##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931017_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1728544 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.187513884517838 31.0 10.0 31.0 2.0 34.0 2 21.757366315234094 31.0 2.0 31.0 2.0 34.0 3 22.570275329988707 31.0 10.0 31.0 2.0 34.0 4 24.128634851065407 35.0 2.0 37.0 2.0 37.0 5 23.449809782105632 33.0 2.0 37.0 2.0 37.0 6 24.56823951256086 35.0 2.0 37.0 2.0 37.0 7 24.928528287391007 35.0 10.0 37.0 2.0 37.0 8 25.474558356628467 35.0 11.0 37.0 2.0 37.0 9 27.139975031008756 35.0 17.0 39.0 2.0 39.0 10-11 27.681079567543552 35.0 17.5 39.0 2.0 39.0 12-13 27.658438257863267 35.0 17.0 39.0 2.0 39.0 14-15 27.752113339319102 36.0 10.5 40.0 2.0 41.0 16-17 26.502815664513022 36.0 2.0 39.0 2.0 41.0 18-19 25.311321551548588 34.0 2.0 39.0 2.0 41.0 20-21 24.949031959845975 34.0 2.0 39.0 2.0 40.5 22-23 24.598367469963158 34.0 2.0 39.0 2.0 40.0 24-25 24.48400734953811 33.0 2.0 39.0 2.0 40.0 26-27 24.229226736490364 33.0 2.0 39.0 2.0 40.0 28-29 24.10563254392136 33.0 2.0 38.5 2.0 40.0 30-31 24.233588210655903 33.0 2.0 38.0 2.0 40.0 32-33 24.179479955384416 33.0 2.0 38.0 2.0 40.0 34-35 23.906604922987206 32.5 2.0 38.0 2.0 40.0 36-37 23.40182980589444 31.0 2.0 38.0 2.0 40.0 38-39 23.390473716607737 31.0 2.0 38.0 2.0 40.0 40-41 23.364647356387806 31.0 2.0 38.0 2.0 40.0 42-43 23.28708815048966 31.0 2.0 38.0 2.0 40.0 44-45 22.9285456430383 31.0 2.0 38.0 2.0 40.0 46-47 22.616522923338948 30.0 2.0 38.0 2.0 40.0 48-49 22.47239700001851 30.0 2.0 38.0 2.0 40.0 50-51 19.62387824666309 21.0 2.0 36.0 2.0 39.0 52-53 21.960420735601755 29.0 2.0 37.0 2.0 39.0 54-55 22.90083185617491 31.0 2.0 38.0 2.0 40.0 56-57 22.942340490030915 31.0 2.0 38.0 2.0 40.0 58-59 22.700241648462523 31.0 2.0 38.0 2.0 40.0 60-61 22.43634613871559 31.0 2.0 38.0 2.0 40.0 62-63 22.02109376446304 30.0 2.0 37.5 2.0 40.0 64-65 21.736398089953163 29.0 2.0 37.0 2.0 40.0 66-67 21.4379659991299 29.0 2.0 36.5 2.0 39.0 68-69 21.021039961956422 28.0 2.0 36.0 2.0 39.0 70-71 20.71951625182813 28.0 2.0 35.5 2.0 39.0 72-73 20.345144236999463 27.5 2.0 35.0 2.0 38.5 74-75 19.918933796304866 26.0 2.0 35.0 2.0 38.0 76-77 19.558695642112667 26.0 2.0 34.0 2.0 37.0 78-79 19.23702723216765 26.0 2.0 34.0 2.0 36.5 80-81 18.850400105522333 25.0 2.0 34.0 2.0 36.0 82-83 18.42944697965455 24.5 2.0 34.0 2.0 35.5 84-85 18.100986147879368 23.0 2.0 34.0 2.0 35.0 86-87 17.651215415980154 20.5 2.0 33.0 2.0 35.0 88-89 17.281353266101412 19.5 2.0 33.0 2.0 35.0 90-91 16.84391690347483 17.5 2.0 32.5 2.0 35.0 92-93 16.328442029823947 8.5 2.0 32.0 2.0 35.0 94-95 15.8025445114501 2.0 2.0 32.0 2.0 34.0 96-97 15.172715881111502 2.0 2.0 32.0 2.0 34.0 98-99 14.467285183368197 2.0 2.0 31.0 2.0 34.0 100-101 13.243275554455078 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 8.3251605432975 1101 2 10.151954801926237 1101 3 9.262202666258915 1101 4 11.582451084531666 1101 5 12.410297092362708 1101 6 11.494482134618064 1101 7 10.979188292572545 1101 8 10.193410741948185 1101 9 10.137573085626848 1101 10-11 9.371407114679865 1101 12-13 9.386475959995249 1101 14-15 10.322037680699186 1101 16-17 11.537746749096708 1101 18-19 12.648193301778846 1101 20-21 12.788190237939887 1101 22-23 13.09983389308832 1101 24-25 13.065441299701543 1101 26-27 13.27981122673275 1101 28-29 13.15291124468271 1101 30-31 12.69671252953086 1101 32-33 12.765846330001509 1101 34-35 12.704303515975447 1101 36-37 13.080565427187317 1101 38-39 12.93378521967179 1101 40-41 12.517287945820389 1101 42-43 12.271504070346989 1101 44-45 12.294782363375315 1101 46-47 12.282963536757006 1101 48-49 12.434656310273674 1101 50-51 14.34286764473806 1101 52-53 12.785422708472971 1101 54-55 12.411953956566226 1101 56-57 12.301125520006533 1101 58-59 12.37100901055025 1101 60-61 12.06286986920621 1101 62-63 12.10236271304657 1101 64-65 12.118718107541998 1101 66-67 12.028757585588501 1101 68-69 11.890657029293461 1101 70-71 11.726092484410014 1101 72-73 11.756448273341519 1101 74-75 11.572549792271499 1101 76-77 11.370528433414297 