FastQCFastQC Report
Sat 18 Jun 2016
SRR1931016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352640
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN553664.093180742843624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.12893304944405015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12850.061.455931
GTATCAA25000.045.0388681
TCAACGC29250.037.508114
ATCAACG29600.037.2477463
TATCAAC30100.036.384222
CAACGCA30350.036.14295
AACGCAG32450.033.228636
ACGCAGA36300.029.4342277
CGCAGAG37550.028.8375288
GTACATG20000.025.729221
TAACGCA1601.1696266E-625.0482234
GCAGAGT43050.024.7606169
TACATGG21150.023.6024362
GTGGTAT7000.023.2909491
ACATGGG20500.022.9796263
CCGTGCG1000.002503272422.1150329
CAGAGTA40900.022.00842510-11
TGGTATC6350.021.6586362
GAGTACT30350.021.43668712-13
TATCACG1200.003177564821.2241292
Produced by FastQC (version 0.11.5)