##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931018_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1205254 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.351770664108976 31.0 31.0 34.0 30.0 34.0 2 31.51762947893141 31.0 31.0 34.0 30.0 34.0 3 31.562592615332537 31.0 31.0 34.0 30.0 34.0 4 35.33922144211925 37.0 35.0 37.0 33.0 37.0 5 35.215372029464326 37.0 35.0 37.0 33.0 37.0 6 35.249697574121306 37.0 35.0 37.0 32.0 37.0 7 35.13449198260284 37.0 35.0 37.0 32.0 37.0 8 35.13848865052512 37.0 35.0 37.0 32.0 37.0 9 36.6359099409751 39.0 35.0 39.0 32.0 39.0 10-11 36.58287049866667 38.5 35.0 39.0 32.0 39.0 12-13 36.461733377362776 38.0 35.0 39.0 32.0 39.0 14-15 37.50584524092017 39.0 36.0 41.0 32.0 41.0 16-17 37.462720721109406 39.0 36.0 41.0 32.0 41.0 18-19 37.51101012732586 39.0 36.0 41.0 32.0 41.0 20-21 37.511642359203954 39.0 36.0 41.0 32.0 41.0 22-23 37.410148815104534 39.0 36.0 41.0 32.0 41.0 24-25 37.282436316328344 39.0 36.0 41.0 31.0 41.0 26-27 37.022036848664264 39.0 36.0 40.0 31.0 41.0 28-29 36.8371227143822 39.0 36.0 40.0 30.5 41.0 30-31 36.59877378544274 38.5 35.0 40.0 30.0 41.0 32-33 36.40516770738782 38.0 35.0 40.0 30.0 41.0 34-35 36.46466387997883 38.0 35.0 40.0 30.0 41.0 36-37 36.516475780208985 38.0 35.0 40.0 30.0 41.0 38-39 36.49222985362422 39.0 35.0 40.0 30.0 41.0 40-41 36.366421517787956 39.0 35.0 40.0 30.0 41.0 42-43 36.208486343957375 38.0 35.0 40.0 30.0 41.0 44-45 36.00935114092134 38.0 35.0 40.0 29.5 41.0 46-47 35.72722720687921 38.0 35.0 40.0 28.5 41.0 48-49 35.48172750308234 38.0 34.5 40.0 27.0 41.0 50-51 35.20163384647552 38.0 34.0 40.0 26.5 41.0 52-53 34.99705248852109 38.0 34.0 40.0 26.0 41.0 54-55 34.84219467431761 38.0 34.0 40.0 26.0 41.0 56-57 34.66600193818067 38.0 33.5 40.0 26.0 41.0 58-59 34.453954519130406 38.0 33.0 40.0 25.0 41.0 60-61 34.14166308512563 37.0 33.0 40.0 24.5 41.0 62-63 33.89045711526367 37.0 33.0 40.0 23.5 41.0 64-65 33.57830548581461 36.5 32.0 39.5 23.0 41.0 66-67 33.25811405728585 36.0 32.0 39.0 22.5 41.0 68-69 32.91367711702264 36.0 31.5 39.0 22.0 40.0 70-71 32.536320144965295 35.0 31.0 39.0 22.0 40.0 72-73 32.118892366256404 35.0 31.0 38.0 20.5 40.0 74-75 31.611257875933205 35.0 30.5 37.0 19.0 39.5 76-77 30.24201330176046 33.5 28.5 36.0 18.0 38.5 78-79 30.967359992167623 34.0 30.0 36.0 19.0 39.0 80-81 30.93836071068837 34.0 30.0 36.0 19.0 38.5 82-83 30.665341911331552 34.0 30.0 36.0 18.0 37.0 84-85 30.323680319667055 34.0 30.0 35.0 17.5 37.0 86-87 29.977853630852916 34.0 30.0 35.0 13.5 36.5 88-89 29.616324027964232 34.0 29.0 35.0 8.5 36.0 90-91 29.277734402872753 34.0 29.0 35.0 6.0 36.0 92-93 28.95602254794425 34.0 29.0 35.0 2.0 35.5 94-95 28.56676559463814 34.0 29.0 35.0 2.0 35.0 96-97 28.14211983532102 34.0 29.0 35.0 2.0 35.0 98-99 27.651948883803747 33.0 28.0 35.0 2.0 35.0 100-101 26.51038619245404 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2985030481236066 1101 2 0.3851446633927864 1101 3 0.25568604625965463 1101 4 0.2759563166827306 1101 5 0.34512504019395607 1101 6 0.190798696449896 1101 7 0.4159623607740741 1101 8 0.475575466743031 1101 9 0.42880479720970044 1101 10-11 0.5352047267582236 1101 12-13 0.5456492015769427 1101 14-15 