##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931017_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1728544 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.170452126182496 31.0 31.0 33.0 30.0 34.0 2 31.329368532128775 31.0 31.0 34.0 30.0 34.0 3 31.377057801247755 31.0 31.0 34.0 28.0 34.0 4 35.179150776607365 35.0 35.0 37.0 33.0 37.0 5 35.05655279819316 35.0 35.0 37.0 32.0 37.0 6 35.090359285039895 36.0 35.0 37.0 32.0 37.0 7 34.97133309883925 36.0 35.0 37.0 32.0 37.0 8 34.975036793972265 36.0 35.0 37.0 32.0 37.0 9 36.44483449654739 38.0 35.0 39.0 32.0 39.0 10-11 36.381674114167765 38.0 35.0 39.0 32.0 39.0 12-13 36.251800359146195 38.0 35.0 39.0 32.0 39.0 14-15 37.25529607577244 39.0 36.0 40.0 32.0 41.0 16-17 37.201363980321005 39.0 36.0 40.0 32.0 41.0 18-19 37.23866791935872 39.0 36.0 40.0 31.0 41.0 20-21 37.21703555130792 39.0 36.0 40.0 31.0 41.0 22-23 37.10660532795231 39.0 36.0 40.0 31.0 41.0 24-25 36.97264171464539 39.0 36.0 40.0 31.0 41.0 26-27 36.69288111844419 39.0 35.5 40.0 30.0 41.0 28-29 36.48765377103504 38.0 35.0 40.0 30.0 41.0 30-31 36.239357806338745 38.0 35.0 40.0 30.0 41.0 32-33 36.0538959378529 38.0 34.5 40.0 30.0 41.0 34-35 36.125814847640555 38.0 35.0 40.0 30.0 41.0 36-37 36.1957416183794 38.0 35.0 40.0 30.0 41.0 38-39 36.168354406945966 38.0 35.0 40.0 30.0 41.0 40-41 36.0396272816891 38.0 35.0 40.0 29.5 41.0 42-43 35.884373206583106 38.0 35.0 40.0 29.0 41.0 44-45 35.690702984708516 38.0 34.5 40.0 28.5 41.0 46-47 35.41906830257142 38.0 34.0 40.0 27.0 41.0 48-49 35.1714978039321 38.0 34.0 40.0 26.5 41.0 50-51 34.89594479515708 38.0 33.5 40.0 25.5 41.0 52-53 34.67635275700243 38.0 33.0 40.0 24.5 41.0 54-55 34.51056843216024 38.0 33.0 40.0 24.5 41.0 56-57 34.31562633059963 38.0 33.0 40.0 24.5 41.0 58-59 34.08898037886221 37.0 33.0 40.0 24.0 41.0 60-61 33.774152986559784 37.0 32.0 40.0 23.0 41.0 62-63 33.515469088435125 37.0 32.0 39.5 23.0 41.0 64-65 33.185089011329765 36.0 31.5 39.0 22.0 40.5 66-67 32.851963849343726 36.0 31.0 39.0 22.0 40.0 68-69 32.4954944739619 35.5 31.0 39.0 21.5 40.0 70-71 32.11071572375363 35.0 31.0 38.0 20.0 40.0 72-73 31.66673946396505 35.0 30.5 37.5 19.5 40.0 74-75 31.1431453292482 34.0 30.0 37.0 18.0 39.0 76-77 29.83008676666605 33.0 28.5 35.5 15.5 38.5 78-79 30.558734113797506 34.0 29.5 36.0 18.0 39.0 80-81 30.518371820445417 34.0 30.0 36.0 18.0 38.0 82-83 30.245073599514967 34.0 30.0 35.5 16.5 37.0 84-85 29.88399109308181 34.0 29.0 35.0 12.0 37.0 86-87 29.51905852555677 34.0 29.0 35.0 7.5 36.0 88-89 29.150370195956828 34.0 29.0 35.0 7.0 36.0 90-91 28.779148809634002 34.0 29.0 35.0 2.0 36.0 92-93 28.42281480830109 33.0 29.0 35.0 2.0 35.0 94-95 27.99635936371883 33.0 27.5 35.0 2.0 35.0 96-97 27.514627918062832 33.0 27.0 35.0 2.0 35.0 98-99 26.95635922487365 33.0 26.0 35.0 2.0 35.0 100-101 25.74606981366977 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25272469951411125 1101 2 0.3850146955496889 1101 3 0.3425574196396397 1101 4 0.3204555662719457 1101 5 0.3022117197748031 1101 6 0.3825253709350136 1101 7 0.3514255939502604 1101 8 0.392677399193893 1101 9 0.3937097957530611 1101 10-11 0.4178514084956646 1101 12-13 0.3517916692276799 1101 14-15 0.4621829931115329 1101 16-17 0.5386666409590077 1101 