1101 78-79 11.125431717067517 1101 80-81 11.079478163715784 1101 82-83 10.917969542418163 1101 84-85 10.910327964014424 1101 86-87 10.711339217710584 1101 88-89 10.54410518971575 1101 90-91 10.323049946388405 1101 92-93 10.320792728698544 1101 94-95 10.2940552563388 1101 96-97 10.202818472806516 1101 98-99 9.917410200699743 1101 100-101 9.338732894549226 1102 1 8.005533581173282 1102 2 9.702160720001999 1102 3 9.039452126515023 1102 4 11.097888855164246 1102 5 11.974575295958733 1102 6 10.845371038651965 1102 7 10.619211975585273 1102 8 9.925350314972427 1102 9 9.752816621478743 1102 10-11 8.910801116768258 1102 12-13 8.895859714860247 1102 14-15 9.85486713136525 1102 16-17 10.943135182147774 1102 18-19 12.261694079448176 1102 20-21 12.47724694573337 1102 22-23 12.537227556847956 1102 24-25 12.412734852226475 1102 26-27 12.589755658060529 1102 28-29 12.346162072857954 1102 30-31 12.242530518397654 1102 32-33 12.260674950666825 1102 34-35 12.13164589493772 1102 36-37 12.358995387050328 1102 38-39 12.209993527955007 1102 40-41 12.028237383926925 1102 42-43 11.82229810845887 1102 44-45 11.56502945289613 1102 46-47 11.601962826023222 1102 48-49 11.329918308165816 1102 50-51 13.47547486939565 1102 52-53 12.221660212823785 1102 54-55 11.707367898144877 1102 56-57 11.724111916269823 1102 58-59 11.895799198042791 1102 60-61 11.794859339636517 1102 62-63 12.122611019577288 1102 64-65 11.76255772510881 1102 66-67 11.633599642043158 1102 68-69 11.470764272206207 1102 70-71 11.038401565981314 1102 72-73 11.044561068253447 1102 74-75 10.74114460829611 1102 76-77 10.632636769201934 1102 78-79 10.244027810952527 1102 80-81 9.997411839110033 1102 82-83 9.983852982077597 1102 84-85 9.862171794869521 1102 86-87 9.784151094753636 1102 88-89 9.466425273056593 1102 90-91 9.52630794275198 1102 92-93 9.491036593143116 1102 94-95 9.251047647592882 1102 96-97 9.27693255044688 1102 98-99 9.10798056456136 1102 100-101 8.524398099992668 1103 1 8.15815940174221 1103 2 9.889250435538106 1103 3 8.978730025858567 1103 4 11.226991699803197 1103 5 12.07687288895654 1103 6 10.99690276731717 1103 7 10.641093813659673 1103 8 9.961455347795187 1103 9 10.041109838108945 1103 10-11 9.091052757906116 1103 12-13 8.88655993367863 1103 14-15 9.893707393947306 1103 16-17 11.167150499434424 1103 18-19 12.153105458003978 1103 20-21 12.516907777243219 1103 22-23 12.813212239561306 1103 24-25 12.670393495552513 1103 26-27 12.620116708388753 1103 28-29 12.379531875921405 1103 30-31 12.232957214240098 1103 32-33 12.232775607121965 1103 34-35 12.332138236294398 1103 36-37 12.701993198866518 1103 38-39 12.433188276313864 1103 40-41 11.908434320469592 1103 42-43 11.747899859005923 1103 44-45 11.922523982436612 1103 46-47 11.914599587511187 1103 48-49 12.02904303464284 1103 50-51 13.90818821731688 1103 52-53 12.361156930548077 1103 54-55 11.887346016306804 1103 56-57 11.580785876882512 1103 58-59 11.64935499672988 1103 60-61 11.670406385588379 1103 62-63 11.90297206990552 1103 64-65 11.539089453774022 1103 66-67 11.522002267863726 1103 68-69 11.178094687961124 1103 70-71 11.204703535346741 1103 72-73 11.1162240879471 1103 74-75 11.035181807420837 1103 76-77 10.779792130252265 1103 78-79 10.524662384256683 1103 80-81 10.255070482436071 1103 82-83 10.131008343127924 1103 84-85 9.852872013687904 1103 86-87 9.8011095192613 1103 88-89 9.661720677870598 1103 90-91 9.373408599207124 1103 92-93 9.196999394018386 1103 94-95 9.039067341247149 1103 96-97 8.827260778017997 1103 98-99 8.513068091913661 1103 100-101 7.536159587957654 1104 1 8.0190909845285 1104 2 9.794102076675959 1104 3 8.960469777864404 1104 4 11.183659241743388 1104 5 11.938982298146914 1104 6 10.902246020417099 1104 7 10.519031377481703 1104 8 9.831862058225525 1104 9 9.686412536868971 1104 10-11 8.958614975265707 1104 12-13 8.79265942310241 1104 14-15 9.486312207951688 1104 16-17 10.793148603008458 1104 18-19 12.025774021096609 1104 20-21 12.224578966156429 1104 22-23 12.464887804842125 1104 24-25 12.363195100220647 1104 26-27 