FastQCFastQC Report
Sat 18 Jun 2016
SRR1931018_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931018_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205254
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN538354.466693327713495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11450.064.005991
GTATCAA22450.044.9717671
ATCAACG27850.034.56783
TATCAAC29000.033.2217372
TCAACGC29550.032.9161534
CAACGCA29150.032.6681065
AACGCAG31100.030.5708436
GTGGTAT5650.029.0262971
TGGTATC4950.028.9878042
CGCAGAG36000.026.7490088
ACGCAGA35900.026.3490497
GTACATG21700.024.7981741
GCAGAGT38500.024.6605579
TACATGG21850.024.3918322
ACATGGG22450.022.9006253
GTACTAG2703.8744474E-1022.777581
AGAGTAC34200.021.78040710-11
CAGAGTA36400.020.71587210-11
GAGTACT26000.020.60568412-13
AGTACTT27950.020.54336712-13
Produced by FastQC (version 0.11.5)