FastQCFastQC Report
Sat 18 Jun 2016
SRR1931017_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931017_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1728544
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51350.29707082955365904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.27931021715385895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.17280439491271266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.060.621111
GTATCAA54950.042.385631
ATCAACG69450.032.9823
CAACGCA69900.032.716585
TCAACGC70750.032.3078654
TATCAAC71350.032.1065032
AACGCAG73550.031.28476
TACCGGC1153.9023053E-628.8784037
GTGGTAT14200.027.1135861
CGCAGAG84250.027.08628
ACGCAGA84350.027.0540927
TGGTATC14300.024.2620092
GCAGAGT94300.023.74679
GAGTACT65600.020.7563512-13
AGAGTAC90600.020.65816510-11
CAGAGTA93550.020.5392310-11
GTACATG57700.020.5122741
GAGTACG2551.1836164E-820.5041981
ACATGGG55500.020.2935283
TACATGG58150.020.1059842
Produced by FastQC (version 0.11.5)