FastQCFastQC Report
Sat 18 Jun 2016
SRR1931019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106923
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58590.27808325221187485No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52830.2507448065259148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30550.14499817981008323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.057.2612651
GTATCAA56300.040.4281581
ATCAACG70500.032.0822143
CAACGCA72550.031.1877985
TCAACGC72550.031.1756864
TATCAAC73850.030.8206522
AACGCAG74950.030.3782716
GTGGTAT15450.026.450071
ACGCAGA86150.026.0428817
CGCAGAG88000.025.4953868
TGGTATC15100.024.5456472
GTACATG57750.023.0389611
GCAGAGT99700.022.0747439
TACATGG59800.021.8518562
GAGTACT71050.021.12917112-13
ACATGGG57600.021.036053
CAGAGTA97050.020.18493310-11
GAGTACA44500.019.6478331
AGAGTAC93000.019.63589110-11
GTACTTT77350.019.25493814-15
Produced by FastQC (version 0.11.5)