##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931019_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2106923 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36508595710427 31.0 31.0 34.0 30.0 34.0 2 31.523842589406446 31.0 31.0 34.0 30.0 34.0 3 31.585544417142913 31.0 31.0 34.0 30.0 34.0 4 35.35450607354896 37.0 35.0 37.0 33.0 37.0 5 35.234093035198725 37.0 35.0 37.0 33.0 37.0 6 35.26185579634377 37.0 35.0 37.0 32.0 37.0 7 35.14765228724543 37.0 35.0 37.0 32.0 37.0 8 35.15130738047855 37.0 35.0 37.0 32.0 37.0 9 36.656964207994314 39.0 35.0 39.0 32.0 39.0 10-11 36.59991656078556 39.0 35.0 39.0 32.0 39.0 12-13 36.48239707858332 38.0 35.0 39.0 32.0 39.0 14-15 37.54921893206349 39.0 36.0 41.0 32.0 41.0 16-17 37.50324382998335 39.0 36.0 41.0 32.0 41.0 18-19 37.52742340370294 39.0 36.0 41.0 32.0 41.0 20-21 37.51057584923606 39.0 36.0 41.0 32.0 41.0 22-23 37.40857757972171 39.0 36.0 41.0 32.0 41.0 24-25 37.27998175538451 39.0 36.0 41.0 31.0 41.0 26-27 37.0254138380947 39.0 36.0 40.0 31.0 41.0 28-29 36.838126262801254 39.0 36.0 40.0 30.5 41.0 30-31 36.60480378257772 38.5 35.0 40.0 30.0 41.0 32-33 36.43240877810912 38.0 35.0 40.0 30.0 41.0 34-35 36.510231033597336 38.0 35.0 40.0 30.0 41.0 36-37 36.580074592189646 38.5 35.0 40.0 30.0 41.0 38-39 36.56514476324004 39.0 35.0 40.0 30.0 41.0 40-41 36.44774227629581 39.0 35.0 40.0 30.0 41.0 42-43 36.30777346870293 39.0 35.0 40.0 30.0 41.0 44-45 36.14108583939708 38.0 35.0 40.0 30.0 41.0 46-47 35.88776879838513 38.0 35.0 40.0 28.5 41.0 48-49 35.66079064113876 38.0 35.0 40.0 28.0 41.0 50-51 35.3998776889331 38.0 34.0 40.0 27.0 41.0 52-53 35.18647857562901 38.0 34.0 40.0 26.5 41.0 54-55 35.03261058899637 38.0 34.0 40.0 26.0 41.0 56-57 34.85378037071122 38.0 34.0 40.0 26.0 41.0 58-59 34.63899084114607 38.0 33.5 40.0 26.0 41.0 60-61 34.327866039717634 37.5 33.0 40.0 25.5 41.0 62-63 34.06523423020205 37.0 33.0 40.0 25.0 41.0 64-65 33.748615397904906 37.0 32.0 39.5 24.0 41.0 66-67 33.42358002641767 36.0 32.0 39.0 23.0 41.0 68-69 33.06491456973036 36.0 31.5 39.0 22.5 40.0 70-71 32.67745024379154 35.0 31.0 38.5 22.0 40.0 72-73 32.243899753336976 35.0 31.0 38.0 21.5 40.0 74-75 31.72563567819042 35.0 31.0 37.0 20.0 39.0 76-77 30.346328983071523 33.5 28.5 36.0 19.5 38.5 78-79 31.072618695604916 34.0 30.0 36.0 20.0 39.0 80-81 31.021625375013706 34.0 30.0 36.0 20.0 38.5 82-83 30.749844916022084 34.0 30.0 35.5 19.5 37.0 84-85 30.417609945878418 34.0 30.0 35.0 18.0 37.0 86-87 30.067409914837896 34.0 30.0 35.0 17.5 36.5 88-89 29.71264991648959 34.0 29.5 35.0 12.0 36.0 90-91 29.370774109922387 34.0 29.0 35.0 7.0 36.0 92-93 29.043563765738 34.0 29.0 35.0 3.5 35.0 94-95 28.65790444169056 34.0 29.0 35.0 2.0 35.0 96-97 28.217282264230825 34.0 29.0 35.0 2.0 35.0 98-99 27.714647616452996 33.0 28.5 35.0 2.0 35.0 100-101 26.566736183524505 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3364225656338711 1101 2 0.4223330505155012 1101 3 0.293787857639515 1101 4 0.2650478526560178 1101 5 0.2782401527495466 1101 6 0.3373311265386434 1101 7 0.42247090888454863 1101 8 0.3958938630861155 1101 9 0.5126011418562797 1101 10-11 0.4429614559972421 1101 12-13 