18-19 0.5843251996843861 1101 20-21 0.5208294721533733 1101 22-23 0.5840171326250854 1101 24-25 0.5264450362082513 1101 26-27 0.46432604252140663 1101 28-29 0.5584041968711233 1101 30-31 0.43272927897005076 1101 32-33 0.38747291212867196 1101 34-35 0.4692454586764043 1101 36-37 0.5801493997834299 1101 38-39 0.540485851081904 1101 40-41 0.5972822647551439 1101 42-43 0.5938232430030439 1101 44-45 0.5930267842052928 1101 46-47 0.38364079940098605 1101 48-49 0.383516442844936 1101 50-51 0.30800246792195907 1101 52-53 0.30968518915241816 1101 54-55 0.298289940732289 1101 56-57 0.45432861138063885 1101 58-59 0.41183264395673547 1101 60-61 0.3401471659048454 1101 62-63 0.28799624375608346 1101 64-65 0.5061694882938781 1101 66-67 0.4057556964834603 1101 68-69 0.1984113253561759 1101 70-71 0.3446273293422166 1101 72-73 0.3914432847780205 1101 74-75 0.29229735265756673 1101 76-77 0.20747027784239336 1101 78-79 0.5197153389102418 1101 80-81 0.5080597363138146 1101 82-83 0.5268868804911477 1101 84-85 0.4660174534395658 1101 86-87 0.5427732538642118 1101 88-89 0.46352156944129064 1101 90-91 0.43837675020665756 1101 92-93 0.3409879177492918 1101 94-95 0.4283573510861274 1101 96-97 0.683644198785256 1101 98-99 0.6460406651434276 1101 100-101 0.6991109900237547 1102 1 0.3636947050880188 1102 2 0.44130201211587305 1102 3 0.40069580826984463 1102 4 0.14336784663208846 1102 5 0.27839970399261915 1102 6 0.34496236024723004 1102 7 0.3792920782277065 1102 8 0.3225125186920934 1102 9 0.40416208531379283 1102 10-11 0.47998645765069625 1102 12-13 0.5293704523638141 1102 14-15 0.4857391325613918 1102 16-17 0.5867300130987303 1102 18-19 0.5816073123162155 1102 20-21 0.5258767164778462 1102 22-23 0.5394073303405165 1102 24-25 0.6037160375396624 1102 26-27 0.6086256898319391 1102 28-29 0.4441853833584233 1102 30-31 0.3356398798565223 1102 32-33 0.5633454473793691 1102 34-35 0.45129669235934244 1102 36-37 0.6038092893276072 1102 38-39 0.5580929418684022 1102 40-41 0.5042369895526946 1102 42-43 0.5673837028380646 1102 44-45 0.6894708638172204 1102 46-47 0.6507264014436132 1102 48-49 0.6459250758263835 1102 50-51 0.378860131311626 1102 52-53 0.36617757188743383 1102 54-55 0.47955322135286593 1102 56-57 0.45589733714692926 1102 58-59 0.4779941703792261 1102 60-61 0.40683565761578677 1102 62-63 0.24615179222593753 1102 64-65 0.14393105414750096 1102 66-67 0.07883732377174368 1102 68-69 0.32243315364581804 1102 70-71 0.43523341244451785 1102 72-73 0.3877186259667482 1102 74-75 0.502952943722768 1102 76-77 0.47947990195671863 1102 78-79 0.7092482018286823 1102 80-81 0.7202232026501001 1102 82-83 0.48117294597114935 1102 84-85 0.6353595135452501 1102 86-87 0.5301452548475574 1102 88-89 0.5893454851463211 1102 90-91 0.5997568272677256 1102 92-93 0.6838132184115899 1102 94-95 0.6603300876302605 1102 96-97 0.9470672095217161 1102 98-99 1.0888783184562492 1102 100-101 1.246610886577912 1103 1 0.3040666963352834 1103 2 0.38495627907429864 1103 3 0.3306739264317713 1103 4 0.24128907201501448 1103 5 0.26527060114797507 1103 6 0.2650936512756772 1103 7 0.39843868654280357 1103 8 0.423716019786184 1103 9 0.6098513632131386 1103 10-11 0.4080499149811132 1103 12-13 0.45743390969423103 1103 14-15 0.4835509487539511 