12.474291908972273 1104 28-29 12.451395041493985 1104 30-31 12.089755317814134 1104 32-33 12.075136949208037 1104 34-35 11.963165807410377 1104 36-37 12.080497648173598 1104 38-39 11.761544804395555 1104 40-41 11.527940374444793 1104 42-43 11.220154125964338 1104 44-45 11.432771684843612 1104 46-47 11.461586896045102 1104 48-49 11.126754048501333 1104 50-51 13.083067867207472 1104 52-53 11.988161306915693 1104 54-55 11.435176796759027 1104 56-57 11.291896598983175 1104 58-59 11.541024435096038 1104 60-61 11.220361600759787 1104 62-63 11.313492857651685 1104 64-65 11.050145471279489 1104 66-67 10.922466892225508 1104 68-69 10.687191697443257 1104 70-71 10.625325854821579 1104 72-73 10.58292124330815 1104 74-75 10.259193915371238 1104 76-77 10.158996360740957 1104 78-79 9.99628703779425 1104 80-81 9.89117011773877 1104 82-83 9.608600465666218 1104 84-85 9.37934539078491 1104 86-87 9.150542925534094 1104 88-89 8.969342267950829 1104 90-91 8.81463719147554 1104 92-93 8.454718868854274 1104 94-95 8.370924963420746 1104 96-97 8.050702937026017 1104 98-99 7.832031038667855 1104 100-101 7.252199412902952 1105 1 8.002891332112789 1105 2 9.581624078761465 1105 3 8.851487365629438 1105 4 11.00209705891405 1105 5 11.453051119836172 1105 6 10.826442336023636 1105 7 10.27994594126342 1105 8 9.792963483321117 1105 9 9.62279766026139 1105 10-11 8.748648482391179 1105 12-13 8.49028931535128 1105 14-15 9.567892187096621 1105 16-17 10.80479587447179 1105 18-19 11.58871347191343 1105 20-21 12.063415810091072 1105 22-23 12.207092740539029 1105 24-25 12.331296594833088 1105 26-27 12.54274849569455 1105 28-29 12.228536925400828 1105 30-31 11.75433564454337 1105 32-33 11.637424470932054 1105 34-35 11.826424201570958 1105 36-37 12.122473595340786 1105 38-39 11.721394787016344 1105 40-41 11.342659317788549 1105 42-43 11.178128682794377 1105 44-45 11.12718419787802 1105 46-47 11.039753319402767 1105 48-49 11.100274521215965 1105 50-51 13.066342111211636 1105 52-53 11.655740034757194 1105 54-55 11.32022650131238 1105 56-57 11.296108069264715 1105 58-59 11.39278474097021 1105 60-61 11.266888121441056 1105 62-63 11.216312461405597 1105 64-65 11.040644741352477 1105 66-67 10.68705013866277 1105 68-69 10.6241002827752 1105 70-71 10.548618034175075 1105 72-73 10.312590482098557 1105 74-75 10.183480602892963 1105 76-77 9.89531266244412 1105 78-79 9.621873134422682 1105 80-81 9.381058473665082 1105 82-83 9.12560234262138 1105 84-85 8.950412474552724 1105 86-87 8.95190253623969 1105 88-89 8.746662254047457 1105 90-91 8.575251442431398 1105 92-93 8.453130130585246 1105 94-95 8.049805185874687 1105 96-97 7.7994932046659216 1105 98-99 7.390012130082523 1105 100-101 6.901602541161553 1106 1 7.856359568128074 1106 2 9.37048716719779 1106 3 8.873401833242465 1106 4 10.887343160806221 1106 5 11.529751201235303 1106 6 10.660509705188137 1106 7 9.870509971730904 1106 8 9.38313106781019 1106 9 8.966881120468543 1106 10-11 8.27122924576624 1106 12-13 8.14148626016311 1106 14-15 9.032030205821759 1106 16-17 10.274505791506865 1106 18-19 11.617901390638671 1106 20-21 12.007619647732568 1106 22-23 12.181293768633392 1106 24-25 11.846204684885176 1106 26-27 11.881626520260877 1106 28-29 11.784451910503567 1106 30-31 11.52348609523693 1106 32-33 11.6128242761516 1106 34-35 11.262069747772458 1106 36-37 11.846230981506935 1106 38-39 11.375526487036666 1106 40-41 11.248850704583237 1106 42-43 11.039131335761997 1106 44-45 10.66939958686704 1106 46-47 10.584444830017414 1106 48-49 10.652141481941158 1106 50-51 12.732599283607893 1106 52-53 11.559213490089277 1106 54-55 10.785247084298963 1106 56-57 10.681007142114165 1106 58-59 10.63352794947303 1106 60-61 10.455151377117616 1106 62-63 10.669652602060633 1106 64-65 10.456135673622402 1106 66-67 10.14114846837034 1106 68-69 10.056789141532715 1106 70-71 9.823484747260341 1106 72-73 9.76387843180655 1106 74-75 9.79735110386964 1106 76-77 9.507743964574821 1106 78-79 9.002579263566343 1106 80-81 8.689766070880413 1106 82-83 8.772509730841936 1106 84-85 