0.4131148567765166 1101 14-15 0.47932721945723245 1101 16-17 0.6032969735933804 1101 18-19 0.6218092768980554 1101 20-21 0.6331012032951904 1101 22-23 0.4603309499611967 1101 24-25 0.4026131648087343 1101 26-27 0.4658811922003636 1101 28-29 0.5575582595477755 1101 30-31 0.5499577021331561 1101 32-33 0.5539307782915941 1101 34-35 0.6745672560831437 1101 36-37 0.6186474852723265 1101 38-39 0.5338225754302215 1101 40-41 0.5459343870565263 1101 42-43 0.49305441908601466 1101 44-45 0.49722906021778357 1101 46-47 0.42677088206239944 1101 48-49 0.4500543874594527 1101 50-51 0.4242408758505576 1101 52-53 0.349041021093079 1101 54-55 0.4839251210299409 1101 56-57 0.4389762278185998 1101 58-59 0.5465022224291332 1101 60-61 0.47131934319899216 1101 62-63 0.34904069852709796 1101 64-65 0.4651277751309948 1101 66-67 0.2887718153285519 1101 68-69 0.30932064537569204 1101 70-71 0.1692230198503566 1101 72-73 0.23033084428190165 1101 74-75 0.28674970609997175 1101 76-77 0.22672094032999013 1101 78-79 0.2411116592714002 1101 80-81 0.19930039362320073 1101 82-83 0.20477443969401676 1101 84-85 0.23106429814362883 1101 86-87 0.22859815135658934 1101 88-89 0.28438079379164094 1101 90-91 0.2143489945338608 1101 92-93 0.04714926980842549 1101 94-95 0.35989396168785603 1101 96-97 0.5165842303744235 1101 98-99 0.5185522013323514 1101 100-101 0.6903004789517375 1102 1 0.3074559432387254 1102 2 0.3930285371621203 1102 3 0.35409512095693785 1102 4 0.1646039107732591 1102 5 0.30912037542302784 1102 6 0.2829097731682637 1102 7 0.4095390524580935 1102 8 0.4108045561640381 1102 9 0.5599489487432336 1102 10-11 0.5014768099631084 1102 12-13 0.43482372357053123 1102 14-15 0.5509090844145987 1102 16-17 0.5590788398889188 1102 18-19 0.5386303920720295 1102 20-21 0.5415879536205424 1102 22-23 0.493388645678678 1102 24-25 0.5545582960007494 1102 26-27 0.5508559591057889 1102 28-29 0.5669088063624059 1102 30-31 0.5359628273702697 1102 32-33 0.5722981627813937 1102 34-35 0.6512594708747343 1102 36-37 0.6499107625469875 1102 38-39 0.5593176609567792 1102 40-41 0.6862996438369322 1102 42-43 0.8825360575560737 1102 44-45 0.9004992006934458 1102 46-47 0.8358995415720258 1102 48-49 0.9534171970395064 1102 50-51 0.6880582324058082 1102 52-53 0.4795206673446657 1102 54-55 0.6132677307524972 1102 56-57 0.7442515085859114 1102 58-59 0.8640300231604456 1102 60-61 0.7143685330970655 1102 62-63 0.5433541358817848 1102 64-65 0.6949331449208671 1102 66-67 0.7440313724572079 1102 68-69 0.8472217236158528 1102 70-71 0.8137615226923245 1102 72-73 0.7897063983728927 1102 74-75 0.7711588401958487 1102 76-77 0.6825193248097534 1102 78-79 0.9642112735404318 1102 80-81 0.8864474481173161 1102 82-83 0.8150513011888343 1102 84-85 0.8593589440773997 1102 86-87 0.7158737131421269 1102 88-89 0.9489219813612664 1102 90-91 1.0404988692465729 1102 92-93 0.916462414435788 1102 94-95 0.9281719807101148 1102 96-97 0.9530076211269183 1102 98-99 1.2689350468425822 1102 100-101 1.3168119973534154 1103 1 0.22647463744711516 1103 2 0.30398290139745043 1103 3 0.2702955865808967 1103 4 0.21653648103730205 1103 5 0.19917112579648233 1103 6 0.2249367872721848 1103 7 