1103 16-17 0.5199904069718215 1103 18-19 0.49900337358536007 1103 20-21 0.5483056005041078 1103 22-23 0.45707691458559196 1103 24-25 0.4765278537322146 1103 26-27 0.5312186876437508 1103 28-29 0.6263516853277835 1103 30-31 0.41797029561144683 1103 32-33 0.6112119681671047 1103 34-35 0.7477955982674374 1103 36-37 0.73756202455737 1103 38-39 0.6551935983235424 1103 40-41 0.6825739698590425 1103 42-43 0.709615650321652 1103 44-45 0.6571951526574793 1103 46-47 0.5653872329534622 1103 48-49 0.5884852508810923 1103 50-51 0.7396369365632651 1103 52-53 0.6402475937692671 1103 54-55 0.6193234620530816 1103 56-57 0.675262763842774 1103 58-59 0.6855981091100816 1103 60-61 0.5999428786223504 1103 62-63 0.4827491664053625 1103 64-65 0.4888435267952005 1103 66-67 0.7238045010146337 1103 68-69 0.35689704861299276 1103 70-71 0.43331875161301525 1103 72-73 0.5417120614153248 1103 74-75 0.5086969262173007 1103 76-77 0.6509788078648135 1103 78-79 0.7705173484369965 1103 80-81 0.7054529619498844 1103 82-83 0.7355493135860307 1103 84-85 0.6389153122323421 1103 86-87 0.687420966007295 1103 88-89 0.6779669293476296 1103 90-91 0.7586736762830455 1103 92-93 0.6088679230067733 1103 94-95 0.5380653173895595 1103 96-97 0.6751853714473164 1103 98-99 0.8432546860711305 1103 100-101 1.3166327684159889 1104 1 0.29529703915074634 1104 2 0.3326551849823929 1104 3 0.2821899001735666 1104 4 0.19577718288299906 1104 5 0.2323328914470295 1104 6 0.20595258635955815 1104 7 0.26595670258948445 1104 8 0.35409734728435893 1104 9 0.4335359729870234 1104 10-11 0.39101373700882647 1104 12-13 0.36025885498963106 1104 14-15 0.48433709025650984 1104 16-17 0.4729281166727688 1104 18-19 0.3613819293840521 1104 20-21 0.41757489299134676 1104 22-23 0.44979318008522284 1104 24-25 0.38522471733542574 1104 26-27 0.5620512186430204 1104 28-29 0.5396044934970021 1104 30-31 0.44381522631895365 1104 32-33 0.5270393934041593 1104 34-35 0.5433006310901973 1104 36-37 0.5299586693422 1104 38-39 0.7262779163395905 1104 40-41 0.5290568290858815 1104 42-43 0.617707262283723 1104 44-45 0.8003527018916117 1104 46-47 0.4811234838652041 1104 48-49 0.6334824791815947 1104 50-51 0.420129423798862 1104 52-53 0.29115437713907255 1104 54-55 0.5348658398794939 1104 56-57 0.6793829680732628 1104 58-59 0.8631416539678511 1104 60-61 0.7822325941584509 1104 62-63 0.6913409971858187 1104 64-65 0.5339075676413003 1104 66-67 0.47952951924949616 1104 68-69 0.6282747291381554 1104 70-71 0.5615579930426264 1104 72-73 0.39475407454443 1104 74-75 0.5413113682304278 1104 76-77 0.6696567072083575 1104 78-79 0.7720511194070916 1104 80-81 0.5293536184050218 1104 82-83 0.5527178037392027 1104 84-85 0.6227269825459807 1104 86-87 0.8793071804493096 1104 88-89 0.8967921664008749 1104 90-91 0.7483361124610148 1104 92-93 0.8112762381052399 1104 94-95 0.7602960978417812 1104 96-97 0.8280863196603008 1104 98-99 0.9702816736714261 1104 100-101 1.2605046867237917 1105 1 0.17734027863632207 1105 2 0.2966793768489211 1105 3 0.24303077608597334 1105 4 0.13491572998066914 1105 5 0.1910993843215536 1105 6 0.3222225898353983 1105 7 0.2887351523635573 1105 8 0.28003631148317965 1105 9 0.47839547941732974 1105 10-11 0.4944058816038961 1105 12-13 0.3520294493587741 1105 