0.6184948027304316 1101 16-17 0.6775276675349744 1101 18-19 0.5206462086826633 1101 20-21 0.5057642606456199 1101 22-23 0.5055218493885363 1101 24-25 0.5032055781305687 1101 26-27 0.649616866180402 1101 28-29 0.5726200455651664 1101 30-31 0.49814557477914434 1101 32-33 0.5399389943315356 1101 34-35 0.612454118157558 1101 36-37 0.5083713519957413 1101 38-39 0.7269478664502316 1101 40-41 0.5553202602268144 1101 42-43 0.5895174938372847 1101 44-45 0.5525250943749853 1101 46-47 0.6681110586185355 1101 48-49 0.685828039755485 1101 50-51 0.7947813834088748 1101 52-53 0.6725969632787283 1101 54-55 0.7178863670413165 1101 56-57 0.6560930174521147 1101 58-59 0.906122655881596 1101 60-61 0.7838488744640202 1101 62-63 0.6256342014610823 1101 64-65 0.6814299636386281 1101 66-67 0.7715118673013563 1101 68-69 0.6221780063633418 1101 70-71 0.7193975152813366 1101 72-73 0.5502933748306518 1101 74-75 0.6652832115055851 1101 76-77 0.7339278325474652 1101 78-79 0.6886101448100419 1101 80-81 0.7129605460880342 1101 82-83 0.8125353948022891 1101 84-85 0.7209938996730578 1101 86-87 0.6659439272476533 1101 88-89 0.7887881303994817 1101 90-91 0.5787386317472176 1101 92-93 0.6805544137577257 1101 94-95 0.7455577113486633 1101 96-97 1.1057125394175742 1101 98-99 1.01143555908331 1101 100-101 1.0564854484348096 1102 1 0.3154974466326905 1102 2 0.40062493155740597 1102 3 0.27539084359963795 1102 4 0.09729684960912977 1102 5 0.18588086557305417 1102 6 0.29523873860286187 1102 7 0.31299424324168257 1102 8 0.3115917212224488 1102 9 0.5555525428305259 1102 10-11 0.40587484813924846 1102 12-13 0.3502142781869182 1102 14-15 0.6074477842350987 1102 16-17 0.6902517040904002 1102 18-19 0.45275236662966734 1102 20-21 0.3318265589319367 1102 22-23 0.42395796904704497 1102 24-25 0.4339598339843036 1102 26-27 0.28949982443234745 1102 28-29 0.3744163607416837 1102 30-31 0.4261323881282877 1102 32-33 0.5411632900564882 1102 34-35 0.39736451672466444 1102 36-37 0.4812175741218283 1102 38-39 0.46605153557845824 1102 40-41 0.5641976407285654 1102 42-43 0.4289313090896414 1102 44-45 0.4191827043967393 1102 46-47 0.3406173927744405 1102 48-49 0.5178014475115731 1102 50-51 0.31782636811589526 1102 52-53 0.15186999606333984 1102 54-55 0.04316390264909842 1102 56-57 -0.051052345655598685 1102 58-59 -0.057778260905855916 1102 60-61 0.08702017298906384 1102 62-63 -0.02719833018331741 1102 64-65 -0.11625162319108284 1102 66-67 0.04740343024525373 1102 68-69 0.10336447520825942 1102 70-71 0.2282781931052611 1102 72-73 0.21947897582037257 1102 74-75 0.06283541141299764 1102 76-77 0.03129881162793424 1102 78-79 -0.02154171666022009 1102 80-81 0.07764833584021602 1102 82-83 -0.10429328029409035 1102 84-85 -0.06904944032965687 1102 86-87 0.04519302147324922 1102 88-89 0.16344603322344042 1102 90-91 -0.014604602345510642 1102 92-93 -0.049068464910050125 1102 94-95 0.045120090749104946 1102 96-97 0.19703814206915027 1102 98-99 0.3898817553583882 1102 100-101 0.6884970391817333 1103 1 0.2458288479529287 1103 2 0.2774771609422402 1103 3 0.16016295035411332 1103 4 0.09959358824027476 1103 5 0.11499164063202016 1103 6 0.17554123714482017 1103 7 0.29438420881641036 1103 8 0.2190408962029622 1103 9 