0.25193754346713604 1103 8 0.1876199784737551 1103 9 0.3295100190188123 1103 10-11 0.3624318337224608 1103 12-13 0.2818172420982066 1103 14-15 0.2911415336559813 1103 16-17 0.34965976513516495 1103 18-19 0.41607598958430714 1103 20-21 0.4088505002406393 1103 22-23 0.35662685916000925 1103 24-25 0.28957349802944776 1103 26-27 0.19468969804639613 1103 28-29 0.19491417488794127 1103 30-31 0.310304210194289 1103 32-33 0.3208719420848709 1103 34-35 0.3175403159888148 1103 36-37 0.21682112333960646 1103 38-39 0.31740816578944475 1103 40-41 0.37543464510651603 1103 42-43 0.2525121333221918 1103 44-45 0.3845430474992 1103 46-47 0.23869518145872348 1103 48-49 0.290827419332075 1103 50-51 0.32714298618900983 1103 52-53 0.4288096592292234 1103 54-55 0.4452360554625585 1103 56-57 0.18967259818554538 1103 58-59 0.4327633495687806 1103 60-61 0.21847633724480886 1103 62-63 0.094564321241009 1103 64-65 0.17819421915359612 1103 66-67 0.11116860951322138 1103 68-69 0.1392272207485732 1103 70-71 0.09620783743697103 1103 72-73 0.3288182338612202 1103 74-75 0.3674800564240961 1103 76-77 0.46514494764775094 1103 78-79 0.6050555137522728 1103 80-81 0.5786156415820933 1103 82-83 0.6265611907515343 1103 84-85 0.5072087721065088 1103 86-87 0.5369769979015153 1103 88-89 0.5855753586189003 1103 90-91 0.561640117857447 1103 92-93 0.3523739920502251 1103 94-95 0.5707159379861473 1103 96-97 0.6745223664352302 1103 98-99 0.5968580160856796 1103 100-101 0.9539420868178432 1104 1 0.241096641443459 1104 2 0.31413803589755673 1104 3 0.3596875999154321 1104 4 0.12634278883619743 1104 5 0.30903828246727016 1104 6 0.32609191173121843 1104 7 0.3422543391615278 1104 8 0.28795535014388207 1104 9 0.4506573114448358 1104 10-11 0.3958016161325233 1104 12-13 0.4281564695384432 1104 14-15 0.5064756501926126 1104 16-17 0.5084351564857954 1104 18-19 0.4839063156177801 1104 20-21 0.44128114156365683 1104 22-23 0.4690569984296147 1104 24-25 0.2624070874100113 1104 26-27 0.4587519125035229 1104 28-29 0.46974433022326423 1104 30-31 0.4505961040401161 1104 32-33 0.46352167951094003 1104 34-35 0.5536754085172575 1104 36-37 0.5468994436697514 1104 38-39 0.4430869921894214 1104 40-41 0.45530250465206024 1104 42-43 0.4472350500516171 1104 44-45 0.6305347535770593 1104 46-47 0.5063234812046886 1104 48-49 0.4710003265224785 1104 50-51 0.3484080603422939 1104 52-53 0.2862590271439913 1104 54-55 0.5336071934167848 1104 56-57 0.4733949224919627 1104 58-59 0.4771987905222801 1104 60-61 0.22526751315427873 1104 62-63 0.442013387923744 1104 64-65 0.26182035002887716 1104 66-67 0.16281614794541355 1104 68-69 0.13456344590947822 1104 70-71 0.2592545986904966 1104 72-73 0.15196540579442086 1104 74-75 0.3887274582977547 1104 76-77 0.33421062627332887 1104 78-79 0.43916110562302535 1104 80-81 0.42284313201797374 1104 82-83 0.3103743867230584 1104 84-85 0.2764254683943257 1104 86-87 0.3261456978844457 1104 88-89 0.41298185117344843 1104 90-91 0.46286743759517535 1104 92-93 0.3921670456394075 1104 94-95 0.5500003110720684 1104 96-97 0.6542997559583803 1104 98-99 0.6293769183961295 1104 100-101 0.8495640807731242 1105 1 0.18884621168686522 1105 2 0.3572621794985409 