14-15 0.3465638542467744 1105 16-17 0.39499582609229833 1105 18-19 0.5147875602495375 1105 20-21 0.4433557758554372 1105 22-23 0.3167142784515775 1105 24-25 0.5040287493131856 1105 26-27 0.4028656781494533 1105 28-29 0.4454386262742034 1105 30-31 0.3514374717134672 1105 32-33 0.456023474043711 1105 34-35 0.5694845726960338 1105 36-37 0.5652907513720891 1105 38-39 0.3646480240907195 1105 40-41 0.3830762938060772 1105 42-43 0.4054364246055897 1105 44-45 0.701055517324356 1105 46-47 0.5653039496281522 1105 48-49 0.4303005535646349 1105 50-51 0.41207506161602936 1105 52-53 0.36290328224855983 1105 54-55 0.4569954193025012 1105 56-57 0.4584842541351364 1105 58-59 0.4466465549063301 1105 60-61 0.4012140331574088 1105 62-63 0.3977633816140411 1105 64-65 0.46566509284116364 1105 66-67 0.3898917020788346 1105 68-69 0.2108449064061375 1105 70-71 0.22237064511075033 1105 72-73 0.2982552215725818 1105 74-75 0.3894369156756845 1105 76-77 0.49288965819897257 1105 78-79 0.5047853564595748 1105 80-81 0.29942535980231355 1105 82-83 0.42482207902595803 1105 84-85 0.43641846673089546 1105 86-87 0.5212216747141696 1105 88-89 0.608216288750544 1105 90-91 0.6365055945604254 1105 92-93 0.6755752996276598 1105 94-95 0.6349186908205837 1105 96-97 0.7436802021768116 1105 98-99 0.9226459420064934 1105 100-101 0.9075673214127029 1106 1 0.192345814443609 1106 2 0.1841175917981559 1106 3 0.17947893105748491 1106 4 0.04822787510877191 1106 5 0.11047815236289438 1106 6 0.1820778259761724 1106 7 0.27785787404848605 1106 8 0.1704389873269605 1106 9 0.442317229643038 1106 10-11 0.23275431208870856 1106 12-13 0.19638026074043324 1106 14-15 0.3972176014386193 1106 16-17 0.16626315394645985 1106 18-19 0.3431561572860815 1106 20-21 0.22571248617557416 1106 22-23 0.22159722694357953 1106 24-25 0.2414807343748322 1106 26-27 0.3242369481424845 1106 28-29 0.29308010585057076 1106 30-31 0.23884653622311447 1106 32-33 0.3659003092721065 1106 34-35 0.41635537017432966 1106 36-37 0.30547815816009205 1106 38-39 0.4807567235813437 1106 40-41 0.3205349973499949 1106 42-43 0.21626700669266796 1106 44-45 0.30679998623665483 1106 46-47 0.1503890855691452 1106 48-49 0.3543122981379341 1106 50-51 0.31824784765402825 1106 52-53 0.221230202485188 1106 54-55 0.29177489206266216 1106 56-57 0.20795676999019008 1106 58-59 0.3326786081744544 1106 60-61 0.30593568255036274 1106 62-63 0.09365239228699096 1106 64-65 0.15448775933458592 1106 66-67 0.0791929910279876 1106 68-69 0.20297206673133417 1106 70-71 0.21869963308522244 1106 72-73 0.13967377635486145 1106 74-75 0.3523128591782445 1106 76-77 0.20513956130846722 1106 78-79 0.12412225891720752 1106 80-81 0.19925223395348013 1106 82-83 0.3665175907466569 1106 84-85 0.29881090160881385 1106 86-87 0.24322198343120505 1106 88-89 0.22559983413250606 1106 90-91 0.26647796549615776 1106 92-93 0.2715837993406005 1106 94-95 0.35556169954572425 1106 96-97 0.3199235933557212 1106 98-99 0.3461751773987949 1106 100-101 0.7507862628686262 1107 1 0.24070678910577215 1107 2 0.28264244887877865 1107 3 0.1801223340645528 1107 4 0.09488623127299434 1107 5 0.17414803805613133 1107 6 0.22545422334462728 1107 7 0.3187164075723956 1107 8 0.22710126885299076 1107 9 0.13639504836029204 1107 10-11 