0.4464910057188476 1103 10-11 0.2820193465470169 1103 12-13 0.2634859436579049 1103 14-15 0.4215177599257913 1103 16-17 0.3669212266268289 1103 18-19 0.26095598154144284 1103 20-21 0.3805948360682976 1103 22-23 0.23677054485231963 1103 24-25 0.3132991216340386 1103 26-27 0.28995788185597604 1103 28-29 0.25335049337446947 1103 30-31 0.200685556337298 1103 32-33 0.2560851235248691 1103 34-35 0.1262293601077431 1103 36-37 0.19381299199922353 1103 38-39 0.10104492922913977 1103 40-41 0.07374394452773458 1103 42-43 0.12167348598497085 1103 44-45 0.106386257373984 1103 46-47 -0.02381825467359988 1103 48-49 0.11506768338921347 1103 50-51 -0.07359982915588859 1103 52-53 0.08621552877767868 1103 54-55 0.0180855812811771 1103 56-57 0.2690826974146958 1103 58-59 0.2298405114486357 1103 60-61 0.002603415571108769 1103 62-63 -0.2878426814965849 1103 64-65 -0.26379966115694486 1103 66-67 0.03947516590870492 1103 68-69 -0.15824179669462524 1103 70-71 -0.17563619789771678 1103 72-73 -0.11476810680543537 1103 74-75 -0.05220345250137015 1103 76-77 0.09846809416807645 1103 78-79 0.06962719427277264 1103 80-81 0.19987224979252005 1103 82-83 0.08471218026651783 1103 84-85 0.15282841446484952 1103 86-87 0.14007348784022255 1103 88-89 0.21332913180772195 1103 90-91 -0.02064192821917743 1103 92-93 0.010248035995740423 1103 94-95 0.24222800649750553 1103 96-97 0.24862960129816614 1103 98-99 0.3175861005814298 1103 100-101 0.5831135096363873 1104 1 0.18423111863030783 1104 2 0.3574922953942661 1104 3 0.3076792695738497 1104 4 0.13148294877145617 1104 5 0.19894708317460896 1104 6 0.19795627783458514 1104 7 0.3480236113877382 1104 8 0.26410028746167313 1104 9 0.38231990997432774 1104 10-11 0.5291790889046268 1104 12-13 0.44737781640621677 1104 14-15 0.6402335226868914 1104 16-17 0.5636945818380212 1104 18-19 0.4703891529989832 1104 20-21 0.3220469281387537 1104 22-23 0.4603202872608847 1104 24-25 0.4136075434215414 1104 26-27 0.38335197381433517 1104 28-29 0.528812170597881 1104 30-31 0.5280712708414015 1104 32-33 0.5317321595672837 1104 34-35 0.5604683825342462 1104 36-37 0.6133494293390243 1104 38-39 0.50134927439656 1104 40-41 0.4176011150476526 1104 42-43 0.39313880159179604 1104 44-45 0.6699356685571729 1104 46-47 0.4621416110017691 1104 48-49 0.5768398156143846 1104 50-51 0.4465692486616888 1104 52-53 0.29203898055380506 1104 54-55 0.47349627527478333 1104 56-57 0.6633026871212309 1104 58-59 0.7347082506060971 1104 60-61 0.4282369478095305 1104 62-63 0.38162305196379975 1104 64-65 0.5579940771546248 1104 66-67 0.5699090456789833 1104 68-69 0.6465543468227892 1104 70-71 0.5656442939464981 1104 72-73 0.5518299792214307 1104 74-75 0.5971989404746161 1104 76-77 0.8732412047820652 1104 78-79 0.9496841140805614 1104 80-81 0.6930426372242984 1104 82-83 0.8755146884070193 1104 84-85 0.900737872733 1104 86-87 0.8785920695840979 1104 88-89 0.8203900925651588 1104 90-91 0.5302317424204972 1104 92-93 0.5794435659818298 1104 94-95 0.6785448312181295 1104 96-97 0.8016829897338162 1104 98-99 0.6378899616502167 1104 100-101 0.8721554274822978 1105 1 0.33652106579161867 1105 2 0.32911351029699887 1105 3 0.45580868502632654 1105 4 0.1998522925901014 1105 5 