8.441081280028786 1106 86-87 8.218936109772642 1106 88-89 7.9994670815823525 1106 90-91 7.7458303757140925 1106 92-93 7.613062212837233 1106 94-95 7.315098339352586 1106 96-97 6.870167473767232 1106 98-99 6.860062135579463 1106 100-101 6.250548294692086 1107 1 7.578078428313351 1107 2 9.05351448898033 1107 3 8.633666252050407 1107 4 10.654174658185195 1107 5 11.316890555027651 1107 6 10.278676601456855 1107 7 10.05227663742054 1107 8 8.782531171375375 1107 9 6.493780768565323 1107 10-11 7.967704523745642 1107 12-13 7.896611644725002 1107 14-15 8.575917388739601 1107 16-17 9.890062868164062 1107 18-19 11.363544411454583 1107 20-21 11.691909637140412 1107 22-23 11.333384153106199 1107 24-25 11.547666408009814 1107 26-27 11.563887735559792 1107 28-29 11.601318121184573 1107 30-31 11.110618638555277 1107 32-33 11.462870426452149 1107 34-35 11.627850966611668 1107 36-37 11.973277212843499 1107 38-39 11.467918756545231 1107 40-41 11.14124650864159 1107 42-43 10.892781864766288 1107 44-45 10.49339078899904 1107 46-47 10.838093749795778 1107 48-49 10.984568907288889 1107 50-51 12.95291136423931 1107 52-53 11.440204480148289 1107 54-55 10.882019034571588 1107 56-57 10.557233673996667 1107 58-59 10.896326611746513 1107 60-61 10.7726058173455 1107 62-63 10.878235437252332 1107 64-65 10.769179254333991 1107 66-67 10.920749317122073 1107 68-69 10.550514597245378 1107 70-71 10.429223534260327 1107 72-73 10.230442883890987 1107 74-75 10.174376070435098 1107 76-77 9.771722390602442 1107 78-79 9.737972664705563 1107 80-81 9.580720324610514 1107 82-83 9.1780238871449 1107 84-85 9.062169639750554 1107 86-87 9.02430928639036 1107 88-89 9.021485315261497 1107 90-91 8.496022764420314 1107 92-93 8.17767019103452 1107 94-95 7.976975116325944 1107 96-97 7.632192989193676 1107 98-99 7.304933934872208 1107 100-101 7.021351706480933 1108 1 7.18973109873264 1108 2 8.693431412864395 1108 3 8.310504610127072 1108 4 10.128764737817615 1108 5 11.561350606498092 1108 6 10.013927961887479 1108 7 9.320148864990621 1108 8 6.402529010402542 1108 9 7.441580274199453 1108 10-11 6.27782853700732 1108 12-13 5.266218024101693 1108 14-15 7.66448694028573 1108 16-17 9.260239680810027 1108 18-19 9.230787154572695 1108 20-21 9.634758694921587 1108 22-23 10.10695190191564 1108 24-25 10.01190245211215 1108 26-27 8.689170424318451 1108 28-29 10.291979338424742 1108 30-31 10.394836545508635 1108 32-33 10.843260146923953 1108 34-35 10.347731974433927 1108 36-37 10.274585728116492 1108 38-39 10.062240164852376 1108 40-41 10.079227455101464 1108 42-43 9.427367830461126 1108 44-45 8.936659907541689 1108 46-47 9.962819872490503 1108 48-49 10.431849837223673 1108 50-51 12.407436175558647 1108 52-53 11.13474374802918 1108 54-55 10.658227474846111 1108 56-57 10.329861052944633 1108 58-59 10.498991744160993 1108 60-61 10.277633834878273 1108 62-63 10.381912557865821 1108 64-65 10.287360460572845 1108 66-67 9.775645336194103 1108 68-69 10.144502903724483 1108 70-71 10.19188602612779 1108 72-73 9.929573063436628 1108 74-75 9.737849447134263 1108 76-77 9.109543113060731 1108 78-79 9.099567266202527 1108 80-81 9.003885606675244 1108 82-83 8.860340080227243 1108 84-85 8.526012057451577 1108 86-87 8.424526365933804 1108 88-89 8.150013074517567 1108 90-91 7.954364957357054 1108 92-93 7.6343081622161595 1108 94-95 7.72253038811739 1108 96-97 7.399617854941795 1108 98-99 7.268059446947655 1108 100-101 6.725558727678111 1109 1 6.724753560775358 1109 2 8.56936752339286 1109 3 8.120450258668136 1109 4 9.709168247843134 1109 5 10.828489294917635 1109 6 9.625725317151943 1109 7 8.142227358823646 1109 8 8.616430698340345 1109 9 7.881561442502267 1109 10-11 6.18250747616273 1109 12-13 6.718987804874917 1109 14-15 8.09949972140701 1109 16-17 7.090276410686972 1109 18-19 6.66351022489998 1109 20-21 8.955139281908131 1109 22-23 10.1620573778597 1109 24-25 9.975131949879778 1109 26-27 10.435439235847046 1109 28-29 10.625744951791791 1109 30-31 8.080733286956583 1109 32-33 7.19915819068633 1109 34-35 9.006975064676247 1109 36-37 