0.27321312538803255 1107 12-13 0.2528309003215341 1107 14-15 0.4654359929368397 1107 16-17 0.35693056315594873 1107 18-19 0.3469037858330566 1107 20-21 0.3565004551428075 1107 22-23 0.37822347330342865 1107 24-25 0.42325788876914316 1107 26-27 0.555311589213261 1107 28-29 0.5664801186094408 1107 30-31 0.4627910133310067 1107 32-33 0.5288602964442788 1107 34-35 0.3457597784188593 1107 36-37 0.471249692907584 1107 38-39 0.35093227863497134 1107 40-41 0.5642768326836531 1107 42-43 0.5690304499073946 1107 44-45 0.633168675987676 1107 46-47 0.5710897367883945 1107 48-49 0.5599432095287824 1107 50-51 0.4895374468245848 1107 52-53 0.5613445552286578 1107 54-55 0.6415479049991148 1107 56-57 0.6350305305845083 1107 58-59 0.5508752751227348 1107 60-61 0.4867087949139872 1107 62-63 0.12947778437776236 1107 64-65 0.20095358311170486 1107 66-67 0.4021422193754205 1107 68-69 0.37765497738423903 1107 70-71 0.35775083348155334 1107 72-73 0.3860110632114555 1107 74-75 0.6126663601354458 1107 76-77 0.5398654497879569 1107 78-79 0.5861055896355474 1107 80-81 0.5649659227763131 1107 82-83 0.6120896332049313 1107 84-85 0.5896125375019103 1107 86-87 0.5243865112642041 1107 88-89 0.6283054601587459 1107 90-91 0.6617110360084197 1107 92-93 0.6911885964430731 1107 94-95 0.76290563193065 1107 96-97 0.6697397105579448 1107 98-99 0.7214210872350115 1107 100-101 0.8795956023561011 1108 1 0.007894612312771443 1108 2 0.07599479954342314 1108 3 -0.02112230539388804 1108 4 0.017812015145665328 1108 5 -0.026249835176813008 1108 6 0.0683468761525603 1108 7 0.07297797311054666 1108 8 0.06829663350848847 1108 9 0.008939541866396894 1108 10-11 0.009139789828246592 1108 12-13 -0.007452065781244244 1108 14-15 0.12187684757270745 1108 16-17 0.10942304886405907 1108 18-19 0.07365816064743314 1108 20-21 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-1.3955623982884156 2316 98-99 -1.5156574288926805 2316 100-101 -1.8220267235326197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 9.0 4 22.0 5 59.0 6 107.0 7 314.0 8 825.0 9 1269.0 10 2408.0 11 3696.0 12 5095.0 13 6641.0 14 8505.0 15 10666.0 16 12736.0 17 15077.0 18 12918.0 19 10102.0 20 9971.0 21 10614.0 22 11414.0 23 13559.0 24 16741.0 25 20854.0 26 26805.0 27 34132.0 28 42744.0 29 52755.0 30 63520.0 31 76443.0 32 91404.0 33 109733.0 34 134279.0 35 165137.0 36 212465.0 37 277259.0 38 232989.0 39 35269.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.55533185226326 22.782097565491068 15.392310006479681 22.27026057576599 2 15.557664047765062 22.557594621802064 40.296101732177775 21.588639598255092 3 18.686086793526933 27.14444066470892 31.133064432148732 23.036408109615415 4 12.543480112574596 18.746382089583374 39.36605228101466 29.344085516827377 5 11.693212697431946 39.254859829899445 36.449837923711044 12.602089548957569 6 28.811392306201494 40.562730867468694 17.654487759866015 12.971389066463798 7 25.296434455819465 33.69575781698354 23.329923912842254 17.67788381435474 8 23.947860778489883 38.27428687795266 20.398825357558984 17.379026985998475 9 24.76176481478053 16.675768739470907 21.372438306459077 37.19002813928948 10-11 23.50032744321232 27.944790528907564 29.22372817816613 19.331153849713978 12-13 24.132073004794787 25.33325735416628 