0.199782561401598 1105 6 0.2340822814387522 1105 7 0.22728514354740526 1105 8 0.1303719440189255 1105 9 0.21531168382323784 1105 10-11 0.29387863519873036 1105 12-13 0.3730555341144779 1105 14-15 0.43854701809549823 1105 16-17 0.4548819724859072 1105 18-19 0.3582599198780798 1105 20-21 0.2375168877635474 1105 22-23 0.4319589860129156 1105 24-25 0.34406397179582626 1105 26-27 0.3150688003957782 1105 28-29 0.3984260522746794 1105 30-31 0.32486554463098827 1105 32-33 0.3741642754002896 1105 34-35 0.44768725160394496 1105 36-37 0.5390268348356955 1105 38-39 0.6270322372596127 1105 40-41 0.438453415235351 1105 42-43 0.6081120950120038 1105 44-45 0.8004797659472089 1105 46-47 0.5564301369758979 1105 48-49 0.4803776725190758 1105 50-51 0.6344088568236543 1105 52-53 0.8376484113421157 1105 54-55 0.7923920078418263 1105 56-57 0.9770391230885096 1105 58-59 0.8888930099346695 1105 60-61 0.9916034130372751 1105 62-63 0.6534778635815712 1105 64-65 0.8117012776281811 1105 66-67 0.8528973227580323 1105 68-69 0.7864135893474966 1105 70-71 0.5954171038133822 1105 72-73 0.49803592148227693 1105 74-75 0.5403736203640683 1105 76-77 0.5825803277672108 1105 78-79 0.604532010835932 1105 80-81 0.5283691293771753 1105 82-83 0.6640974850486252 1105 84-85 0.6473141603872783 1105 86-87 0.6465642178121733 1105 88-89 0.5157883434309198 1105 90-91 0.5022354165265419 1105 92-93 0.6979119433182639 1105 94-95 0.6567624526902947 1105 96-97 0.4887501864108863 1105 98-99 0.5307498698100552 1105 100-101 0.6970602966094397 1106 1 0.17601159346996553 1106 2 0.24305236077290004 1106 3 0.19792414137508985 1106 4 0.03549658091230867 1106 5 -0.0015854844272027435 1106 6 0.16793104048927177 1106 7 0.15489333601252753 1106 8 0.23165315920329732 1106 9 0.2836781560781887 1106 10-11 0.2672727005773936 1106 12-13 0.29994054959083627 1106 14-15 0.3037285472535558 1106 16-17 0.28339175853421494 1106 18-19 0.3539440169011314 1106 20-21 0.222879810213783 1106 22-23 0.22326622568773047 1106 24-25 0.31525206945128303 1106 26-27 0.41563826367617196 1106 28-29 0.36860843368230434 1106 30-31 0.5576667777990494 1106 32-33 0.5357233115924913 1106 34-35 0.42156672869325007 1106 36-37 0.4937328581404046 1106 38-39 0.45823278516962773 1106 40-41 0.29920232027993166 1106 42-43 0.1996452425796278 1106 44-45 0.28303983719033 1106 46-47 0.1199035850788377 1106 48-49 0.10950367205959566 1106 50-51 -0.08576858400675746 1106 52-53 0.0101085223568802 1106 54-55 0.16656631387600385 1106 56-57 0.40488816869250144 1106 58-59 0.19812589886307563 1106 60-61 0.22629657848980145 1106 62-63 -0.012603035899914516 1106 64-65 0.059098211011907154 1106 66-67 -0.007109569347171885 1106 68-69 -0.11875697172914812 1106 70-71 -0.20154740555025086 1106 72-73 -0.0336161951845142 1106 74-75 0.18088023950238252 1106 76-77 0.14620569861928345 1106 78-79 0.03550226609085172 1106 80-81 0.1640917258481167 1106 82-83 0.0035892478712717946 1106 84-85 0.11453929755005277 1106 86-87 0.30187836441392335 1106 88-89 0.28192673887965825 1106 90-91 0.21670926975681581 1106 92-93 0.06351311275289007 1106 94-95 0.24023473975759302 1106 96-97 0.20628843265419405 1106 98-99 0.025882474902310548 1106 100-101 0.12851009663958024 1107 1 