7.346410385884397 1109 38-39 7.805323204108511 1109 40-41 8.609339154495025 1109 42-43 7.455951327256052 1109 44-45 7.8731049464151965 1109 46-47 7.192415313698493 1109 48-49 8.223180456825698 1109 50-51 10.31908509002771 1109 52-53 10.421797474838186 1109 54-55 10.297601626324866 1109 56-57 9.314009408455721 1109 58-59 9.326408862796075 1109 60-61 9.482758730289746 1109 62-63 9.824121490590333 1109 64-65 9.908749131128786 1109 66-67 9.683387595490665 1109 68-69 9.34053435980422 1109 70-71 8.87991487527368 1109 72-73 8.128536743846364 1109 74-75 7.039117417344688 1109 76-77 7.045861089840351 1109 78-79 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-20.517442348804835 2314 2 -19.05549792332404 2314 3 -19.877396888802263 2314 4 -21.31020742492328 2314 5 -20.573017701853086 2314 6 -21.758020646249566 2314 7 -22.122035289184964 2314 8 -22.74040460844114 2314 9 -24.406920796464362 2314 10-11 -25.01151835806763 2314 12-13 -25.00840724356426 2314 14-15 -25.060887792048316 2314 16-17 -23.71058473032487 2314 18-19 -22.469159312236727 2314 20-21 -22.09195436717691 2314 22-23 -21.73164552849121 2314 24-25 -21.60613823311269 2314 26-27 -21.367574757694396 2314 28-29 -21.221671625172686 2314 30-31 -21.394778015519197 2314 32-33 -21.332596384125296 2314 34-35 -21.089634192589628 2314 36-37 -20.570435685159737 2314 38-39 -20.552588528937775 2314 40-41 -20.515526292221875 2314 42-43 -20.43344587403566 2314 44-45 -20.104828315156386 2314 46-47 -19.796013103954895 2314 48-49 -19.647379284832 2314 50-51 -16.97693213279253 2314 52-53 -19.19983869308431 2314 54-55 -20.08748986990764 2314 56-57 -20.124903401016827 2314 58-59 -19.885108898237295 2314 60-61 -19.634571732573548 2314 62-63 -19.230507142348312 2314 64-65 -18.966654528720515 2314 66-67 -18.682866441107826 2314 68-69 -18.278141635890076 2314 70-71 -17.99387414517842 2314 72-73 -17.63787875669185 2314 74-75 -17.187739417962096 2314 76-77 -16.858336972592376 2314 78-79 -16.56477962887242 2314 80-81 -16.18776321559456 2314 82-83 -15.784199534333784 2314 84-85 -15.473987942548423 2314 86-87 -15.035323741132576 2314 88-89 -14.672524398715836 2314 90-91 -14.258429475191125 2314 92-93 -13.755681131145726 2314 94-95 -13.243208369912589 2314 96-97 -12.631163729640647 2314 98-99 -11.953835627998812 2314 100-101 -10.781400587097048 2315 1 -20.517442348804835 2315 2 -19.05549792332404 2315 3 -19.877396888802263 2315 4 -21.31020742492328 2315 5 -20.573017701853086 2315 6 -21.758020646249566 2315 7 -22.122035289184964 2315 8 -22.74040460844114 2315 9 -24.406920796464362 2315 10-11 -25.01151835806763 2315 12-13 -25.00840724356426 2315 14-15 -25.060887792048316 2315 16-17 -23.71058473032487 2315 18-19 -22.469159312236727 2315 20-21 -22.09195436717691 2315 22-23 -21.73164552849121 2315 24-25 -21.60613823311269 2315 26-27 -21.367574757694396 2315 28-29 -21.221671625172686 2315 30-31 -21.394778015519197 2315 32-33 -21.332596384125296 2315 34-35 -21.089634192589628 2315 36-37 -20.570435685159737 2315 38-39 -20.552588528937775 2315 40-41 -20.515526292221875 2315 42-43 -20.43344587403566 2315 44-45 -20.104828315156386 2315 46-47 -19.796013103954895 2315 48-49 -19.647379284832 2315 50-51 -16.97693213279253 2315 52-53 -19.19983869308431 2315 54-55 -20.08748986990764 2315 56-57 -20.124903401016827 2315 58-59 -19.885108898237295 2315 60-61 -19.634571732573548 2315 62-63 -19.230507142348312 2315 64-65 -18.966654528720515 2315 66-67 -18.682866441107826 2315 68-69 -18.278141635890076 2315 70-71 -17.99387414517842 2315 72-73 -17.63787875669185 2315 74-75 -17.187739417962096 2315 76-77 -16.858336972592376 2315 78-79 -16.56477962887242 2315 80-81 -16.18776321559456 2315 82-83 -15.784199534333784 2315 84-85 -15.473987942548423 2315 86-87 -15.035323741132576 2315 88-89 -14.672524398715836 2315 90-91 -14.258429475191125 2315 92-93 -13.755681131145726 2315 94-95 -13.243208369912589 2315 96-97 -12.631163729640647 2315 98-99 -11.953835627998812 2315 100-101 -10.781400587097048 2316 1 -20.517442348804835 2316 2 -19.05549792332404 2316 3 -19.877396888802263 2316 4 -21.31020742492328 2316 5 -20.573017701853086 2316 6 -21.758020646249566 2316 7 -22.122035289184964 2316 8 -22.74040460844114 2316 9 -24.406920796464362 2316 10-11 -25.01151835806763 2316 12-13 -25.00840724356426 2316 14-15 -25.060887792048316 2316 16-17 -23.71058473032487 2316 18-19 -22.469159312236727 2316 20-21 -22.09195436717691 2316 22-23 -21.73164552849121 2316 24-25 -21.60613823311269 2316 26-27 -21.367574757694396 2316 28-29 -21.221671625172686 2316 30-31 -21.394778015519197 2316 32-33 -21.332596384125296 2316 34-35 -21.089634192589628 2316 36-37 -20.570435685159737 2316 38-39 -20.552588528937775 2316 40-41 -20.515526292221875 2316 42-43 -20.43344587403566 2316 44-45 -20.104828315156386 2316 46-47 -19.796013103954895 2316 48-49 -19.647379284832 2316 50-51 -16.97693213279253 2316 52-53 -19.19983869308431 2316 54-55 -20.08748986990764 2316 56-57 -20.124903401016827 2316 58-59 -19.885108898237295 2316 60-61 -19.634571732573548 2316 62-63 -19.230507142348312 2316 64-65 -18.966654528720515 2316 66-67 -18.682866441107826 2316 68-69 -18.278141635890076 2316 70-71 -17.99387414517842 2316 72-73 -17.63787875669185 2316 74-75 -17.187739417962096 2316 76-77 -16.858336972592376 2316 78-79 -16.56477962887242 2316 80-81 -16.18776321559456 2316 82-83 -15.784199534333784 2316 84-85 -15.473987942548423 2316 86-87 -15.035323741132576 2316 88-89 -14.672524398715836 2316 90-91 -14.258429475191125 2316 92-93 -13.755681131145726 2316 94-95 -13.243208369912589 2316 96-97 -12.631163729640647 2316 98-99 -11.953835627998812 2316 100-101 -10.781400587097048 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 373607.0 3 14537.0 4 66046.0 5 31987.0 6 12009.0 7 12665.0 8 13337.0 9 10669.0 10 10295.0 11 11543.0 12 12647.0 13 14057.0 14 14764.0 15 16260.0 16 17788.0 17 18627.0 18 17173.0 19 16741.0 20 17787.0 21 19052.0 22 20985.0 23 23039.0 24 25649.0 25 28281.0 26 31788.0 27 36121.0 28 40432.0 29 46395.0 30 52971.0 31 62064.0 32 72953.0 33 86736.0 34 102254.0 35 120491.0 36 127928.0 37 97119.0 38 30742.0 39 1005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.4857649479625 21.079360038345357 14.242256494855063 23.192618518837083 2 14.710144519391152 20.7861384975487 42.22965878103554 22.27405820202461 3 18.49360705657488 26.537467278458095 30.161248196976263 24.807677467990764 4 11.88528154564225 17.126031174403362 40.0648903033368 30.923796976617584 5 12.242909902686092 38.45546741438175 36.18475353811447 13.116869144817686 6 29.37844969109974 39.17652510452916 18.176108969463613 13.268916234907483 7 25.96246757488278 33.0840181242797 23.32708919112706 17.626425109710457 8 24.328311121953103 37.12525673226947 20.79926630549477 17.747165840282662 9 25.293672228465287 16.592195567996754 21.506179329362265 36.607952874175695 10-11 23.70290445781157 27.495943632190055 29.337716258981473 19.4634356510169 12-13 24.26888383050491 25.018764881392684 29.62994327513244 21.082408012969957 14-15 21.68413800243105 26.652839530853136 27.17821119462527 24.484811272090546 16-17 20.80179926069981 29.85822657027063 27.863823280533246 21.476150888496313 18-19 20.61807968720313 28.57966047869126 30.172593054689123 20.629666779416485 20-21 21.441992967629194 28.011889335013016 30.09244274818345 20.453674949174335 22-23 21.101688529015274 27.730088941927182 29.896321471618688 21.271901057438853 24-25 20.983112943196232 27.988099793342673 30.146655492616752 20.882131770844335 26-27 20.854459068232504 28.182494456612478 29.942704375787855 21.020342099367166 28-29 20.754626581002444 28.255701727824185 30.120439441828236 20.86923224934514 30-31 21.113676166527952 28.040110612619205 30.053332485727058 20.792880735125784 32-33 20.951595127554036 28.262162230215132 29.844603140476977 20.941639501753855 34-35 20.992100771398707 28.221586688315988 30.00697491111241 20.779337629172897 36-37 21.076595674480757 28.10012435612515 29.904780758501666 20.91849921089243 38-39 20.936306561768987 28.36320210896724 29.91471851919158 20.785772810072192 40-41 21.08884976832621 