FastQCFastQC Report
Sat 18 Jun 2016
SRR1931017_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931017_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728544
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN740424.283489457022789No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20720.1198696706592369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.056.524831
GTATCAA31100.045.1758271
ATCAACG37500.036.037733
TCAACGC38950.034.817714
CAACGCA39000.034.7686275
TATCAAC39450.034.443872
AACGCAG42100.031.5857016
TCTAACG1552.2633685E-829.6611612
GTGGTAT8600.028.5152071
ACGCAGA47900.026.901577
CGCAGAG49300.026.012688
ACGGGCG1558.680872E-725.9206965
TGGTATC9100.024.6995262
GTACATG35550.024.2873941
ACATGGG34550.023.55613
GCAGAGT54000.023.4370049
CTAACGC1552.534464E-522.6883433
TACATGG36650.022.4403292
GTCTACG2053.387686E-722.4296461
GGCGTCG1803.6388155E-722.2643248
Produced by FastQC (version 0.11.5)