0.09005710588479587 1107 2 0.1057652769706614 1107 3 0.111712319770362 1107 4 0.0701394037556895 1107 5 0.14425764770950877 1107 6 0.03773664864713311 1107 7 0.08325105246245101 1107 8 0.22305955733965277 1107 9 0.024978665574920456 1107 10-11 -0.030865690949553937 1107 12-13 0.044060036301814876 1107 14-15 0.0071012235519845035 1107 16-17 0.007032567030918813 1107 18-19 0.08572840054306141 1107 20-21 0.12992990046537756 1107 22-23 0.2062640192639762 1107 24-25 0.008292907990799847 1107 26-27 0.03522439915945341 1107 28-29 -0.13801083755760146 1107 30-31 0.20924636542437014 1107 32-33 0.06186456950027264 1107 34-35 0.06503444620206267 1107 36-37 0.03260999530559161 1107 38-39 0.09913947511537913 1107 40-41 0.10165198008189691 1107 42-43 0.143258002899465 1107 44-45 0.061055077766859256 1107 46-47 0.07163252493655392 1107 48-49 -0.07920496672962685 1107 50-51 0.028833090018757446 1107 52-53 0.05807669173930918 1107 54-55 0.16937390190516766 1107 56-57 0.2970639832347146 1107 58-59 0.1949557038063574 1107 60-61 0.09561799237401658 1107 62-63 -0.04348958237886791 1107 64-65 -0.1000130139226485 1107 66-67 -0.18323500552217808 1107 68-69 -0.08582191101555736 1107 70-71 -0.11552519848624598 1107 72-73 -0.08641614938152742 1107 74-75 0.1470558989054176 1107 76-77 0.3490199970331709 1107 78-79 0.10584187312087323 1107 80-81 0.1957721293084589 1107 82-83 0.13281771369263495 1107 84-85 0.3378895279194083 1107 86-87 0.21728842432719375 1107 88-89 0.18113121969288315 1107 90-91 0.40754060639157785 1107 92-93 0.1731143188983708 1107 94-95 0.199691703413162 1107 96-97 0.2511017977705343 1107 98-99 0.34403197242377814 1107 100-101 0.33269263634166535 1108 1 0.10824361176310404 1108 2 0.22336531030051177 1108 3 0.22961546153151602 1108 4 0.20523579767105815 1108 5 0.016488386889534468 1108 6 0.13371031806451583 1108 7 0.18359322686089996 1108 8 0.3378513091675117 1108 9 0.3126587569663428 1108 10-11 0.27835502451918614 1108 12-13 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68-69 -1.013528221999529 2316 70-71 -1.2105030438251134 2316 72-73 -2.084551387872793 2316 74-75 -2.810643139104169 2316 76-77 -2.2062853505850697 2316 78-79 -1.9193870152304768 2316 80-81 -1.5311050302350324 2316 82-83 -1.359819512124723 2316 84-85 -1.136034957734065 2316 86-87 -0.9920842472490676 2316 88-89 -1.1004530266724473 2316 90-91 -1.0791613383807395 2316 92-93 -1.0376498449967677 2316 94-95 -1.7702156571766992 2316 96-97 -1.362210981387502 2316 98-99 -1.2544763801706722 2316 100-101 -1.7312792005010103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 9.0 5 31.0 6 61.0 7 167.0 8 411.0 9 827.0 10 1493.0 11 2380.0 12 3311.0 13 4435.0 14 5469.0 15 7276.0 16 8854.0 17 10314.0 18 8934.0 19 6633.0 20 6276.0 21 6492.0 22 7106.0 23 8213.0 24 9949.0 25 12776.0 26 16745.0 27 20959.0 28 26698.0 29 33057.0 30 40227.0 31 49114.0 32 59255.0 33 71593.0 34 89169.0 35 111032.0 36 144826.0 37 200177.0 38 193054.0 39 37914.