28.201998482405028 29.938936312153295 20.770215437115464 42-43 20.970753837044683 28.27798877276191 29.649683686134892 21.101573704058517 44-45 20.897507985871904 28.580112729306457 29.581229771511353 20.94114951331029 46-47 21.080455680274472 28.401156363119384 29.30428522785781 21.214102728748333 48-49 21.050508776647163 28.595232751334628 29.205268432736815 21.148990039281394 50-51 21.313189796994042 28.704858726381193 28.810115159162418 21.171836317462343 52-53 20.92202235350155 28.89454634257597 28.994943126212103 21.188488177710376 54-55 20.83333953206056 28.860976433430874 29.082345380187775 21.223338654320788 56-57 20.883050015564812 28.921329903345384 29.04076834785731 21.154851733232498 58-59 20.81726814480275 28.848702058891863 29.155337476796127 21.178692319509263 60-61 20.88006522846133 29.010982481133613 29.06856134085406 21.04039094955099 62-63 20.736417961311165 28.83831412102665 29.112330630324294 21.312937287337892 64-65 20.643866160589454 28.773969338267303 29.214605957551587 21.367558543591656 66-67 20.718380615095644 28.864043358471474 28.847389278184714 21.57018674824817 68-69 20.780399785093266 28.795024255851647 28.978168043999563 21.446407915055524 70-71 20.750252641960458 28.654205207925624 29.066798460634658 21.52874368947926 72-73 20.79222157050581 28.914230238129825 28.853479644689934 21.440068546674436 74-75 20.68250211821077 29.076709515451714 28.724113825413827 21.516674540923688 76-77 20.677821132952918 28.973613990006932 28.824281304440397 21.524283572599757 78-79 20.611855182787792 28.99152911676094 28.779576064941025 21.617039635510242 80-81 20.7023442758289 29.037486124532414 28.560441316853357 21.69972828278533 82-83 20.86052304967165 28.868702920938276 28.78868888052915 21.482085148860918 84-85 20.862423188505115 28.966928225511694 28.583120602613306 21.587527983369885 86-87 20.69607473753271 29.058660002123887 28.730621353206704 21.514643907136698 88-89 20.824399583939968 28.956100792048122 28.630758191271482 21.588741432740427 90-91 20.789378034951007 28.910470762699543 28.768609519932543 21.53154168241691 92-93 20.82244911381348 28.879539169200818 28.516322059770943 21.781689657214763 94-95 20.597693812741966 28.846388837375127 28.700185593301963 21.855731756580944 96-97 20.63062686173475 28.807475797891385 28.682893347637506 21.879003992736354 98-99 20.48866926822812 28.7963706739302 28.43781702570139 22.27714303214029 100-101 20.65236290169616 28.87215414326852 28.37012374914502 22.1053592058903 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 99094.0 1 89894.5 2 59910.0 3 30582.0 4 18805.0 5 14749.0 6 13829.0 7 14201.5 8 14883.5 9 14667.0 10 13414.5 11 11460.0 12 9080.0 13 6924.5 14 5401.5 15 4460.5 16 4204.5 17 4455.0 18 5047.5 19 5845.0 20 6754.0 21 7764.5 22 8888.0 23 10187.5 24 11515.0 25 12880.0 26 14585.0 27 16571.0 28 18786.0 29 21247.0 30 24045.0 31 27253.0 32 31097.5 33 35418.5 34 39366.0 35 42663.5 36 46558.5 37 50295.5 38 52061.5 39 52775.0 40 52889.0 41 52371.0 42 51557.5 43 51668.5 44 51912.0 45 51928.0 46 50896.0 47 48959.0 48 47660.0 49 46229.0 50 44095.5 51 40974.0 52 37375.5 53 33661.0 54 29922.0 55 25704.5 56 21117.0 57 17054.0 58 13723.5 59 10807.0 60 8249.0 61 6032.0 62 4196.5 63 2815.5 64 1824.5 65 1108.0 66 662.5 67 408.5 68 245.0 69 143.0 70 89.0 71 55.0 72 39.0 73 32.5 74 21.0 75 14.5 76 9.5 77 6.5 78 3.5 79 1.0 80 2.0 81 3.5 82 2.5 83 0.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 21.305503360053315 2 28.100817798100596 3 22.0305644519318 4 28.159884851065403 5 31.16993261380677 6 24.09368809819131 7 22.94584343817687 8 19.130956458152063 9 11.297774311790732 10-11 14.802024131292002 12-13 14.947695864264954 14-15 18.970677055371457 16-17 22.0604161652813 18-19 25.856761528777977 20-21 27.877826656422975 22-23 27.452208332561973 24-25 26.067198752244664 26-27 27.441997426736027 28-29 24.53342234851991 30-31 26.286140242886496 32-33 25.328629181553957 34-35 23.484880917118687 36-37 26.35669094914564 38-39 26.461605837051298 40-41 23.643742942036766 42-43 24.423300766425385 