FastQCFastQC Report
Sat 18 Jun 2016
SRR1931018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205254
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44630.37029539001737394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40860.3390156763636545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.22783579228942613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.061.5812951
GTATCAA40200.038.7727281
GTGGTAT9150.031.1608091
ATCAACG52750.028.9163253
TATCAAC53200.028.6717322
CAACGCA53750.028.3359645
TCAACGC54400.028.0392674
TGGTATC10200.027.4860522
AACGCAG55950.027.3913766
GTGTACG700.00837430927.154421
ACGCAGA62350.024.4275557
CGCAGAG62950.024.1947298
GAGTACT49100.022.12918512-13
GCAGAGT68650.021.563819
CAGAGTA68050.021.02183310-11
CATTGCG1150.003747407820.6292579
GTACATG37850.020.464461
AGAGTAC66050.020.2216710-11
GTACTTT53850.020.1331614-15
ACATGGG36650.019.837093
Produced by FastQC (version 0.11.5)