29.670202204861436 20.8644674361775 14-15 21.387566645685617 27.068243562211897 27.243043856563677 24.301145935538813 16-17 20.36705458466779 30.362287566877093 28.125404965103577 21.145252883351535 18-19 20.286119416109745 28.880462400725698 30.699912758946258 20.1335054242183 20-21 20.986709045300554 28.091821787585392 30.88657274561712 20.034896421496935 22-23 20.74757331261449 27.829418997945954 30.715265691064626 20.707741998374928 24-25 20.56747641059166 28.197485724864478 30.788155253307725 20.446882611236138 26-27 20.47294568772903 28.350967262679593 30.754949707431823 20.421137342159554 28-29 20.404630731502152 28.330312477763016 30.883306653769456 20.38175013696538 30-31 20.843409950336262 28.0986320486177 30.74725877446738 20.310699226578663 32-33 20.70094193430416 28.37182159377738 30.461951710098568 20.465284761819895 34-35 20.656165966796806 28.311879175894195 30.764581907918053 20.267372949390946 36-37 20.8111757518536 28.229800153656754 30.701585395203317 20.25743869928633 38-39 20.598823111173846 28.389731105519367 30.755918070765482 20.2555277125413 40-41 20.736795579087676 28.2427891735782 30.713671643587087 20.306743603747037 42-43 20.640392008016978 28.325287039758567 30.41646512359894 20.61785582862552 44-45 20.636576444600273 28.557766075253326 30.266107074627314 20.539550405519087 46-47 20.79978384064149 28.446623652728647 29.989391643901758 20.764200862728103 48-49 20.870080660419116 28.629415103476884 29.79468362149718 20.70582061460682 50-51 20.94133349251434 28.79352581485124 29.48179048495537 20.783350207679057 52-53 20.915787074828554 28.98748219508826 29.421662876683012 20.67506785340017 54-55 20.88631747114605 28.96038980933956 29.522496867458862 20.630795852055527 56-57 20.922774467750276 28.935708838949836 29.353287225659535 20.788229467640353 58-59 20.809169453017027 28.951494213832586 29.561718685130018 20.677617648020373 60-61 20.86626486624865 29.078857289804827 29.494394061724975 20.560483782221546 62-63 20.800127325531843 28.906925384107385 29.62510284319768 20.667844447163095 64-65 20.655129825700737 28.78753000661015 29.60823253818232 20.94910762950679 66-67 20.72238544756137 28.81565285415928 29.490596799713764 20.971364898565593 68-69 20.735061018304478 28.88003555764824 29.51696512108997 20.86793830295731 70-71 20.84463818718026 28.64118241692871 29.539144629751117 20.97503476613991 72-73 20.875646098322246 28.973276544856503 29.3198129479813 20.831264408839953 74-75 20.763047596509118 29.14185567105985 29.191796681503963 20.903300050927072 76-77 20.658122831470692 29.01295487488142 29.35310837871467 20.97581391493322 78-79 20.612004678221822 29.037548139660487 29.277881227202997 21.072565954914694 80-81 20.779060746536246 29.0696557736776 29.1004620654505 21.05082141433566 82-83 20.869789834681672 29.031861497306405 29.29841531369754 20.799933354314383 84-85 20.84485555473277 29.088672316122704 29.059948719847455 21.006523409297074 86-87 20.6556211470463 29.10518910713294 29.212504859581244 21.026684886239515 88-89 20.806581500478437 29.09530112661422 29.078813242605616 21.019304130301723 90-91 20.781172481923598 28.945848177027962 29.308113878077037 20.9648654629714 92-93 20.82817625434916 29.04513174637066 