44-45 23.246501101505082 46-47 26.346711451950313 48-49 21.89941361053002 50-51 27.55868522872429 52-53 21.11340527056297 54-55 22.225757631856638 56-57 24.26906691411963 58-59 23.55190264176093 60-61 23.086655589906883 62-63 22.92825638225003 64-65 21.52664323268601 66-67 22.01425014347335 68-69 22.955938639687506 70-71 21.514118240553902 72-73 21.34087995445878 74-75 18.81566219893737 76-77 20.93073708277024 78-79 20.08083103467427 80-81 21.09440083677361 82-83 21.8771405298332 84-85 18.9588462891312 86-87 20.462655275191143 88-89 19.296760741988635 90-91 20.41625784475258 92-93 20.393319464242737 94-95 19.515268341448063 96-97 18.41046568672825 98-99 17.825117555584352 100-101 19.47859007349538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1728544.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.12458442191195 #Duplication Level Percentage of deduplicated Percentage of total 1 85.6233239171101 60.8992333042721 2 9.396610192063736 13.36659989770471 3 2.3855481332207455 5.090133587813802 4 0.9445255617045486 2.6871595220843583 5 0.458786489559838 1.631549920416566 6 0.26884606793157906 1.1472938913059203 7 0.17821855966461464 0.8873004694692207 8 0.12195911881749406 0.6939433313885478 9 0.10158144431088968 0.650244421043066 >10 0.48827620184743936 6.104977057576982 >50 0.023395941530378103 1.134971750305691 >100 0.008588313593336524 0.960409631724207 >500 8.501466133944337E-5 0.05127544323991795 >1k 1.7002932267888674E-4 0.21786016744507591 >5k 0.0 0.0 >10k+ 8.501466133944337E-5 4.477047604209911 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 74042 4.283489457022789 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2072 0.1198696706592369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.7852157654071866E-5 0.0 22-23 0.0 0.0 0.0 1.1570431530814373E-4 0.0 24-25 0.0 0.0 0.0 1.1570431530814373E-4 0.0 26-27 0.0 0.0 0.0 1.4463039413517966E-4 0.0 28-29 0.0 0.0 0.0 4.3389118240553897E-4 0.0 30-31 0.0 0.0 0.0 9.545606012921859E-4 0.0 32-33 0.0 0.0 0.0 0.0032686469074550604 0.0 34-35 0.0 0.0 0.0 0.006537293814910121 0.0 36-37 0.0 0.0 0.0 0.012293583501490271 0.0 38-39 0.0 0.0 0.0 0.021289594016698447 0.0 40-41 0.0 0.0 0.0 0.0300252698224633 0.0 42-43 0.0 0.0 0.0 0.04038080604254216 0.0 44-45 0.0 0.0 0.0 0.053339689357054264 0.0 46-47 0.0 0.0 0.0 0.06959614565784845 0.0 48-49 0.0 0.0 0.0 0.08695179295407002 0.0 50-51 0.0 0.0 0.0 0.10737360460595738 0.0 52-53 0.0 0.0 0.0 0.12947312882981282 0.0 54-55 0.0 0.0 0.0 0.15813887294740545 0.0 56-57 0.0 0.0 0.0 0.1898129292630098 0.0 58-59 0.0 0.0 0.0 0.2265201232945184 0.0 60-61 0.0 0.0 0.0 0.26843401151489343 0.0 62-63 0.0 0.0 0.0 0.3125752078049503 0.0 64-65 0.0 0.0 0.0 0.35767096469629933 0.0 66-67 0.0 0.0 0.0 0.4121098450487809 0.0 68-69 0.0 0.0 0.0 0.4726810541125942 0.0 70-71 0.0 0.0 0.0 0.541756530351556 0.0 72-73 2.8926078827035933E-5 0.0 0.0 0.6147370272321677 0.0 74-75 5.7852157654071866E-5 0.0 0.0 0.6909572449414074 0.0 76-77 5.7852157654071866E-5 0.0 0.0 0.7764916600329526 0.0 78-79 5.7852157654071866E-5 0.0 0.0 0.882332182461077 0.0 80-81 5.7852157654071866E-5 0.0 0.0 1.0003795101542108 0.0 82-83 5.7852157654071866E-5 0.0 0.0 1.1233153451691134 0.0 84-85 5.7852157654071866E-5 0.0 0.0 1.247813188440676 0.0 86-87 5.7852157654071866E-5 0.0 0.0 1.38078637280856 0.0 88-89 5.7852157654071866E-5 0.0 0.0 1.5306523872114335 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1645 0.0 56.52483 1 GTATCAA 3110 0.0 45.175827 1 ATCAACG 3750 0.0 36.03773 3 TCAACGC 3895 0.0 34.81771 4 CAACGCA 3900 0.0 34.768627 5 TATCAAC 3945 0.0 34.44387 2 AACGCAG 4210 0.0 31.585701 6 TCTAACG 155 2.2633685E-8 29.661161 2 GTGGTAT 860 0.0 28.515207 1 ACGCAGA 4790 0.0 26.90157 7 CGCAGAG 4930 0.0 26.01268 8 ACGGGCG 155 8.680872E-7 25.920696 5 TGGTATC 910 0.0 24.699526 2 GTACATG 3555 0.0 24.287394 1 ACATGGG 3455 0.0 23.5561 3 GCAGAGT 5400 0.0 23.437004 9 CTAACGC 155 2.534464E-5 22.688343 3 TACATGG 3665 0.0 22.440329 2 GTCTACG 205 3.387686E-7 22.429646 1 GGCGTCG 180 3.6388155E-7 22.264324 8 >>END_MODULE