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.44141819373628 22.697737710494984 15.711648322470637 22.149195773298096 2 15.110233411054041 22.098127235161844 40.52200104549566 22.26963830828846 3 17.967871665882583 26.706128047035044 31.926463767466593 23.399536519615776 4 12.298721224220442 18.662513235736572 39.55153916863783 29.487226371405157 5 11.755432573035852 39.12522920936253 36.54995312098106 12.569385096620561 6 28.663715140070295 40.45079369293724 17.62162601679981 13.263865150192657 7 24.99448249082766 34.123014733823744 23.30745220509536 17.575050570253243 8 24.321196105356172 37.92589888362947 20.35337213595576 17.399532875058597 9 24.652562862268038 16.46408143013838 21.885262359635398 36.99809334795819 10-11 23.51778131414623 28.012808918286105 29.117264908475725 19.352144859091943 12-13 24.055800685996477 25.459986027841435 29.750533912353745 20.733679373808343 14-15 21.283356039473837 27.312458618681205 27.33328410442944 24.070901237415516 16-17 20.15732783297131 30.36501019702071 28.51436294756126 20.963299022446723 18-19 19.985496833032705 28.907724015020904 31.179361362833063 19.927417789113335 20-21 20.63291223260823 28.065245997939027 31.39807044822087 19.903771321231872 22-23 20.49936444826476 27.828951953616336 31.240562139701904 20.431121458416996 24-25 20.35829274333361 28.156302025275394 31.345242683324987 20.140162548066012 26-27 20.31409980899784 28.311423123706668 31.187283338671218 20.18719372862427 28-29 20.103497156815116 28.36290049247024 31.289760925218452 20.243841425496193 30-31 20.550082663828924 28.03218599672664 31.22108062471866 20.196650714725777 32-33 20.462167125727284 28.292435582827174 30.94053346190903 20.304863829536504 34-35 20.584275406304624 28.19215342799495 31.21733931513539 20.006231850565037 36-37 20.555870613804917 28.1135487446738 31.212961353484708 20.117619288036575 38-39 20.467347411198848 28.328627528419435 31.272873904064635 19.931151156317075 40-41 20.491698883905514 28.20197315117162 31.17082680103665 20.135501163886214 42-43 20.455875018981654 28.312412715509193 30.80010886963939 20.431603395869757 44-45 20.387176967032165 28.60119345198706 30.687348852994145 20.324280727986626 46-47 20.5501418731908 28.519560825822705 30.395465036628966 20.53483226435753 48-49 20.66611928427609 28.775081848050355 30.043327293871734 20.51547157380182 50-51 20.72371442619939 28.845291400607408 29.75133720623338 20.679656966959815 52-53 20.712433931216196 29.016271839143954 29.67382392788524 20.59747030175461 54-55 20.629000389199025 29.02391540854694 29.719660525509962 20.627423676744073 56-57 20.582206145251163 29.094500422967794 29.64790575970534 20.675387672075704 58-59 20.560116743545517 28.95029146468421 29.87358453222709 20.616007259543192 60-61 20.648119533721456 29.174641784299656 29.773730959406652 20.403507722572243 62-63 20.614419202315457 29.006740937576676 29.827588357847723 20.551251502260147 64-65 20.428884048098492 28.918815287249338 29.72960609107839 20.922694573573775 66-67 20.410994057771916 28.97933176032944 29.662062032585553 20.947612149313088 68-69 20.426804035386365 28.98480262881541 29.77293153592807 20.815461799870157 70-71 20.52231032141118 28.73925195208295 29.81961867795216 20.91881904855371 72-73 20.603919225346523 29.038535295591565 29.567425859540663 20.790119619521246 74-75 20.520229771268607 29.28402374247649 29.38081551330419 20.814930972950712 76-77 20.44050453362733 29.21222431051907 