1105 3 0.2364817402776751 1105 4 0.15153823466195604 1105 5 0.18891271880100646 1105 6 0.20899980679587316 1105 7 0.2588202725430264 1105 8 0.26098905800905214 1105 9 0.36036806657077847 1105 10-11 0.40654876977361454 1105 12-13 0.43345356214164354 1105 14-15 0.4177997987762794 1105 16-17 0.4072692081562437 1105 18-19 0.4446387703376331 1105 20-21 0.5196064610783395 1105 22-23 0.4135543700631885 1105 24-25 0.4992296290777176 1105 26-27 0.3477630986317237 1105 28-29 0.4461353713553251 1105 30-31 0.3556550242922327 1105 32-33 0.38327254525248833 1105 34-35 0.335233992438134 1105 36-37 0.42547838185954134 1105 38-39 0.552635810047363 1105 40-41 0.25288640061359047 1105 42-43 0.2208766455074027 1105 44-45 0.2917360789713115 1105 46-47 0.3414568926000783 1105 48-49 0.288148237531729 1105 50-51 0.1406564970248212 1105 52-53 0.0666905276796328 1105 54-55 0.2727940191972067 1105 56-57 0.3585350605123452 1105 58-59 0.41503281727930386 1105 60-61 0.27916621516478557 1105 62-63 0.31538093569523795 1105 64-65 0.1947673591721042 1105 66-67 0.29880060196768454 1105 68-69 0.20052373390867473 1105 70-71 0.10916216988061223 1105 72-73 0.14892596784131484 1105 74-75 0.324014834664883 1105 76-77 0.38252822262600716 1105 78-79 0.4079535518723105 1105 80-81 0.39427640140684517 1105 82-83 0.42263029743334 1105 84-85 0.46106783616631475 1105 86-87 0.4903351395745723 1105 88-89 0.49309196851568515 1105 90-91 0.40627858386189786 1105 92-93 0.6355507871376993 1105 94-95 0.5063485011116811 1105 96-97 0.5783402153619512 1105 98-99 0.7153117947009697 1105 100-101 0.8361217927240929 1106 1 0.19588882399131435 1106 2 0.184096899343281 1106 3 0.23714326133999464 1106 4 0.06971618719776984 1106 5 0.026806159790929485 1106 6 0.013701621766230687 1106 7 0.08179053581334017 1106 8 0.15379191898357192 1106 9 0.2347248577674108 1106 10-11 0.15811450508391545 1106 12-13 0.25845507370835463 1106 14-15 0.19360769401132671 1106 16-17 0.2125694053041869 1106 18-19 0.1505047394699588 1106 20-21 0.13768232091199906 1106 22-23 0.19379398064396014 1106 24-25 0.26768823742533243 1106 26-27 0.21779889054895563 1106 28-29 0.1973727249935422 1106 30-31 0.3272448059030637 1106 32-33 0.16875872953279014 1106 34-35 0.12500569134990513 1106 36-37 0.20956292254646058 1106 38-39 0.228609135107547 1106 40-41 0.22245524231736624 1106 42-43 0.0877914734316434 1106 44-45 0.16839957052189902 1106 46-47 0.10931424431538517 1106 48-49 -0.08046707815773857 1106 50-51 -0.017435439931446695 1106 52-53 -0.08993712239416851 1106 54-55 -0.08720348300076353 1106 56-57 -0.015743922168574898 1106 58-59 -0.020286103471981676 1106 60-61 5.543389090760797E-4 1106 62-63 -0.16264574122696018 1106 64-65 -0.09454165164873274 1106 66-67 -0.22857501096022048 1106 68-69 -0.08063718896969618 1106 70-71 -0.17971874542507038 1106 72-73 0.0346915689443108 1106 74-75 0.02106058904569963 1106 76-77 -0.049704227588936334 1106 78-79 -0.08732490845079965 1106 80-81 -0.05233218805857476 1106 82-83 0.1249272841127187 1106 84-85 0.11869074140744118 1106 86-87 0.07098203065355335 1106 88-89 0.05574417832709244 1106 90-91 0.0906532462373697 1106 92-93 0.08073751270756091 1106 94-95 