29.10545438133279 21.021237617947385 94-95 20.55591794584009 29.004088823064027 29.142157110892875 21.29783612020301 96-97 20.588923564839416 28.86473496327978 29.273865540554805 21.272475931326 98-99 20.50393278967732 28.94699238202788 29.161450330451526 21.387624497843273 100-101 20.6147485976637 28.933339272821517 29.033192096932446 21.418720032582335 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 311.0 1 206.5 2 148.5 3 220.5 4 218.5 5 212.5 6 337.5 7 711.5 8 1251.5 9 1567.5 10 1442.5 11 1210.5 12 1211.0 13 1356.5 14 1534.5 15 1743.5 16 1976.0 17 2218.0 18 2484.0 19 2800.5 20 3188.0 21 3674.5 22 4504.5 23 5799.0 24 7223.0 25 8769.0 26 11267.5 27 14455.5 28 17693.5 29 21771.0 30 26362.0 31 30925.0 32 37372.0 33 44405.0 34 50200.0 35 55423.5 36 62205.0 37 69132.0 38 71624.0 39 72404.0 40 72739.0 41 71599.0 42 70092.5 43 70490.5 44 71842.5 45 72380.0 46 70654.5 47 67940.0 48 66469.5 49 64451.0 50 61333.0 51 56867.5 52 51100.0 53 45057.0 54 39573.0 55 33587.5 56 26867.5 57 20646.5 58 15955.5 59 12212.0 60 8885.5 61 6192.0 62 4188.5 63 2647.0 64 1534.0 65 860.0 66 457.5 67 225.0 68 113.0 69 69.0 70 40.5 71 22.0 72 15.0 73 12.0 74 7.5 75 4.5 76 2.5 77 2.5 78 1.5 79 0.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0037025380898606 2 0.004859581242942037 3 0.0016198604143140124 4 0.159382694336968 5 0.002140529833200659 6 5.206694188866468E-4 7 0.0 8 0.0010413388377732936 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.603347094433234E-4 24-25 8.099302071570062E-4 26-27 0.003355425143936168 28-29 2.8926078827035937E-4 30-31 0.0069422589184886235 32-33 0.023025158746320603 34-35 0.014260556861728715 36-37 9.2563452246515E-4 38-39 2.0248255178925156E-4 40-41 0.0018512690449303 42-43 0.012264657422663236 44-45 0.008215006386878205 46-47 0.010962983875446619 48-49 0.010818353481311438 50-51 0.17615982005664885 52-53 0.10665045263528149 54-55 0.018194503582205604 56-57 0.007289371864413055 58-59 0.018946581631708537 60-61 0.11466297647037044 62-63 0.22163161597274933 64-65 0.227011866634578 66-67 0.23745418109113797 68-69 0.23259459984819592 70-71 0.2201563859525705 72-73 0.15082057500416537 74-75 0.26091323101986413 76-77 0.19904034840883428 78-79 0.00633481126312087 80-81 5.785215765407187E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.1570431530814375E-4 90-91 0.0067397763666993725 92-93 0.01949617712942222 94-95 0.0029793861191847013 96-97 2.8926078827035937E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1728544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.495651801032686 #Duplication Level Percentage of deduplicated Percentage of total 1 76.36401763945184 43.90598968325841 2 14.244200598472887 16.37959195587717 3 4.214865752985442 7.270093611652452 4 1.7699384253136579 4.070550536444087 5 0.9129518218052751 2.624538002881727 6 0.538981017808652 1.8593438956375477 7 0.348596854323994 1.4029962348603375 8 0.24091588274237546 1.1081292565995233 9 0.19207609889321367 0.993918645113844 >10 1.038137234442858 11.282274153930052 >50 0.0883327234363507 3.4923068155664794 >100 0.04577618123921682 4.464034677651997 >500 8.065127228532456E-4 0.32401718562033255 >1k 3.024422710699671E-4 0.5245716511384632 >5k 1.008140903566557E-4 0.29764369376751476 