29.50451658283686 20.842754573016737 78-79 20.331790283515698 29.144972282355713 29.586853182363924 20.93638425176466 80-81 20.366246450955565 29.239894661208343 29.430642835452115 20.963216052383977 82-83 20.627602148592743 29.12269944758532 29.527551868734726 20.72214653508721 84-85 20.60366528549169 29.148959472443153 29.32647392167958 20.92090132038558 86-87 20.34571136042693 29.26822893763472 29.458728201690263 20.92733150024808 88-89 20.519989977216422 29.090133697959104 29.359786401870476 21.030089922953998 90-91 20.454869811978295 29.02022934333461 29.560895467896913 20.96400537679019 92-93 20.549184861421672 29.063131073187996 29.362083681004915 21.02560038438542 94-95 20.174274802256512 29.04615992227165 29.43538249767885 21.344182777792987 96-97 20.253241225501014 28.90017373931138 29.468394213999705 21.378190821187896 98-99 20.081078345311447 29.033091779824005 29.433339362491225 21.452490512373327 100-101 20.12094546045896 29.01873795896964 29.343607239635794 21.516709340935602 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 267.0 1 191.0 2 163.5 3 224.5 4 175.5 5 154.5 6 332.5 7 768.5 8 1250.0 9 1615.0 10 1446.0 11 1008.5 12 972.5 13 1109.0 14 1320.5 15 1500.5 16 1649.0 17 1793.5 18 1986.5 19 2293.5 20 2638.0 21 2997.0 22 3604.0 23 4595.5 24 5794.0 25 7374.0 26 9597.5 27 11800.0 28 14008.0 29 17084.5 30 20459.0 31 23932.0 32 28201.5 33 32287.0 34 35299.0 35 38268.0 36 42878.5 37 47783.0 38 49454.5 39 49126.0 40 48150.0 41 46748.0 42 45876.0 43 46400.0 44 47480.5 45 48779.5 46 48553.0 47 46864.0 48 46146.5 49 44570.5 50 41839.0 51 38711.0 52 35038.0 53 31216.5 54 27694.0 55 23363.5 56 18634.0 57 14615.5 58 11241.5 59 8469.0 60 6187.5 61 4299.0 62 2914.0 63 1815.5 64 1039.5 65 617.5 66 331.5 67 172.5 68 91.5 69 46.5 70 23.5 71 17.0 72 10.5 73 7.5 74 8.5 75 7.5 76 4.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004895233701775725 2 0.006969485270324761 3 0.002074251568549036 4 0.17191397000134412 5 0.0019912815058070747 6 1.659401254839229E-4 7 0.0 8 7.46730564677653E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.659401254839229E-4 24-25 8.711856587905951E-4 26-27 0.0026550420077427663 28-29 2.903952195968651E-4 30-31 0.005932359486050243 32-33 0.021323306124684093 34-35 0.012818874693633044 36-37 7.882155960486337E-4 38-39 4.148503137098072E-4 40-41 0.0020327665371780556 42-43 0.011864718972100487 44-45 0.007840670929115357 46-47 0.010661653062342047 48-49 0.0103297728113742 50-51 0.18913025802030112 52-53 0.11574323752503622 54-55 0.01783856348952171 56-57 0.006596119987985935 58-59 0.01821192877186054 60-61 0.12528479474036178 62-63 0.24053021188894624 64-65 0.24650405640636747 66-67 0.25683382921774167 68-69 0.25218750570419185 70-71 0.2393271459791878 72-73 0.16432220926045463 74-75 0.26459153008411507 76-77 0.20904307307837186 78-79 0.0053930540782274935 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006139784642905146 92-93 0.017714108395408767 94-95 0.0025720719450008046 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1205254.