0.16825067647918956 1106 96-97 0.2381551401255777 1106 98-99 0.2056009945816335 1106 100-101 0.359611043750224 1107 1 0.13095354766414857 1107 2 0.15149169905838988 1107 3 0.10744679523744338 1107 4 0.11800982138654348 1107 5 0.162370950557559 1107 6 0.22647449300728795 1107 7 0.21315600958681813 1107 8 0.16605122497175273 1107 9 0.11702408654612384 1107 10-11 0.1418768152064942 1107 12-13 0.23903588758381744 1107 14-15 0.25675644928255537 1107 16-17 0.17110554186108828 1107 18-19 0.2895930460147369 1107 20-21 0.3555401273341019 1107 22-23 0.24810114395818772 1107 24-25 0.2076030680115295 1107 26-27 0.24098912486906698 1107 28-29 0.18936494211381216 1107 30-31 0.3555770448268305 1107 32-33 0.42916749746053284 1107 34-35 0.16980469185092062 1107 36-37 0.2100770436058923 1107 38-39 0.21217195037888814 1107 40-41 0.1973746702177479 1107 42-43 0.20887164773257894 1107 44-45 0.2611777775058499 1107 46-47 0.1842778588365519 1107 48-49 0.230969991439558 1107 50-51 0.14397564570960952 1107 52-53 0.15332640295974187 1107 54-55 0.20231485901241797 1107 56-57 0.2008780086875035 1107 58-59 0.14417665788612055 1107 60-61 -0.01203825250720314 1107 62-63 -0.07714760754595318 1107 64-65 0.04188960183002166 1107 66-67 -0.07618010008380338 1107 68-69 -0.12932802633991258 1107 70-71 0.019595369441091748 1107 72-73 0.11521205795879297 1107 74-75 0.21080371510818452 1107 76-77 0.0661868970538606 1107 78-79 0.3479374243010547 1107 80-81 0.40389278575896626 1107 82-83 0.47477197588225906 1107 84-85 0.4042370516062519 1107 86-87 0.38712370494932813 1107 88-89 0.49265924155802665 1107 90-91 0.5000801024922943 1107 92-93 0.36574488905450053 1107 94-95 0.5060654317138962 1107 96-97 0.6397305340931716 1107 98-99 0.6161241792578842 1107 100-101 0.8137376493363995 1108 1 0.04729442018541263 1108 2 0.1187963940417518 1108 3 0.07119495340666049 1108 4 0.13777703131938068 1108 5 0.12402972957649183 1108 6 0.04961320917732337 1108 7 0.1095863858209114 1108 8 0.22938674997531905 1108 9 0.11802137533967283 1108 10-11 0.1362303079641336 1108 12-13 0.09802565768707439 1108 14-15 0.10061689507459448 1108 16-17 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-2.2156034361043666 2316 78-79 -2.0003580040396116 2316 80-81 -1.7130653863872176 2316 82-83 -1.289320627188463 2316 84-85 -1.1787798599673884 2316 86-87 -1.1526105907636115 2316 88-89 -1.2707380398114587 2316 90-91 -1.3321783530133011 2316 92-93 -1.1323505341116835 2316 94-95 -1.6011945142689576 2316 96-97 -1.5646459570871407 2316 98-99 -1.6098865892996805 2316 100-101 -1.875836293521516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 28.0 5 46.0 6 104.0 7 281.0 8 730.0 9 1319.0 10 2334.0 11 3893.0 12 5354.0 13 7120.0 14 8780.0 15 11338.0 16 13554.0 17 15685.0 18 13669.0 19 11217.0 20 10918.0 21 11465.0 22 12710.0 23 14442.0 24 17789.0 25 22165.0 26 28905.0 27 36902.0 28 47080.0 29 58309.0 30 71441.0 31 85745.0 32 103366.0 33 126287.0 34 156058.0 35 195530.0 36 257383.0 37 354433.0 38 338395.0 39 62117.