 0.0 72-73 0.0 9.492515863180572E-5 0.0 0.4924717229818081 2.373128965795143E-5 74-75 0.0 9.492515863180572E-5 0.0 0.5480266720710724 4.746257931590286E-5 76-77 0.0 9.492515863180572E-5 0.0 0.608280416512611 4.746257931590286E-5 78-79 4.746257931590286E-5 9.492515863180572E-5 0.0 0.6777893639207508 7.119386897385429E-5 80-81 4.746257931590286E-5 9.492515863180572E-5 0.0 0.7557703817367791 9.492515863180572E-5 82-83 4.746257931590286E-5 9.492515863180572E-5 0.0 0.8362669162565504 9.492515863180572E-5 84-85 4.746257931590286E-5 9.492515863180572E-5 0.0 0.9182585220247726 9.492515863180572E-5 86-87 4.746257931590286E-5 9.492515863180572E-5 0.0 1.0064439943937202 9.492515863180572E-5 88-89 4.746257931590286E-5 9.492515863180572E-5 0.0 1.1066849619089068 9.492515863180572E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1675 0.0 49.83852 1 GTATCAA 3130 0.0 45.160328 1 ATCAACG 3795 0.0 36.89288 3 TCAACGC 3860 0.0 36.527393 4 CAACGCA 3920 0.0 35.833492 5 TATCAAC 3985 0.0 34.692356 2 AACGCAG 4285 0.0 33.43952 6 ACGCAGA 4805 0.0 28.600193 7 CGCAGAG 4890 0.0 28.156536 8 GCAGAGT 5315 0.0 24.308037 9 GTACATG 3370 0.0 24.011513 1 GTATAGG 430 0.0 22.629631 1 TACATGG 3420 0.0 22.307894 2 AGAGTAC 4790 0.0 20.810331 10-11 GTGGTAT 845 0.0 20.606989 1 GAGTACT 3635 0.0 20.273897 12-13 ACATGGG 3655 0.0 20.255339 3 CAGAGTA 5100 0.0 19.725117 10-11 GAGTACA 2655 0.0 18.904009 1 AGTACTT 3930 0.0 18.813147 12-13 >>END_MODULE