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5135 0.29707082955365904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4828 0.27931021715385895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2987 0.17280439491271266 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.67782364811078E-5 0.0 24-25 0.0 0.0 0.0 1.1570431530814373E-4 0.0 26-27 0.0 0.0 0.0 1.4463039413517966E-4 0.0 28-29 0.0 0.0 0.0 5.206694188866468E-4 0.0 30-31 0.0 0.0 0.0 0.001677712571968084 0.0 32-33 0.0 0.0 0.0 0.005177768110039432 0.0 34-35 0.0 0.0 0.0 0.010239831904770721 0.0 36-37 0.0 0.0 0.0 0.019756511838865544 0.0 38-39 0.0 0.0 0.0 0.03450881204065387 0.0 40-41 0.0 2.8926078827035933E-5 0.0 0.051141307366199526 0.0 42-43 0.0 5.7852157654071866E-5 0.0 0.07026144547087028 0.0 44-45 2.8926078827035933E-5 5.7852157654071866E-5 0.0 0.09398083010903974 0.0 46-47 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.12331187403965418 0.0 48-49 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.15452311309402594 0.0 50-51 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.18816414276986876 0.0 52-53 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.22171839420923042 0.0 54-55 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.26461576910972473 0.0 56-57 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.3137033248792047 0.0 58-59 5.7852157654071866E-5 5.7852157654071866E-5 0.0 0.36938602662124886 0.0 60-61 5.7852157654071866E-5 8.67782364811078E-5 0.0 0.43418044319380933 0.0 62-63 5.7852157654071866E-5 1.1570431530814373E-4 0.0 0.5037187366940037 0.0 64-65 5.7852157654071866E-5 1.1570431530814373E-4 0.0 0.5734305866671603 0.0 66-67 5.7852157654071866E-5 1.1570431530814373E-4 0.0 0.6581550715515486 0.0 68-69 5.7852157654071866E-5 1.1570431530814373E-4 0.0 0.7528012014736102 0.0 70-71 5.7852157654071866E-5 1.1570431530814373E-4 0.0 0.8598855452912972 0.0 72-73 5.7852157654071866E-5 1.1570431530814373E-4 0.0 0.9726972527167373 0.0 74-75 5.7852157654071866E-5 1.1570431530814373E-4 0.0 1.0894718329414812 0.0 76-77 5.7852157654071866E-5 1.1570431530814373E-4 0.0 1.2243541385119499 0.0 78-79 5.7852157654071866E-5 1.1570431530814373E-4 0.0 1.3883071533035896 0.0 80-81 5.7852157654071866E-5 1.1570431530814373E-4 0.0 1.5754299572356851 0.0 82-83 5.7852157654071866E-5 1.1570431530814373E-4 0.0 1.7670941555436253 0.0 84-85 5.7852157654071866E-5 1.1570431530814373E-4 0.0 1.9619691486013662 0.0 86-87 5.7852157654071866E-5 1.1570431530814373E-4 0.0 2.1714518114667607 0.0 88-89 5.7852157654071866E-5 1.1570431530814373E-4 0.0 2.407517540774201 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2760 0.0 60.62111 1 GTATCAA 5495 0.0 42.38563 1 ATCAACG 6945 0.0 32.982 3 CAACGCA 6990 0.0 32.71658 5 TCAACGC 7075 0.0 32.307865 4 TATCAAC 7135 0.0 32.106503 2 AACGCAG 7355 0.0 31.2847 6 TACCGGC 115 3.9023053E-6 28.878403 7 GTGGTAT 1420 0.0 27.113586 1 CGCAGAG 8425 0.0 27.0862 8 ACGCAGA 8435 0.0 27.054092 7 TGGTATC 1430 0.0 24.262009 2 GCAGAGT 9430 0.0 23.7467 9 GAGTACT 6560 0.0 20.75635 12-13 AGAGTAC 9060 0.0 20.658165 10-11 CAGAGTA 9355 0.0 20.53923 10-11 GTACATG 5770 0.0 20.512274 1 GAGTACG 255 1.1836164E-8 20.504198 1 ACATGGG 5550 0.0 20.293528 3 TACATGG 5815 0.0 20.105984 2 >>END_MODULE