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.60495511274262 21.417776625970532 14.93045963754145 23.04680862374539 2 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20.311183655026785 20-21 21.19897120113075 27.774603058583537 30.533816375823893 20.492609364461824 22-23 20.96503655819053 27.638609056369145 30.450611438999676 20.94574294644065 24-25 20.94134108307478 27.87265610427408 30.39725493602239 20.78874787662875 26-27 20.819440258393396 28.10006811067629 30.305572497525944 20.77491913340437 28-29 20.812062157082377 28.19948075864295 30.30193362823458 20.686523456040096 30-31 21.084263435104415 27.848135813330476 30.302541266900306 20.765059484664803 32-33 20.91433362101787 28.039534311840548 30.167165604702458 20.878966462439127 34-35 21.074372221155496 28.02781757892316 30.232238002120425 20.66557219780092 36-37 21.080121305522326 27.872646762808973 30.32196653727506 20.72526539439364 38-39 20.91602908511002 28.042569248001826 30.308507204829798 20.73289446205836 40-41 21.064111436076043 27.97815172476641 30.255888525953374 20.701848313204177 42-43 21.01226520643037 28.075023816971733 29.95878370392241 20.95392727267549 44-45 21.010006030531432 28.30433844642973 29.870045726190142 20.815609796848697 46-47 21.164175746350484 28.316224573008824 29.59121723932676 20.928382441313932 48-49 21.114823852078324 28.428100789406418 29.437186285057336 21.01988907345792 50-51 21.207874585635096 28.56907809485586 29.22721153521513 20.995835784293913 52-53 21.254710122546342 28.52799464008249 29.2085331027175 21.008762134653672 54-55 21.1397501242543 28.575212656421563 29.285349101167917 20.99968811815622 56-57 21.122968000637936 28.678792211096304 29.19500358839411 21.00323619987165 58-59 21.11026744844843 28.577647254012533 29.32849383745171 20.983591460087332 60-61 21.064733302254275 28.732872659982295 29.303558266815124 20.898835770948306 62-63 21.059552740704934 28.64991997594521 29.34044859144342 20.950078691906437 64-65 20.90313411527814 28.505363807024274 29.341295997027206 21.250206080670385 66-67 20.870941247903886 28.697416331510517 29.240121855353397 21.191520565232207 68-69 20.985044024759215 28.673680524114587 29.265895556317407 21.075379894808794 70-71 20.96510189309034 28.584753038095638 29.172664120915336 21.277480947898685 72-73 21.01864363873627 28.719213988849706 29.11298018927685 21.149162183137175 74-75 20.984756547355595 28.825378468801606 29.007319522374992 21.182545461467807 76-77 20.89863239391469 28.794804866193008 29.004768595190694 21.301794144701606 78-79 20.90880374556172 28.81180512655724 28.997393456990856 21.281997670890185 80-81 20.950931761625842 29.00573490345874 28.793933143261523 21.249400191653894 82-83 21.02127130417201 28.859977322379603 28.982810477649156 21.13594089579923 84-85 21.054400184534508 28.950654580162638 28.750647270925423 21.244297964377434 86-87 21.00112343925241 28.989834939387915 28.785271222536373 21.22377039882331 88-89 20.92289130779402 28.877860689669575 28.775626245300018 21.423621757236386 90-91 20.90324013797274 28.914468578545687 28.91643839712777 21.26585288635381 92-93 20.919000094705083 28.807530074798027 28.933067716033804 21.34040211446308 94-95 20.765098129117785 28.77549935520372 28.95282426465458 21.506578251023914 96-97 20.873079307159923 28.643514093070365 