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.50243716071395 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33953633893145 46.01890901046021 2 13.815339263808532 16.440927127974227 3 3.986905580472011 7.116917963032067 4 1.6538426909405741 3.9363068316559024 5 0.837447290013652 2.4915077374723755 6 0.5170812218127281 1.846055574473823 7 0.3320144374956241 1.3828967742473215 8 0.23664596633214652 1.126480939281198 9 0.17477069087820557 0.9359353846363491 >10 0.9966894769720159 11.105349588806728 >50 0.07799083697728446 3.149792356907467 >100 0.03019833173194708 3.00302330032838 >500 9.786468579296038E-4 0.4132799227015778 >1k 5.592267759597737E-4 1.0326174880222796 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4463 0.37029539001737394 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4086 0.3390156763636545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2746 0.22783579228942613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.297006274196145E-5 0.0 20-21 0.0 0.0 0.0 8.297006274196145E-5 0.0 22-23 0.0 0.0 0.0 8.297006274196145E-5 0.0 24-25 0.0 0.0 0.0 2.4891018822588434E-4 0.0 26-27 0.0 0.0 0.0 3.7336528233882654E-4 0.0 28-29 0.0 0.0 0.0 0.0013275210038713832 0.0 30-31 0.0 0.0 0.0 0.002820982133226689 0.0 32-33 0.0 0.0 0.0 0.007508790678147511 0.0 34-35 0.0 0.0 0.0 0.01493461129355306 0.0 36-37 0.0 0.0 0.0 0.02738012070484728 0.0 38-39 0.0 0.0 0.0 0.04472086381791722 0.0 40-41 0.0 0.0 0.0 0.06475813397010091 0.0 42-43 0.0 0.0 0.0 0.08603995506341403 0.0 44-45 0.0 0.0 0.0 0.11200958470164796 0.0 46-47 0.0 0.0 0.0 0.14561246011214235 0.0 48-49 0.0 0.0 0.0 0.18075028168336302 0.0 50-51 0.0 0.0 0.0 0.22223531305434374 0.0 52-53 0.0 0.0 0.0 0.26455004505274404 0.0 54-55 0.0 0.0 0.0 0.3116355556588072 0.0 56-57 0.0 0.0 0.0 0.3697145995781802 0.0 58-59 0.0 0.0 0.0 0.43737668574424976 0.0 60-61 0.0 0.0 0.0 0.5092702451101594 0.0 62-63 0.0 0.0 0.0 0.5843166668602635 0.0 64-65 4.1485031370980724E-5 0.0 0.0 0.6639264420611755 0.0 66-67 8.297006274196145E-5 0.0 0.0 0.7607525052810444 0.0 68-69 8.297006274196145E-5 0.0 0.0 0.8657926047123676 0.0 70-71 8.297006274196145E-5 0.0 0.0 0.9824899979589364 0.0 72-73 8.297006274196145E-5 0.0 0.0 1.1057835111934913 0.0 74-75 8.297006274196145E-5 0.0 0.0 1.232893647314176 0.0 76-77 8.297006274196145E-5 0.0 0.0 1.379252837990996 0.0 78-79 8.297006274196145E-5 0.0 0.0 1.5608743053331497 0.0 80-81 8.297006274196145E-5 0.0 0.0 1.7554805874944202 0.0 82-83 8.297006274196145E-5 0.0 0.0 1.9533227021026274 0.0 84-85 8.297006274196145E-5 0.0 0.0 2.150169175957931 0.0 86-87 8.297006274196145E-5 0.0 0.0 2.369915387130016 0.0 88-89 1.659401254839229E-4 0.0 0.0 2.6212317071754168 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2315 0.0 61.581295 1 GTATCAA 4020 0.0 38.772728 1 GTGGTAT 915 0.0 31.160809 1 ATCAACG 5275 0.0 28.916325 3 TATCAAC 5320 0.0 28.671732 2 CAACGCA 5375 0.0 28.335964 5 TCAACGC 5440 0.0 28.039267 4 TGGTATC 1020 0.0 27.486052 2 AACGCAG 5595 0.0 27.391376 6 GTGTACG 70 0.008374309 27.15442 1 ACGCAGA 6235 0.0 24.427555 7 CGCAGAG 6295 0.0 24.194729 8 GAGTACT 4910 0.0 22.129185 12-13 GCAGAGT 6865 0.0 21.56381 9 CAGAGTA 6805 0.0 21.021833 10-11 CATTGCG 115 0.0037474078 20.629257 9 GTACATG 3785 0.0 20.46446 1 AGAGTAC 6605 0.0 20.22167 10-11 GTACTTT 5385 0.0 20.13316 14-15 ACATGGG 3665 0.0 19.83709 3 >>END_MODULE