28.974542009623516 21.508864590146196 98-99 20.65991495655038 28.775944825700794 28.844219746046722 21.719920471702096 100-101 20.68877220477445 28.705154388651128 28.8637031348559 21.74237027171852 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 233.0 1 170.5 2 172.5 3 268.0 4 236.5 5 216.5 6 400.5 7 888.5 8 1533.5 9 1920.0 10 1726.5 11 1411.0 12 1430.0 13 1580.5 14 1781.5 15 2041.5 16 2291.5 17 2528.0 18 2854.5 19 3215.5 20 3632.0 21 4262.0 22 5212.0 23 6498.0 24 8736.5 25 11545.0 26 14322.5 27 17838.5 28 22082.5 29 27548.0 30 33626.5 31 39458.0 32 45747.0 33 51163.0 34 55758.5 35 59856.5 36 65113.0 37 71684.0 38 75875.0 39 77776.0 40 79271.0 41 81316.5 42 82789.5 43 84133.0 44 85776.0 45 87736.0 46 88952.0 47 88135.5 48 86286.5 49 84105.5 50 80095.5 51 74464.5 52 68611.0 53 62204.0 54 55353.0 55 47137.5 56 38289.5 57 30114.0 58 23201.5 59 17432.0 60 12557.0 61 8876.0 62 6352.5 63 4113.0 64 2263.5 65 1234.5 66 691.5 67 398.0 68 205.5 69 121.0 70 67.5 71 39.0 72 29.0 73 16.5 74 8.0 75 6.5 76 7.0 77 6.5 78 3.0 79 0.5 80 0.5 81 1.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0042241695591153545 2 0.006265060469699177 3 0.0014713399587929887 4 0.17333333966167724 5 0.0015188025381088915 6 4.746257931590286E-4 7 9.492515863180572E-5 8 6.170135311067372E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.797006345272229E-4 24-25 6.644761104226401E-4 26-27 0.002752829600322366 28-29 1.4238773794770859E-4 30-31 0.005671778228250391 32-33 0.021310698112840386 34-35 0.01279116512563582 36-37 5.458196621328829E-4 38-39 1.4238773794770859E-4 40-41 0.001376414800161183 42-43 0.011011318401289464 44-45 0.006810880131832061 46-47 0.009729828759760086 48-49 0.01013326068394526 50-51 0.19013509273950685 52-53 0.1145272038892736 54-55 0.016896678236461418 56-57 0.006217597890383274 58-59 0.0174424978985943 60-61 0.12397225717313827 62-63 0.23992333844188896 64-65 0.2466867559944051 66-67 0.257009866995614 68-69 0.25257211582957706 70-71 0.2368145394966973 72-73 0.16092187517056863 74-75 0.27020446404543497 76-77 0.20964221283834295 78-79 0.005197152435091363 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 4.746257931590286E-5 90-91 0.006170135311067372 92-93 0.018201899167648746 94-95 0.0026579044416905604 96-97 4.746257931590286E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2106923.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.4146118753812 #Duplication Level Percentage of deduplicated Percentage of total 1 76.93804549145766 47.25120202308295 2 14.090233074810898 17.30692391046341 3 4.018310736702438 7.40348982867772 4 1.7162452017399001 4.216101317913651 5 0.9253560037721683 2.8415189909110747 6 0.5445315085431629 2.00653147506565 7 0.35524193370115886 1.52719318360816 8 0.2500300160371624 1.2284397113694132 9 0.18410560971383064 1.0176097108189788 >10 0.9100346796945041 9.836099969354832 >50 0.045347295853198584 1.9022647432230058 >100 0.021121872178365114 2.298199907393351 >500 0.0010862256187520567 0.42785015031592205 >1k 1.5517508839313723E-4 0.20565778469355 >5k 1.5517508839313723E-4 0.5309172931083258 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5859 0.27808325221187485 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5283 0.2507448065259148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3055 0.14499817981008323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.746257931590286E-5 0.0 4 0.0 0.0 0.0 4.746257931590286E-5 0.0 5 0.0 0.0 0.0 9.492515863180572E-5 0.0 6 0.0 0.0 0.0 9.492515863180572E-5 0.0 7 0.0 0.0 0.0 9.492515863180572E-5 0.0 8 0.0 0.0 0.0 9.492515863180572E-5 0.0 9 0.0 0.0 0.0 9.492515863180572E-5 0.0 10-11 0.0 0.0 0.0 9.492515863180572E-5 0.0 12-13 0.0 0.0 0.0 1.4238773794770859E-4 0.0 14-15 0.0 0.0 0.0 1.4238773794770859E-4 0.0 16-17 0.0 0.0 0.0 1.4238773794770859E-4 0.0 18-19 0.0 0.0 0.0 1.4238773794770859E-4 0.0 20-21 0.0 0.0 0.0 1.8985031726361144E-4 0.0 22-23 0.0 0.0 0.0 1.8985031726361144E-4 0.0 24-25 0.0 0.0 0.0 2.6104418623746576E-4 0.0 26-27 0.0 0.0 0.0 3.797006345272229E-4 0.0 28-29 0.0 0.0 0.0 6.6447611042264E-4 0.0 30-31 0.0 0.0 0.0 0.0025629792830587543 0.0 32-33 0.0 0.0 0.0 0.0070244617387536235 0.0 34-35 0.0 0.0 0.0 0.013598028974006169 0.0 36-37 0.0 0.0 0.0 0.025083973168454662 0.0 38-39 0.0 0.0 0.0 0.04326214104644546 0.0 40-41 0.0 0.0 0.0 0.06262687340733382 0.0 42-43 0.0 0.0 0.0 0.08308324509248796 0.0 44-45 0.0 0.0 0.0 0.10802483052299491 0.0 46-47 0.0 0.0 0.0 0.13935013287149078 0.0 48-49 0.0 0.0 0.0 0.17236035678570122 0.0 50-51 0.0 0.0 0.0 0.2076962470863909 0.0 52-53 0.0 0.0 0.0 0.2430795999663965 4.746257931590286E-5 54-55 0.0 0.0 0.0 0.2831380169090185 9.492515863180572E-5 56-57 0.0 0.0 0.0 0.3316210416802133 9.492515863180572E-5 58-59 0.0 0.0 0.0 0.3854673379141051 9.492515863180572E-5 60-61 0.0 0.0 0.0 0.44318183436224295 9.492515863180572E-5 62-63 0.0 0.0 0.0 0.5035542352520714 1.4238773794770859E-4 64-65 0.0 0.0 0.0 0.5668218534801699 1.4238773794770859E-4 66-67 0.0 0.0 0.0 0.6416703410613487 1.4238773794770859E-4 68-69 0.0 0.0 0.0 0.7222618007397518 1.4238773794770859E-4 70-71 0.0 0.0 0.0 0.8206991902409343 1.4238773794770859E-4 72-73 0.0 0.0 0.0 0.9224352290045721 1.4238773794770859E-4 74-75 0.0 0.0 0.0 1.0243848493751315 1.4238773794770859E-4 76-77 0.0 0.0 0.0 1.1356608665812655 1.4238773794770859E-4 78-79 0.0 0.0 0.0 1.2668711671000792 1.4238773794770859E-4 80-81 0.0 0.0 0.0 1.4159985913106459 1.4238773794770859E-4 82-83 0.0 0.0 0.0 1.5675466070663235 1.4238773794770859E-4 84-85 0.0 0.0 0.0 1.7235323739880384 1.4238773794770859E-4 86-87 0.0 0.0 0.0 1.8915024421870186 1.4238773794770859E-4 88-89 0.0 0.0 0.0 2.0842479767889004 1.4238773794770859E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3170 0.0 57.261265 1 GTATCAA 5630 0.0 40.428158 1 ATCAACG 7050 0.0 32.082214 3 CAACGCA 7255 0.0 31.187798 5 TCAACGC 7255 0.0 31.175686 4 TATCAAC 7385 0.0 30.820652 2 AACGCAG 7495 0.0 30.378271 6 GTGGTAT 1545 0.0 26.45007 1 ACGCAGA 8615 0.0 26.042881 7 CGCAGAG 8800 0.0 25.495386 8 TGGTATC 1510 0.0 24.545647 2 GTACATG 5775 0.0 23.038961 1 GCAGAGT 9970 0.0 22.074743 9 TACATGG 5980 0.0 21.851856 2 GAGTACT 7105 0.0 21.129171 12-13 ACATGGG 5760 0.0 21.03605 3 CAGAGTA 9705 0.0 20.184933 10-11 GAGTACA 4450 0.0 19.647833 1 AGAGTAC 9300 0.0 19.635891 10-11 GTACTTT 7735 0.0 19.254938 14-15 >>END_MODULE